| identification and characterization of a novel tospovirus species using a new rt-pcr approach. | a novel tospovirus serologically distinct from all established tospovirus species was found in thailand in physalis minima l. the s rna of this virus was cloned by a new rt-pcr approach revealing a nucleotide sequence of 3257 nucleotides. the ambisense rna segment encoded a nonstructural protein (nss) of 469 amino acids, with a predicted mr of 53.2 kda, and a nucleoprotein (n) of 279 amino acids and a mr of 31.0 kda, so far the largest n protein known for any tospovirus species. n protein sequen ... | 2001 | 11315637 |
| purification and serological analyses of tospoviral nucleocapsid proteins expressed by zucchini yellow mosaic virus vector in squash. | a plant viral vector engineered from an in vivo infectious clone of zucchini yellow mosaic virus (zymv) was used to express the nucleocapsid proteins (nps) of tospoviruses in planta. the open reading frames (orfs) of nps of different serogroups of tospoviruses, including tomato spotted wilt virus, impatiens necrotic spot virus, watermelon silver mottle virus, peanut bud necrosis virus, and watermelon bud necrosis virus (wbnv), were in frame inserted in between the p1 and hc-pro genes of the zymv ... | 2005 | 15992936 |
| immunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of groundnut bud necrosis virus. | in vitro gene expression strategy was used for the production of polyclonal antiserum to the nucleocapsid protein (np) of groundnut bud necrosis virus (gbnv). the gbnv np gene from cowpea isolate was cloned into 6x his-tagged ua cloning vector and expressed in escherichia coli [m15] cells. the fusion protein was detected in insoluble fraction and was purified by using ni-nta agarose resin. the purified 6x his-fusion protein ( approximately 32 kda) was used for immunisation to produce a high titr ... | 2005 | 16095728 |
| serological relationship between melon yellow spot virus and watermelon silver mottle virus and differential detection of the two viruses in cucurbits. | melon yellow spot virus (mysv), a tentative member of the genus tospovirus, is considered a distinct serotype due to the lack of a serological relationship with other tospoviruses in its nucleocapsid protein (np). recently, a virus isolate collected from diseased watermelon in central taiwan (mysv-tw) was found to react with a rabbit antiserum (ras) prepared against the np of watermelon silver mottle virus (wsmov), and a monoclonal antibody (mab) prepared against the common epitope of the nss pr ... | 2010 | 20480192 |
| genome organisation and sequence comparison suggest intraspecies incongruence in m rna of watermelon bud necrosis virus. | watermelon bud necrosis virus (wbnv), a member of the genus tospovirus, family bunyaviridae is an important viral pathogen in watermelon cultivation in india. the complete genome sequence properties of wbnv are not available. in the present study, the complete m rna sequence and the genome organisation of a wbnv isolate infecting watermelon in delhi (wbnv-wdel) were determined. the m rna was 4,794 nucleotides (nt) long and potentially coded for a movement protein (nsm) of 34.22 kda (307 amino ac ... | 2010 | 20480193 |
| complete genomic sequence of watermelon bud necrosis virus. | | 2010 | 21184244 |
| importance and genetic diversity of vegetable-infecting tospoviruses in india. | a survey for peanut bud necrosis virus (pbnv), watermelon bud necrosis virus (wbnv), capsicum chlorosis virus (cacv), and iris yellow spot virus (iysv) was conducted between 2002 and 2009 in the major vegetable-growing areas in india. pbnv was documented widely in tomato and chili peppers in 14 states representing southern, north-western, north-eastern, and central regions and wbnv was predominantly detected in watermelons and cucurbits in all except north-eastern regions. in addition, the expan ... | 2011 | 21299415 |
| engineered single-chain variable fragment antibody for immunodiagnosis of groundnut bud necrosis virus infection. | few studies have been done on engineered antibodies for diagnosis of tospovirus infections. the present study was undertaken to develop a single-chain variable fragment (scfv) for specific diagnosis of infection by groundnut bud necrosis virus (gbnv), the most prevalent serogroup iv tospovirus in india. heavy chain (372 nucleotide [nt]) and light chain (363 nt) variable region clones obtained from a hybridoma were used to make an scfv construct that expressed a ~29-kda protein in e. coli. the sc ... | 2015 | 25698103 |
| development of interspecific solanum lycopersicum and screening for tospovirus resistance. | tospovirus has emerged as a serious viral pathogen for several crops including tomato. the tomato production is being severely affected worldwide by tospovirus. some reports have been published about the association of plant virus and development of human disease either by direct or indirect consumption. resistance to this virus has been identified as good source in wild tomato species (lycopersicum peruvianum). but the introgression of resistance genes into cultivated tomato lines and the devel ... | 2015 | 26587001 |