| deep sequencing analysis of apple infecting viruses in korea. | deep sequencing has generated 52 contigs derived from five viruses; apple chlorotic leaf spot virus (aclsv), apple stem grooving virus (asgv), apple stem pitting virus (aspv), apple green crinkle associated virus (agcav), and apricot latent virus (aplv) were identified from eight apple samples showing small leaves and/or growth retardation. nucleotide (nt) sequence identity of the assembled contigs was from 68% to 99% compared to the reference sequences of the five respective viral genomes. sequ ... | 2016 | 27721694 |
| characterization of prunus-infecting apricot latent virus-like foveaviruses: evolutionary and taxonomic implications. | the complete genomic sequences of four prunus-infecting apricot latent virus (aplv) like isolates were determined and used to analyze the taxonomic position and variability of these viruses. the results indicate that all isolates show a typical foveavirus genetic organization. despite an average 23% nucleotide divergence, they show strong colinearity with only three regions of significant indel variability, in the internal and 3' non-coding regions and variable n-terminal half of the coat protei ... | 2010 | 21144869 |
| optimization of cdna amplification of apricot latent virus (aplv) from various plant tissues sources. | although the reverse transcriptase polymerase chain reaction (rt-pcr) procedure is basically simple operation, often it is not possible to achieve optimum results without optimizing the protocols. an rt-pcr method targeting a 200 bp sequence of the cp gene of apricot latent virus (aplv) was used as a model to improve the detection limit and to compare the behavior of three different plant tissues in a rt-pcr assay. a number of factors should be considered when selecting the optimal system for rt ... | 2007 | 19069893 |
| use of dried high-phenolic laden host leaves for virus and viroid preservation and detection by pcr methods. | the efficiency of rna extraction for apricot latent virus (aplv), plum bark necrosis stem pitting associated virus (pbnspav), prunus necrotic ring spot virus (pnrsv), potato virus y (pvy), and apple scar skin viroid (assvd) from infected hosts is of great importance for molecular diagnosis by the polymerase chain reaction (pcr). a method is described for drying tissue to overcome phenolic inhibitors of viral rna. this study showed that the infected host leaves, dried at 65 degrees c for 2 days a ... | 2006 | 16879877 |
| characterization of two different apricot latent virus variants associated with peach asteroid spot and peach sooty ringspot diseases. | peach asteroid spot (pas) and peach sooty ringspot (psrs) are two diseases of stone fruit trees of unknown aetiology. the use of a crna probe of the newly described apricot latent virus (aplv), a tentative member of the foveavirus genus, indicated the presence of cross-hybridizing agents in pas isolate la2 and in psrs isolates caserta 12 and clava j4. analysis of dsrna patterns revealed in each case the presence of a major dsrna band of about 9.6 kbp. the purified dsrnas were used to obtain cdna ... | 2001 | 11676410 |
| apricot latent virus: a new species in the genus foveavirus. | extraction of viral double-stranded rna from peach leaves infected with apricot latent virus (alv) followed by molecular cloning of synthesized cdna and its sequencing, suggested that alv is a new virus, whose coat protein (cp) coding region contains apple stem pitting virus (aspv)-related sequences. the sequenced portion of the alv genome (1,444 nt) includes the putative cp gene and the 3' non-translated region. the 5' portion of this fragment (1-651 nt) is highly distinct whereas the 3' portio ... | 2000 | 11043942 |