conformational analysis of the exopolysaccharide from burkholderia caribensis strain mwap71: impact on the interaction with soils.the strain mwap71 of burkholderia caribensis produces a branched charged exopolysaccharide (eps) that is responsible for soil aggregation. understanding the conformational properties of the isolated polysaccharide is a prerequisite for proper investigation of the interactions between the polysaccharide and the soil at the atomic level. the aim of this study is first to have an overall view of the flexibility of the backbone and then to ascertain the role played by side groups in the conformation ...200312879494
proof that burkholderia strains form effective symbioses with legumes: a study of novel mimosa-nodulating strains from south america.twenty mimosa-nodulating bacterial strains from brazil and venezuela, together with eight reference mimosa-nodulating rhizobial strains and two other beta-rhizobial strains, were examined by amplified rrna gene restriction analysis. they fell into 16 patterns and formed a single cluster together with the known beta-rhizobia, burkholderia caribensis, burkholderia phymatum, and burkholderia tuberum. the 16s rrna gene sequences of 15 of the 20 strains were determined, and all were shown to belong t ...200516269788
solution conformation and dynamics of exopolysaccharides from burkholderia species.exopolysaccharides (epss) from the burkholderia genus are proposed to be involved in pathological conditions in humans, such as cystic fibrosis and septicemia, as well as in the stability of soil aggregates. hence, considering that the conformational and dynamic aspects of such epss may influence their biological activity, the current work employs a series of molecular dynamics simulations on di-, oligo-, and polysaccharide fragments of three epss, from burkholderia caribensis, burkholderia cepa ...201020633874
burkholderia terrae sp. nov., isolated from a forest soil.a gram-negative, slightly curved rod-shaped bacterium, designated strain kmy02t, was isolated from a forest soil in daejeon, south korea. on the basis of 16s rrna gene sequence similarity, strain kmy02t was shown to belong to the family burkholderiaceae of the betaproteobacteria, and to be related most closely to burkholderia hospita lmg 20598t (98.7%), burkholderia caribensis lmg 18531t (98.0%) and burkholderia phymatum lmg 21445t (97.4%). its phylogenetic distance from all recognized species w ...200616449457
legume symbiotic nitrogen fixation by beta-proteobacteria is widespread in nature.following the initial discovery of two legume-nodulating burkholderia strains (l. moulin, a. munive, b. dreyfus, and c. boivin-masson, nature 411:948-950, 2001), we identified as nitrogen-fixing legume symbionts at least 50 different strains of burkholderia caribensis and ralstonia taiwanensis, all belonging to the beta-subclass of proteobacteria, thus extending the phylogenetic diversity of the rhizobia. r. taiwanensis was found to represent 93% of the mimosa isolates in taiwan, indicating that ...200314645288
structural analysis of the exopolysaccharide from burkholderia caribensis strain mwap71.burkholderia caribensis strain mwap71 was isolated from rhizosphere soil microaggregates in martinique. the extracellular polysaccharide produced by this strain was found to be composed of d-glucose (d-glc), 6-deoxy-l-talose (l-6dtal), 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), and an o-acetyl group in a molar ratio of 2:1:1:1. the primary structure of the polysaccharide was shown by sugar analysis, electrospray mass spectrometry, partial acid hydrolysis and 1-d and 2-d nmr spectroscopy to consi ...200111502268
burkholderia fungorum sp. nov. and burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples.a polyphasic taxonomic study that included dna-dna hybridizations, dna base ratio determinations, 16s rdna sequence analyses, whole-cell protein and fatty acid analyses and an extensive biochemical characterization was performed on 16 strains isolated from the environment, animals and human clinical samples. the isolates belonged to the genus burkholderia, were phylogenetically closely related to burkholderia graminis, burkholderia caribensis and burkholderia phenazinium and had g+c contents bet ...200111411678
burkholderia caribensis sp. nov., an exopolysaccharide-producing bacterium isolated from vertisol microaggregates in martinique.twenty-one exopolysaccharide-producing strains were isolated from the 5-20 microns fraction of a vertisol in the south-east of the island of martinique in the french west indies. although these strains were phenotypically identified as burkholderia cepacia or as burkholderia glathei using biolog microplates, they did not cluster genotypically by amplified rdna restriction analysis (ardra) with any described burkholderia species. a phylogenetic analysis revealed that the rrs (16s rdna) sequences ...199910319504
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