| red drum somatolactin: development of a homologous radioimmunoassay and plasma levels after exposure to stressors or various backgrounds. | a specific radioimmunoassay (ria) for measurement of red drum (sciaenops ocellatus) somatolactin (rdsl) was developed using somatolactin purified from red drum pituitaries and antisera to rdsl raised in rabbits. the rdsl antisera specifically bound to cells in the pars intermedia bordering the neurohypophysis in red drum and atlantic croaker (micropogonias undulatus) pituitaries. these cells correspond to the periodic acid-schiff-positive cells and are distinct from the lead-hematoxylin-positive ... | 1995 | 8536939 |
| major diseases of striped bass and redfish. | diseases of striped bass, their hybrids, and redfish (red drum) are important constraints to the culture of these two species. since striped bass have been cultured for years the organisms that cause most diseases of these fish are well known, but very little specific disease information exists for redfish. however, it appears that the organisms that cause diseases of striped bass and redfish do not differ greatly from those of other fishes. the most significant viral disease is lymphocystis, bu ... | 1991 | 1926745 |
| histochemical and immunocytochemical identification of the pituitary cell types in three sciaenid fishes: atlantic croaker (micropogonias undulatus), spotted seatrout (cynoscion nebulosus), and red drum (sciaenops ocellatus). | the adenohypophysial cell types in three species of sciaenid teleosts were identified using a combination of classical histochemical techniques and immunocytochemistry with antisera raised against piscine and human pituitary hormones. in general greater specificity for the different cell types was observed with antisera to the piscine pituitary hormones than with antisera raised against the human hormones. the distribution of the cell types did not differ significantly among the three species, a ... | 1991 | 1667000 |
| [identification of a vibrio splendidus strain isolated from ill sciaenops ocellatus]. | we isolated bacterial strain named m-1 from ill sciaenops ocellatus with ulceration, and further analyzed the phylogenetics of the bacterial strain. | 2010 | 20560360 |
| identification and molecular analysis of a stress-inducible hsp70 from sciaenops ocellatus. | hsp70 proteins are a family of molecular chaperones that are involved in many aspects of protein homeostasis. in this study, an hsp70 homologue (sohsp70) was identified from red drum sciaenops ocellatus and analyzed at molecular level. the open reading frame of sohsp70 is 1920 bp and intronless, with a 5'-untranslated region (utr) of 399 bp and a 3'-utr of 241 bp. the deduced amino acid sequence of sohsp70 shares 84-92% overall identities with the hsp70s of a number of fish species. in silico an ... | 2010 | 20541016 |
| differential regulation of sciaenops ocellatus viperin expression by intracellular and extracellular bacterial pathogens. | viperin is an antiviral protein that has been found to exist in diverse vertebrate organisms and is involved in innate immunity against the infection of a wide range of viruses. however, it is largely unclear as to the potential role played by viperin in bacterial infection. in this study, we identified the red drum sciaenops ocellatus viperin gene (sovip) and analyzed its expression in relation to bacterial challenge. the complete gene of sovip is 2570 bp in length and contains six exons and fi ... | 2010 | 20420911 |
| identification and analysis of a sciaenops ocellatus isg15 homologue that is involved in host immune defense against bacterial infection. | isg15 is an interferon-stimulated gene that encodes a ubiquitin-like protein. isg15 homologues have been identified in a number of fish species, some of which are known to be regulated at expression level by virus infection and lipopolysaccharide (lps) treatment. however, the relationship between isg15 and live bacterial infection has not been investigated in piscine models. in this study, an isg15 homologue, soisg15, was identified from red drum sciaenops ocellatus and analyzed at expression an ... | 2010 | 20385242 |
| production of an anti-idiotypic antibody single chain variable fragment vaccine against edwardsiella tarda. | edwardsiella tarda is the pathogen responsible for edwardsiellosis, a serious infectious disease of freshwater and marine fish species, and currently recognized to be the species pathogenic for human. an anti-idiotypic monoclonal antibody (mab), 1e11, has been developed. it mimics the protective epitope of e. tarda and can prevent fish from infection of e. tarda. in this study, the correct variable heavy (vh) and variable light (vl) genes were obtained from 1e11 by using bioinformatics methods, ... | 2010 | 20119624 |
| identification and molecular analysis of a ferritin subunit from red drum (sciaenops ocellatus). | ferritin is a conserved iron binding protein existing ubiquitously in prokaryotes and eukaryotes. in this study, the gene encoding a ferritin m subunit homologue (sofer1) was cloned from red drum (sciaenops ocellatus) and analyzed at expression and functional levels. the open reading frame of sofer1 is 531 bp and preceded by a 5'-untranslated region that contains a putative iron regulatory element (ire) preserved in many ferritins. the deduced amino acid sequence of sofer1 possesses both the fer ... | 2010 | 20064620 |
| cholecystokinin: molecular cloning and immunohistochemical localization in the gastrointestinal tract of larval red drum, sciaenops ocellatus (l.). | the current study sought to clarify the role of cholecystokinin (cck) in the digestion of larval red drum (sciaenops ocellatus) in order to better characterize the processes limiting the utilization of microparticulate diets at first feeding. the red drum cck cdna, isolated from adult anterior intestine and pyloric caeca, contains a 414 base pair (bp) open reading frame encoding a deduced amino acid sequence of 138 residues which is highly similar to preprocholecystokinin from other vertebrates. ... | 2010 | 19896946 |
| enantiomeric signatures of chiral organochlorine pesticides in consumer fish from south china. | despite the importance of chiral organochlorine pesticide enantiomers in terms of environmental effects, no relevant data have been acquired in consumer fish. the present study determined the residual levels and enantiomer fractions (efs) of α-hch, o,p'-ddt, and o,p'-ddd in 11 consumer fish species from south china, including 6 freshwater farmed fish, 3 seawater farmed fish, and 2 wild marine fish. the mean concentrations of α-hch, o,p'-ddt, and o,p'-ddd were 0.27, 4.4, and 3.5 ng/g wet, respect ... | 2009 | 19354295 |
| comparative visual function in five sciaenid fishes inhabiting chesapeake bay. | maintaining optimal visual performance is a difficult task in the photodynamic coastal and estuarine waters in which western north atlantic sciaenid fishes support substantial commercial and recreational fisheries. unavoidable tradeoffs exist between visual sensitivity and resolution, yet sciaenid visual systems have not been characterized despite strong species-specific ecomorphological and microhabitat differentiation. we therefore used electroretinographic techniques to describe the light sen ... | 2008 | 18978225 |
| variation in fatty acid composition among nine forage species from a southeastern us estuarine and nearshore coastal ecosystem. | the fatty acid (fa) composition of nine potentially important forage species was determined (n = 330): red drum (sciaenops ocellatus), spot (leiostomus xanthurus), spotted seatrout (cynoscion nebulosus), striped mullet (mugil cephalus), pinfish (lagodon rhomboides), atlantic croaker (micropogonias undulatus), star drum (stellifer lanceolatus), striped anchovy (anchoa hepsetus), and brief squid (lolliguncula brevis). samples were collected from estuarine and nearshore coastal waters around charle ... | 2008 | 18665465 |
| acoustic pressure and particle motion thresholds in six sciaenid fishes. | sciaenid fishes are important models of fish sound production, but investigations into their auditory abilities are limited to acoustic pressure measurements on five species. in this study, we used auditory brainstem response (abr) to assess the pressure and particle acceleration thresholds of six sciaenid fishes commonly found in chesapeake bay, eastern usa: weakfish (cynoscion regalis), spotted seatrout (cynoscion nebulosus), atlantic croaker (micropogonias undulatus), red drum (sciaenops ocel ... | 2008 | 18424685 |
| a preliminary in vitro assessment of grobiotic-a, brewer's yeast and fructooligosaccharide as prebiotics for the red drum sciaenops ocellatus. | this study examined the effects of brewers yeast, fructooligosaccharide (fos), and grobiotic-a, a mixture of partially autolyzed brewers yeast, dairy components and dried fermentation products, on the intestinal microbial community of red drum, sciaenops ocellatus. gastrointestinal (gi) tracts were aseptically removed from three sub-adult red drum previously maintained on a commercial diet and placed in an anaerobic chamber. intestinal contents were removed, diluted and incubated in vitro in one ... | 2008 | 18368546 |
| central regulation of thyroidal status in a teleost fish: nutrient stimulation of t4 secretion and negative feedback of t3. | several experiments were conducted to investigate the dynamics of central regulation of thyroid function in the red drum, sciaenops ocellatus, by manipulating a well-characterized circadian rhythm of t(4) secretion. in the first experiment, red drum were reared under either a long (16l:8d) or short (8l:16d) photoperiod and fed at the same time relative to dawn. the same feeding time under different photoperiods maintained the same phase relationship between t(4) cycles under each photoperiod. th ... | 2003 | 12840837 |
| developmental expression of the g protein-coupled receptor 54 and three gnrh mrnas in the teleost fish cobia. | the cdnas of the g protein-coupled receptor 54 (gpr54) and three prepro-gonadotropin-releasing hormones, gnrh-i (seabream gnrh), gnrh-ii (chicken gnrh-ii), and gnrh-iii (salmon gnrh) were isolated and cloned from the brain of the teleost fish cobia, rachycentron canadum. the cobia gpr54 cdna was 95 and 51-56% identical to those of tilapia and mammalian models respectively. the gnrh cdna sequences of cobia showed strong identities to those of tilapia, atlantic croaker, red drum, and the seabass a ... | 2007 | 17293443 |
| nodavirus infections in israeli mariculture. | viral encephalopathy and retinopathy (ver) infections were diagnosed in five fish species: epinephelus aeneus, dicentrarchus labrax, sciaenops ocellatus, lates calcarifer and mugil cephalus cultured on both the red sea and mediterranean coasts of israel during 1998-2002. spongiform vacuolation of nervous tissue was observed in histological sections of all examined species. with transmission electron microscopy, paracrystalline arrays and pieces of membrane-associated non-enveloped virions measur ... | 2004 | 15291788 |
| fish gambler's tenosynovitis caused by mycobacterium marinum: environmental investigation of a fishing pond in southern taiwan. | we describe a patient with mycobacterium marinum tenosynovitis associated with a fish spine injury acquired at a gambling fishing pond in southern taiwan and identify the source of the infection. m. marinum was isolated from fishing ponds and underground water and wastewater at the site. the isolates shared the same pulsed-field gel electrophoresis pattern as the patient. m. marinum was not detected in 54 samples obtained from 27 fish. mycobacterium gordonae was isolated from 24 samples collecte ... | 2007 | 17572037 |
| molecular cloning and differential expression of three gnrh mrnas in discrete brain areas and lymphocytes in red drum. | three gonadotropin releasing hormones, seabream gnrh (gnrh-i), chicken gnrh-ii (gnrh-ii) and salmon gnrh (gnrh-iii) cdnas were isolated from the brain of the red drum, sciaenops ocellatus. the gnrh cdna sequences of red drum showed more similarity to those of atlantic croaker, sea bass and sea bream. the real-time quantitative rt-pcr study revealed expression of gnrh-i and gnrh iii mrnas in the olfactory bulb plus telencephalon (ob+tel), and preoptic-anterior hypothalamic area (poah), indicating ... | 2006 | 16522721 |
| streptococcus iniae infections in red sea cage-cultured and wild fishes. | streptococcus iniae was isolated from 2 moribund wild red sea fishes, pomadasys stridens (pomadasyidae) and synodus variegatus (synodontidae), both collected in shallow waters along the israeli coast of the gulf of eilat. the site is approximately 2 km from a mariculture cage farm in which streptococcal infections were diagnosed in previous years in the red drum sciaenops ocellatus. this is the first report of s. iniae in red sea fishes. biochemical and molecular similarities between the isolate ... | 2002 | 12113301 |
| habitat-related predation on juvenile wild-caught and hatchery-reared red drum sciaenops ocellatus (linnaeus). | we examined the patterns of habitat-specific mortality for newly settled red drum (sciaenops ocellatus) using an experimental mesocosm approach. experiments were designed to analyze prey vulnerability and fish rearing-type (wild-caught or hatchery-reared) in estuarine habitats of varying structural complexity including marsh (spartina alterniflora loisel), oyster reef (crassostrea virginica gmelin), seagrass (halodule wrightii aschers), and nonvegetated sand bottom. we used two different predato ... | 2001 | 11358569 |
| red drum sciaenops ocellatus mortalities associated with streptococcus iniae infection. | we isolated for the first time streptococcus iniae strains associated with diseased marine fish. diseased red drum sciaenops ocellatus were lethargic, and presented external signs (exophthalmia and loss of orientation) resembling those of freshwater fish infected by s. iniae. skin lesions, extending to a necrotizing myositis, were typical of s. iniae infection of red drum. histopathological findings indicate that s. iniae infection in red drum produces a chronic disease with systemic involvement ... | 1999 | 10399040 |
| molecular cloning and regulation of mrna expression of the thyrotropin β and glycoprotein hormone α subunits in red drum, sciaenops ocellatus. | full-length cdnas for thyrotropin β (tshβ) and glycoprotein hormone α (gsuα) subunits were cloned and sequenced from the red drum (sciaenops ocellatus). the cdnas for tshβ (877 bp) and gsuα (661 bp) yielded predicted coding regions of 126 and 94 amino acid proteins, respectively. both sequences contain all invariant cysteine and putative glycosylated asparagines characteristic of each as deduced by comparison with other gsuα and tshβ sequences from representative vertebrate species. multiple pro ... | 2010 | 20563882 |
| identification and analysis of the tissue factor pathway inhibitor 2 of sciaenops ocellatus. | tissue factor pathway inhibitor 2 (tfpi-2) is a structural homologue of tfpi, a potent inhibitor of tissue factor (tf)-mediated coagulation. although tfpi-2 has been identified at sequence level in several fish species, no study on piscine tfpi-2 has been documented. in this report, we identified and analyzed a tfpi-2 homologue, sotfpi2, from red drum sciaenops ocellatus. the open reading frame of sotfpi2 is 681 bp, which encodes a 226-residue protein that shares 59.2-82.3% overall sequence iden ... | 2010 | 20970505 |
| pathogenesis of gastroenteritis caused by vibrio carchariae in cultured marine fish. | serious mortality among the cultured grouper epinephelus coioides, characterized by a swollen intestine containing yellow fluid (gastroenteritis), occurred in 1993 in taiwan. a bacterium isolated from the intestinal fluid and head kidney of moribund groupers was identified as vibrio carchariae. since then, the same vibrio species has also been isolated from moribund black sea bream acanthopagrus schlegeli, yellowfin sea bream a. latus, japanese sea bass lateolabrax japonicus, and red drum sciaen ... | 2002 | 14961260 |
| interrenal and thyroid development in red drum (sciaenops ocellatus): effects of nursery environment on larval growth and cortisol concentration during settlement. | red drum settle into shallow seagrass meadows during the larval stage. day-night cycles in these habitats result in marked diel temperature and dissolved oxygen (do) cycles, and it is possible that extreme fluctuations influence endocrine development and growth of larvae. here, we described red drum interrenal and thyroid ontogeny and determine responses to environmental stimuli with special emphasis on settlement to explore possible role of hormones as mediator of directive environmental factor ... | 2006 | 16343495 |
| A peptidoglycan recognition protein from Sciaenops ocellatus is a zinc amidase and a bactericide with a substrate range limited to Gram-positive bacteria. | Peptidoglycan recognition proteins (PGRPs) are a family of innate immune molecules that recognize bacterial peptidoglycan. PGRPs are highly conserved in invertebrates and vertebrates including fish. However, the biological function of teleost PGRP remains largely uninvestigated. In this study, we identified a PGRP homologue, SoPGLYRP-2, from red drum (Sciaenops ocellatus) and analyzed its activity and potential function. The deduced amino acid sequence of SoPGLYRP-2 is composed of 482 residues a ... | 2012 | 22146700 |
| Sequestration of total and methyl mercury in different subcellular pools in marine caged fish. | Mercury contamination is an important issue in marine fish, and can cause toxicity to human by fish consumption. Many studies have measured the mercury concentrations in fish and estimated the threshold levels of its risk to human, but the mercury sequestration in different subcellular pools of fish is unclear. In this study, we investigated the concentration and distribution of total mercury (THg) and methylmercury (MeHg) in different subcellular fractions in the farmed red seabream, red drum, ... | 2011 | 22056886 |
| the rab1 gtpase of sciaenops ocellatus modulates intracellular bacterial infection. | the rab family proteins belong to the ras-like gtpase superfamily and play important roles in intracellular membrane trafficking. to date no studies on fish rab have been documented, though rab-like sequences have been found in a number of teleosts. in this study, we identified and analyzed a rab homologue, sorab1, from red drum, sciaenops ocellatus. the cdna of sorab1 contains a 5'- untranslated region (utr) of 358 bp, an open reading frame (orf) of 612 bp, and a 3'-utr of 265 bp. the orf encod ... | 2011 | 21889593 |
| oyster reefs as natural breakwaters mitigate shoreline loss and facilitate fisheries. | shorelines at the interface of marine, estuarine and terrestrial biomes are among the most degraded and threatened habitats in the coastal zone because of their sensitivity to sea level rise, storms and increased human utilization. previous efforts to protect shorelines have largely involved constructing bulkheads and seawalls which can detrimentally affect nearshore habitats. recently, efforts have shifted towards "living shoreline" approaches that include biogenic breakwater reefs. our study e ... | 2011 | 21850223 |
| [renal patient's diet: can fish be included?]. | medical and nutritional treatment for renal disease, now a major public health issue, is highly complicated. nutritional therapy must seek to retard renal dysfunction, maintain an optimal nutritional status and prevent the development of underlying pathologies. | 2012 | 23478696 |
| isolation of maturational gonadotropin subunits from spotted seatrout (cynoscion nebulosus) and development of a beta-subunit-directed radioimmunoassay for gonadotropin measurement in sciaenid fishes. | maturational gonadotropin (gth) subunits were isolated from pituitaries of the spotted seatrout (cynoscion nebulosus), a marine perciform teleost, by ethanolic extraction and ion-exchange, gel-filtration, and reverse-phase chromatography. partial amino acid sequencing of the n-terminal regions of the alpha and beta subunits indicated 60-80% identities with various carp and salmon gth subunits. the spotted seatrout gth beta-subunit was used as radioligand in a radioimmunoassay (ria) with atlantic ... | 2006 | 1426954 |
| identification, mrna expression profiling and activity characterization of cathepsin l from red drum (sciaenops ocellatus). | cathepsin l is a cysteine protease with a papain-like structure. it is known to be implicated in multiple processes of mammalian immune response to pathogen infection. in teleost fish, the functionality of cathepsin l is less understood. in this work, we characterized a cathepsin l homologue (designated as socatl) from red drum sciaenops ocellatus, an important farmed fish species in china. socatl possesses a typical domain arrangement characteristic of cathepsin l, which comprises a proregion a ... | 2015 | 26164862 |
| macrophage migration inhibitory factor of sciaenops ocellatus regulates immune cell trafficking and is involved in pathogen-induced immune response. | macrophage migration inhibitory factor (mif) is a multi-functional cytokine involved in immunoregulation and inflammation. in this study, we examined the expression and biological function of a mif, somif, from red drum sciaenops ocellatus. somif is composed of 115 residues and shares 85-99% overall sequence identities with the mif of a number of teleost. somif expression was detected in a wide range of tissues and upregulated by bacterial and viral infection in a time-dependent manner. in head ... | 2013 | 23545285 |
| identification and characterization of a cell surface scavenger receptor cysteine-rich protein of sciaenops ocellatus: bacterial interaction and its dependence on the conserved structural features of the srcr domain. | the scavenger receptor cysteine-rich (srcr) proteins are secreted or membrane-bound receptors with one or multiple srcr domains. members of the srcr superfamily are known to have diverse functions that include pathogen recognition and immunoregulation. in teleost, although protein sequences with srcr structure have been identified in some species, very little functional investigation has been carried out. in this study, we identified and characterized a teleost srcr protein from red drum sciaeno ... | 2013 | 23291106 |
| permanent genetic resources added to molecular ecology resources database 1 december 2011-31 january 2012. | this article documents the addition of 473 microsatellite marker loci and 71 pairs of single-nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: barteria fistulosa, bombus morio, galaxias platei, hematodinium perezi, macrocentrus cingulum brischke (a.k.a. m. abdominalis fab., m. grandii goidanich or m. gifuensis ashmead), micropogonias furnieri, nerita melanotragus, nilaparvata lugens stål, sciaenops ocellat ... | 2012 | 22448966 |
| a new species of homalometron (digenea: apocreadiidae) from fishes in the northern gulf of mexico. | homalometron palmeri n. sp. is described and reported from the following fishes in the northern gulf of mexico: micropogonias undulatus, sciaenops ocellatus, bairdiella chrysoura, pogonias cromis, fundulus grandis, fundulus similis, and eucinostomus argenteus. this species historically has been misidentified as homalometron pallidum by some, and treated as homalometron sp. by others. the new species differs from h. manteri by having a smaller body size, relatively longer postcecal space ranging ... | 2013 | 22924927 |
| environmental distribution and seasonal prevalence of mycobacterium ulcerans in southern louisiana. | mycobacterium ulcerans is an emerging environmental pathogen that causes debilitating, ulcerative disease in humans and other vertebrates. the majority of human cases occur in tropical and temperate regions of africa and australia, and outbreaks of piscine mycobacteriosis caused by m. ulcerans have been reported in disparate geographic locations spanning the globe. while exposure to a natural body of water is the most common risk factor for human infection, the environmental distribution of m. u ... | 2013 | 23396345 |
| isolation and cdna cloning of somatolactin in rabbitfish (siganus guttatus). | we report the isolation and cdna cloning of somatolactin (sl) from rabbitfish, siganus guttatus. rabbitfish sl was isolated from an alkaline extract of the pituitary glands by gel filtration chromatography on sephadex g-100 and reversed-phase high-performance liquid chromatography. sl was monitored by immunoblotting with flounder sl antiserum. the preparation (yield: 0.86 mg/g wet tissues) contained two immunoreactive bands of 24 and 28 kda on sds-page. overlapping partial cdna clones correspond ... | 1999 | 10417242 |
| occurrence of polybrominated diphenyl ethers in fish and shellfish downstream from electronic-waste recycling plants. | we measured 39 polybrominated diphenyl ethers (pbdes) in the muscle tissue of three species of fish (sciaenops ocellatus, sparus macrocephalus, and lateolabraxjaponicus) and four species of shellfish (tegillarca granosa, cyclina sinensis, sinonovacula constricta, and ostrea cucullata) that were collected downstream of electronic-waste recycling plants in taizhou, china. a total of 24 pbde congeners (pbde24) in the samples were detected. the sigma pbde24 (total pbde) ranged from 545.4 to 1688.7 n ... | 2010 | 20608509 |
| the tissue factor pathway inhibitor 1 of sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection. | tissue factor pathway inhibitor 1 (tfpi-1) is a kunitz-type serine protease inhibitor that regulates the activation of tissue factor-induced coagulation. in teleosts, tfpi-1-like sequences have been found to exist in two species (danio rerio and cyprinus carpio); however, the potential function of fish tfpi-1 has not been investigated. in this study, we identified and analyzed a tfpi-1 homologue, sotfpi-1, from red drum (sciaenops ocellatus). the deduced amino acid sequence of sotfpi-1 is 284 re ... | 2010 | 20970444 |
| quantifying the seafood consumption patterns of recreational anglers in charleston and berkeley counties, south carolina. | this study was designed to provide self-reported data on the frequency of fish consumption and shellfish consumption in charleston and berkeley (cb) counties, south carolina. while commercial fishing and recreational fishing have played an important role in the culture and history of the area, information on the specific patterns of consumption by recreational anglers has been previously unavailable. the pilot data presented here will help determine the feasibility of a large-scale survey of sea ... | 2016 | 27891049 |
| co-exposure to sunlight enhances the toxicity of naturally weathered deepwater horizon oil to early lifestage red drum (sciaenops ocellatus) and speckled seatrout (cynoscion nebulosus). | the 2010 deepwater horizon oil spill resulted in the accidental release of millions of barrels of crude oil into the gulf of mexico. photo-induced toxicity following co-exposure to ultraviolet (uv) radiation is 1 mechanism by which polycyclic aromatic hydrocarbons (pahs) from oil spills may exert toxicity. red drum and speckled seatrout are both important fishery resources in the gulf of mexico. they spawn near-shore and produce positively buoyant embryos that hatch into larvae in approximately ... | 2017 | 27868239 |
| vitrification of sperm from marine fishes: effect on motility and membrane integrity. | our goal was to develop a standardized approach for sperm vitrification of marine fishes that can be applied generally in aquatic species. the objectives were to: 1) estimate acute toxicity of cryoprotectants over a range of concentrations; 2) evaluate the properties of vitrification solutions (vs); 3) evaluate different thawing solutions, and 4) evaluate sperm quality after thawing by examination of motility and membrane integrity. sperm were collected from red snapper (lutjanus campechanus), s ... | 2015 | 26074721 |
| sensory development and its relation to habitat change in three species of sciaenids. | visual and mechanosensory development of three sciaenid species was investigated to examine possible correlations between sensory morphology and patterns of habitat use. although the three species have different migration patterns as early larvae, few differences in sensory morphology occurred between species until late in the larval period. atlantic croaker, micropogonias undulatus, were distinguished by enhancements of visual morphology (large eyes, abundant photoreceptors, and best summation ... | 1998 | 9807012 |
| characterization of tilapia (oreochromis niloticus) viperin expression, and inhibition of bacterial growth and modulation of immune-related gene expression by electrotransfer of viperin dna into zebrafish muscle. | viperin is an anti-viral protein, induced by viral infection. in this study, we examined whether over-expression of viperin in fish muscle could inhibit bacterial growth. we first obtained the cdna sequence of tilapia viperin, through rt-pcr-mediated cloning and sequencing. the cdna sequence was similar to those of several fish viperins in genbank, and it was predicted to encode the conserved domain of radical s-adenosylmethionine superfamily proteins. phylogenetic analysis revealed that tilapia ... | 2013 | 23237906 |
| molecular cloning and characterization of high mobility group box1 (ls-hmgb1) from humphead snapper, lutjanus sanguineus. | high mobility group box1 (hmgb1) is a kind of chromatin-associated nonhistone protein important for nucleosome formation, transcriptional regulation and inflammation. however, the reports about hmgb1 of marine fish were still limited. here, we cloned and characterized a hmgb1 gene from humphead snapper, lutjanus sanguineus (ls-hmgb1). the ls-hmgb1 cdna composed of 1199 bp with a 70 bp of 5'-utr, 630 bp open reading frame (orf) and 499 bp 3'-utr, encoded a polypeptide of 210 amino acids (genbank ... | 2014 | 25120217 |
| fishing, fish consumption and advisory awareness among louisiana's recreational fishers. | this paper presents results from the first known population-based survey of recreational fishers in louisiana (n=1774). the ultimate goal of this study was to obtain data in support of the development of regional advisories for a high exposure population with unique seafood consumption patterns. between july and august of 2008, a survey was mailed to a random sample of licensed recreational fishers to characterize local fishing habits, sportfish consumption, and advisory awareness. eighty-eight ... | 2011 | 21851935 |
| [fatty acids in different edible fish species from mexico]. | different biotic and abiotic factors determine the fatty acid (fa) composition of fish tissues and organs. this information is useful for humans due to the fact that fish consumption is associated with health benefits. the aim of the present study was to identify the variation in the concentration of fatty acids, according to different factors, among ten edible marine fish species in mexico, collected from june to december 2009 in the largest fish market in mexico city: euthynnus alletteratus, s ... | 2013 | 24432548 |
| factors affecting toxicity test endpoints in sensitive life stages of native gulf of mexico species. | indigenous species are less commonly used in laboratory aquatic toxicity tests compared with standard test species due to (1) limited availability lack of requisite information necessary for their acclimation and maintenance under laboratory conditions and (2) lack of information on their sensitivity and the reproducibility of toxicity test results. as part of the natural resource damage assessment aquatic toxicity program in response to the deepwater horizon oil incident (2010), sensitive life ... | 2015 | 25563746 |
| oxygen dependence of upper thermal limits in fishes. | temperature-induced limitations on the capacity of the cardiorespiratory system to transport oxygen from the environment to the tissues, manifested as a reduced aerobic scope (maximum minus standard metabolic rate), have been proposed as the principal determinant of the upper thermal limits of fishes and other water-breathing ectotherms. consequently, the upper thermal niche boundaries of these animals are expected to be highly sensitive to aquatic hypoxia and other environmental stressors that ... | 2016 | 27591316 |
| fish otolith asymmetry: morphometry and modeling. | mathematical modeling suggests that relatively large values of otolith mass asymmetry in fishes can alter acoustic functionality and may be responsible for abnormal fish behavior when subjected to weightlessness during parabolic or space flight [d.v. lychakov, y.t. rebane, otolith mass asymmetry in 18 species of fish and pigeon, j. grav. physiol. 11 (3) (2004) 17-34; d.v. lychakov, y.t. rebane, fish otolith mass asymmetry: morphometry and influence on acoustic functionality, hear. res. 201 (2005 ... | 2006 | 16859847 |
| detection of antimicrobial peptides related to piscidin 4 in important aquacultured fish. | epithelial surfaces of fish, such as the gut, skin and gills, comprise a large surface area for possible pathogen invasion. antimicrobial peptides (amps), innate immunity components, play a significant role in protecting fish. piscidins are a family of amps. in this study, we detected the presence of the recently discovered piscidin 4 via bug blot, western blot, elisa and/or immunohistochemistry in striped bass (morone saxatilis), white bass (m. chrysops), european seabass (dicentrarchus labrax) ... | 2010 | 19913049 |
| effects of the synthetic pyrethroid insecticide, permethrin, on two estuarine fish species. | limited toxicity data are available for estuarine and marine species and the widely used pyrethroid insecticide, permethrin. this study determined acute effects of permethrin on survival, lipid peroxidation, acetylcholinesterase activity, and splenocyte proliferation for two fish species found in south carolina estuaries; juvenile red drum (sciaenops ocellatus) and adult mummichog (fundulus heteroclitus). juvenile s. ocellatus were significantly more sensitive than adult f. heteroclitus to perme ... | 2011 | 21756140 |
| cloning and phylogenetic relationship of red drum somatolactin cdna and effects of light on pituitary somatolactin mrna expression. | the nucleotide sequence for red drum somatolactin (sl) cdna was determined and the expression of pituitary sl mrna was examined in red drum kept under various light conditions. a full length of sl cdna (1629 bp) was isolated and characterized from a red drum pituitary cdna library. the sl cdna has an open reading frame of 696 nucleotides which encodes a 24-amino-acid signal peptide and a 207-amino-acid mature peptide. red drum sl shares 58-87% amino acid sequence identity and 56-85% nucleotide s ... | 1999 | 9882545 |
| philometra floridensis sp. n. (nematoda: philometridae) from the ovary of red drum sciaenops ocellatus (osteichthyes: sciaenidae) off the coast of florida, usa. | a new nematode species, philometra floridensis sp. n. (philometridae), is described from male and female specimens found in the ovary of red drum, sciaenops ocellatus (linnaeus) (sciaenidae, perciformes), from the gulf of mexico off treasure island, florida, usa. based on light and scanning electron microscopy examination, the new species differs from most other gonad-infecting philometra spp. in having a smooth gubernaculum with a distinct dorsal tooth on the distal tip. the new species is most ... | 2010 | 19580690 |
| two new species of cardicola (digenea: sanguinicolidae) in drums (sciaenidae) from mississippi and louisiana. | two new species of cardicola (digenea: sanguinicolidae) are described from the heart of drums (perciformes: sciaenidae) in and off mississippi and louisiana. cardicola palmeri n. sp. infects the black drum, pogonias cromis, and, in mature individuals, is distinguished from its congeners by the combination of a body 1.9-2.9 times longer than wide, an esophagus 44-52% of the body length, posterior ceca 1.0-1.8 times longer than the anterior ones, a gland encircling the seminal vesicle that is 30-3 ... | 2004 | 15040679 |
| the effect of structural complexity, prey density, and "predator-free space" on prey survivorship at created oyster reef mesocosms. | interactions between predators and their prey are influenced by the habitat they occupy. using created oyster (crassostrea virginica) reef mesocosms, we conducted a series of laboratory experiments that created structure and manipulated complexity as well as prey density and "predator-free space" to examine the relationship between structural complexity and prey survivorship. specifically, volume and spatial arrangement of oysters as well as prey density were manipulated, and the survivorship of ... | 2011 | 22145037 |
| the high mobility group box 1 protein of sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation. | high mobility group box 1 protein (hmgb1) is a chromatin-associated nonhistone protein that is involved in nucleosome formation and transcriptional regulation. in addition, hmgb1 is also known as an extracellular cytokine that triggers inflammation and immune responses. hmgb1-like sequences have been identified in a number of fish species, however, the function of piscine hmgb1 remains uninvestigated. in this study, we reported the identification and analysis of sohmgb1, an hmgb1 homologue from ... | 2011 | 21527276 |
| perfluorinated compounds in seafood from coastal areas in china. | diet is an important source of perfluorinated compound (pfc) exposure and seafood is an important diet component for coastal populations. therefore, it is necessary to monitor the concentrations of pfcs in seafood. in this study, we measured thirteen pfcs in 47 fatty fish and 45 shellfish samples collected from six coastal provinces in china (liaoning, shandong, jiangsu, zhejiang, fujian, and guangdong), using an ultra-performance liquid chromatography and tandem mass spectrometry (uplc-ms/ms). ... | 2011 | 21550116 |
| fish tissue quality in the lower mississippi river and health risks from fish consumption. | between 1990 and 1994, samples of three shellfish species (i.e. blue crab, callinectes sapidus;crayfish, procambarus acutis; and river shrimp, macrobrachium ohionii) and 16 fish species and were collected at six sites along the lower mississippi river by the louisiana department of environmental quality, office of water resources in coordination with the us environmental protection agency. the fish species included: bigmouth buffalo (ictiobus cyanellus); blue catfish (ictalurus furcatus); carp ( ... | 2003 | 12526903 |
| wood preservative leachates from docks in an estuarine environment. | environmental concentrations and biological effects of certain metals and organic compounds found in wood preservatives were examined. the study focused on leachates from private residential docks in south carolina tidal creeks. copper, chromium, arsenic, and polynuclear aromatic hydrocarbons (pahs) were measured in composite samples of surficial sediments and naturally occurring oyster populations (crassostrea virginica) from creeks with high densities of docks, and from nearby reference creeks ... | 1996 | 8687987 |