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neopolystoma liewi sp. n. (monogenea: polystomatidae) from the eye of the malayan box turtle (cuora amboinensis).neopolystoma liewi sp. n. is described from the conjunctival cavity of the malayan box turtle cuora amboinensis (daudin, 1802), in peninsular malaysia. this is the first record of neopolystoma in malaysia and the fourth polystomatid species described from c. amboinensis. of the 27 malayan box turtles examined, 8 were found to be infected. a maximum of 2 parasites per eye and 4 individuals per host was recorded. n. liewi sp. n. differs from all other members of the genus by possessing few and sho ...200010833010
analysis of prey capture and food transport kinematics in two asian box turtles, cuora amboinensis and cuora flavomarginata (chelonia, geoemydidae), with emphasis on terrestrial feeding patterns.this study examines the kinematics and morphology of the feeding apparatus of two geoemydid chelonians, the malayan (amboina) box turtle (cuora amboinensis) and the yellow-margined box turtle (cuora flavomarginata). both species are able to feed on land as well as in water. feeding patterns were analysed by high-speed cinematography. the main focus of the present study is on the terrestrial feeding strategies in both asian box turtles, because feeding on land has probably evolved de novo within ...200919010648
the distal forelimb musculature in aquatic and terrestrial turtles: phylogeny or environmental constraints?we compared the muscular anatomy of the distal front limb in terrestrial and aquatic chelonians to test whether observed differences between the two groups are associated with their divergent lifestyles and locomotor modes. given the different use of the forelimb in the two environments (body support and propulsion on land vs. mainly propulsion in water) we expected that: (1) aquatic and terrestrial turtles would show differences in their muscular anatomy, with aquatic species having more indivi ...200819172731
lab-on-a-chip-based pcr-rflp assay for the detection of malayan box turtle (cuora amboinensis) in the food chain and traditional chinese medicines.the malayan box turtle (cuora amboinensis) (mbt) is a vulnerable and protected turtle species, but it is a lucrative item in the illegal wildlife trade because of its great appeal as an exotic food item and in traditional medicine. although several polymerase chain reaction (pcr) assays to identify mbt by various routes have been documented, their applicability for forensic authentication remains inconclusive due to the long length of the amplicon targets, which are easily broken down by natural ...201627716792
a new species of opisthioglyphe (trematoda: telorchiidae) from gall bladder of turtles in malaysia.opisthioglyphe sharmai n. sp. is described from the gall bladder of the malayan box turtle, cuora amboinensis, and the black marsh turtle, siebenrockiella crassicollis, in malaysia. the new species is morphologically similar to opisthioglyphe ranae and some other members of the genus parasitic in amphibians and reptiles. opisthioglyphe sharmai n. sp. is easily differentiated from all other members of the genus by the cirrus sac extending posterior to the ventral sucker, while in all previously k ...201222263805
a new species of blood fluke (digenea: spirorchiidae) from the malayan box turtle, cuora amboinensis (cryptodira: geomydidae) in thailand.spirhapalum siamensis n. sp. obtained from the heart of the malayan box turtle, cuora amboinensis, from western thailand is described. this blood fluke differs from the 2 previously known species of spirhapalum in its larger overall body size, constricted mid-body, greater number of pre-ovarian testes, ceca that loop anteriorly from the cecal bifurcation before turning to the posterior, and the relatively smaller size of its ventral sucker. a phylogenetic analysis using molecular characters plac ...200919093710
duplex real-time pcr assay using sybr green to detect and quantify malayan box turtle (cuora amboinensis) materials in meatballs, burgers, frankfurters and traditional chinese herbal jelly powder.the malayan box turtle (cuora amboinensis) (mbt) is a vulnerable and protected species widely used in exotic foods and traditional medicines. currently available polymerase chain reaction (pcr) assays to identify mbt lack automation and involve long targets which break down in processed or denatured tissue. this sybr green duplex real-time pcr assay has addressed this research gap for the first time through the combination of 120- and 141-bp targets from mbt and eukaryotes for the quantitative d ...201627643977
new genus of blood fluke (digenea: schistosomatoidea) from malaysian freshwater turtles (geoemydidae) and its phylogenetic position within schistosomatoidea.:  baracktrema obamai n. gen., n. sp. infects the lung of geoemydid turtles (black marsh turtle, siebenrockiella crassicollis [type host] and southeast asian box turtle, cuora amboinensis ) in the malaysian states of perak, perlis, and selangor. baracktrema and unicaecum stunkard, 1925 are the only accepted turtle blood fluke genera having the combination of a single cecum, single testis, oviducal seminal receptacle, and uterine pouch. baracktrema differs from unicaecum by having a thread-like b ...201627042972
a suitable method to detect potential fraud of bringing malayan box turtle (cuora amboinensis) meat into the food chain.malayan box turtle (cuora amboinensis) has been a wildlife-protected vulnerable turtle species in malaysia since 2005. however, because of its purported usage in traditional medicine, tonic foods and feeds, clandestine black market trade is rampant. several polymerase chain reaction (pcr) assays for the taxonomic detection and classification of turtle species have been proposed. these assays are based on long-length target amplicons which are assumed to break down under compromised states and, h ...201526062948
hematology of the pascagoula map turtle (graptemys gibbonsi) and the southeast asian box turtle (cuora amboinensis).turtle populations are decreasing dramatically due to habitat loss and collection for the food and pet market. this study sought to determine hematologic values in two species of turtles to help assess health status of captive and wild populations. blood samples were collected from 12 individuals of the pascagoula map turtle (graptemys gibbonsi) and seven individuals of the southeast asian box turtle (cuora amboinensis) from the savannah river ecology laboratory (south carolina, usa). the hemato ...200818817012
microanatomy of the palatal mucosa of the semiaquatic malayan box turtle, cuora amboinensis, and functional implications.scanning electron microscopy (sem) revealed that the palate of cuora amboinensis has a flat surface with keratinized and non-keratinized regions. keratinization is reflected in disc-shaped keratinized dead cells with rough microplicae on the surface, and is concentrated close to the rhamphotheca. the surface of the non-keratinized hexagonal epithelial cells is dotted with microvilli and sometimes with cilia. taste buds are present both in lightly keratinized and non-keratinized regions and exhib ...200818449902
redescription of serpinema octorugatum (baylis, 1933) nematoda: camallanidae from the malayan box turtle cuora amboinensis daudin chelonia: bataguridae.we redescribe the camallanid nematode serpinema octorugatum (baylis, 1933) from the box turtle cuora amboinensis (daudin) collected in malaysia. in this redescription, we amend the original description by noting that there are only four cephalic papillae and that there are five pairs of post-anal papillae, and propose that the name of this species be corrected from s. octorugatus to s. octorugatum. additionally, we removed the tissues overlying the buccal capsule and have used sem studies to sho ...200212378130
[identification of cuora amboinensis and c. flavomarginata and historical studies on c. flavomarginata]. 19852934166
stunkardia minuta sp. n. (trematoda: paramphistomidae) from the malayan box-tortoise cuora amboinensis.stunkardia minuta sp. n. was recovered from the small intestine and rectum of 5 box-tortoises (cuora amboinensis) in malaysia. the average body size (l x w) is 11-42 x 2-35 mm; oral sucker 1-51 x 1.02; oral pouches 0-21 x 0.18; ventral sucker 1-67 x 1-43; oesophageal bulb 0-54 x 0-54; ant. testis 0-95 x 0-98; post. testis 0-94 x 0.94; seminal vesicle 0-82 x 0-24; ootype 0-21 x 0-41; ovary 0-41 x 0-34; and egg 121 x 83 micrometer. although morphologically similar to s. dilymphosa, s. minuta is di ...1977886177
gut bacteria of animals living in polluted environments exhibit broad-spectrum antibacterial activities.infectious diseases, in particular bacterial infections, are the leading cause of morbidity and mortality posing a global threat to human health. the emergence of antibiotic resistance has exacerbated the problem further. hence, there is a need to search for novel sources of antibacterials. herein, we explored gut bacteria of a variety of animals living in polluted environments for their antibacterial properties against multi-drug resistant pathogenic bacteria. a variety of species were procured ...202032124096
redescription of the siamese shield leech placobdelloides siamensis with new host species and geographic range.the siamese shield leech placobdelloides siamensis (oka, 1917) sawyer, 1986 (euhirudinea: glossiphoniidae) was collected from five new host species, southeastern asian box turtle (cuora amboinensis), yellow-headed temple turtle (heosemys annandalii), malayan snail-eating turtle (malayemys macrocephala), mekong snail-eating turtle (m. subtrijuga), and khorat snail-eating turtle (m. khoratensis) and was found for the first time in udon thani, thailand. examination of live leeches provided, for the ...201830474597
non-marine turtle plays important functional roles in indonesian ecosystems.the southeast asian box turtle (cuora amboinensis) is numerically the most important turtle exported from indonesia. listed as vulnerable by the iucn, this turtle is heavily harvested and exported for food and traditional medicine in china and for the pet trade primarily in the united states, europe, and japan. despite its significance in global markets, relatively little is known about the species' ecology or importance to ecosystems. we conducted our research in a national park in sulawesi, in ...202033005334
gut bacteria of cuora amboinensis (turtle) produce broad-spectrum antibacterial molecules.antimicrobial resistance is a major threat to human health, hence there is an urgent need to discover antibacterial molecule(s). previously, we hypothesized that microbial gut flora of animals are a potential source of antibacterial molecules. among various animals, cuora amboinensis (turtle) represents an important reptile species living in diverse ecological environments and feed on organic waste and terrestrial organisms and have been used in folk medicine. the purpose of this study was to mi ...201931740685
graphene oxide and gold nanoparticle based dual platform with short dna probe for the pcr free dna biosensing using surface-enhanced raman scattering.surface-enhanced raman scattering (sers) based dna biosensors have considered as excellent, fast and ultrasensitive sensing technique which relies on the fingerprinting ability to produce molecule specific distinct spectra. unlike conventional fluorescence based strategies sers provides narrow spectral bandwidths, fluorescence quenching and multiplexing ability, and fitting attribute with short length probe dna sequences. herein, we report a novel and pcr free sers based dna detection strategy i ...201930844598
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