| [construction of microbial metagenomic library and screening of a nematicidal protease gene in greenhouse cucumber soil infested with root-knot nematodes]. | in order to search novel nematicidal protease genes, a metagenomic fosmid library was constructed and screened by uncultured method. | 2010 | 20931876 |
| A Novel N-Acetylglutamate Synthase Architecture Revealed by the Crystal Structure of the Bifunctional Enzyme from Maricaulis maris. | Novel bifunctional N-acetylglutamate synthase/kinases (NAGS/K) that catalyze the first two steps of arginine biosynthesis and are homologous to vertebrate N-acetylglutamate synthase (NAGS), an essential cofactor-producing enzyme in the urea cycle, were identified in Maricaulis maris and several other bacteria. Arginine is an allosteric inhibitor of NAGS but not NAGK activity. The crystal structure of M. maris NAGS/K (mmNAGS/K) at 2.7 Å resolution indicates that it is a tetramer, in contrast to t ... | 2011 | 22174908 |
| microbial community structure of a heavy fuel oil-degrading marine consortium: linking microbial dynamics with polycyclic aromatic hydrocarbon utilization. | a marine microbial consortium obtained from a beach contaminated by the prestige oil spill proved highly efficient in removing the different hydrocarbon families present in this heavy fuel oil. seawater cultures showed a complete removal of all the linear and branched alkanes, an extensive attack on three to five-ring polycyclic aromatic hydrocarbons [pahs; including anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, and benzo(a)pyrene] (30-100%), and a considerable depletion of the ... | 2010 | 20528986 |
| maribaculum marinum gen. nov., sp. nov., isolated from deep seawater. | a taxonomic study was carried out on strain p38(t), which was isolated from an enriched polycyclic aromatic hydrocarbon-degrading consortium from a deep seawater sample collected from the indian ocean. phylogenetic analyses based on 16s rrna gene sequences showed that strain p38(t) formed a distinct evolutionary lineage within the family hyphomonadaceae. strain p38(t) was most closely related to members of the genera hyphomonas (92.3-93.5 % 16s rrna gene sequence similarity), hirschia (88.8 %), ... | 2009 | 19643895 |
| robiginitomaculum antarcticum gen. nov., sp. nov., a member of the family hyphomonadaceae, from antarctic seawater. | a seawater bacterium, designated imcc3195t, was isolated from the antarctic coast. cells of the novel strain were gram-negative, rusty-coloured, strictly aerobic, chemoheterotrophic, non-budding and non-motile rods or vibrioids that possessed a thin prostheca. based on 16s rrna gene sequence comparisons, the novel strain was most closely related to the genera hyphomonas (89.4-90.9%), maricaulis (90.1-90.4%), hirschia (89.0%) and oceanicaulis (87.9%) of the family hyphomonadaceae. phylogenetic an ... | 2007 | 17978224 |
| the hierarchical system of the 'alphaproteobacteria': description of hyphomonadaceae fam. nov., xanthobacteraceae fam. nov. and erythrobacteraceae fam. nov. | phylogenetic analysis of the class 'alphaproteobacteria', including physiologically diverse species, was conducted by using small-subunit rrna gene sequences. the 16s rrna gene sequences of 261 species in the class 'alphaproteobacteria' were obtained from genbank/embl/ddbj for constructing a phylogenetic tree by using maximum-likelihood analysis. in the resulting tree, members of the class 'alphaproteobacteria' were subdivided into five major clusters, which were compared with the taxonomic outl ... | 2005 | 16166687 |
| woodsholea maritima gen. nov., sp. nov., a marine bacterium with a low diversity of polar lipids. | two cauliform bacteria (cm243t and cm251) isolated by j. poindexter from the atlantic ocean were characterized by 16s rrna gene sequencing, taqi restriction fragment length polymorphism and single-strand conformation polymorphism analyses of the internally transcribed 16s-23s rdna spacer (its1) region, analysis of fatty acids from cellular lipids, mass spectrometry of polar lipids and physiological properties. the two strains showed very low diversity of polar lipids with diacyl-sulfoquinovosyl ... | 2004 | 15280296 |
| oceanicaulis alexandrii gen. nov., sp. nov., a novel stalked bacterium isolated from a culture of the dinoflagellate alexandrium tamarense (lebour) balech. | five bacterial strains were isolated from a non-toxigenic strain of the marine dinoflagellate alexandrium tamarense (lebour) balech ccmp 116 (nepcc c116), during a survey of the diversity of bacteria associated with paralytic shellfish toxin-producing cultures of alexandrium spp. (dinophyta). all strains were strictly aerobic, gram-negative, straight or curved rods. cells were dimorphic, with stalks (or prosthecae) and non-motile or non-stalked and motile, by means of a single polar flagellum. t ... | 2003 | 14657121 |
| phylogeny of maricaulis abraham et al. 1999 and proposal of maricaulis virginensis sp. nov., m. parjimensis sp. nov., m. washingtonensis sp. nov. and m. salignorans sp. nov. | thirteen marine bacteria isolated from different locations, belonging to the genus maricaulis, were characterized by 16s rrna gene sequencing, dna-dna hybridizations and analysis of the internally transcribed 16s-23s rdna spacer (its1) region, analysis of fatty acids from total lipids, mass spectrometry of polar lipids and determination of temperature and nacl tolerances. the data obtained led to the identification of five new sulfoquinovosyl diacylglycerols, using tandem mass spectrometry, and ... | 2002 | 12508888 |
| determination of the systematic position of the genus asticcacaulis poindexter by a polyphasic analysis. | the genus asticcacaulis, to date, comprises two species of unicellular, stalked bacteria, developing a stalk at a site which is not coincidental with the centre of the pole of the cell. multiplication is similar to that demonstrated by the prosthecate species of the genera caulobacter, brevundimonas and maricaulis. a polyphasic approach, comprising 16s rrna gene sequencing, lipid analysis and nacl tolerance characterizations, was used to clarify the taxonomy of the two asticcacaulis species. fro ... | 2001 | 11211267 |
| phylogeny and polyphasic taxonomy of caulobacter species. proposal of maricaulis gen. nov. with maricaulis maris (poindexter) comb. nov. as the type species, and emended description of the genera brevundimonas and caulobacter. | the genus caulobacter is composed of prosthecate bacteria often specialized for oligotrophic environments. the taxonomy of caulobacter has relied primarily upon morphological criteria: a strain that visually appeared to be a member of the caulobacter has generally been called one without challenge. a polyphasic approach, comprising 16s rdna sequencing, profiling restriction fragments of 16s-23s rdna interspacer regions, lipid analysis, immunological profiling and salt tolerance characterizations ... | 1999 | 10425763 |