| rhodanobacter lindaniclasticus gen. nov., sp. nov., a lindane-degrading bacterium. | lindane-degrading activity under aerobic conditions has been observed in two bacterial strains: ut26, phenotypically identified as sphingomonas paucimobilis, and a new single unidentified isolate named rp5557t. the rrs (16s rdna) sequences for both strains and the phenotypic characteristics for the unidentified isolate rp5557t were determined. rp5557t does not have high identity (less than 90% in all cases) with any sequence in the genbank or rdp databases. a phylogenetic analysis based on rrs s ... | 1999 | 10028243 |
| colorimetric assay for lindane dechlorination by bacteria. | a colorimetric microtitre plate-based assay that detects haloalkane dehalogenase activity was modified to detect dechlorination of gamma-hexachlorocyclohexane (lindane). dechlorination is indicated by the colour change of phenol red from red to yellow, in a weakly buffered solution, as the solution becomes acidic due to hcl formed during dechlorination. enzyme activity can be monitored by reading the absorbance of each well at 540 nm. positive controls for the assay were the known lindane-degrad ... | 2001 | 11576682 |
| thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry. | four aerobic, gram-negative bacterial strains isolated from kaolin slurry used in the production of paper were subjected to a polyphasic analysis and characterization to determine their taxonomic position. analysis of the 16s rdna sequences of the four strains revealed that they represent a new lineage within the gamma-proteobacteria, related to the genera xanthomonas, pseudoxanthomonas, stenotrophomonas, luteimonas, xylella and rhodanobacter. analysis of the quinone system, the polyamines, the ... | 2002 | 11931159 |
| fulvimonas soli gen. nov., sp. nov., a gamma-proteobacterium isolated from soil after enrichment on acetylated starch plastic. | six deep-yellow-pigmented strains were isolated from soil after enrichment on plasticized acetylated starch granules as a source of carbon. they showed very similar and unique fatty acid profiles, consisting almost exclusively of branched fatty acids. the strains consisted of small, motile rods, were oxidase- and catalase-positive, did not ferment sugars and were able to depolymerize starch and suspended acetylated starch in overlayer plates. the 16s rdna sequence of a representative strain, str ... | 2002 | 12148641 |
| rhodanobacter sp. strain bpc1 in a benzo[a]pyrene-mineralizing bacterial consortium. | a bacterial consortium which rapidly mineralizes benzo[a]pyrene when it is grown on a high-boiling-point diesel fuel distillate (hbd) was recovered from soil and maintained for approximately 3 years. previous studies have shown that mobilization of benzo[a]pyrene into the supernatant liquid precedes mineralization of this compound (r. kanaly, r. bartha, k. watanabe, and s. harayama, appl. environ. microbiol. 66:4205-4211, 2000). in the present study, we found that sterilized supernatant liquid f ... | 2002 | 12450801 |
| rhodanobacter fulvus sp. nov., a beta-galactosidase-producing gammaproteobacterium. | a taxonomic study was carried out on a bacterial strain designated as jip2t isolated from a soil sample mixed with rotten rice straw. it was a gram-negative, aerobic, motile, and rod-shaped bacterium. it grew well on nutrient agar medium and utilized a fairly narrow spectrum of carbon source. the g+c content of the genomic dna was 65.3 mol%. the major ubiquinone was q-8. the major fatty acids were branched fatty acids, especially large amounts of iso c15:0 and iso c17:1 w9c were detected in the ... | 2004 | 15486823 |
| aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of assam, india. | a bacterial strain designated gptsa 20(t), which was isolated from a warm spring in assam, india, was characterized by using a polyphasic approach. the cells were gram-negative, aerobic rods, which could not utilize or produce acid from most of the carbohydrates tested. the predominant fatty acids were c(15:0) iso (25.04%), c(17:1) iso omega9c (19.28%), c(16:0) iso (17.73%) and c(11:0) iso 3-oh (9.34%). the g+c content was 75 mol%. from 16s rrna gene sequence analysis (1433 nucleotides, continuo ... | 2005 | 16014470 |
| dyella koreensis sp. nov., a beta-glucosidase-producing bacterium. | a bacterial strain (designated bb4(t)), which has beta-glucosidase activity, was isolated from soil around the roots of bamboo plants. cells were gram-negative, aerobic, non-motile and straight-rod-shaped. phylogenetic analysis of 16s rrna gene sequences revealed a clear affiliation with members of the family 'xanthomonadaceae'. the 16s rrna gene sequence of strain bb4(t) showed the following sequence similarities: 97.7% to dyella japonica xd53(t), 97.1% to frateuria aurantia lmg 1558(t), 96.2% ... | 2005 | 16014492 |
| luteibacter rhizovicinus gen. nov., sp. nov., a yellow-pigmented gammaproteobacterium isolated from the rhizosphere of barley (hordeum vulgare l.). | three strains of gram-negative, aerobic, yellow-pigmented, chemo-organotrophic bacteria, motile by a polar flagellum, were isolated from the rhizosphere of spring barley (hordeum vulgare l.) at a research field near copenhagen, denmark. the three strains, lj79, lj96t and lj99, formed visible colonies on one-tenth-strength tryptic soy broth supplemented with agar (1/10 tsba) after incubation for 6 days at 15 degrees c. the strains hydrolysed starch, casein (skimmed milk), gelatin and various pent ... | 2005 | 16280484 |
| rhodanobacter spathiphylli sp. nov., a gammaproteobacterium isolated from the roots of spathiphyllum plants grown in a compost-amended potting mix. | three gram-negative, yellow-pigmented strains were isolated from the rhizospheres of spathiphyllum plants grown in a compost-amended potting mix. the strains showed biological control activity towards the root-rot plant pathogen cylindrocladium spathiphylli, and were characterized to determine their taxonomic position. cells of the strains were non-motile rods, and the strains were oxidase- and catalase-positive and unable to ferment most sugars tested. the three strains showed differences in gr ... | 2006 | 16902003 |
| dyella yeojuensis sp. nov., isolated from greenhouse soil in korea. | a novel strain, r2a16-10(t), was isolated from greenhouse soil in yeoju, korea. the taxonomy of strain r2a16-10(t) was studied by using polyphasic methods. on the basis of 16s rrna gene sequence analyses, strain r2a16-10(t) was found to be phylogenetically related to type strains of dyella species (96.7-96.9 %), frateuria aurantia dsm 6220(t) (96.5 %), fulvimonas soli lmg 19981(t) (96.3 %) and rhodanobacter species (94.9-95.7 %). strain r2a16-10(t), which produced yellow-coloured colonies, was g ... | 2006 | 16957102 |
| rhodanobacter thiooxydans sp. nov., isolated from a biofilm on sulfur particles used in an autotrophic denitrification process. | a novel thiosulfate-oxidizing bacterium, designated strain lcs2(t), was isolated from a biofilm on sulfur particles used in an autotrophic denitrification process. the strain was found to comprise gram-negative, non-motile, non-spore-forming rods that produced yellow-pigmented colonies on r2a agar. the strain contained q-8 as the major ubiquinone and 17 : 1 iso omega 9c, 15 : 0 iso and 17 : 0 iso as the major fatty acids. the g+c content of the genomic dna was 64.6 mol%. the 16s rrna gene sequen ... | 2007 | 17684255 |
| diversity of bacterial isolates from commercial and homemade composts. | the diversity of heterotrophic bacterial isolates of three commercial and two homemade composts was studied. the commercial composts were produced from poultry litter (pc), sewage sludge (sc), municipal solid waste (mc), and homemade composts (thermal compost [dc] and vermicompost [vc]) from food wastes. the taxonomic and physiological diversity of the heterotrophic culturable bacteria was assessed using phenotypic and genotypic characterization and the analysis of the partial 16s rrna gene sequ ... | 2008 | 17876655 |
| rhodanobacter ginsengisoli sp. nov. and rhodanobacter terrae sp. nov., isolated from soil cultivated with korean ginseng. | two bacterial isolates from ginseng fields in korea, strains gr17-7(t) and gp18-1(t), were characterized using a polyphasic approach. phylogenetic analysis of their 16s rrna gene sequences revealed a clear affiliation with the gammaproteobacteria, and showed that the closest phylogenetic relationships were with members of the genus rhodanobacter. the 16s rrna gene sequence similarity between strains gr17-7(t) and gp18-1(t) was 97.2 %. both strains showed 16s rrna gene sequence similarities of 95 ... | 2007 | 18048729 |
| bacterial diversity associated with ornithogenic soil of the ross sea region, antarctica. | in the ross sea region of antarctica, ornithogenic soils form on land under adélie penguin rookeries. compared with mineral soils of the ross sea region, ornithogenic soils are generally high in microbial biomass, organic carbon, and total nitrogen and phosphorus, with high electrical conductivity and large variations in ph. the objective of this study was to assess the bacterial composition of ornithogenic soils from cape hallett and cape bird in the ross sea region using culture-independent me ... | 2009 | 19190698 |
| rhodanobacter ginsenosidimutans sp. nov., isolated from soil of a ginseng field in south korea. | a novel gammaproteobacterium, designated gsoil 3054(t), was isolated from soil of a ginseng field in pocheon province, south korea, and was characterized using a polyphasic approach to determine its taxonomic position. the strain was gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped. phylogenetic analysis based on 16s rrna gene sequences showed that the strain belonged to the genus rhodanobacter; it was most closely related to rhodanobacter fulvus jip2(t) and rhodanobacter thi ... | 2009 | 19329589 |
| composition and activity of an autotrophic fe(ii)-oxidizing, nitrate-reducing enrichment culture. | 16s rrna gene libraries from the lithoautotrophic fe(ii)-oxidizing, nitrate-reducing enrichment culture described by straub et al. (k. l. straub, m. benz, b. schink, and f. widdel, appl. environ. microbiol. 62:1458-1460, 1996) were dominated by a phylotype related (95% 16s rrna gene homology) to the autotrophic fe(ii) oxidizer sideroxydans lithotrophicus. the libraries also contained phylotypes related to known heterotrophic nitrate reducers comamonas badia, parvibaculum lavamentivorans, and rho ... | 2009 | 19749073 |
| polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent. | biological purification processes are effective tools in the treatment of hazardous wastes such as toxic compounds produced in coal coking. in this study, the microbial community of a lab-scale activated sludge system treating coking effluent was assessed by cultivation-based (strain isolation and identification, biodegradation tests) and culture-independent techniques (sequence-aided t-rflp, taxon-specific pcr). the results of the applied polyphasic approach showed a simple microbial community ... | 2010 | 20093025 |
| rhodanobacter soli sp. nov., isolated from soil of a ginseng field. | strain dcy45(t) was isolated from soil of a ginseng field in pocheon province, korea. strain dcy45(t) was gram-negative, oxidase- and catalase-positive, motile and rod-shaped and produced yellow pigments on r2a agar. the organism grew optimally at 30 °c and at ph 7.0. the g+c content of the genomic dna was 65.4 mol%. the predominant respiratory quinone was q-8. the major fatty acids were iso-c(17 : 1)ω9c, iso-c(16 : 0) and iso-c(15 : 0). phylogenetic analysis based on the 16s rrna gene sequence ... | 2010 | 20118296 |
| denitrifying bacteria isolated from terrestrial subsurface sediments exposed to mixed-waste contamination. | in terrestrial subsurface environments where nitrate is a critical groundwater contaminant, few cultivated representatives are available to verify the metabolism of organisms that catalyze denitrification. in this study, five species of denitrifying bacteria from three phyla were isolated from subsurface sediments exposed to metal radionuclide and nitrate contamination as part of the u.s. department of energy's oak ridge integrated field research challenge (or-ifrc). isolates belonged to the gen ... | 2010 | 20305024 |
| denitrification at ph 4 by a soil-derived rhodanobacter-dominated community. | soil denitrification is a major source of nitrous oxide emission that causes ozone depletion and global warming. low soil ph influences the relative amount of n₂o produced and consumed by denitrification. furthermore, denitrification is strongly inhibited in pure cultures of denitrifying microorganisms below ph 5. soils, however, have been shown to denitrify at ph values as low as ph 3. here we used a continuous bioreactor to investigate the possibility of significant denitrification at low ph u ... | 2010 | 20649643 |
| significant association between sulfate-reducing bacteria and uranium-reducing microbial communities as revealed by a combined massively parallel sequencing-indicator species approach. | massively parallel sequencing has provided a more affordable and high-throughput method to study microbial communities, although it has mostly been used in an exploratory fashion. we combined pyrosequencing with a strict indicator species statistical analysis to test if bacteria specifically responded to ethanol injection that successfully promoted dissimilatory uranium(vi) reduction in the subsurface of a uranium contamination plume at the oak ridge field research center in tennessee. remediati ... | 2010 | 20729318 |
| rhodanobacter xiangquanii sp. nov., a novel anilofos-degrading bacterium isolated from a wastewater treating system. | a gram-staining-negative, oxidase-positive, catalase-positive, non-motile, non-spore-forming, and rod-shaped bacterium, designated bjq-6(t), was isolated from activated sludge of a waste-water treatment plant in jiangsu province, china. phylogenetic analysis based on 16s rrna gene sequences showed that strain bjq-6(t) belonged to the genus rhodanobacter, and shared 98.7% similarity with rhodanobacter lindaniclasticus lmg 18385(t) and <97% similarities with other rhodanobacter species. the major ... | 2010 | 20936473 |
| rhodanobacter panaciterrae sp. nov., with ginsenoside converting activity isolated from soil of a ginseng field. | a novel gammaproteobacterium, designated lnr5-47t, was isolated from soil of a ginseng field in liaoning province, china and was characterized using a polyphasic approach. the strain was gram-staining-negative, aerobic, non-motile, non-spore-forming, and rod-shaped. phylogenetic analysis based on 16s rrna gene sequences showed that this strain belonged to the genus rhodanobacter; it is most closely related to the rhodanobacter ginsengisoli gr17-7t, rhodanobacter terrae gp18-1t, dyella ginsengiso ... | 2011 | 21296929 |
| characterization of a novel ginsenoside-hydrolyzing α-l: -arabinofuranosidase, abfa, from rhodanobacter ginsenosidimutans gsoil 3054(t). | the gene encoding an α-l: -arabinofuranosidase that could biotransform ginsenoside rc {3-o-[β-d: -glucopyranosyl-(1-2)-β-d: -glucopyranosyl]-20-o-[α-l: -arabinofuranosyl-(1-6)-β-d: -glucopyranosyl]-20(s)-protopanaxadiol} to ginsenoside rd {3-o-[β-d: -glucopyranosyl-(1-2)-β-d: -glucopyranosyl]-20-o-β-d: -glucopyranosyl-20(s)-protopanaxadiol} was cloned from a soil bacterium, rhodanobacter ginsenosidimutans strain gsoil 3054(t), and the recombinant enzyme was characterized. the enzyme (abfa) hydro ... | 2011 | 22159603 |
| denitrifying bacteria from the genus rhodanobacter dominate bacterial communities in the highly contaminated subsurface of a nuclear legacy waste site. | the effect of long-term mixed-waste contamination, particularly uranium and nitrate, on the microbial community in the terrestrial subsurface was investigated at the field scale at the oak ridge integrated field research challenge (orifrc) site in oak ridge, tn. the abundance, community composition, and distribution of groundwater microorganisms were examined across the site during two seasonal sampling events. at representative locations, subsurface sediment was also examined from two boreholes ... | 2011 | 22179233 |
| Description of Rhodanobacter denitrificans sp. nov., isolated from nitrate-rich zones of a contaminated aquifer. | Bacterial strains 2APBS1T and 116-2 were isolated from the subsurface of a nuclear legacy waste site where sediments are co-contaminated with large amounts of acidity, nitrate, metal radionuclides and other heavy metals. A combination of physiological and genetic assays indicated that these strains represent the first members of the Rhodanobacter genus shown to be capable of complete denitrification. Cells of strain 2APBS1T and 116-2 were Gram negative, non-spore-forming, rods, 3-5 micro;m long ... | 2011 | 22140175 |
| rhodanobacter caeni sp. nov., a denitrifying bacterium isolated from sludge in a sewage disposal plant. | gram-staining-negative, motile bacteria, designated as mj01t and mj14 were isolated from a sludge sample of the daejeon sewage disposal plant in korea and were characterized using a polyphasic taxonomic approach. comparative 16s rrna gene sequence analysis showed that strains mj01t and mj14 belonged to the genus rhodanobacter in the family xanthomonadaceae of the gammaproteobacteria. according to 16s rrna gene sequences strains mj01t and mj14 were indistinguishable and showed 98.8% - 96.4% simil ... | 2012 | 22228656 |