| ultrastructure of the peripheral zone of a glugea-induced xenoma. | the glugea stephani-induced xenoma in the winter flounder, pseudopleuronectes americanus, is a large spherical host-parasite complex, up to 4.0 mm in diameter, with the host and parasite components of the xenoma being most active in the peripheral zone. the xenoma has an extensive periodic acid-silver methenamine-positive surface coat covering the plasma membrane. the surface of this membrane is amplified by the presence of numerous folds and fine tubular extensions. the peripheral zone of the x ... | 1976 | 933079 |
| laboratory and field studies on glugea stephani (hagenmuller), a microsporidan parasite of pleuronectid flatfishes. | the microsporidan glugea stephani is a common parasite of juvenile english sole (parophrys vetulus) in yaquina bay, oregon. field observations indicated that fish became infected only in the upper estuary where summer temperatures were above 15c and the incidence of infection reached 79.8% in the late fall. laboratory infections developed and parasite growth occurred only at or above 15c. the parasite was successfully transmitted to juvenile english sole by brine shrimp (artemia salina) and amph ... | 1976 | 1271307 |
| the effect of the microsporida glugea stephani on the immunoglobulin levels of juvenile and adult winter flounder (pseudopleuronectes americanus). | the microsporida glugea stephani is an intracellular parasite that causes a decline in the serum igm levels of both adult and juvenile winter flounder, shortly after introduction. this decline was noted in fish injected with spores or infected via an intermediary vector. the parasite also suppresses the humoral response to other antigens such as horse red blood cells. the suppression of the response to the antigen however, is related directly to the exposure time with regard to the kinetic state ... | 1989 | 2504626 |
| the immune response of a marine teleost, pseudopleuronectes americanus, (winter flounder) to the protozoan parasite glugea stephani. | g. stephani is an intracellular cyst-forming microsporidan parasite that is found in the intestine of winter flounder (wf) pseudopleuronectes americanus. no detectable humoral response was seen in parasitized fish or in fish injected with either spores or spore homogenate from this parasite. quantification of total immunoglobulin (ig) levels showed a decrease in ig levels rather than enhancement, 21 days after intramuscular (im) injections of spores (3 x 10(6)/ml). when a second injection of spo ... | 1986 | 3094232 |
| immunoglobulin levels of the winter flounder (pseudopleuronectes americanus) and the summer flounder (paralichthys dentatus) injected with the microsporidan parasite glugea stephani. | the microsporidan parasite g. stephani, has been found in the intestine of the american winter flounder pseudopleuronectes americanus. a quantitative study of total serum immunoglobulin (ig) levels has shown that an injection of this parasitic spore causes a decrease in serum igm levels. the level of suppression seems to be related to the number of spores injected into the host, as well as the frequency of injections administered. as the dosage of spores injected into the winter flounder increas ... | 1986 | 3095157 |
| birefringent spores differentiate encephalitozoon and other microsporidia from coccidia. | tissue sections containing protozoa with birefringent spores indicate an infection by microsporidia. hematoxylin and eosin (he) does not affect spore birefringence, but some special stains (goodpasture, brown and brenn, or gram) obscure it. encephalitozoon cuniculi from an infected puppy, glugea stephani from the winter flounder pseudopleuronectes americanus, and plistophora sp. from the japanese eel anguilla japonica all have birefringent spores. encephalitozoon was studied first and then the t ... | 1988 | 3134761 |
| [evolution of xenomas and the double role of polynuclear neutrophils in the microsporodiosis of glugea stephani (hagenmuller, 1899) in the flounder platichthys flesus (linne, 1758)]. | glugea stephani forms xenomas in the intestinal wall and in several organs of the flounder platichthys flesus in the languedoc's ponds. four characteristic stages are recognised in the development of these xenomas: reinforcement of the wall and maintenance of the host-parasite equilibrium, inflammation, granulomaformation, cicatrisation. the role of the polymorph neutrophiles is essential, first because it harbours the sporoplasm and then because it contributes to the destruction of the spores. ... | 1985 | 4083678 |
| effects of low temperature on the development of the microsporidan glugea stephani in english sole (parophrys vetulus). | glugea stephani requires temperatures above 15 c for development in juvenile pleuronectid flatfishes in yaquina bay, oregon. the effect of low temperature (10 c) on the development of recently established parasites was tested experimentally in juvenile english sole (parophrys vetulus). low temperature arrested parasite development, but did not kill the protozoan which resumed development on return to 19-20 c after as long as 42 days at 10 c. no parasites detectable with the light microscope were ... | 1981 | 6802989 |
| enzyme histochemical identification of the golgi apparatus in the microsporidian, glugea stephani. | | 1994 | 7804262 |
| comparative study of microsporidian spores by flow cytometric analysis. | spore suspensions of microsporidian parasites of fish (microsporidium ovoideum, glugea stephani, glugea atherinae and spraguea lophii) have been analyzed by flow cytometry. spore nuclei were dyed either by propidium iodide or bis-benzimide (hoechst 33342). by observation of forward light scatter and fluorescence the four species could be distinguished and the mono- and diplokaryotic populations of s. lophii identified. staining of dna by bis-benzimide was better and easier than propidium iodide. ... | 1994 | 8049684 |
| longevity and effects of temperature on the viability and polar-tube extrusion of spores of glugea stephani, a microsporidian parasite of commercial flatfish. | food contamination with respect to microsporidiosis in humans (associated with acquired immunodeficiency syndrome) and in marine fish farming deserves particular attention. for the first time, a study on the longevity and resistance to both heat and freezing of the spores of a vertebrate microsporidian, glugea stephani, parasitizing a commercial flatfish was carried out. as judged on the basis of determinations of the extrusion rate, the resistance of the spores to temperature stress was remarka ... | 1996 | 8801551 |
| polar tube formation and nucleoside diphosphatase activity in the microsporidian, glugea stephani. | | 1996 | 8822890 |
| identification and phylogenetic relationships of microsporidia by riboprinting. | the ssurdna and the its of different microsporidia from eight fishes, four insects and a shrimp were amplified and digested with restriction enzymes. the generated riboprints suggest a close evolutionary relationship between glugea americanus and spraguea lophii suggesting that glugea americanus should be renamed spraguea americanus and that the tissue infected and host origin should be considered of greater taxonomic importance for defining a genus than previously considered. phylogenetic analy ... | 1997 | 9435126 |
| the parasite community infecting flounders, platichthys flesus, in the tidal thames. | the composition of the parasite fauna of the flounder, platichthys flesus, retrieved from two locations in the tidal thames is described in detail for the first time. the combined parasite species list of the flounders from lots road in the upper tideway and west thurrock in the middle tideway consisted of one protozoan (glugea stephani), one monogenean (gyrodactylus sp.), four larval digeneans (cryptocotyle concava, timoniella imbutiforme, t. praeterita, and labratrema minimus), five adult dige ... | 1999 | 10526412 |
| effect, distribution, and prevalence of glugea stephani (microspora) in winter flounder (pleuronectes americanus) living near two pulp and paper mills in newfoundland. | a study was conducted to determine the effects, geographical distribution, and prevalence of a microsporan parasite, glugea stephani, in winter flounder (pleuronectes americanus) in newfoundland. fish were captured by scuba divers in several coastal areas, including 2 embayments where pulp and paper mill effluent was discharged, as well as a number of pristine sites. fish health was assessed by comparing histological profiles, condition factors (k), organosomatic indices and blood values between ... | 2004 | 15165042 |
| temporal changes in the prevalence of parasites in two oregon estuary-dwelling fishes. | the parasite faunas of juvenile english sole (parophrys vetulus) in 1971-1972 and staghorn sculpin (leptocottus armatus) in 1971 from yaquina bay, oregon, were compared with faunas found in the same estuary in 1997-2000 (english sole) and 1999-2000 (staghorn sculpin). the 7 most commonly occurring parasites in 1971 were compared with the same species observed during the same month and sampling sites in 1997-2000. multivariate community analysis of juvenile english sole parasites supported the su ... | 2004 | 15270100 |
| validation of a quantitative pcr diagnostic method for detection of the microsporidian ovipleistophora ovariae in the cyprinid fish notemigonus crysoleucas. | microsporidian parasites are easily detected by light microscopy when infections are heavy and spores are present. however, early infections without spores, or light infections with low numbers of spores, are easily missed. this limitation has made it difficult to conduct investigations into microsporidian prevalence and transmission. in this study, we developed a quantitative taqman polymerase chain reaction assay to assess the presence of ovipleistophora ovariae in the tissues of the cyprinid ... | 2007 | 17803107 |