| somatic embryogenesis of abies cephalonica loud. | greek fir (abies cephalonica loudon) belongs to the mediterranean fir species and is widely distributed in the mountains of central and southern greece. considering a climatic scenario, infestation by pathogens or insects and fire episodes, it has been proposed that mediterranean firs could be in danger in some parts of their present range but, on the other hand, could also replace other species in more northern zones with temperate humid climates (e.g., silver fir, abies alba mill.). as fir spe ... | 2016 | 26619877 |
| arabinogalactan-protein epitope gal4 is differentially regulated and localized in cell lines of hybrid fir (abies alba x abies cephalonica) with different embryogenic and regeneration potential. | arabinogalactan proteins (agps) are important proteoglycans regulating somatic embryogenesis in diverse plant species. embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ecmsn) at their early developmental stages. here we show that highly embryogenic cell line ac78 of hybrid fir (abies alba x abies cephalonica) differs from very low-embryogenic cell line ac77 in the abundance, subcellular localization and deposit ... | 2008 | 17943290 |
| embryogenic culture initiation and somatic embryo development in hybrid firs (abies alba x abies cephalonica, and abies alba x abies numidica). | embryogenic cultures were established from silver fir (abies alba mill.) female megagametliophytes with developing embryos and from excised mature embryos after pollination with abies cephalonica lond. or abies numidica delann pollea the frequency of embryogenic callus formation was dependent on genotype, collection time, medium and explants used. the embryogenic callus initiation potential of megagamethophytes with developing embryos in both hybrids was higher in early july and dropped as the z ... | 1996 | 24178466 |
| bisphenol a disrupts microtubules and induces multipolar spindles in dividing root tip cells of the gymnosperm abies cephalonica. | the effects of bisphenol a (bpa), an endocrine chemical disruptor extensively used in the plastic and epoxy resin industry, on dividing root tip cells of the gymnosperm abies cephalonica loudon were investigated by confocal laser scanning microscopy after tubulin and endoplasmic reticulum immunolocalization and dna staining. microtubule arrays of all mitotic stages were disrupted within a few hours of treatment: preprophase bands exhibited asymmetric width; prometaphase, metaphase and anaphase s ... | 2016 | 26855225 |
| long-term cryopreservation of greek fir embryogenic cell lines: recovery, maturation and genetic fidelity. | in coniferous species, including greek fir (abies cephalonica loud), the involvement of somatic embryo plants in breeding and reforestation programs is dependent on the success of long-term cryostorage of embryogenic cultures during clonal field testing. in the present study on greek fir, we assayed the recovery, morphological characteristics and genetic fidelity of embryogenic cell lines 6 and 8 during proliferation and maturation after long-term cryostorage. our results indicate successful rec ... | 2011 | 21521636 |
| fulvic acid affects proliferation and maturation phases in abies cephalonica embryogenic cells. | embryogenic cell masses (ecm) of abies cephalonica were grown on proliferation media in the presence and absence of fulvic acid (fa), whose molecular composition and conformational rigidity were evaluated by cpmas-¹³c nmr spectroscopy. to assess the physiological effects of this humic material during proliferation and maturation stages of somatic embryogenesis (se), proliferation rate, proportion of consecutive developmental stages of pro-embryogenic masses (pem), cellular atp and glucose-6-phos ... | 2011 | 21458883 |
| isolation of mitochondria from embryogenic cultures of picea abies (l.) karst. and abies cephalonica loud.: characterization of a k+(atp) channel. | a valuable method to isolate and purify mitochondria from embryonal masses of two coniferous species (picea abies [l.] karst. and abies cephalonica loud.) is described. crude mitochondria from both species were shown to be intact, oxygen consuming (with malate plus glutammate, succinate and nadh as substrates) and well coupled (respiratory control ratio ca. 4). the oxidation of the substrates was only partially kcn-insensitive (alternative oxidase) in some cases. however, these fractions were co ... | 2008 | 17823801 |
| air pollution and the decline of the fir forest in parnis national park, near athens, greece. | the greek fir abies cephalonica loud. dominates the higher elevations of mount parnis, located 30 km north of athens. the fir forest is showing signs of stress and dieback commonly attributed to past mismanagement, periodic drought, and to disease. tree cores, lichens, and needle samples were collected from a southeast site facing athens and from a north-facing site. fir trees from the southeast site exhibited growth reduction since the early sixties. trees from the north site did not show growt ... | 1988 | 15092533 |