| taxonomic study of bacteria isolated from natural mineral waters: proposal of pseudomonas jessenii sp. nov. and pseudomonas mandelii sp. nov. | the taxonomic position of 23 strains isolated from mineral waters and previously grouped in the authentic pseudomonads on the basis of a phenotypic analysis (cluster ix, subclusters xiiia and xiiic of verhille, s., elomari, m., coroler, l., izard, d., leclerc, h. (syst. appl. microbiol, 20, 137-149, 1997) has been genotypically further studied in the present work. on the basis of hybridization results, these strains were gathered into two new genomic groups for which we propose the names of pseu ... | 1999 | 10188278 |
| siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads. | a total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. as a general rule, strains within a well-circumscribed taxonomic group, namely the species pseudomonas brassicacearum, pseudomonas fuscovaginae, pseudo ... | 2002 | 12039729 |
| nitric oxide reductase-targeted real-time pcr quantification of denitrifier populations in soil. | the quantification of denitrifying bacteria is a component in the further understanding of denitrification processes in the environment. real-time pcr primers were designed to target two segments of the denitrifier population (cnorb(p) [pseudomonas mandelii and closely related strains] and cnorb(b) [bosea, bradyrhizobium, and ensifer spp.]) in agricultural soils based on functional cnorb (nitric oxide reductase) gene sequences. total population numbers were measured using 16s rrna gene real-time ... | 2007 | 17449686 |
| influence of temperature on microbial colonisation of clayey schist as a support media of a submerged filter for groundwater denitrification. | the effect of temperature on biofilm formation and denitrification activity was evaluated. assays were made in a lab-scale submerged filter for the denitrification of polluted groundwater, with and without a previous inoculation. the inoculation was carried out with a selected strain of pseudomonas mandelii. different temperatures were tested: 5, 10, 20 and 30 degrees c. biofilm observations were made, and monitoring of the denitrification capacity of the system was maintained during the experim ... | 2007 | 17546983 |
| taxonomic heterogeneity, as shown by siderotyping, of strains primarily identified as pseudomonas putida. | one hundred and forty-four fluorescent pseudomonad strains isolated from various environments (soil, water, plant rhizosphere, hospital) and received as pseudomonas putida (83 strains), p. putida biovar a (49 strains), p. putida biovar b (10 strains) and p. putida biovar c (2 strains), were analysed by the pyoverdine-isoelectrofocusing and pyoverdine-mediated iron uptake methods of siderotyping. both methods demonstrated a great diversity among these strains, which could be subdivided into 35 si ... | 2007 | 17978216 |
| changes in bacterial denitrifier community abundance over time in an agricultural field and their relationship with denitrification activity. | this study measured total bacterial and denitrifier community abundances over time in an agricultural soil cropped to potatoes (solanum tuberosum l.) by using quantitative pcr. samples were collected on 10 dates from spring to autumn and from three spatial locations: in the potato "hill" between plants (h), close to the plant (h(p)), and in the "furrow" (f). the denitrification rates, n(2)o emissions, and environmental parameters were also measured. changes in denitrifier abundance over time and ... | 2008 | 18689522 |
| nitric oxide reductase gene expression and nitrous oxide production in nitrate-grown pseudomonas mandelii. | pure cultures of pseudomonas mandelii were incubated with or without nitrate, which acts as a substrate and an electron acceptor for denitrification. nitric oxide reductase (cnorb) gene expression was measured using a quantitative reverse transcription-pcr, and nitrous oxide emissions were measured by gas chromatography. p. mandelii cells in either the presence or absence of nitrate demonstrated an increase in cnorb gene expression during the first 3 h of growth. the level of expression of cnorb ... | 2008 | 18820058 |
| effect of ph and temperature on denitrification gene expression and activity in pseudomonas mandelii. | pseudomonas mandelii liquid cultures were studied to determine the effect of ph and temperature on denitrification gene expression, which was quantified by quantitative reverse transcription-pcr. denitrification was measured by the accumulation of nitrous oxide (n(2)o) in the headspace in the presence of acetylene. levels of gene expression of nirs and cnorb at ph 5 were 539-fold and 6,190-fold lower, respectively, than the levels of gene expression for cells grown at ph 6, 7, and 8 between 4 h ... | 2009 | 19376915 |
| effect of nitrate and acetylene on nirs, cnorb, and nosz expression and denitrification activity in pseudomonas mandelii. | nitrate acts as an electron acceptor in the denitrification process. the effect of nitrate in the range of 0 to 1,000 mg/liter on pseudomonas mandelii nirs, cnorb, and nosz gene expression was studied, using quantitative reverse transcription-quantitative pcr. denitrification activity was measured by using the acetylene blockage method and gas chromatography. the effect of acetylene on gene expression was assessed by comparing denitrification gene expression in p. mandelii culture grown in the p ... | 2009 | 19525277 |
| changes in denitrifier abundance, denitrification gene mrna levels, nitrous oxide emissions, and denitrification in anoxic soil microcosms amended with glucose and plant residues. | in agricultural cropping systems, crop residues are sources of organic carbon (c), an important factor influencing denitrification. the effects of red clover, soybean, and barley plant residues and of glucose on denitrifier abundance, denitrification gene mrna levels, nitrous oxide (n(2)o) emissions, and denitrification rates were quantified in anoxic soil microcosms for 72 h. nosz gene abundances and mrna levels significantly increased in response to all organic carbon treatments over time. in ... | 2010 | 20154105 |
| effect of nitrate and glucose addition on denitrification and nitric oxide reductase (cnorb) gene abundance and mrna levels in pseudomonas mandelii inoculated into anoxic soil. | the effect of glucose addition (0 and 500 µg c g(-1) soil) and nitrate (no(3)) addition (0, 10, 50 and 500 µg no(3)-n g(-1) soil) on nitric oxide reductase (cnorb) gene abundance and mrna levels, and cumulative denitrification were quantified over 48 h in anoxic soils inoculated with pseudomonas mandelii. addition of glucose-c significantly increased cnorb(p) (p. mandelii and related species) mrna levels and abundance compared with soil with no glucose added, averaged over time and no(3) additio ... | 2011 | 21431983 |
| a novel eurythermic and thermostale lipase lipm from pseudomonas moraviensis m9 and its application in the partial hydrolysis of algal oil. | lipases are regularly used in biotechnology to catalyse the hydrolysis of triglycerides and the synthesis of esters. microbial lipases in particular have been widely used in a variety of industrial applications. however, the current commercial microbial lipases cannot meet industrial demand due to rapid inactivation under harsh conditions. therefore, in order to identify more stable enzymes, we isolated novel eurythermic and thermostable lipase(s) from pseudomonas moraviensis m9. | 2015 | 26463643 |
| genome sequence of cold-adapted pseudomonas mandelii strain jr-1. | pseudomonas mandelii is a cold-adapted bacterium that can grow at 4°c but not at 37°c. here we report the draft genome sequence of p. mandelii strain jr-1. | 2012 | 22628497 |
| antibiotic resistance of gram-negative benthic bacteria isolated from the sediments of kardzhali dam (bulgaria). | the aim of the present study was to carry out a preliminary assessment for the occurrence of bacterial strains resistant to frequently used antibiotics in the sediments beneath the sturgeon cage farm in kardzhali dam (bulgaria). samples were taken from the top 2 cm of sediments under a fish farm and from a control station in the aquatory of the reservoir in the period july-october 2011. surveillance of bacterial susceptibility to 16 antimicrobial agents was performed for 160 gram-negative strain ... | 2015 | 26019641 |
| bacterial strains isolated from pcb-contaminated sediments and their use for bioaugmentation strategy in microcosms. | this study was focused on the characterization of 15 bacterial strains isolated from long-term pcb-contaminated sediment located at the strážsky canal in eastern part of slovakia, in the surroundings of a former pcb producer. pcb-degrading strains were isolated and identified as microbacterium oleivorans, stenotrophomonas maltophilia, brevibacterium sp., ochrobactrum anthropi, pseudomonas mandelii, rhodococcus sp., achromobacter xylosoxidans, stenotrophomonas sp., ochrobactrum sp., pseudomonas a ... | 2014 | 23553615 |
| anti-influenza virus activity of high-mannose binding lectins derived from genus pseudomonas. | lectin pfl binding high-mannose glycan derived from pseudomonas fluorescens and other homologous lectins: pml derived from pseudomonas mandelii and ptl derived from pseudomonas taiwanensis were examined for antiviral activity. the cdna of these lectin genes were synthesized, cloned, expressed in escherichia coli. the expressed lectins were purified by gel filtrations, and supplied to cultures infected with several strains of influenza virus. these three lectins have inhibited propagation of infl ... | 2016 | 27374061 |
| an organic solvent-tolerant alkaline lipase from cold-adapted pseudomonas mandelii: cloning, expression, and characterization. | a gene encoding a novel organic solvent-tolerant alkaline lipase, lips (genbank id jq071496), was cloned from cold-adapted pseudomonas mandelii. recombinant lips was expressed in escherichia coli as a 32-kda soluble protein and was purified by standard procedures. it maintained more than 80% of its activity under alkaline conditions, ph 8-10.5, with an apparent optimum temperature range of 40-50 °c. it maintained thermal stability from 4 to 50 °c. after 1 h of incubation at 60 °c, approximately ... | 2013 | 23391923 |
| cloning, expression, and characterization of a recombinant esterase from cold-adapted pseudomonas mandelii. | a gene coding for the extracellular esterase (estk) was cloned from the psychrotrophic bacterium pseudomonas mandelii based on its partial amino acid sequence as determined by mass spectrometry. the entire open reading frame consisting of 1,011 bp was expressed in escherichia coli as a soluble protein and purified by nickel-chelated affinity chromatography and capto q column chromatography. here, we show that the 33-kda recombinant estk protein (restksp) had a substrate preference for esters of ... | 2013 | 23117417 |
| beneficial native bacteria improve survival and mycorrhization of desert truffle mycorrhizal plants in nursery conditions. | sixty-four native bacterial colonies were isolated from mycorrhizal roots of helianthemum almeriense colonized by terfezia claveryi, mycorrhizosphere soil, and peridium of t. claveryi to evaluate their effect on mycorrhizal plant production. based on the phylogenetic analysis of the 16s rdna partial sequence, 45 different strains from 17 genera were gathered. the largest genera were pseudomonas (40.8 % of the isolated strains), bacillus (12.2 % of isolated strains), and varivorax (8.2 % of isola ... | 2016 | 27262434 |
| flexibility and stability trade-off in active site of cold-adapted pseudomonas mandelii esterase estk. | cold-adapted enzymes exhibit enhanced conformational flexibility, especially in their active sites, as compared with their warmer-temperature counterparts. however, the mechanism by which cold-adapted enzymes maintain their active site stability is largely unknown. in this study, we investigated the role of conserved d308-y309 residues located in the same loop as the catalytic h307 residue in the cold-adapted esterase estk from pseudomonas mandelii. mutation of d308 and/or y309 to ala or deletio ... | 2016 | 27259687 |
| synthesis and larvicidal activity of low-temperature stable silver nanoparticles from psychrotolerant pseudomonas mandelii. | applications based on silver nanoparticles (agnps) are limited by low temperatures, which cause aggregation of the nanoparticle fraction, leading to reduced efficacy of their products. we aimed at studying agnp synthesis by psychrotolerant bacteria, its stability under long-term storage, and larvicidal activity under low-temperature conditions. electron and atomic force microscopy studies revealed that 6 among 22 psychrotolerant isolates synthesized agnps with an average diameter of 1.9-14.1 nm. ... | 2015 | 25342455 |
| genome sequence of pseudomonas mandelii pd30. | the genome sequence of pseudomonas mandelii pd30 is reported in this announcement. the genes for the reduction of nitrate to dinitrogen were identified in the genome assembly and subsequently used in gene expression research. | 2014 | 25035335 |
| enhanced catalytic site thermal stability of cold-adapted esterase estk by a w208y mutation. | hydrophobic interactions are known to play an important role for cold-adaptation of proteins; however, the role of amino acid residue, trp, has not been systematically investigated. the extracellular esterase, estk, which was isolated from the cold-adapted bacterium pseudomonas mandelii, has 5 trp residues. in this study, the effects of trp mutation on thermal stability, catalytic activity, and conformational change of estk were investigated. among the 5 trp residues, w(208) was the most crucial ... | 2014 | 24667115 |
| nonspecific cleavage of proteins using graphene oxide. | in this article, we report the intrinsic catalytic activity of graphene oxide (go) for the nonspecific cleavage of proteins. we used bovine serum albumin (bsa) and a recombinant esterase (restkp) from the cold-adapted bacterium pseudomonas mandelii as test proteins. cleavage of bsa and restkp was nonspecific regarding amino acid sequence, but it exhibited dependence on temperature, time, and the amount of go. however, cleavage of the proteins did not result in complete hydrolysis into their cons ... | 2014 | 24508487 |
| structure of the o-polysaccharide of pseudomonas mandelii cyar1 containing 3,6-dideoxy-4-c-[(s)-1-hydroxyethyl]-d-xylo-hexose (yersiniose a). | the o-polysaccharide isolated by mild acid hydrolysis of the lipopolysaccharide of pseudomonas mandelii cyar1 was studied by sugar analysis and 1d and 2d (1)h and (13)c nmr spectroscopies. the following structure of the o-polysaccharide was established: | 2013 | 24096274 |
| increased thermal stability of cold-adapted esterase at ambient temperatures by immobilization on graphene oxide. | in this study, the effect of graphene oxide (go) on the thermal stability of a recombinant esterase from cold-adapted pseudomonas mandelii, restkp, was investigated. the complex go-restkp was formed by cross-linking. both free restkp and go-restkp complex showed similar optimum ph and temperatures. go-restkp complex exhibited enhanced thermal stability at ambient temperatures than restkp, which prevented the denaturation of the enzyme by hydrophilic interactions. however, the catalytic efficienc ... | 2013 | 24080443 |
| psychrotrophic pseudomonas mandelii cbs-1 produces high levels of poly-β-hydroxybutyrate. | a novel facultative psychrotroph (strain cbs-1), which accumulates poly-β-hydroxybutyrate (phb), was isolated from soil samples taken from changbai mountain, china. phylogenetic analysis based on 16s rrna sequence data and biolog analysis identified strain cbs-1 as pseudomonas mandelii. transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)h-nuclear magnetic resonance analysis revealed that the granules were composed of phb. p. mandelii cbs-1 grew op ... | 2013 | 24010027 |
| changes in soil microbial community structure with planting years and cultivars of tree peony (paeonia suffruticosa). | to understand the effects of planting tree peony (paeonia suffruticosa) on soil microbial community structure, soil samples were collected from the tree peony gardens with three peony cultivars and three planting years, and adjacent wasteland at luoyang, henan province of china. soil microbial communities were analyzed by the polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) of partial 16s rdna and sequencing methods. with the succeeding development of tree peony garde ... | 2014 | 23933807 |
| purification and properties of an extracellular esterase from a cold-adapted pseudomonas mandelii. | an extracellular esterase, estk, was purified from the psychrotrophic bacterium pseudomonas mandelii grown at 25°c. prior to harvest, cells were treated with 0.2 m mgcl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. estk was purified to homogeneity using standard procedures. it had substrate specificity towards esters of short-chain fatty acids, particularly, p-nitrophenyl acetate. optimum activity of estk was at 40°c; at 4° ... | 2012 | 22315100 |
| cleaning and disinfection of biofilms composed of listeria monocytogenes and background microbiota from meat processing surfaces. | surfaces of food processing premises are exposed to regular cleaning and disinfection (c&d) regimes, using biocides that are highly effective against bacteria growing as planktonic cells. however, bacteria growing in surface associated communities (biofilms) are typically more tolerant towards c&d than their individual free cells counterparts, and survival of pathogens such as listeria monocytogenes may be affected by interspecies interactions within biofilms. in this study, pseudomonas and acin ... | 2017 | 28667108 |
| a bacterium with close genetic identity to pseudomonas mandelii associated with spring fish kills in wild bluegill lepomis macrochirus rafinesque and pumpkinseed sunfish lepomis gibbosus (linnaeus). | pseudomonas fluorescens are known bacterial pathogens in fish. the p. fluorescens group contains at least nine different bacterial species, although species from fish have rarely been differentiated. two isolated fish kills affecting wild bluegills, lepomis macrochirus rafinesque, and pumpkinseed sunfish, lepomis gibbosus (linnaeus), occurred in the spring of 2015 during cool water temperatures (12.5°c-15.5°c). disease signs included severe bacteraemia with rare gross external signs. pure bacter ... | 2017 | 28449322 |