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redescription of two species of the genus gyrodactylus nordmann, 1832 (monogenoidea) from misgurnus anguillicaudatus.a redescription is given of gyrodactylus macracanthus hukuda, 1940 and g. micracanthus hukuda, 1940. g. paralatus gussev, 1955 was placed in synonymy with the species g. macracanthus. specimens determined by ergens and dulmaa (1968) to be g. paralatus are considered to be identical to g. micracanthus.19751222919
two human cases of gnathostomiasis and discovery of a second intermediate host of gnathostoma nipponicum in japan.two human cases of gnathostomiasis from ingestion of raw native japanese loaches, misgurnus anguillicaudatus, are reported. seven early third-stage larval gnathostoma nipponicum were recovered from 3,098 loaches in the same district in which 2 human patients had obtained and eaten raw loaches. encapsulated g. nipponicum larvae were also recovered from loaches infected under laboratory conditions. all 6 weasels captured in the same district in which the naturally infected loaches were found and w ...19883397824
larval gnathostoma nipponicum found in the imported chinese loaches.six early third-stage larvae of gnathostoma nipponicum were recovered from the muscle of 376 loaches, misgurnus anguillicaudatus, imported from china. they were 614 x 114 microns in average size, almost colorless except brownish intestine, and encircled by about 229 transverse rows of minute cuticular spines. their head bulbs provided with 3 rows of hooklets, of which average number were 34.5 on the first, 36.7 on the second and 39.7 on the third. accordingly, it is revealed that the chinese loa ...19938297892
a novel antimicrobial peptide from the loach, misgurnus anguillicaudatus.a novel antimicrobial peptide, named misgurin, was isolated and characterized from the loach (mudfish), misgurnus anguillicaudatus. the 21-amino-acid peptide with a molecular mass of 2502 da was purified to homogeneity using a heparin-affinity column and c18 reverse-phase and gel-permeation high-performance liquid chromatography. the complete amino acid sequence of misgurin, which was determined by an automated amino acid sequencer, was arg-gln-arg-val-glu-glu-leu-ser-lys-phe-ser-lys-lys-gly-ala ...19979271200
concurrent invaders--four exotic species of monogenea now established on exotic freshwater fishes in australia.four species of exotic monogeneans are reported from five species of exotic freshwater fish in australia: gytrodactylus bullatarudis from poecilia reticulata and xiphophorus helleri in queensland; gyrodactylus macracanthus from misgurnus anguillicaudatus in the australian capital territory; dactylogyrus extensus from cyprinus carpio in the australian capital territory; and dactylogyrus anchoratus from carassius auratus in the australian capital territory. this is the first published record of de ...19989846613
massaliatrema misgurni n. sp. (trematoda: heterophyidae) whose metacercariae encyst in loaches (misgurnus anguillicaudatus).hitherto unknown metacercariae were found encysted in loaches (misgurnus anguillicaudatus) from china. they were experimentally fed to golden hamsters, and gravid adults were recovered 1 week post-infection from their small intestines. a new species, massaliatrema misgurni n. sp. (heterophyidae), is described from the adults. this new species is different from m. gyrinicola dollfus and timon-david, 1960, in having a smaller acetabulum/oral sucker ratio, less branched vitellaria widely entering t ...200111719113
the preparation and characterization of an antimicrobial polypeptide from the loach, misgurnus anguillicaudatus.using sephadex g-50 gel filtration, deae-52 cellulose ion-exchange chromatography, and an improved polyacrylamide gel electrophoresis together with electroelution, a novel polypeptide with antimicrobial activity in vitro was isolated and characterized from loach, misgurnus anguillicaudatus. the polypeptide, named mapp, contains about 94 residues containing l0 different amino acids, of which cysteine was the most abundant. no alkaline residue was found in mapp. mapp is a single-chain polypeptide ...200212406677
[epidemiological studies of clonorchis sinensis prevailed in the peoples of kyungpook province]the author studied the infection rates of clonorchiis sinensis in the inhabitants of kyungpook province, and surveyed infestation rates of clonorchis metacercarias in the twelve kinds of fresh water fishes for the purpose of the eradication. plan of clonorchiasis of kyungpook province. the examination was continued from april 1960 to october 1962 with 24,252 inhabitants ranging from four months to over sixty aged. all the family were tested intradermally with clonorchis antigen and then confirme ...196412913603
camallanus cotti (nematoda: camallanidae), an introduced parasite of fishes in new caledonia.a pathogenic asian nematode species of camallanus, c. cotti fujita, 1927, was found in new caledonia, south pacific, for the first time; it was recorded from two native fishes, awaous guamensis (valenciennes) (gobiidae) (prevalence 51%, intensity 1-25) and kuhlia marginata (cuvier) (kuhliidae) (a single specimen found), of the la foa river, about 100 km north of nouméa; the latter represents a new host record. morphological data on c. cotti based on new caledonian specimens and those previously ...200617252923
protective effect of misgurnus anguillicaudatus polysaccharide on immunological liver injury in mice.the modulation influence of misgurnus anguillicaudatus polysaccharide on the expression of nitric oxide synthase (nos), b cell lymphoma/leukemia-2 (bcl-2, hepatocyte apoptosis inhibitor) and bcl-2 associated x protein (bax, hepatocyte apoptosis promoter) in mice's liver with immunological hepatic injury was studied. immunological hepatic injury was induced by lipopolysaccharide (lps ip, 0.2 mg kg(-1)) in bacillus calmette-guerin (bcg ip, 0.15 g kg(-1), once, before 7 days) primed mice. the mice ...200818387502
a sperm cryopreservation protocol for the loach misgurnus anguillicaudatus and its applicability for other related species.the aim of the present study was to establish a protocol of sperm cryopreservation in misgurnus anguillicaudatus and verify the applicability of the obtained protocol in other loach species. we evaluated the following parameters: inseminating dose, thawing temperatures (20, 25 and 30 degrees c for 10s), extenders (loach or cyprinid extenders), internal cryoprotectants (dimethyl sulfoxide (dmso), dimethylacetamide (dma), glycerol (gly), ethylene glycol (eg), and methanol (meoh) at 0, 5, 10 and 15 ...200919446413
bioaccumulative characteristics of hexabromocyclododecanes in freshwater species from an electronic waste recycling area in china.hexabromocyclododecanes (hbcds) are now emerging ubiquitous contaminants due to their wide usage, persistence and toxicities. to investigate the bioaccumulative characteristics of hbcds, sediments, winkle (littorina littorea), crucian carp (carassius carassius) and loach (misgurnus anguillicaudatus) were collected from two streams near an e-waste dismantling site in china, and hbcd exposure test was then conducted on chinese rare minnow. the concentration of hbcds was 14 ng g(-1) dry weight in s ...200919616822
genetics of the loach, misgurnus anguillicaudatus: recent progress and perspective.the loach misgurnus anguillicaudatus is an interesting fish from the viewpoint of polyploidy and its related atypical reproduction. among japanese populations, bisexual diploid individuals are most common, but natural triploids have been recorded in some localities. experimental crosses including induced gynogenesis and inter-specific hybridization showed that triploid loaches arose from unreduced eggs formed by sympatric diploids. two types of unreduced eggs produced by these diploid females, b ...200315303349
a new gyrodactylid species from cobitis granoei (rendahl) (cobitidae) in central china.gyrodactylus granoei n. sp. (monogenea: gyrodactylidae) is described from the fins and body surface of the spine loach, cobitis granoei (rendahl) (cobitidae), in central china. it resembles a suite of species from cyprinid and cobitid fishes that have short, compact hamuli, a ventral bar with no obvious anterolateral projections, a linguiform posterior membrane, a male copulatory organ with small hooks in multiple rows, a simple cylindrical dorsal bar, and short marginal hooks, with an expanded ...201020950095
production of loach (misgurnus anguillicaudatus) germ-line chimera using transplantation of primordial germ cells isolated from cryopreserved blastomeres.an efficient procedure for the cryopreservation of fish blastomeres followed by restoration through germ-line chimera formation was established. blastomeres of the loach (misgurnus anguillicaudatus) were cryopreserved in 250--ál straws in eagle's minimum essential medium with various concentrations of dimethyl-sulfoxide (0, 5, 10, 15, and 20%), and the best concentration was combined with glycerol (1, 2, and 4%) and external cryoprotectants (1 or 2% sucrose; 2, 5, or 10% fetal bovine serum; 1 or ...201121398566
protective effect of polysaccharide from the loach on the in vitro and in vivo peroxidative damage of hepatocyte.misgurnus anguillicaudatus polysaccharide (map), a type of natural neutral polysaccharide occurring in the mucus of misgurnus anguillicaudatus c., was studied for antioxidant bioactivity. the preliminary research showed that map was able to remove o(2)*, ho., h(2)o(2) and other active compounds of oxygen and significantly protected dna chains from being damaged by hydroxyl radicals. this antioxidant bioactivity was further evaluated using the model of peroxide-induced oxidative injury in rat pri ...200212550070
geographical variation of lactate dehydrogenase and phosphoglucomutase in the loach misgurnus anguillicaudatus.one of the two loci controlling muscle lactate dehydrogenase (ldh) was shown to be polymorphic in the loach misgurnus anguillicaudatus. the result of the survey on four populations suggests that the differences in the ldh gene frequencies are correlated with the geographical distribution of the loach. a fourth phosphoglucomutase allele (pgmd) was found in one of the loach populations examined.2003161953
survey for gnathostoma nipponicum larvae in gobiid freshwater fish and infectivity of the larvae to a gobiid fish (chaenogobius urotaenia).from june 1993 to november 1996, a total of 977 gobiid fish consisting of three species (tridentiger brevispinis, chaenogobius urotaenia, and rhinogobiu brunneus) collected from eastern aomori prefecture, were examined for gnathostoma nipponicum larvae infection. only one species, c. urotaenia was infected with advanced third-stage larvae (adl3), and a total of 22 larvae were recovered from 17 (3.4%) of 500 fish. the infected fish were larger than 12 cm in body length and collected in the may-ju ...19979300363
establishment and characterization of a skin epidermal cell line from mud loach, misgurnus anguillicaudatus, (mase) and its interaction with three bacterial pathogens.a continuous skin epidermal cell line from mud loach (misgurnus anguillicaudatus) (mase cell line) was established with its application in bacteria infection demonstrated in this study. primary mase cell culture was initiated at 26 °c in dulbecco's modified eagle medium/f12 medium (1:1; ph7.2) supplemented with 20% fetal bovine serum (fbs). the primary mase cells in spindle morphology proliferated into a confluent monolayer within 2 weeks, and were continuously subcultured even in 10% fbs- dmem/ ...201627288257
electron microscope study of vertebrate liver innervation.liver fine structure was studied in various groups of vertebrates to reveal intrahepatic nerves. nerve fibers were found in the connective tissue of the liver in all mammals, birds, and reptiles studied (japanese monkey, crab-eating monkey, rabbit, guinea pig, rat, golden hamster, pigeon, japanese quail, and turtle, pseudemys scripta). nerve fibers also made direct contact with hepatocytes in these animals except for the rat and the golden hamster. intrahepatic nerves were rare or absent in amph ...19817316686
polymorphonuclear leukocyte-mediated cytolysis induced by animal lectin.nine animal lectins, i.e., sarcophaga peregrina agglutinin, balanus roseus agglutinin, aplysia kurodai agglutinin, balanus balanoides agglutinin, tetraclita squamosa japonica agglutinin, misgurnus anguillicaudatus lectin, asterina pectinifera agglutinin, helix aspersa agglutinin and helix pomatia agglutinin, were tested for induction of cytolysis mediated by polymorphonuclear leukocytes. among them, s. peregrina agglutinin and b. roseus agglutinin lysed murine target cells in co-operation with p ...19836628907
molecular cloning, characterization, and expression of hsp60 in caudal fin regeneration of misgurnus anguillicaudatus.urodele amphibians and teleost fish are capable of nearly perfect regeneration of lost appendages. the fin constitutes an important model for studying the molecular basis of tissue regeneration. it has been known that heat shock protein 60 (hsp60) is a multifunctional protein of the heat shock protein family. the purpose of this study is to investigate the role of hsp60 as a part of a stress response system after fin injury or in fin regeneration. we firstly cloned full-length cdna of hsp60 from ...201424257806
comparison of haematology and serum biochemistry of cultured and wild dojo loach misgurnus anguillicaudatus.haematological and serum biochemistry parameters were studied and compared between cultured and wild ecotypes of dojo loach misgurnus anguillicaudatus during the prime fishing season, i.e. may-august. data were analysed for the impact of feeding regime and other ecological conditions on the physiology of fish. the results revealed that haemoglobin, cholesterol, total protein, creatinine and uric acid levels in the two ecotypes were significantly different (n = 56, df = 54, p < 0.05). in addition ...200918998231
evidence for the transfer of polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins, and polychlorinated dibenzofurans from soil into biota.the concentrations of six indicator and co-planar polychlorinated biphenyls (pcbs), 17 polychlorinated dibenzo-p-dioxins, and polychlorinated dibenzofurans (pcdd/fs) were determined in soil and associated biota samples collected from a polluted and wasted farmland in southern china. the sum of six indicator pcbs (nos 28, 52, 101, 138, 153 and 180) concentrations in biota samples were proportional to the total amount of pcbs that transferred from soil to the corresponding biota samples, and the s ...200616574199
molecular cloning and sequence analysis of full-length growth hormone cdnas from six important economic fishes.in this study,the full-length cdnas of gh (growth hormone) gene was isolated from six important economic fishes, siniperca kneri, epinephelus coioides, monopterus albus, silurus asotus, misgurnus anguillicaudatus and carassius auratus gibelio bloch. it is the first time to clone these gh sequences except e. coioides gh. the lengths of the above cdnas are as follows: 953 bp, 1 023 bp, 825 bp, 1 082 bp, 1 154 bp and 1 180 bp. each sequence includes an orf of about 600 bp which encodes a protein of ...200515715434
apoptosis induction on hl-60 cells of a novel polysaccharide from the mucus of the loach, misgurnus anguillicaudatus.this study was designed to determine the anti-proliferative, apoptotic properties of a novel polysaccharide from the loach, misgurnus anguillicaudatus (map), using a human promyelocytic leukemia line (hl-60) as a model system. hl-60 cells were cultured in the presence of map at various concentrations (50-800 mg/l) for 5 days and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay. the results showed that map inhibited the ce ...200515935580
xenogenesis in teleost fish through generation of germ-line chimeras by single primordial germ cell transplantation.primordial germ cells (pgcs) are the only cells in developing embryos with the potential to transmit genetic information to the next generation. pgcs therefore have the potential to be of value for gene banking and cryopreservation, particularly via the production of donor gametes with germ-line chimeras. currently, it is not clear how many pgcs are required for germ-line differentiation and formation of gonadal structures. in the present study, we achieved complete germ-line replacement between ...200817901077
[cloning of hemoglobin alpha-chain cdna sequences from five fishes in cypriniformes].oxygen content in water is one of the limit factors to the fish growing. the tolerance of fish to low oxygen content depends on the affinity of hemoglobin to oxygen. the tempt of transgenic fish study is to transfer hemoglobin gene from the fish that tolerates to low oxygen content in water to the fish sensitive to low oxygen condition. therefore, using hemoglobin gene to breed new varieties may become a main trend in the near future. to explore the underline mechanisms for the variation of hemo ...200312939797
factors affecting the efficiency of somatic cell nuclear transplantation in the fish embryo.procedures to improve somatic cell nuclear transplantation in fish were evaluated. we reported effects of nonirradiated recipient eggs, inactivated recipient eggs, different combinations between recipient eggs and donor cells, duration of serum starvation, generation number, and passage number of donor cells on developmental rates of nuclear transplant (nt) embryos. exposure to 25,000 r of gamma-rays inactivated recipient eggs. single nucleus of cultured, synchronized somatic cell from gynogenet ...200212410600
a cryptic clonal line of the loach misgurnus anguillicaudatus (teleostei: cobitidae) evidenced by induced gynogenesis, interspecific hybridization, microsatellite genotyping and multilocus dna fingerprinting.in memanbetsu town, hokkaido island, japan, a high frequency of natural triploid loaches misgurnus anguillicaudatus (7.4% on average) was detected by flow cytometry for relative dna content. among sympatric diploid females (n=6) from a single population, we found two unique females that laid unreduced diploid eggs. they gave normal diploid progeny even after induction of gynogenesis with genetically inert uv-irradiated sperm. when fertilized with normal loach sperm, some unreduced eggs developed ...200212130809
sperm morphology and distribution of intramembranous particles in the sperm heads of selected freshwater teleosts.the morphology of spermatozoa and the distribution of intramembranous particles (imps) in sperm-head membranes in teleostean fish were examined ultrastructurally to clarify the presence of characteristic arrays (parallelogram or hexagon in packing) of imps. the following four species of fish were used: goldfish (carassius auratus), loach (misgurnus anguillicaudatus), flat bitterling (acheilognatus rhombeus), sweetfish (plecoglossus altivelis). it was demonstrated that spermatozoa of all these fi ...199318621254
[studies on the experimental mode of infections of clonorchis sinensis: iii. studies on the wormicidal effect of external mucous substance of some fresh water fish on the larva of clonorchis sinensis]the author reported in the previous pager on his observation of sudden death of clonorchis sinensis cercaria before penetration into the body of fry such as cyprinus carpio and carassius auratus. and in these studies, the reason behind the fact was investigated and it was found that the mucous substances on the surface of a certain fishes have a strong wormicidal effect to the larva of c. sinensis. 1. when cercariae of c. sinensis were immersed into the mucous substance of gold fish for three mi ...196412913595
[studies on the experimental mode of infections of clonorchis sinensis: ii. experimental infection of clonorchis sinensis cercaria to fry of fresh water fishes]cercariae of clonorchis sinensis were attempted to penetrate into various fry of fresh water fishes in order to examine the conditions of infection and the further development in the fish. the following conclusions were made. 1. cercariae of c. sinensis penetrated into the muscle of the fry of pseudorasbora parva were found to be able to develop. 2. cercariae penetrated into the fry of pseudorasbora parva migrated into the organs of the fry to kill their host. 3. from each dead individual of p. ...196412913594
[experimental studies on the second intermediate hosts of clonorchis sinensis: iii. observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of clonorchis sinensis in fresh-water fish]in order to know relationship between the clavate cells of epidermis and infectivity of metacercariae of c. sinensis. histological investigation of epidermis was carried out on the 26 species of fresh-water fish collected from the main rivers in korea. results were summarized as it follows: 1. in general clavate cell had a single nucleus at its center. however it was observed in liobagrus mediadiposalis and pelteobagrus fuluidraco that clavate cell had two nuclei with and independent so-called l ...197412913471
[the wormicidal substances of fresh water fishes on clonorchis sinensis: ii. preliminary research on the wormicidal substances from mucous substances of various fresh water fishes]the present work which was investigated in july 1979, was to observe the wormicidal effects of the external mucous substances of 9 species of fresh water fishes (cyprinus carpio, parasilurus asotus, anguilla japonica, ophicephalus argus, carassius carassius; golden crusian carp, misgurnus anguillicaudatus, zacco platypus, pseudorasbora parva and carassius carassius; crusian carp) on cercaria, liberated metacercaria and adult of clonorchis sinensis. the mucous substances extracted by ether from t ...198012902746
studies on intestinal tematodes in korea: xvi. infection status of loaches with the metacercariae of echinostoma hortense.the infection status of the loaches, misgurnus anguillicaudatus, with the metacercariae of echinostoma hortense, was studied in korea. a total of 154 loaches purchased at 4 local makets (seoul, kimhae, naju-gun and kangjin-gun) were examined their infection rate as well as the density and location of the metacercariae in the fish body. the results are as follews: the loaches carrying the metecercariae of e. hortense were 64 (41.6%) in total number and the metacercarial density ranged 1-29 per in ...198512888682
nuclear transplantation of somatic cells of transgenic red carp (cyprinus carpio haematopterus).nuclear transplantation was performed using kidney cells, tail fin cells and cultured tail fin cells of 18th passage of the f4 hgh gene transferred red carp (cyprinus carpio haematopterus) as donors and the eggs of loach (misgurnus anguillicaudatus) of huanghe carp (cyprinus carpio haematopterus) as recipients. using loach unfertilized eggs as recipients 0.33% of reconstructed embryos developed in to neural plate from kidney cells, and 0.1% of reconstructed embryos developed in to muscular react ...200212043567
protein polymorphism and geographic variation in the loach misgurnus anguillicaudatus.electrophoretic variations of enzymatic and non-enzymatic proteins were investigated in 8 populations of the loach misgurnus anguillicaudatus. the cathodic hemoglobin and the muscle protein in the region ii were shown to be polymorphic, in addition to the five polymorphic proteins previously reported (the anodic hemoglobin, muscle protein in the region iii, 6-phosphogluconate dehydrogenase, phosphoglucomutase and aspartate aminotransferase; kimura, 1976 & 1977). the muscle proteins in the region ...1978568897
trichodinid ectoparasites (ciliophora: peritricha) from misgurnus anguillicaudatus (cantor) and anodonta woodiana (lea) in china, with descriptions of two new species of trichodina ehrenberg, 1838.during parasitological surveys in chongqing, china, three trichodinids infecting a freshwater fish, misgurnus anguillicaudatus (cantor), and a mollusc, anodonta woodiana (lea), were investigated. two of these species were found to be new. trichodina lechriodentata n. sp., from the fish, was identified by its small-sized body and irregularly rectangular blade, while t. rara n. sp., from the mollusc, is characterised by its overall dimensions, denticle morphology and the presence of a white speckl ...200717294258
caryophyllidean tapeworms (platyhelminthes: eucestoda) from freshwater fishes in japan.the following caryophyllidean tapeworms were found in freshwater fishes from japan (species reported from japan for the first time marked with an asterisk): family caryophyllaeidae: paracaryophyllaeus gotoi (motomura, 1927) from misgurnus anguillicaudatus (cantor); archigetes sieboldi leuckart, 1878 from pseudorasbora parva (temminck et schlegel) and sarcocheilichthys variegatus microoculus mori (new hosts); family lytocestidae: *caryophyllaeides ergensi scholz, 1990 from tribolodon hakuensis (g ...200111817451
[studies on echinostoma spp. in the chungju reservoir and upper streams of the namhan river].the present study was performed to know the epidemiological status of echinostomiasis in the chungju reservoir and upper streams of namhan river, together with an experimental study on the life history of echinostoma hortense. the stool specimens of 169 inhabitants and 473 junior high school students from 5 different villages revealed 3 (0.5%) echinostomatid egg positive cases. e. hortense adult worms were recovered from one patient after a treatment and purgation. for the other two patients, it ...19902099176
ecotoxicological effects of mixed pollutants resulted from e-wastes recycling and bioaccumulation of polybrominated diphenyl ethers in chinese loach (misgurnus anguillicaudatus).to understand potential ecotoxicological effects of electrical and electronic equipment waste (e-waste) recycling and polybrominated diphenyl ethers (pbdes) bioaccumulation in loaches, a semi-field experiment using chinese loach (misgurnus anguillicaudatus) as experimental fish was performed. larval loaches were kept in net-cage for three months in an e-wastes recycling site and a reference site in southeastern china. there was significant difference of the survival rate between the loaches from ...200920131600
cryptic clonal lineages and genetic diversity in the loach misgurnus anguillicaudatus (teleostei: cobitidae) inferred from nuclear and mitochondrial dna analyses.in the loach misgurnus anguillicaudatus, the asexual lineage, which produces unreduced clonal diploid eggs, has been identified. among 833 specimens collected from 54 localities in japan and two localities in china, 82 candidates of other lineage(s) of cryptic clones were screened by examining rflp (restriction fragment length polymorphism)-pcr haplotypes in the control region of mtdna. this analysis was performed because triploid loaches arise from the accidental incorporation of the sperm nucl ...200817578669
[experimental and epidemiological studies on the life cycle of echinostoma hortense asada, 1926 (trematoda: echinostomatidae)]recently there have been some reports on human infections of echinostoma hortense in korea. it was found that a few species of freshwater fishes were playing the role of the second intermediate host of e. hortense. however, molluscan intermediate host has not been identified yet in korea. the present study aimed to establish the life cycle of e. hortense in laboratory. experimental studies such as egg production from the rat, development of the eggs in vitro, exposure of miracidia to freshwater ...198612886089
establishment and characterization of fin cell lines from diploid, triploid, and tetraploid oriental weatherfish (misgurnus anguillicaudatus).continuous fin cell lines from diploid, triploid, and tetraploid oriental weatherfish, misgurnus anguillicaudatus, were established and characterized. the cell lines, designated dimf, trmf, and temf, respectively, were subcultured more than 80 times since initiation in october 2012 and were preserved at the china center for type culture collection as sample numbers c2013109, c2013110, c2013111, respectively. the cell lines consist predominantly of fibroblast-like cells. at the 50th passage, the ...201525721100
mechanism of apoptosis induced by a polysaccharide, from the loach misgurnus anguillicaudatus (map) in human hepatocellular carcinoma cells.the biological activities of the polysaccharide have attracted more and more attention in the biochemical and medical areas due to their anti-cancer effects. to estimate the anti-tumor mechanism of map, a novel polysaccharide from the loach, misgurnus anguillicaudatus, the apoptosis effects of the polysaccharide on the human hepatocellular carcinoma cells (smmc-7721 cells) were studied. the present studies showed that map could induce cell apoptosis which was closely accompanied with an increase ...200615936790
antioxidant and antiproliferative activities of loach ( misgurnus anguillicaudatus ) peptides prepared by papain digestion.loach protein hydrolysates (lph) prepared by papain digestion were fractionated into four fractions, lph-i (mw > 10 kda), lph-ii (mw = 5-10 kda), lph-iii (mw = 3-5 kda), lph-iv (mw < 3 kda), and the in vitro antioxidant and antiproliferative (anticancer) activities of all fractions were determined. lph-iv showed the lowest ic(50) value (16.9 -¦ 0.21 mg/ml) for hydroxyl radical scavenging activity and the highest oxygen radical scavenging capacity (orac) value (reaching 215 -¦ 5.9 mm trolox/100 g ...201121675717
mucochondroid (mucous connective) tissues in the heads of teleosts.the distribution and structure of mucochondroid tissues in the heads of teleosts has been studied in 56 species from 26 families. the tissues could also be called mucous connective tissue and have previously been known as basophilic gelatinous tissue. they are a heterogeneous group of tissues that contain fibroblasts (type example--the subcutaneous mucochondroid of the cobitidae) or hyaline cells (type example--the mucochondroid around the medulla oblongata and spinal cord of rasbora heteromorph ...19883177897
impacts of loach bioturbation on the selective bioaccumulation of hbcdd diastereoisomers and enantiomers by mirror carp in a microcosm.to assess the impacts of bioturbation at the water-sediment interface on the bioaccumulation of hexabromocyclododecane diastereoisomers (hbcdds) by pelagic organisms and the bioisomerization and enantioselectivity therein, we built microcosms containing water, mirror carp (cyprinus carpio), and sediment. the microcosms were sorted into two groups, with or without loach (misgurnus anguillicaudatus) living at the water-sediment interface. a 50-d accumulation test was conducted by spiking the micro ...201627565315
transcriptome analysis of the plateau fish (triplophysa dalaica): implications for adaptation to hypoxia in fishes.triplophysa dalaica, endemic species of qinghai-tibetan plateau, is informative for understanding the genetic basis of adaptation to hypoxic conditions of high altitude habitats. here, a comprehensive gene repertoire for this plateau fish was generated using the illumina deep paired-end high-throughput sequencing technology. de novo assembly yielded 145, 256 unigenes with an average length of 1632 bp. blast searches against genbank non-redundant database annotated 74,594 (51.4%) unigenes encodin ...201525869933
fine structure of monoamine-containing basal cells in the taste buds on the barbels of three species of teleosts.the taste buds on the barbels in three species of teleosts (cyprinus carpio, misgurnus anguillicaudatus, parasilurus asotus) were studied by means of fluorescence- and electron microscopy. intensely yellow-fluorescent cells, which are disk-shaped and located exclusively in a basal position, are observed in the barbel-buds of all fishes examined. the basal cells contain a large number of small clear vesicles approximately 40-60 nm in diameter, which show a tendency to aggregate in the cytoplasm f ...19846713479
[studies on intestinal trematodes in korea xiv. infection status of loaches with metacercariae of echinostoma cinetorchis and their development in albino rats]the metacercariae of echinostoma cinetorchis (trematoda: echinostomatidae) were found infected in the loaches (misgurnus anguillicaudatus) purchased from two local markets in korea. identification of the metacercariae was done after obtaining adult worms from experimentally infected albino rats. brief course of worm development in rats was also observed up to 28th post-infection day. the results are as follows: the metacercariae of e. cinetorchis were detected from 5 (10.9 %) out of 46 loaches e ...198412891011
cdna cloning and expression characterization of serum transferrin gene from oriental weatherfish misgurnus anguillicaudatus.in this study, the cdna coding serum transferrin (stf) of misgurnus anguillicaudatus (mastf) was cloned. mastf cdna is composed of 2326 bp with a 2007 bp open reading frame encoding 668 amino acids. the deduced mastf protein consists of a signal peptide, two lobes (n and c-lobes) and signature motifs of transferrin (tf) family. the results of tissue distribution indicated that mastf mrna was predominantly expressed in the liver. the results indicate that the mastf expression increased significan ...201424673686
co-expression of calcium signaling components in vertebrate taste bud cells.in order to investigate the molecular mechanism of calcium signaling pathways common to the vertebrate gustatory systems, we have analyzed the expression of their molecular components. we first identified a phospholipase c (plc) beta subtype expressed in the taste buds of pond loach (misgurnus anguillicaudatus), designated dplcbeta2, which is closely related to mammalian plcbeta2 shown recently to be expressed in rat taste buds. the taste bud-specific expression of plcbeta2 in a fish species as ...200010729634
identification and virulence properties of aeromonas veronii bv. sobria isolates causing an ulcerative syndrome of loach misgurnus anguillicaudatus. 201626525163
prevalence of clonorchis sinensis infection in freshwater fishes in northeastern china.the prevalence of clonorchis sinensis infection in freshwater fishes was surveyed in heilongjiang province, northeastern china, between august 2011 and september 2013. thirteen species of freshwater fish (n=3221) and one species of shrimp (n=93) were collected from songhua river, nenjiang river and other lakes or ponds in 37 sites of 15 representative cities in heilongjiang province. they were individually examined by digestion technique, and the c. sinensis metacercariae were identified morphol ...201424880648
proteocephalid tapeworms (cestoda: onchoproteocephalidea) of loaches (cobitoidea): evidence for monophyly and high endemism of parasites in the far east.the parasite fauna of loaches (cypriniformes: cobitoidea), a group of small bottom-dwelling freshwater fishes with a mostly eurasian distribution, remains a largely unknown quantity. here we revise the taxonomy of tapeworms of the genus proteocephalus weinland, 1858 (cestoda: proteocephalidae) that had been found in loaches from the palaearctic region (central europe, japan and russia [primorsky region]). molecular phylogenetic analysis based on two nuclear (ssr- and lsrdna) and two mitochondria ...201727702602
a restoration-promoting integrated floating bed and its experimental performance in eutrophication remediation.numerous studies on eutrophication remediation have mainly focused on purifying water first, then restoring submerged macrophytes. a restoration-promoting integrated floating bed (rpifb) was designed to combine the processes of water purification and macrophyte restoration simultaneously. two outdoor experiments were conducted to evaluate the ecological functions of the rpifb. trial 1 was conducted to compare the eutrophication purification among floating bed, gradual-submerging bed (gsb) and rp ...201425079639
resting metabolism and heat increment of feeding in mandarin fish (siniperca chuatsi) and chinese snakehead (channa argus).resting metabolism was measured in immature mandarin fish siniperca chuatsi weighing 42.1-510.2 g and chinese snakehead channa argus weighing 41.5-510.3 g at 10, 15, 20, 25, 30 and 35 degrees c. heat increment of feeding was measured in mandarin fish weighing 202.0 (+/-14.0) g and snakehead weighing 200.8 (+/-19.3) g fed swamp loach misgurnus anguillicaudatus at 1% body weight per day at 28 degrees c. in both species, weight exponent in the power relationship between resting metabolism and body ...200011064280
molecular evidence of cryptic diversity in paracaryophyllaeus (cestoda: caryophyllidea), parasites of loaches (cobitidae) in eurasia, including description of p. vladkae n. sp.molecular phylogenetic analysis of an extensive collection of monozoic tapeworms of the genus paracaryophyllaeus kulakovskaya, 1961 (cestoda: caryophyllidea), parasites of loaches (cypriniformes: cobitidae) in eurasia, has revealed cryptic species diversity within this long-time monotypic genus, especially in the paracaryophyllaeus gotoi (motomura, 1927) species complex [syn. paracaryophyllaeus dubininorum (kulakovskaya, 1961); type species]. three independent, well-supported clades were discove ...201425111941
clonal diploid sperm of the diploid-triploid mosaic loach, misgurnus anguillicaudatus (teleostei:cobitidae).the loach misgurnus anguillicaudatus comprises diploid, triploid and diploid-triploid mosaic individuals in a wild population of the hokkaido island, japan. previous studies revealed the presence of a cryptic clonal lineage among diploid loaches, which is maintained by uniparental reproduction of genetically identical diploid eggs. in the present study, we analyzed distribution and genetic status of diploid and triploid cells in infrequent mosaic males. flow cytometry, microsatellite genotyping ...200415181644
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