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a pcr-based marker system monitoring cms-(s), cms-(t) and (n)-cytoplasm in the onion ( allium cepa l.).the chimerical mitochondrial cms(1)-specific sequence in chives ( allium schoenoprasum) was used to develop a pcr-marker that distinguishes both male-sterility inducing cytoplasms, cms-(s) and cms-(t), from the normal cytoplasm in onion ( allium cepa). in combination with a previously described marker for cms-(s), which anchors in the upstream region of the mitochondrial gene cob, all of the three known cytoplasms in the onion are distinguishable. the pcr-marker system was tested in 361 onion pl ...200312835941
bioavailability of selenium from selenium-enriched green onions (allium fistulosum) and chives (allium schoenoprasum) after 'in vitro' gastrointestinal digestion.three sample preparation methods--proteolysis to determine the initial species distribution, and an in vitro gastric and gastrointestinal digestion to assess the bioavailability of selenium--were applied to extract the selenium from selenized green onion and chive samples. ion exchange chromatography was coupled to a high-performance liquid chromatography-icp-ms system to analyze the selenium species of allium samples. the difference in the selenium accumulation capability of green onions and ch ...200717566890
[conidiobolus coronatus isolation from a pest aphids of chives (allium schoenoprasum l.)].conidiobolus coronatus (entomophthorales), a pathogen of human and some insects, was isolated from dead specimens of the aphid neotoxoptera formosana present on chives leaves. c. coronatus infected aphids could pose a threat to growers health. pest control strategies of n. formosana may also contribute to reduce the human infection risk by c. coronatus.200818785794
diallyl sulfide content and antimicrobial activity against food-borne pathogenic bacteria of chives (allium schoenoprasum).chives, a member of the alliaceae family, have been used in food and medicine in thailand for a long time. diallyl sulfides (diallyl monosulfide, dially disulfide, diallyl trisulfide, and diallyl tetrasulfide) are believed to be responsible for the antimicrobial activity of plants in this family. in this study, chive oil was examined for its diallyl sulfide content and its antimicrobial activity against some strains of food-borne pathogenic bacteria. chive oil had a very low concentration of dia ...200818997412
phenolic compounds from allium schoenoprasum, tragopogon pratensis and rumex acetosa and their antiproliferative effects.experimental studies have shown that phenolic compounds have antiproliferative and tumour arresting effects. the aim of this original study was to investigate the content of phenolic compounds (phc) in flowers of allium schoenoprasum (chive), tragopogon pratensis (meadow salsify) and rumex acetosa (common sorrel) and their effect on proliferation of hacat cells. antiproliferative effects were evaluated in vitro using the following concentrations of phenolic compounds in cultivation medium: 100, ...201122051932
specificity patterns indicate that auxin exporters and receptors are the same proteins.a study of transport and action of synthetic auxin analogues can help to identify transporters and receptors of this plant hormone. both aspects--transportability and action on growth--were tested with 2-naphthoxyacetic acid (2-noa) and compared across several plant species. 2-noa stimulates elongation effectively at low concentrations in petioles of the gymnosperm ginkgo biloba l., in hypocotyls or internodes of the dicot legumes, mung bean (vigna mungo l.) and pea (pisum sativum l.), in cotyle ...200515666213
genotoxicity and cytotoxicity assay of water sampled from the underground nuclear explosion site in the north of the perm region (russia).the results of our study revealed a local biologically relevant surface water contamination in the radionuclide anomaly in the north of russia (perm region) by means of allium schoenoprasum l. anaphase-telophase chromosome aberration assay. this radionuclide anomaly was formed in 1971 as a result of an underground nuclear explosion with soil excavation. specific activities of main dose-forming radionuclides in all examined reservoirs are below intervention levels officially adopted in russia for ...200515653187
slow-speed freezing of chemically unfixed biological tissues and long-term storage of frozen samples for cryoscanning electron microscopy.we describe a procedure in which plant tissue as well as a yeast culture on agar are frozen with slow cooling rates for observation of surface structures in a cryoscanning electron microscope. a system is also presented for long-term storage of frozen specimens under liquid nitrogen, in which the material is maintained for direct observation. some small tools are described, which are essential for making preparations using slow-speed freezing and for the storage of prepared samples. three exampl ...19968652884
chemical composition, antibacterial and antioxidant activities of six essentials oils from the alliaceae family.six essential oils (eos) from the alliaceae family, namely garlic (allium sativum), onion (allium cepa), leek (allium porrum), chinese chive (allium tuberosum), shallot (allium ascalonicum) and chive (allium schoenoprasum) were characterized by gc and gc-ms and evaluated for their functional food properties. antibacterial properties were tested on five food-borne pathogens: two gram-positive staphylococcus aureus (atcc 25923), listeria monocytogenes (atcc 19115) and three gram-negative salmonell ...201425470273
in vitro study of antitubercular substances from allium species. i. allium schoenoprasum. ii. allium cepa. 195524543342
evolution of 5s rdna units and their chromosomal localization in allium cepa and allium schoenoprasum revealed by microdissection and fish.allium cepa and allium schoenoprasum each possess 5s rdna units of two different sizes. the evolution of the two repeat units and their chromosomal localization were investigated. a. cepa has 5s rdna loci in the proximal and distal regions of the short arm of chromosome 7. when the proximal and distal segments of the short arm of chromosome 7 were microdissected separately, and used as templates for pcr, the short and long 5s rdna fragments were amplified predominantly from the proximal and dist ...200212582516
engineered nickel oxide nanoparticle causes substantial physicochemical perturbation in plants.concentration of engineered nickel oxide nanoparticle (nio-np) in nature is on the rise, owing to large scale industrial uses, which have accreted the scope of its exposure to plants, the primary producers of the ecosystem. though an essential micronutrient for the animal system, supported by numerous studies confirming its toxicity at higher dosages, nickel oxide is graded as a human carcinogen by who. a few studies do depict toxicity and bioaccumulation of nickel in plants; however, interactio ...201729167790
impact of air frying on cholesterol and fatty acids oxidation in sardines: protective effects of aromatic herbs.the high temperatures used to fry fish may induce thermo-oxidation of cholesterol, forming cholesterol oxidation products (cops). cops have been associated to coronary heart diseases, atherosclerosis, and other chronic diseases. air fryers are an alternative thermal process technology to fry foods without oil, and are considered a healthier cooking method. this study is the 1st to evaluate the formation of cops and the degradation of polyunsaturated fatty acids (pufas) in air-fried sardine fille ...201729125626
allium schoenoprasum l.: a review of phytochemistry, pharmacology and future directions.allium schoenoprasum l. (family amaryllidaceae), commonly known as chives has great culinary value besides being used as ethnomedicine. this review emphasises on phytochemistry and pharmacological activities of a. schoenoprasum, and discusses the future opportunities for systematic investigations. scientific evaluation of chives validates its traditional claims and demonstrates diverse pharmacological potential including an anti-inflammatory, anticancer, antioxidant, anthelmintic and antihyperte ...201728826254
vascular plant biodiversity of the lower coppermine river valley and vicinity (nunavut, canada): an annotated checklist of an arctic flora.the coppermine river in western nunavut is one of canada's great arctic rivers, yet its vascular plant flora is poorly known. here, we report the results of a floristic inventory of the lower coppermine river valley and vicinity, including kugluk (bloody falls) territorial park and the hamlet of kugluktuk. the study area is approximately 1,200 km2, extending from the forest-tundra south of the treeline to the arctic coast. vascular plant floristic data are based on a review of all previous colle ...201728194307
[cytogenetic effects in allium schoenoprasum growing on the anthropogenically contaminated soil].cytogenetic effects in allium schoenoprasum meristematic root tip cells grown for a year on the territory contaminated with 235u, 238u and 232th decay series radionuclides, heavy metals and as were studied. the area is characterized with different concentrations of chemical compounds in soil affecting a toxic element migration in biocoenosis. analysis of the chromosome aberration spectrum showed an ambiguous cell response to soil contamination. within the weighted absorbed dose range up to 1.2 g ...201625962271
anti-inflammatory effects of allium schoenoprasum l. leaves.allium schoenoprasum has antimicrobial and antifungal properties and is used to relieve pain from sunburn and sore throat. the aim of the present study was to evaluate the anti-inflammatory effects of the extracts from a. schoenoprasum leaves. a 1:1 (w:v) extract was prepared by a modified squibb repercolation method. the total phenolic content of 68.5±2 g gallic acid aquivalent (gae)/g plant was determined using the folin-ciocalteu method. the in vitro antioxidant activity was determined using ...201424781739
polyphenolic extracts of edible flowers incorporated onto atelocollagen matrices and their effect on cell viability.the phenolic extract of chives flowers (allium schoenoprasum, liliaceae), introduced sage (salvia pratensis, lamiaceae), european elderberry (sambucus nigra, caprifoliaceae) and common dandelion (taraxacum officinale, asteraceae) were characterised by high performance liquid chromatography and incorporated in different concentrations onto atelocollagen thin films. in order to assess the biological impact of these phenolic compounds on cell viability, human immortalised non-tumorigenic keratinocy ...201324177700
structure and cytotoxicity of steroidal glycosides from allium schoenoprasum.a phytochemical analysis of the whole plant of allium schoenoprasum, has led to the isolation of four spirostane-type glycosides (1-4), and four known steroidal saponins. their structures were elucidated mainly by 2d nmr spectroscopic analysis and mass spectrometry as (20s,25s)-spirost-5-en-3β,12β,21-triol 3-o-α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranoside (1), (20s,25s)-spirost-5-en-3β,11α,21-triol 3-o-α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranoside (2), laxogenin 3-o-α-l-rhamnopyranosyl-(1→2)-[β- ...201323357597
covalent anthocyanin-flavonol complexes from the violet-blue flowers of allium 'blue perfume'.three covalent anthocyanin-flavonol complexes (pigments 1-3) were extracted from the violet-blue flower of allium 'blue perfume' with 5% acetic acid-meoh solution, in which pigment 1 was the dominant pigment. these three pigments are based on delphinidin 3-glucoside as their deacylanthocyanin and were acylated with malonyl kaempferol 3-sophoroside-7-glucosiduronic acid or malonyl-kaempferol 3-p-coumaroyl-tetraglycoside-7-glucosiduronic acid in addition to acylation with acetic acid. by spectrosc ...201222704652
comparative study on allium schoenoprasum cultivated plant and allium schoenoprasum tissue culture organs antioxidant status.this study was designed to examine allium schoenoprasum tissue culture organs antioxidant and scavenging activity and to make a comparison between allium schoenoprasum cultivated plant and allium schoenoprasum tissue culture organs antioxidant activity. this study reports the results on the root, stalk and leaf antioxidant enzyme activities (superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidase), reduced glutathione quantity, flavonoids and soluble protein contents and q ...201121438046
a molecular phylogeny of the wild onions (allium; alliaceae) with a focus on the western north american center of diversity.nuclear ribosomal dna (its and ets) sequences from 39 native californian (usa) allium species and congeners were combined with 154 its sequences available on genbank to develop a global allium phylogeny with the simultaneous goals of investigating the evolutionary history (monophyly) of allium in the californian center of diversity and exploring patterns of adaptation to serpentine soils. phylogenies constructed with its alone or its in combination with ets provided sufficient resolution for inv ...200818226928
diffusion correlation nmr spectroscopic study of anisotropic diffusion of water in plant tissues.the anisotropic diffusion of water in chive (allium schoenoprasum) tissues has been investigated using two-dimensional nuclear magnetic resonance methods: diffusion-diffusion correlation spectroscopy and diffusion-relaxation correlation spectroscopy. corresponding one-dimensional t2 and diffusion measurements confirm independently the results of the two-dimensional investigations. in particular the diffusion-diffusion correlation spectroscopy method proves to be very powerful in resolving the di ...200516100271
cadmium accumulation in allium schoenoprasum l. grown in an aqueous medium.the ability of allium schoenoprasum l. (chives) to accumulate and tolerate cadmium in aqueous hoagland medium at 50microm and 250microm was tested under continuous growth or several successive harvests of shoots. after 28 days of continuous growth, chives accumulated the metal up to 0.2% and 0.5% of its dry weight, when grown in 50microm and 250microm, respectively. in experiments that the leaves were successively harvested every 16 days, there were no obvious stress symptoms after six harvests ...200415504482
allium schoenoprasum l., as a natural antioxidant.the present study investigated the antioxidative properties of the bulb, leaf and stalk of allium schoenoprasum l. activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, glutathione peroxidase), quantities of malonyldialdehyde, superoxide and hydroxyl radicals and reduced glutathione and also the content of total flavonoids, chlorophylls a and b, carotenoids, vitamin c and soluble proteins were determined. the results indicate that extracts from all plant organs exhibited ...200415305309
the fertility restorer genes x and t alter the transcripts of a novel mitochondrial gene implicated in cms1 in chives (allium schoenoprasum l.).a chimeric mitochondrial gene configuration, mainly derived from sequences associated with the essential genes atp9 and atp6, was isolated from the sterility-inducing cytoplasm of the cms1 system in chives (allium schoenoprasum l.). this sequence is not found in four other cytoplasm types from chives; however, two copies are present in the mitochondrial dna of cms1-inducing cytoplasm, whose 5'-sequences are homologous to those of the atp9 gene. we provide evidence to show that one of the two cms ...200414727184
genotoxicity and toxicity assay of water sampled from a radium production industry storage cell territory by means of allium-test.water from natural reservoirs located near the radium production industry storage cell were analyzed using the anaphase-telophase chromosome aberration assay that was carried out on allium schoenoprasum l. meristematic root tip cells. (262)ra, (228)u, (232)th, (210)pb and (210)po concentrations in all samples were found not to exceed the radioactivity concentration guides. the concentrations of 10 heavy metal ions were measured in water samples, but only zn and mn levels exceeded the maximum per ...200312782475
a pcr-marker for the cms(1) inducing cytoplasm in chives derived from recombination events affecting the mitochondrial gene atp9.the complete coding and 3;-flanking region of the mitochondrial gene atp9 of chives ( allium schoenoprasum l.) was determined in order to develop primers that allow the identification of atp9-related sequences in subsequent pcr-amplifications. one of these sequences is of a chimerical nature, consisting of atp9-homologous regions on its end, interrupted by an insertion that is composed of one atp6-homologous part and one part of unknown origin. this pcr-fragment is 762 bp in size and exclusively ...200212582676
covalent anthocyanin-flavonol complexes from flowers of chive, allium schoenoprasum.the structures of eight anthocyanins have been determined in acidified methanolic extract of pale-purple flowers of chive, allium schoenoprasum. four of them have been identified as the anthocyanin-flavonol complexes (cyanidin 3-o-beta-glucosideaii) (kaempferol 3-o-(2-o-beta-glucosylfiii-beta-glucosidefii)-7-o-beta-gl ucosiduronic acidfiv) malonateaiii (aii-6-->aiii-1, fiv-2-->aiii-3), 1, (cyanidin 3-o-(3-o-acetyl-beta-glucosideaii) (kaempferol 3-o-(2-o-beta-glucosylfiii-beta-glucosidefii)-7-o-b ...200010870187
the inheritance of b chromosomes in allium schoenoprasum l.the inheritance of b chromosomes has been investigated. in allium schoenoprasum from the river wye, powys; controlled crosses between plants of known b chromosome constitution were carried out, and the numbers of bs present in the progenies scored. there was considerable heterogeneity in b chromosome transmission rates across the range of b chromosome numbers, through both the male and female lines, and in crosses involving odd and even numbers of bs. the mean b number of the progeny was lower t ...19968785610
rdna site number polymorphism and nor inactivation in natural populations of allium schoenoprasum.nucleolar-organiser activity has been studied by silver staining and by in situ hybridization with an rdna probe in two populations of allium schoenoprasum. one population is monomorphic with nors and rdna sites terminal on the short arm of pair 8 in all individuals. the other populations is monomorphic for pair 8 nors but is also polymorphic for nors on the long arm of pair 7. all plants in this population carry ribosomal cistrons on both chromosomes of pair 7 but 0, 1 or 2 of these sites can b ...19947729698
molecular analysis of cytoplasmic male sterility in chives (allium schoenoprasum l.).the mitochondria of chive plants with normal n or male-sterile s cytoplasms have been examined by restriction fragment analysis and southern hybridizations of mitochondrial dna (mtdna) and in organello protein biosynthesis. restriction fragment patterns of the mtdna differed extensively between n-and s-cytoplasms. the percentage of fragments with different mobility varied between 44-48% depending on the restriction enzyme used. in contrast to mtdna, the restriction fragment patterns of the chlor ...199324190316
loss of dna and euchromatin in senescing leaf cells of allium.the leaves of the chive, allium schoenoprasum, have an average life-span of 53 days, then they fade from top to bottom, in the same sequence as cells originated. starting during the adult phase, the amount of dna per nucleus decreases significantly. nuclei of senescent cells exhibit about 15% less dna than nuclei of juvenile cells. electron-microscopic investigations have shown that the diffuse chromatin is lost from the nuclei, followed by shrinkage of the space left. quantitation was achieved ...19827062791
secondary metabolites from endophytic fungus penicillium pinophilum induce ros-mediated apoptosis through mitochondrial pathway in pancreatic cancer cells.the endophytic fungus strain mrcj-326, isolated from allium schoenoprasum, which is also known as snow mountain garlic or kashmiri garlic, was identified as penicillium pinophilum on the basis of morphological characteristics and internal transcribed spacer region nucleotide sequence analysis. the endophytic fungus extract was subjected to 2d-sepbox bioactivity-guided fractionation and purification. the anthraquinone class of the bioactive secondary metabolites were isolated and characterized as ...201626848704
chloroplast phosphofructokinase: i. proof of phosphofructokinase activity in chloroplasts.ammonium sulfate fractionation of an extract from the leaves of spinach (spinacia oleracea l.) produced two fractions of phosphofructokinase activity, the first stimulated by inorganic phosphate and the second inhibited by inorganic phosphate. only the second fraction was obtained from similar treatment of an extract of isolated spinach chloroplasts. the two fractions differed distinctly with respect to kinetics for the substrate fructose 6-phosphate. evidence for these two types of phosphofruct ...197716660078
intercropping garlic plants reduces tetranychus urticae in strawberry crop.the effect of aromatic plants on number of twospotted spider mite (tssm), tetranychus urticae koch, when intercropped with strawberry were assessed in the field. during the first year, chives (allium schoenoprasum l.), coriander (coriandrum sativum l.), fennel (foeniculum vulgare mill.), garlic (allium sativum l.), oregano (origanum vulgare l.), or sweet marjoram (origanum majorana l.) were intercropped with strawberry. assessments were performed on tssm populations by taking six samples from ju ...201627085718
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