| evidence for a group-specific enteroviral antigen(s) recognized by human t cells. | human peripheral blood mononuclear cells from 15 normal, healthy adult volunteers proliferated in vitro against a panel of enteroviral antigens, including coxsackievirus b3, coxsackievirus b2, coxsackievirus b6, coxsackievirus a16, and poliovirus 1. no proliferation against the cardiovirus encephalomyocarditis virus occurred. lymphocytes obtained from cord blood drawn from seven neonates were uniformly nonresponsive to enteroviral antigens. although serum neutralization antibody titers indicated ... | 1990 | 1975596 |
| coxsackievirus group b antibodies in the ventricular fluid of infants with severe anatomic defects in the central nervous system. | ventricular fluids from four of 28 newborn infants who were initially seen with severe congenital anatomic defects in the central nervous system contained neutralizing antibody to at least one serotype of coxsackieviruses group b. two of the four infants with anti-coxsackieviruses group b antibody in the ventricular fluid did not have a detectable level of the same antibody(ies) in their serum. the ventricular fluid of one of the infants had immunoglobulin m neutralizing antibody directed agains ... | 1985 | 2989763 |
| virus validation studies of immunoglobulin preparations. | a validation study of the viral safety of a new polyvalent intravenous immunoglobulin (octagam) according to eu-guideline iii/8115/89-en and the requirements of the federal agency for sera and vaccines in germany was undertaken in may 1994. the following processing steps were analyzed: cohn-oncley fractionation, solvent/detergent (sd) treatment, ph 4 exposure, storage of the final product at low ph and immune neutralisation. | 1996 | 8828948 |
| the complete consensus sequence of coxsackievirus b6 and generation of infectious clones by long rt-pcr. | the full length sequence for the human pathogen coxsackievirus b6 (cvb6, schmitt strain) has been determined. we used long rt-pcr to generate full length dna amplicon of cvb6, and then directly sequenced the amplicons. one-step cloning of the full length amplicon enabled us to obtain an infectious clone of cvb6. rna generated from cvb6 amplicon dna or cvb6 clones, by transcription with t7 rna polymerase, was demonstrated to be infectious upon transfection into hela cells in vitro. the cvb6 genom ... | 1999 | 10500285 |
| nucleotide sequence of the 5'nontranslated and virion polypeptides regions of coxsackievirus b6. | the nucleotide sequence of coxsackievirus b6 (cvb6) has been determined, and the nucleotides encoding the 5' nontranslated region (5' ntr) and virion polypeptides (vp4, 2, 3 and 1) were compared with other serotype cvbs. an unweighted pair-group method analysis (upgma) of phylogenetic trees indicated that the 5' ntr of cvb6 locates on an independent branch from the other cvbs. the tree based on the amino acid sequences showed that cvb6 has close correlation with cvb4 in the vp4 and vp2 regions, ... | 1999 | 10553680 |
| direct and uninterrupted rna amplification of enteroviruses with colorimetric microwell detection. | enteroviruses (ev) cause a broad spectrum of human diseases, of which aseptic meningitis is among the most common and most clinically vexing. while the clinical symptoms of meningitis caused by bacteria, fungi and viruses are similar, the diagnosis, therapy and outcome of disease caused by these agents vary greatly. in order to appropriately manage meningitis patients, rapid and reliable diagnosis of ev meningitis impacts significantly on patient management. | 1995 | 15566806 |
| visualization and detection of infectious coxsackievirus replication using a combined cell culture-molecular beacon assay. | rapid detection of infectious viruses is of central importance for public health risk assessment. by directly visualizing newly synthesized viral rna with molecular beacons (mbs), we have developed a generalized method for the rapid and sensitive detection of infectious viruses from cell culture. an mb, cvb1, specifically targeting the 5' noncoding region of the enterovirus genome was designed and synthesized. introduction of mb cvb1 into permeabilized cells highly infected with coxsackievirus b ... | 2005 | 16332827 |
| treatability of u.s. environmental protection agency contaminant candidate list viruses: removal of coxsackievirus and echovirus using enhanced coagulation. | enhanced coagulation was evaluated for removal efficacy of coxsackievirus and echovirus (contaminant candidate list [ccl] enteroviruses), poliovirus, four potential surrogate bacteriophages, and dissolved organic carbon (doc). viruses and doc were effectively removed using enhanced coagulation, with removals generally improving as dose increased and ph decreased. optimal enhanced coagulation conditions of 40 mg/l fecl3 and ph between 5 and 6.5 resulted in a maximum removal of 3.0 logs of coxsack ... | 2008 | 18853805 |
| visualizing the dynamics of viral replication in living cells via tat peptide delivery of nuclease-resistant molecular beacons. | in this study, we describe the use of nuclease-resistant molecular beacons (mbs) for the real-time detection of coxsackievirus b6 replication in living buffalo green monkey kidney (bgmk) cells via tat peptide delivery. a nuclease-resistant mb containing 2'-o-methyl rna bases with phosphorothioate internucleotide linkages was designed to specifically target an 18-bp 5' noncoding region of the viral genome. for intracellular delivery, a cell-penetrating tat peptide was conjugated to the mb by usin ... | 2008 | 18988730 |
| development and validation of an integrated cell culture-qrtpcr assay for simultaneous quantification of coxsackieviruses, echoviruses, and polioviruses in disinfection studies. | this study demonstrated the applicability of integrated cell culture-quantitative rtpcr (icc-qrtpcr) for the simultaneous quantification of coxsackievirus, echovirus, and poliovirus in disinfection studies. buffalo green monkey cells were inoculated with a 10-fold dilution series of mixed enteroviruses and incubated prior to qrtpcr quantification. optimal assay conditions included three post infection washes and a 24-hour post infection incubation period based on successful differentiation betwe ... | 2010 | 20107264 |
| formononetin inhibits enterovirus 71 replication by regulating cox- 2/pge₂ expression. | the activation of erk, p38 and jnk signal cascade in host cells has been demonstrated to up-regulate of enterovirus 71 (ev71)-induced cyclooxygenase-2 (cox-2)/ prostaglandins e2 (pge₂) expression which is essential for viral replication. so, we want to know whether a compound can inhibit ev71 infection by suppressing cox-2/pge₂ expression. | 2015 | 25890183 |
| efficient replication of recombinant enterovirus b types, carrying different p1 genes in the coxsackievirus b5 replicative backbone. | recombination is an important feature in the evolution of the enterovirus genus. phylogenetic studies of enteroviruses have revealed that the capsid genomic region (p1) is type specific, while the parts of the genome coding for the non-structural proteins (p2-p3) are species specific. hence, the genome may be regarded as consisting of two modules that evolve independently. in this study, it was investigated whether the non-structural coding part of the genome in one type could support replicatio ... | 2015 | 25663145 |