Publications

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lymphocystis in cultured snapper (pagrus auratus) and wild kingfish (seriola lalandi) in australia. 19921627088
observations on the pigmentation of the monogeneans, anoplodiscus spp. (family anoplodiscidae) in different microhabitats on their sparid teleost hosts.the monogeneans anoplodiscus australis and a. cirrusspiralis infect the fins of their respective hosts, the yellowfin bream, acanthopagrus australis, and the snapper, pagrus auratus. in these habitats the adult parasite contains melanin-like pigment deposits within the body. very young parasites lack the pigment but deposits of pigment appear and coalesce into dense deposits within the parasite as it grows. the evidence suggests that the pigment is synthesized within the body of the parasite rat ...19921644520
evidence that the inhibitory effects of stress on reproduction in teleost fish are not mediated by the action of cortisol on ovarian steroidogenesis.ovarian follicles of goldfish, common carp, and the sparid pagrus auratus (new zealand snapper) were incubated in vitro to assess the effects of cortisol (f) on ovarian steroidogenesis. unstimulated goldfish follicles produced little testosterone (t) or 17 beta-estradiol (e2), whereas both carp and snapper follicles spontaneously produced e2 and to a lesser extent t. goldfish follicles produced increased amounts of e2 in response to treatment with human chorionic gonadotropin (hcg), whereas carp ...19958536936
population dynamics of the monogenean anoplodiscus cirrusspiralis on the snapper, pagrus auratus.populations of anoplodiscus cirrusspiralis were monitored for 1 year on tagged individual snapper in experimental cages kept in a large on-shore pond with flow-through filtered sea water. the cages were stocked with small and large fish at either low or high initial density. irrespective of size and density, snapper with light initial infections maintained light infections, whereas fish with heavy initial infections showed fluctuations in parasite population size throughout the year. these data ...19989602377
a screen of mammalian antibodies on snapper (pagrus auratus, sparidae) peripheral blood leukocytes reveals cross reactivity of an anti-human cd3 antibody with a population of mig(-) cells.detailed immunological studies of the teleosts have been hampered by a lack of antibodies against cell-specific markers. furthermore, where antibodies have been raised, in many instances they have been found to be species-specific. in comparison, many monoclonal and polyclonal antibodies exist with specificities for mammalian proteins and glycoproteins that effectively differentiate leukocyte sub-populations. in this study, we have tested a panel of 54 commercial antibodies against human and mur ...200111472777
isolation and partial characterization of a pentraxin-like protein with complement-fixing activity from snapper (pagrus auratus, sparidae) serum.pentraxin-like molecules have been isolated from a number of fish species. however, little is known about the function of these proteins in the teleosts. in this study we report the isolation and characterization of a pentraxin-like molecule from the serum of snapper (pagrus auratus) that has the ability to activate complement. this pentraxin-like protein was isolated from serum by calcium-dependent binding to agarose. sds-page analysis demonstrated an oligomeric protein of approximately 200k da ...200312697314
development and validation of a radioimmunoassay for fish insulin-like growth factor i (igf-i) and the effect of aquaculture related stressors on circulating igf-i levels.this paper describes the development and validation of a commercially available radioimmunoassay (ria) for the detection of fish insulin-like growth factor-i (igf-i). the assay was developed using recombinant barramundi igf-i as antigen and recombinant tuna igf-i as radiolabelled tracer and standard. assay sensitivity was 0.15 ng/ml, inter-assay variation was 16% (n = 9) and intra-assay variation was 3% (n = 10). cross reactivity of less than 0.01% was found with salmon insulin, salmon igf-ii an ...200414723878
assessment of snapper (pagrus auratus) natural igm binding to bromelain treated sheep erythrocytes.normal snapper (pagrus auratus bloch and schneider) serum was examined for natural igm that binds to protease (bromelain) treated sheep erythrocytes (brsrbc) in a model assay system that has been used to appraise natural igm of various mammals. normal snapper serum lysed brsrbc while haemolysis was abrogated by heat inactivation of serum and in divalent cation-deficient conditions, indicative of classical complement mediated lysis. in addition, heat inactivated normal snapper serum agglutinated ...200515450972
the opsonising activity of a pentraxin-like protein isolated from snapper (pagrus auratus, sparidae) serum.in mammals the pentraxins, c-reactive protein (crp) and serum amyloid p component (sap) are important components of the immune response. although pentraxins have been isolated from a number of fish species few studies detail their functional immunological role. in this paper we report the establishment of a flow cytometry based assay to measure the phagocytic activity of isolated snapper head kidney leukocytes (hkls). this assay was then used to examine the ability of a pentraxin-like protein is ...200515854682
infection with photobacterium damselae subspecies damselae and vibrio harveyi in snapper, pagrus auratus with bloat.to diagnose the cause of chronic, low mortality associated with bloat in tanks of snapper at an aquaculture facility.200616739527
cascading effects of fishing can alter carbon flow through a temperate coastal ecosystem.mounting evidence suggests that fishing can trigger trophic cascades and alter food web dynamics, yet its effects on ecosystem function remain largely unknown. we used the large-scale experimental framework of four marine reserves, spanning an oceanographic gradient in northeastern new zealand, to test the extent to which the exploitation of reef predators can alter kelp carbon flux and secondary production. we provide evidence that the reduction of predatory snapper (pagrus auratus) and lobster ...200819263885
speciation of gram-positive bacteria in fresh and ambient-stored sub-tropical marine fish.this study identified gram-positive bacteria in three sub-tropical marine fish species; pseudocaranx dentex (silver trevally), pagrus auratus (snapper) and mugil cephalus (sea mullet). it further elucidated the role played by fish habitat, fish body part and ambient storage on the composition of the gram-positive bacteria. a total of 266 isolates of gram-positive bacteria were identified by conventional biochemical methods, vitek, pcr using genus- and species-specific primers and/or 16s rrna gen ...201020110133
molecular cloning and characterization of adult sparus aurata hemoglobin genes.among teleosts, sparus aurata occupies a prominent place in the gastronomic and economic fields of the mediterranean basin and other geographic districts. the knowledge of its molecular structures and functional features, such as hemoglobin, may be helpful to understand the adaptive biochemical mechanisms that allow this fish to live under extreme conditions, including fish farming. in sparus aurata red blood cells two different alpha and one beta hemoglobin genes have been identified. the alpha ...201020210659
histological identification of osteocytes in the allegedly acellular bone of the sea breams acanthopagrus australis, pagrus auratus and rhabdosargus sarba (sparidae, perciformes, teleostei).the bone of advanced teleost fishes such as those of the family sparidae is said to lack osteocytes or to be acellular. acellularity has been determined by apparent lack of osteocyte lacunae. this study questions the validity of this criterion. scanning electron and light microscopy of paraffin and resin sections were used to show that the sides of sea bream mandibles consist of laminar parallel-fibred bone that we call tubular bone, because it contains tubules, and localised regions of sharpey ...19947818089
structure and origin of the tooth pedicel (the so-called bone of attachment) and dental-ridge bone in the mandibles of the sea breams acanthopagrus australis, pagrus auratus and rhabdosargus sarba (sparidae, perciformes, teleostei).scanning electron and light microscopy were used to show that the pedicels of fish teeth (the so-called "bones of attachment") consist of three types of dentine that lie concentrically around a pulp cavity lined with typical odontoblasts with cytoplasmic processes in dentinal tubules. circumpulpal canalar dentine forms on a thin layer of orthodentine that is encased in mantle dentine. canalar dentine is a new name given to a dentine that is similar to vasodentine in canal arrangement, but not ap ...19948192237
the origin and evolution of the surfactant system in fish: insights into the evolution of lungs and swim bladders.several times throughout their radiation fish have evolved either lungs or swim bladders as gas-holding structures. lungs and swim bladders have different ontogenetic origins and can be used either for buoyancy or as an accessory respiratory organ. therefore, the presence of air-filled bladders or lungs in different groups of fishes is an example of convergent evolution. we propose that air breathing could not occur without the presence of a surfactant system and suggest that this system may hav ...201015547792
swim bladder function and buoyancy control in pink snapper (pagrus auratus) and mulloway (argyrosomus japonicus).physoclist fish are able to regulate their buoyancy by secreting gas into their hydrostatic organ, the swim bladder, as they descend through the water column and by resorbing gas from their swim bladder as they ascend. physoclists are restricted in their vertical movements due to increases in swim bladder gas volume that occur as a result of a reduction in hydrostatic pressure, causing fish to become positively buoyant and risking swim bladder rupture. buoyancy control, rates of swim bladder gas ...201423979722
method to measure the force to pull and to break pin bones of fish.a texture measurement device was modified to measure the force required to pull pin bones from king salmon (oncorhynchus tshawytscha), snapper (pagrus auratus), and kahawai (arripis trutta). pulled bones were also subjected to tension to measure the breaking force. for all fish, the pulling force depended on the size of the fish, and on the length of the pin bone (p < 0.05). in general, larger fish required greater pulling force to remove pin bones. for example, fresh small salmon (about 1500 g ...201525604165
the influence of temperature on the fluorine and calcium composition of fish scales.proton microprobe studies of the scales of the kahawai (arripis trutta) and the snapper (chrysophrys auratus) showed non-linear changes in the fluorine to calcium ratio that increase with increasing temperature, but both species showed a different temperature sensitivity. fluorine and calcium levels vary within years from summer to winter by up to 1000%, making fluorine and calcium levels good markers of seasonal events.199018620324
species of stephanostomum looss, 1899 (digenea: acanthocolpidae) from fishes of australian and south pacific waters, including five new species.nine species of stephanostomum are described from australian and southern pacific marine fishes: stephanostomum madhaviae n. sp. [syn. s. orientalis of madhavi (1976)] from caranx ignobilis, off hope island, queensland, with 30-34 circum-oral spines and vitelline fields almost reaching to the posterior extremity of the cirrus-sac; s. bicoronatum (stossich, 1883) from argyrosomus hololepidotus, off southport broadwater, queensland; s. votonimoli n. sp. from scomberoides lysan, off moorea, french ...200312878848
three species of pycnadenoides yamaguti, 1938 (digenea: opecoelidae), including two new species from temperate marine fishes of australia.pycnadenoides pagrosomi yamaguti, 1938 and p. reversati n. sp. from pagrus auratus (sparidae) and p. invenustus n. sp. from nemadactylus valenciennesi (cheilodactylidae) are described from the temperate marine waters off south-west western australia and south-east queensland. the difference in the anterior extent of the vitelline follicles observed in p. reversati n. sp. recovered from off south-east queensland waters and the material from off western australia is discussed. p. reversati n. sp. ...200312878851
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