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eubacterium minutum is an earlier synonym of eubacterium tardum and has priority.the recently proposed species eubacterium minutum and eubacterium tardum appeared to be similar from their published descriptions. the aim of this study was to perform phenotypic and genetic analyses of strains of both species to clarify their taxonomic position. the type strains of e. minutum and e. tardum exhibited identical biochemical and protein profiles and their 16s rrna gene sequences displayed 99.9% similarity. the g + c content of the dna of both strains was estimated at 45 mol%. it is ...199910555379
subgingival microbiome in patients with healthy and ailing dental implants.dental implants are commonly used to replace missing teeth. however, the dysbiotic polymicrobial communities of peri-implant sites are responsible for peri-implant diseases, such as peri-implant mucositis and peri-implantitis. in this study, we analyzed the microbial characteristics of oral plaque from peri-implant pockets or sulci of healthy implants (n = 10), peri-implant mucositis (n = 8) and peri-implantitis (n = 6) sites using pyrosequencing of the 16s rrna gene. an increase in microbial di ...201526077225
phylogeny of oral asaccharolytic eubacterium species determined by 16s ribosomal dna sequence comparison and proposal of eubacterium infirmum sp. nov. and eubacterium tardum sp. nov.16s rrna gene sequences of eubacterium brachy, eubacterium nodatum, eubacterium saphenum, eubacterium timidum, and two previously unnamed taxa were determined. the results of a phylogenetic analysis indicated that all of the strains sequenced belonged to a deep branch of the low-g+c-content gram-positive group. the levels of 16s ribosomal dna sequence similarity between species were low, suggesting that a number of genera may be represented in this group. the representatives of the two unnamed t ...19968863423
eubacterium minutum sp. nov., isolated from human periodontal pockets.we describe eubacterium minutum sp. nov., which was isolated from human periodontal pockets. this new species was established on the basis of dna-dna hybridization data. the guanine-plus-cytosine content of its dna is 38 to 40 mol%. the results of differential biochemical and enzymatic tests are described. the type strain of this species is strain m-6.19968573510
cryptobacterium curtum gen. nov., sp. nov., a new genus of gram-positive anaerobic rod isolated from human oral cavities.novel eubacterium-like isolates, strains 12-3t and kv43-b, which were isolated from the periodontal pocket of an adult patient with periodontal disease and necrotic dental pulp, respectively, were studied taxonomically and phylogenetically. the morphological and differential biochemical characteristics of these organisms are also described in this paper. these organisms were gram-positive, anaerobic, non-spore-forming, rod-shaped bacteria that were inert in most of the conventional biochemical t ...199910425779
restriction fragment-length polymorphism analysis of 16s rdna from oral asaccharolytic eubacterium species amplified by polymerase chain reaction.restriction fragment-length polymorphism (rflp) analysis of 16s rdna amplified by polymerase chain reaction was used to generate restriction profiles of the type strains of oral asaccharolytic eubacterium species, that is, eubacterium brachy, eubacterium exiguum, eubacterium lentum, eubacterium minutum, eubacterium nodatum, eubacterium saphenum, eubacterium timidum and 33 asaccharolytic eubacterium strains isolated from oral sites. the 16s rrna gene sequences from isolated genomic dna samples we ...19989573818
degradation of arginine and other amino acids by butyrate-producing asaccharolytic anaerobic gram-positive rods in periodontal pockets.the use of 20 amino acids by butyrate-producing asaccharolytic anaerobic gram-positive rods (aagprs) in periodontal pockets, i.e. eubacterium minutum, filifactor alocis, e. infirmum, e. sulci and e. saphenum, was studied. e. minutum used only arginine and lysine, and produced substantial amounts of butyrate and ammonia as the main metabolic products from arginine, and acetate, butyrate and ammonia from lysine. fi. alocis used arginine alone and produced butyrate and ammonia. e. infirmum, e. sulc ...200312749914
taxonomic characterization of mogibacterium diversum sp. nov. and mogibacterium neglectum sp. nov., isolated from human oral cavities.novel isolates, strains hm-7, hm-6, hh-31, p9a-ht and ujb13-d, which were isolated from tongue plaque and necrotic dental pulp, were studied taxonomically and phylogenetically. these organisms were anaerobic, non-spore-forming, gram-positive, rod-shaped bacteria that were inert in most of the conventional biochemical tests and phenotypically resemble mogibacterium species or asaccharolytic eubacterium species. the g+c contents of the dnas from the novel isolates ranged from 41 to 42 mol %. dna-d ...200211837293
description of mogibacterium pumilum gen. nov., sp. nov. and mogibacterium vescum gen. nov., sp. nov., and reclassification of eubacterium timidum (holdeman et al. 1980) as mogibacterium timidum gen. nov., comb. nov.a new genus, mogibacterium, is proposed for anaerobic, non-spore-forming, gram-positive, rod-shaped bacteria which have been isolated from the periodontal pockets of adult human patients with periodontal disease and infected root canals. the novel isolates, strains d2-18t, ba11a-f and d5-2t, were inert in most of the conventional biochemical tests and phenotypically resemble asaccharolytic eubacterium species. the protein profiles of whole cells on sds-page gels and western immunoblotting reacti ...200010758875
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