Publications

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comparative study of infection with tetrahymena of different ornamental fish species.tetrahymena is a ciliated protozoan that can infect a wide range of fish species, although it is most commonly reported in guppies (poecilia reticulata). the aim of this study was to compare the susceptibility to infection with tetrahymena of five different ornamental fish species from two different super orders. the species examined were platy (xiphophorus), molly (poecilia sphenops) and angelfish (pterophyllum scalare) of the acanthopterygii super order (which also includes guppies) and goldfi ...201424650892
survey of parasitic fauna of different ornamental freshwater fish species in iran.parasitic diseases are harmful and limiting factors in breeding and rearing ornamental fish industry. in this study, 400 apparently healthy ornamental fishes from five species (each species 80 specimens) including: goldfish (carassius auratus), guppy (poecilia reticulate), angelfish (pterophyllum scalare), discus (symphsodon discus) and sailfin mollies (poecilia latipinna) was obtained from a local ornamental fish farm in the north of iran during 2011 to 2012. the primary purpose of this study w ...201525992255
inter-specific differences in numerical abilities among teleost fish.adults, infants and non-human primates are thought to possess similar non-verbal numerical systems, but there is considerable debate regarding whether all vertebrates share the same numerical abilities. despite an abundance of studies, cross-species comparison remains difficult because the methodology employed and the context of species examination vary considerably across studies. to fill this gap, we used the same procedure, stimuli, and numerical contrasts to compare quantity abilities of fiv ...201223162517
the redox-active drug metronidazole and thiol-depleting garlic compounds act synergistically in the protist parasite spironucleus vortens.spironucleus vortens is a protozoan parasite associated with significant mortalities in the freshwater angelfish, pterophyllum scalare. control of this parasite is especially problematic due to restrictions on the use of the drug of choice, metronidazole (mtz), on fish farms. use of garlic (allium sativum) is undergoing a renaissance following experimental validations of its antimicrobial efficiency. ajoene ((e,z)-4,5,9-trithiadodeca-1,6,11-triene 9-oxide), is a stable transformation product of ...201626968264
the effects of feeding with synbiotic (pediococcus acidilactici and fructooligosaccharide) enriched adult artemia on skin mucus immune responses, stress resistance, intestinal microbiota and performance of angelfish (pterophyllum scalare).the aim of this study was to evaluate the effects of feeding on synbiotic (pediococcus acidilactici and fructooligosaccharide) enriched adult artemia franciscana on skin mucus immune responses, stress resistance, intestinal microbiota and growth performance of angelfish (pterophyllum scalare). three hundred and sixty fish with initial weight 3.2 ± 0.13 g were randomly divided into twelve aquaria (50 l) assigned to four groups in triplicates. fish were fed for 7 weeks with dietary treatments, inc ...201627150050
detection of infectious spleen and kidney necrosis virus (isknv) and turbot reddish body iridovirus (trbiv) from archival ornamental fish samples.although infections caused by megalocytiviruses have been reported from a wide range of finfish species for several decades, molecular characterisation of the viruses involved has been undertaken only on more recent cases. sequence analysis of the major capsid protein and adenosine triphosphatase genes is reported here from formalin-fixed, paraffin-embedded material from 2 archival ornamental fish cases from 1986 and 1988 in conjunction with data for a range of genes from fresh frozen tissues fr ...201628000602
pathogenesis of mixed infection by spironucleus sp. and citrobacter freundii in freshwater angelfish pterophyllum scalare.the present study was carried out to identify and describe the pathology of the freshwater angelfish pterophyllum scalare during chronic mortality in an in-door aquaculture system. scraping of the integument and gills and the collection of intestinal contents to search for external and internal parasites were performed. kidneys were collected aseptically for the microbiological analysis and the isolates were subjected to antibiotics to test for susceptibility. subsequently, necropsy for macrosco ...201627599811
growth and reproductive performance by different feed types in fresh water angelfish (pterophyllum scalare schultze, 1823).it is well established that reproduction is sensitive to the state of energy reserves, and that there is a balance between energy homeostasis and fertility. in this view, this study examined the effects of different diets on growth and reproductive performance of fresh water angelfish. twenty four pairs of angelfish (weighing 3.58 ± 0.24 g) were fed with four types of diets including live earth worm (lew), dried tubifex (dt), dried gammarus (dg) and prepared granulated feed (pgf), twice a day fo ...025610565
changes of bacterioplankton apparent species richness in two ornamental fish aquaria.we analysed the 16s rrna gene diversity within the bacterioplankton community in the water column of the ornamental fish pterophyllum scalare and archocentrus nigrofasciatus aquaria during a 60-day growth experiment in order to detect any dominant bacterial species and their possible association with the rearing organisms. the basic physical and chemical parameters remained stable but the bacterial community at 0, 30 and 60 days showed marked differences in bacterial cell abundance and diversity ...201323519098
spontaneous discrimination of small quantities: shoaling preferences in angelfish (pterophyllum scalare).the ability to quantify, i.e. to estimate quantity, may provide evolutionary advantages in some contexts and has been demonstrated in a variety of animal species. in a prior study, we showed that angelfish (pterophyllum scalare) were able to discriminate between groups (shoals) in which a large number of conspecifics swam preferring to join the larger of the two. our results implied that angelfish can compare relative shoal sizes likely on the basis of some quantitative attributes of the shoal. ...201121409443
parasitic fauna of eight species of ornamental freshwater fish species from the middle negro river in the brazilian amazon region.twenty-seven specimens of cardinal tetra paracheirodon axelrodi, 33 rosy tetra hyphessobrycon copelandi (characidae), 28 marbled hatchetfish carnegiella strigata, 26 blackwing hatchetfish carnegiella martae (gasteropelecidae), 27 bodó ancistrus hoplogenys (loricariidae), 31 brown pencilfish nannostomus eques, 38 oneline pencilfish nannostomus unifasciatus (lebiasinidae) and 13 angelfish pterophyllum scalare (cichlidae) were collected from the middle negro river, state of amazonas, brazil, for pa ...201020624347
histochemical study on the intestine goblet cells in cichlid and poecilid species (teleostei).histochemical properties of intestine goblet cells in firemouth cichlid, zebra mbuna, freshwater angelfish and platyfish are described. goblet cells occurred regularly in the epithelial cell layer throughout the entire intestine, they were strongly coloured by alcian blue at ph 2.5. this colour got gradually weaker when the ph was reduced, but still after alcian blue at ph 0.2 these cells displayed a distinct blue colour. when the goblet cells were treated with periodic acid-schiff (pas), they d ...201019833368
detection of mycobacteria in aquarium fish in slovenia by culture and molecular methods.thirty-five aquarium fish were investigated for the presence of mycobacteria by culture and molecular methods. the following species were examined: goldfish carassius auratus auratus, guppy poecilia reticulata, 4 three-spot gourami trichogaster trichopterus, dwarf gourami colisa lalia, siamese fighting fish betta splendens, freshwater angelfish pterophyllum scalare, african cichlid fish cichlidae spp., cichlid fish microgeophagus altispinosus, cichlid fish pseudotropheus lombardoi, blue streak h ...200515900685
[case report. treatment of the gillworm disease (dactylogyrosis) of some south american cichlids and catfish].the infection with gillworms (dactylogyrus sp.) causes severe problems in the keeping and breeding of tropical ornamental fishes, e.g. discus (symphisodon aequifasciata). treatment of gillworm disease with different drugs has been shown in the past to be insufficient. therefore, the effectiveness of a long-term use of praziquantel was evaluated in ornamental fish. cumulative doses up to 10 mg praziquantel/l water were tolerated without side-effects by angel fish (pterophyllum scalare), discus, a ...200312596668
a new fish cell line derived from the caudal fin of freshwater angelfish pterophyllum scalare: development and characterization.in this study, a new cell line derived from the caudal fin of the freshwater angelfish pterophyllum scalare was developed and characterized. the cell line was designated angelfish fin (aff) and subcultured 44 times since its development. these cells grew well in leibovitz's -15 medium supplemented with 10% foetal bovine saline (fbs) at 28° c and the modal chromosome number (2n) was 48. the aff cell-line is mainly comprised of epithelial cells as confirmed by immunocytological technique using ant ...201627458084
in vitro studies on optimal requirements for the growth of spironucleus vortens, an intestinal parasite of the freshwater angelfish.spironucleus vortens were cultivated in either an artificial medium at different temperatures, or in medium at various ph conditions or supplemented with different bile concentrations at 25 degrees c. temperature, ph and bile requirements for the optimal growth of the parasite were determined. parasites multiplied quickly at 28 and 31 degrees c and reached maximum numbers on day 4 of cultivation, whereafter they did not survive. at 25 degrees c, parasites survived longer than those at 28 and 31 ...200010715818
posthatching development of spinal motoneurons in the angelfish pterophyllum scalare.we investigated the posthatching developmental sequence of spinal motoneurons innervating the axial muscles in the teleost angelfish, pterophyllum scalare, by means of retrograde labeling with horseradish peroxidase. two discrete types of spinal motoneurons, primary-type motoneurons and secondary motoneurons were labeled in a temporally different sequence during the course of larval development. these two types of motoneurons were morphologically distinguishable from one other. primary-type moto ...199910343084
betanodavirus as a novel transneuronal tracer for fish.in order to obtain a potential new tool to analyze networks of the central nervous system of teleost fishes, we tested a fish-pathogenic betanodavirus, sevenband grouper nervous necrosis virus (sgnnv), as a transneuronal tracer using the freshwater angelfish pterophyllum scalare as a test animal. intravitreous injections of sgnnv into the right eye resulted first in the labelings of neuronal cell bodies in the ganglion cell layers of the retina and then those in the inner and outer nuclear layer ...200212359322
parasitic infections in freshwater ornamental fish in sri lanka.a total of 1520 ornamental fish of 13 species from 26 export farms in sri lanka were collected between october 1999 and march 2000 and examined for parasites. fish species examined were guppy poecilia reticulata, goldfish carassius auratus, platy xiphophorus maculatus, molly poecilia sphenops, angel pterophyllum scalare, swordtail xiphophorus helleri, tetras hyphessobrycon species, barbs capeota and puntius spp., gourami colisa sp., carp cyprinus carpio, fighters betta spelendens and others (bra ...200312747641
time-place learning in individually reared angelfish, but not in pearl cichlid.time-place learning based on food association was investigated in the cichlids angelfish (pterophyllum scalare) and pearl cichlid (geophagus brasiliensis) reared in isolation, therefore eliminating social influence on foraging. during a 30-day period, food was placed in one side of the aquarium (containing three compartments) in the morning and in the opposite side in the afternoon. learning was inferred by the number of correct side choices of all fish in each day of test (15th and 30th). durin ...200616952427
parasitic infections in ornamental cichlid fish in the peruvian amazon.the aim of this study was to evaluate the prevalence and seasonal distribution of the main parasite species in amazonian ornamental cichlids that affect their trade. the study was conducted from august 2007 to september 2009. we sampled 3042 specimens from 9 different species, of which 9.47% had at least one type of external parasite. 81.25% of the cases occurred in the dry season. crenicichla anthurus (28.57%) was the most parasitized, followed by aequidens diadema (26.32%), pterophyllum scalar ...201525909258
chromosomal distribution of microsatellite repeats in amazon cichlids genome (pisces, cichlidae).fish of the family cichlidae are recognized as an excellent model for evolutionary studies because of their morphological and behavioral adaptations to a wide diversity of explored ecological niches. in addition, the family has a dynamic genome with variable structure, composition and karyotype organization. microsatellites represent the most dynamic genomic component and a better understanding of their organization may help clarify the role of repetitive dna elements in the mechanisms of chromo ...201526753076
fecundity, growth, and survival of the angelfish pterophyllum scalare (perciformes: cichlidae) under laboratory conditions.the freshwater angelfishes (pterophyllum) are south american cichlids that have become very popular among aquarists, yet scarce information on their culture and aquarium husbandry exists. we studied pterophyllum scalare to analyze dietary effects on fecundity, growth, and survival of eggs and larvae during 135 days. three diets were used: a) decapsulated cysts of artemia, b) commercial dry fish food, and c) a mix diet of the rotifer brachionus plicatilis and the cladoceran daphnia magna. the ini ...200919928467
sudden weaning of angel fish pterophyllum scalare (lichtenstein) (pisces; cichlidae) larvae from brine shrimp (artemia sp) nauplii to formulated larval feed.this study investigated the effects of sudden weaning of angel fish larvae (pteraphylum scalari) from artemia nauplii to commercial larval feed. four days post hatch (dph) larvae were reared in four different weaning protocols (tr1-tr4) with triplicates in a complete randomize design. larvae in tr1 and tr4 were exclusively fed artemia nauplii and dry feed respectively. in tr2 and tr3, larvae were initially fed artemia nauplii and suddenly wean to formulated feed on 14 dph and 7 dph respectively. ...201323626927
environmental and physiological conditions affecting tetrahymena sp. infection in guppies, poecilia reticulata peters.parasitic infections caused by tetrahymena sp. constitute a serious problem in guppies, poecilia reticulata. tetrahymena was isolated from skin lesions of naturally infected guppies in a commercial aquaculture farm, cultured in vitro and used in subsequent experimental infections. in addition to guppies, angelfish, pterophyllum scalare, platyfish, xiphophorus maculates, and neontetra, paracheirodon innesi, were susceptible, whereas tilapia (oreochromis niloticus xo. aureus) was resistant. the ci ...200516266327
mycobacterium angelicum sp. nov., a non-chromogenic, slow-growing species isolated from fish and related to mycobacterium szulgai.the name 'mycobacterium angelicum' dates back to 2003 when it was suggested for a slowly growing mycobacterium isolated from freshwater angelfish. this name is revived here and the novel species is proposed on the basis of the polyphasic characterization of four strains including the original one. the four strains presented 100 % 16s rrna gene sequence similarity with mycobacterium szulgai but clearly differed from m. szulgai for the milky white aspect of the colonies. the sequence similarity wi ...201526420689
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