| the nucleotide sequence of a chinese isolate of wheat yellow mosaic virus and its comparison with a japanese isolate. brief report. | the nucleotide sequences of wheat yellow mosaic virus isolated in china were determined and compared with a japanese isolate of the same virus. results showed that the viral genome had 7629 nucleotides for rna1 and 3639 nucleotides for rna2, which shared 97. 1% and 94.6% of identities to the rnas of japanese isolate. the single open reading frames in rna1 and rna2 encoded polyproteins with 2407 amino acids and 903 amino acids respectively, from which ten proteins may be produced by autolytic cle ... | 1999 | 10603173 |
| a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family. | a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ... | 2001 | 11402861 |
| [development of a system for polymyxa graminis infection and transmission of wheat yellow mosaic virus]. | in growth chambers, a system was developed for infection of polymyxa graminis to wheat(triticum aestivum l.), which is an obligate parasite. by the system, the fungus could be propagated in large quantity rapidly and the life cycle of p. graminis in wheat was observed in a period of 13 to 15 days. the methods for separating p. graminis as single cystosori from cultured wheat roots or dried roots were simplified. by tests for the factors affecting on p. graminis infection, including inocula types ... | 2000 | 12548954 |
| a one step real-time rt-pcr assay for the quantitation of wheat yellow mosaic virus (wymv). | wheat yellow mosaic virus (wymv) is an important pathogen in china and other countries. it is the member of the genus bymovirus and transmitted primarily by polymyxa graminis. the incidence of wheat infections in endemic areas has risen in recent years. prompt and dependable identification of wymv is a critical component of response to suspect cases. | 2013 | 23725024 |
| selection pressure and founder effects constrain genetic variation in differentiated populations of soilborne bymovirus wheat yellow mosaic virus (potyviridae) in china. | to study the population genetic structure and forces driving the evolution of wheat yellow mosaic virus (wymv), the nucleotide sequences encoding the coat protein (cp) (297 sequences) or the genome-linked virion protein (vpg) (87 sequences) were determined from wheat plants growing at 11 different locations distributed in five provinces in china. there were close phylogenetic relationships between all sequences but clustering on the phylogenetic trees was congruent with their provenance, suggest ... | 2013 | 23550972 |
| nucleo-cytoplasmic shuttling of vpg encoded by wheat yellow mosaic virus requires association with the coat protein. | vpg (virus protein, genome-linked) is a multifunctional protein that plays important roles in viral multiplication in the cytoplasm. however, a number of vpgs encoded by plant viruses target the nucleus and this appears to be biologically significant. these vpgs may therefore be translocated between nuclear and cytoplasmic compartments during virus infection, but such nucleo-cytoplasmic transport has not been demonstrated. we report that vpg encoded by wheat yellow mosaic virus (wymv, genus bymo ... | 2013 | 24045111 |
| the p2 of wheat yellow mosaic virus rearranges the endoplasmic reticulum and recruits other viral proteins into replication-associated inclusion bodies. | viruses commonly modify host endomembranes to facilitate biological processes in the viral life cycle. infection by viruses belonging to the genus bymovirus (family potyviridae) has long been known to induce the formation of large membranous inclusion bodies in host cells, but their assembly and biological roles are still unclear. immunoelectron microscopy of cells infected with the bymovirus wheat yellow mosaic virus (wymv) showed that p1, p2 and p3 are the major viral protein constituents of t ... | 2014 | 24304930 |
| biological and genetic diversity of wheat yellow mosaic virus (genus bymovirus). | the biological and genetic diversity of wheat yellow mosaic virus (wymv) isolates in japan was characterized. on the basis of wheat cultivar reactions, 14 wymv isolates from various places were classified into pathotypes i, ii, or iii. these were distributed in central, northern, and southern areas of japan, respectively. wymv isolates comprised three genotypes (a, a' and b) based on amino acid differences in rna1 and two genotypes (a and b) based on amino acid differences in rna2. a correlation ... | 2014 | 24512116 |
| resistance to wheat yellow mosaic virus in madsen wheat is controlled by two major complementary qtls. | wheat yellow mosaic virus resistance of madsen is governed by two complementary qtls, qym1 and qym2 , located on chromosome arms 2dl and 3bs. wheat yellow mosaic, caused by wheat yellow mosaic virus (wymv), is one of the most serious wheat diseases in east asia. in this study, recombinant inbred lines (rils, f9) from a cross between cultivars madsen (resistant) and hokushin (susceptible) grown in a wymv-infected nursery field were tested for the presence of wymv in leaves by enzyme-linked immuno ... | 2015 | 25957645 |
| investigating pollen and gene flow of wymv-resistant transgenic wheat n12-1 using a dwarf male-sterile line as the pollen receptor. | pollen-mediated gene flow (pmgf) is the main mode of transgene flow in flowering plants. the study of pollen and gene flow of transgenic wheat can help to establish the corresponding strategy for preventing transgene escape and contamination between compatible genotypes in wheat. to investigate the pollen dispersal and gene flow frequency in various directions and distances around the pollen source and detect the association between frequency of transgene flow and pollen density from transgenic ... | 2016 | 26975052 |
| induction of 4vs chromosome recombinants using the cs ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from haynaldia villosa. | the wheat spindle streak mosaic virus (wssmv) or wheat yellow mosaic virus (wymv) resistance gene, wss1, from haynaldia villosa, was previously mapped to the chromosome arm 4vs by the development of 4v (4d) substitution and t4dl·4vs translocation lines. for better utilization and more accurate mapping of the wss1, in this research, the cs ph1b mutant was used to induce new translocations with shortened 4vs chromosome fragments. thirty-five homozygous translocations with different alien fragment ... | 2013 | 23989649 |
| durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene. | wheat yellow mosaic virus (wymv) has spread rapidly and causes serious yield losses in the major wheat-growing areas in china. because it is vectored by the fungus-like organism polymyxa graminis that survives for long periods in soil, it is difficult to eliminate by conventional crop management or fungicides. there is also only limited resistance in commercial cultivars. in this research, fourteen independent transgenic events were obtained by co-transformation with the antisense nib8 gene (the ... | 2014 | 24373454 |
| impact of transgenic wheat with wheat yellow mosaic virus resistance on microbial community diversity and enzyme activity in rhizosphere soil. | the transgenic wheat line n12-1 containing the wymv-nib8 gene was obtained previously through particle bombardment, and it can effectively control the wheat yellow mosaic virus (wymv) disease transmitted by polymyxa graminis at turngreen stage. due to insertion of an exogenous gene, the transcriptome of wheat may be altered and affect root exudates. thus, it is important to investigate the potential environmental risk of transgenic wheat before commercial release because of potential undesirable ... | 2014 | 24897124 |
| differential detection of wheat yellow mosaic virus, japanese soil-borne wheat mosaic virus and chinese wheat mosaic virus by reverse transcription loop-mediated isothermal amplification reaction. | a differential detection method for three wheat viruses: wheat yellow mosaic virus (wymv), japanese soil-borne mosaic virus (jsbwmv) and chinese wheat mosaic virus (cwmv) using reverse transcription loop-mediated isothermal amplification (rt-lamp) reaction was developed. all three primer sets, which were designed from the genome sequences of wymv, jsbwmv and cwmv respectively, worked most efficiently at 65 °c and could detect each virus rna within 10 min by fluorescence monitoring using an isoth ... | 2013 | 23523736 |
| association of vpg and eif4e in the host tropism at the cellular level of barley yellow mosaic virus and wheat yellow mosaic virus in the genus bymovirus. | barley yellow mosaic virus (baymv) and wheat yellow mosaic virus (wymv) are separate species in the genus bymovirus with bipartite plus-sense rna genomes. in fields, baymv infects only barley and wymv infects only wheat. here, we studied the replicative capability of the two viruses in barley and wheat mesophyll protoplasts. baymv replicated in both barley and wheat protoplasts, but wymv replicated only in wheat protoplasts. the expression of wheat translation initiation factor 4e (eif4e), a com ... | 2015 | 25543966 |
| development of a multiplex polymerase chain reaction for simultaneous detection of wheat viruses and a phytoplasma in china. | barley yellow dwarf viruses (bydvs, mainly consisting of three strains, gav, gpv and pav, in china), barley stripe mosaic virus (bsmv), wheat yellow mosaic virus (wymv), wheat dwarf virus (wdv) and wheat blue dwarf phytoplasma (wbd) constitute a group of major wheat pathogens that have caused huge yield losses but are scarcely distinguished by their phenotype alone. for the simultaneous detection and discrimination of these seven pathogens in wheat, a multiplex polymerase chain reaction (m-pcr) ... | 2012 | 22456911 |
| Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification. | ABSTRACT: For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Using Primer Explorer software, four sets of primers were designed and RT-LAMP assay reaction conditions were optimized. The RT-LAMP was performed at different times by four primer sets. Agarose gel analysis showed that WYMV could be detected after 30 min with the primer set III and after 45 min with the other three primer sets, both und ... | 2011 | 22185375 |
| soil-borne viruses affecting cereals: known for long but still a threat. | a number of furo- and bymoviruses, which are naturally transmitted by the ubiquitous soil-borne protozoon polymyxa graminis are of great economic importance for autumn-sown wheat, barley, rye, triticale, and oats. the two barley-affecting bymoviruses, barley yellow mosaic virus and barley mild mosaic virus, are widespread in europe and east asia. production of wheat, rye, and triticale is threatened by three related furoviruses and two bymoviruses. soil-borne wheat mosaic virus mainly occurs in ... | 2009 | 19159654 |
| analysis of nucleotide sequence of wheat yellow mosaic virus genomic rnas. | wheat yellow mosaic virus (wymv) isolate hc was used for viral cdna synthesis and sequencing. the results show that the viral rnal is 7 629 nucleotides encoding a polyprotein with 2 407 amino acids, from which seven putative proteins may be produced by an autolytic cleavage processing besides the viral coat protein. the rna2 is 3 639 nucleotides and codes for a polyprotein of 903 amino acids, which may contain two putative non-structural proteins. although wymv shares a similarity in genetic org ... | 1999 | 18726520 |