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culturable bacteria in hydroponic cultures of moss racomitrium japonicum and their potential as biofertilizers for moss production.the use of racomitrium japonicum, a drought resistant bryophyte used for roof-greening, is gradually increasing. however, its utilization is hampered by slow growth rate. here we isolated culturable bacteria from hydroponic cultivation samples to identify isolates that could promote moss growth. most of the isolates belonged to pseudomonas, rhodococcus, and duganella species. the isolates were biochemically characterized according to their type of interaction with plants, i.e., production of aux ...201121498111
violacein-producing collimonas sp. from the sea surface microlayer of costal waters in trøndelag, norway.a new strain belonging to the genus collimonas was isolated from the sea surface microlayer off the coast of trøndelag, norway. the bacterium, designated collimonas ct, produced an antibacterial compound active against micrococcus luteus. subsequent studies using lc-ms identified this antibacterial compound as violacein, known to be produced by several marine-derived bacteria. fragments of the violacein biosynthesis genes vioa and viob were amplified by pcr from the collimonas ct genome and sequ ...200920098599
reconstruction of the violacein biosynthetic pathway from duganella sp. b2 in different heterologous hosts.violacein is a bacteria-originated indolocarbazole pigment with potential applications due to its various bioactivities such as anti-tumor, antiviral, and antifungal activities. however, stable mass production of this pigment is difficult due to its low productivities and the instability of wild-type violacein-producing strains. in order to establish a stable and efficient production system for violacein, the violacein synthesis pathway from a new species of duganella sp. b2 was reconstructed in ...201020012278
composition of bacterial communities associated with natural and laboratory populations of asobara tabida infected with wolbachia.asobara tabida wasps are fly endoparasitoids that naturally harbor three wolbachia strains, which induce cytoplasmic incompatibility and control oogenesis. to investigate whether other bacteria play a role in wasp biology, we surveyed the bacterial communities of wild a. tabida populations originating from different regions of france and of laboratory colonies using pcr-denaturing gradient gel electrophoresis and culture methods. proteobacteria and firmicutes were found to be the main phyla repr ...200919376923
undibacterium pigrum gen. nov., sp. nov., isolated from drinking water.two gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacteria (strains ccug 49009(t) and ccug 49012), both isolated from drinking water, were characterized. on the basis of chemotaxonomic data [major ubiquinone, q-8; predominant polyamines, putrescine and 2-hydroxyputrescine; major polar lipids, phosphatidylethanolamine, moderate amounts of diphosphatidylglycerol and phosphatidylglycerol and minor amounts of three aminolipids and phosphatidylserine; major fatty acids, c ...200717625185
purple-pigmented violacein-producing duganella spp. inhabit the rhizosphere of wild and cultivated olives in southern spain.bacteria have evolved mechanisms that allow them to grow and survive in highly competitive environments like soil and the rhizosphere. using classical microbiological, physiological, and genetic analyses, we isolated and identified for the first time duganella spp. associated with the rhizosphere of woody plants in mediterranean environments that are able to produce violacein, a blue-purple secondary metabolite of considerable biotechnological interest. based on physiological and biochemical cha ...201121424823
duganella phyllosphaerae sp. nov., isolated from the leaf surface of trifolium repens and proposal to reclassify duganella violaceinigra into a novel genus as pseudoduganella violceinigra gen. nov., comb. nov.a bright yellow pigmented bacterium was isolated from the leaf surface of trifolium repens in germany. comparative analysis of 16s rrna gene sequences showed that this bacterium is most closely related to duganella zoogloeoides iam 12670(t), with a similarity of 99.3%, but revealed only a moderate similarity (96.8%) to the second duganella species, duganella violaceinigra yim 31327(t). strain t54(t) is clearly different from d. zoogloeoides iam 12670(t) in that dna-dna hybridization revealed a s ...201222169565
characterization of psychrotolerant heterotrophic bacteria from finnish lapland.a total of 331 aerobic heterotrophic bacterial strains were isolated from various ecosystems of finnish lapland (68-69 degrees n) including forest soil, arctic alpine-tundra soil, stream water, lake and mire sediments, lichen and snow algae. whole cell fatty acid and 16s rrna gene sequence analysis and microscopy indicated that the isolates were dominated by gram-negative bacteria, while only 20 gram-positive strains were isolated. based on 16s rrna gene sequences the isolates were members of al ...200616564959
alpha- and beta-proteobacteria control the consumption and release of amino acids on lake snow aggregates.we analyzed the composition of aggregate (lake snow)-associated bacterial communities in lake constance from 1994 until 1996 between a depth of 25 m and the sediment surface at 110 m by fluorescent in situ hybridization with rrna-targeted oligonucleotide probes of various specificity. in addition, we experimentally examined the turnover of dissolved amino acids and carbohydrates together with the microbial colonization of aggregates formed in rolling tanks in the lab. generally, between 40 and m ...200111157226
use of plate-wash samples to monitor the fates of culturable bacteria in mercury- and trichloroethylene-contaminated soils.with the ultimate aim of developing bioremediation technology that use the optimum bacterial community for each pollutant, we performed polymerase chain reaction (pcr)-denaturing gradient gel electrophoresis (dgge) and phylogenetic analysis and identified communities of culturable bacteria in hgcl(2)- and trichloroethylene (tce)-contaminated soil microcosms. pcr-dgge band patterns were similar at 0 and 1 ppm hgcl(2), but changes in specific bands occurred at 10 ppm hgcl(2). band patterns appeari ...200717940764
janthinobacterium agaricidamnosum sp. nov., a soft rot pathogen of agaricus bisporus.a novel bacterium has been found that causes a soft rot disease of agaricus bisporus, the cultivated mushroom. it has been characterized using nutritional, physiological, chemical and molecular techniques. based on these data, it was shown to have many characteristics in common with members of the genus janthinobacterium. despite similarities to the only described species within this genus, janthinobacterium lividum, there were a number of differences between the mushroom pathogen isolated and t ...199910555339
massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions.a fastidious, slowly growing, strictly aerobic, gram-negative bacterium was isolated from a culture of blood from a 25-year-old man with common variable immunodeficiency. the man had been admitted to hospital with febrile progressive cerebellar ataxia. the use of standard phenotypic schemes did not lead to identification, but sequence analysis demonstrated that the 16s rrna gene of the isolate was most similar to those of the environmental bacteria duganella zoogloeoides (formerly zoogloea ramig ...19989738031
duganella violaceinigra sp. nov., a novel mesophilic bacterium isolated from forest soil.a mesophilic bacterium, designated strain yim 31327t, was isolated from a forest soil sample collected from yunnan province, china, and was then investigated using a polyphasic approach. the strain grew optimally at 28-30 degrees c and ph 7.2. the cells were gram-negative, short, rod-shaped, motile and non-spore-forming with flagella. the major ubiquinone was q-8 and the cellular fatty acids were c(16 : 0) and c(12 : 0). the dna g + c content of strain yim 31327t was 62.8 mol%. phylogenetic anal ...200415388748
proposal to reclassify zoogloea ramigera iam 12670 (p. r. dugan 115) as duganella zoogloeoides gen. nov., sp. nov.the taxonomic position of a misclassified strain, zoogloea remigera iam 12670t (= atcc 25925t = p. r. dugan 115t), was reevaluated. a phylogenetic analysis based on 16s ribosomal rdna sequences revealed that this organism was located in the beta subclass of the class proteobacteria with members of the genus telluria as its closest relatives. on the basis of phenotypic and phylogenetic information, we propose that this organism should be reclassified in a new taxon with the name duganella zoogloe ...19979336937
engineering root exudation of lotus toward the production of two novel carbon compounds leads to the selection of distinct microbial populations in the rhizosphere.the culture of opine-producing transgenic lotus plants induces the increase in the rhizosphere of bacterial communities that are able to utilize these molecules as sole carbon source. we used transgenic lotus plants producing two opines, namely mannopine and nopaline, to characterize the microbial communities directly influenced by the modification of root exudation. we showed that opine-utilizers represent a large community in the rhizosphere of opine-producing transgenic lotus. this community ...200415259274
phylogenetic comparisons of a coastal bacterioplankton community with its counterparts in open ocean and freshwater systems.in order to extend previous comparisons between coastal marine bacterioplankton communities and their open ocean and freshwater counterparts, here we summarize and provide new data on a clone library of 105 ssu rrna genes recovered from seawater collected over the western continental shelf of the usa in the pacific ocean. comparisons to previously published data revealed that this coastal bacterioplankton clone library was dominated by ssu rrna gene phylotypes originally described from surface w ...200011098073
specific ribosomal dna sequences from diverse environmental settings correlate with experimental contaminants.phylogenetic analysis of 16s ribosomal dna (rdna) clones obtained by pcr from uncultured bacteria inhabiting a wide range of environments has increased our knowledge of bacterial diversity. one possible problem in the assessment of bacterial diversity based on sequence information is that pcr is exquisitely sensitive to contaminating 16s rdna. this raises the possibility that some putative environmental rrna sequences in fact correspond to contaminant sequences. to document potential contaminant ...19989687486
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