| differentiation of newly described antarctic bacterial isolates related to roseobacter species based on 16s-23s rdna internal transcribed spacer sequences. | the 16s-23s rdna internal transcribed spacer (its) of roseobacter denitrificans, roseobacter litoralis, ruegeria algicola and strains of the recently described species antarctobacter heliothermus and roseovarius tolerans were analysed in order to examine dna sequence variations and to draw conclusions about inter- and intraspecific relationships. a. heliothermus included four strains with an its fragment length of 1092 bp. roseovarius tolerans was described on the basis of eight strains. five of ... | 2000 | 10758903 |
| genome organization and localization of the puflm genes of the photosynthesis reaction center in phylogenetically diverse marine alphaproteobacteria. | genome organization, plasmid content and localization of the puflm genes of the photosynthesis reaction center were studied by pulsed-field gel electrophoresis (pfge) in marine phototrophic alphaproteobacteria. both anaerobic phototrophs (rhodobacter veldkampii and rhodobacter sphaeroides) and strictly aerobic anoxygenic phototrophs from the roseobacter-sulfitobacter-silicibacter clade (roseivivax halodurans, roseobacter litoralis, staleya guttiformis, roseovarius tolerans, and five new strains ... | 2004 | 15184132 |
| discovery of complex mixtures of novel long-chain quorum sensing signals in free-living and host-associated marine alphaproteobacteria. | more than 100 bacterial isolates from various marine habitats were screened for ahl production by using gfp reporter constructs based on the lasr system of pseudomonas aeruginosa and the luxr system of vibrio fischeri. of the 67 alphaproteobacteria tested, most of which belonged into the so-called roseobacter clade, 39 induced fluorescence in either one or both sensor strains up to 103-fold compared to controls. acylated homoserine lactones were identified by gc-ms analysis and shown to have cha ... | 2005 | 16283687 |
| survival of eastern oysters crassostrea virginica from three lines following experimental challenge with bacterial pathogens. | shellfish production is often affected by bacterial pathogens that cause high losses in hatcheries and nurseries. we evaluated the relative survival of larvae and juveniles of 3 crassostrea virginica oyster lines: (1) ghp, a rhode island line; (2) nehy, a line resistant to dermo and multinucleated sphere x diseases; and (3) flowers, a line resistant to roseovarius oyster disease, experimental challenge with vibrio spp. isolates re22 and re101, causative agents of bacillary necrosis in pacific oy ... | 2008 | 18500026 |
| 2,3-dihydroxypropane-1-sulfonate degraded by cupriavidus pinatubonensis jmp134: purification of dihydroxypropanesulfonate 3-dehydrogenase. | 2,3-dihydroxypropane-1-sulfonate (dhps) is a widespread intermediate in plant and algal transformations of sulfoquinovose (sq) from the plant sulfolipid sulfoquinovosyl diacylglycerol. further, dhps is recovered quantitatively during bacterial degradation of sq by klebsiella sp. strain abr11. dhps is also a putative precursor of sulfolactate in e.g. ruegeria pomeroyi dss-3. a bioinformatic approach indicated that some 28 organisms with sequenced genomes might degrade dhps inducibly via sulfolact ... | 2010 | 20150239 |
| production of free and organic iodine by roseovarius spp. | two strains of iodine-producing bacteria were isolated from marine samples. 16s rrna gene sequences indicated the strains were most closely related to roseovarius tolerans, and phylogenetic analysis indicated both belong to the same genus. 5 mm iodide inhibited the growth of strain 2s5-2 almost completely, and of strain s6v slightly. both strains produced free iodine and organic iodine from iodide. ch2i2, chi3 and ch2cli were the main organic iodines produced by strain 2s5-2, and chi3 and ch2i2 ... | 2003 | 14680698 |
| pacificibacter maritimus gen. nov., sp. nov., isolated from shallow marine sediment. | an aerobic, gram-stain-negative, non-pigmented, non-motile bacterium, strain kmm 9031(t), was isolated from a sandy sediment sample collected from the shore of the sea of japan and subjected to phenotypic and phylogenetic analysis. based on comparative 16s rrna gene sequence analysis, strain kmm 9031(t) constituted a separate phylogenetic line within the roseobacter clade of the class alphaproteobacteria, sharing highest sequence similarities with members of the genera roseovarius (92.7-95.3 %), ... | 2010 | 20601488 |
| roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline ekho lake. | eight gram-negative, aerobic, pointed and budding bacteria were isolated from various depths of the hypersaline, heliothermal and meromictic ekho lake (vestfold hills, east antarctica). the cells contained storage granules and daughter cells could be motile. bacteriochlorophyll a was sometimes produced, but production was repressed by constant dim light. the strains tolerated a wide range of temperature, ph, concentrations of artificial seawater and nacl, but had an absolute requirement for sodi ... | 1999 | 10028255 |
| microbial community structure of a heavy fuel oil-degrading marine consortium: linking microbial dynamics with polycyclic aromatic hydrocarbon utilization. | a marine microbial consortium obtained from a beach contaminated by the prestige oil spill proved highly efficient in removing the different hydrocarbon families present in this heavy fuel oil. seawater cultures showed a complete removal of all the linear and branched alkanes, an extensive attack on three to five-ring polycyclic aromatic hydrocarbons [pahs; including anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, and benzo(a)pyrene] (30-100%), and a considerable depletion of the ... | 2010 | 20528986 |
| the dddp gene of roseovarius nubinhibens encodes a novel lyase that cleaves dimethylsulfoniopropionate into acrylate plus dimethyl sulfide. | the cloned dddp gene of the marine bacterium roseovarius nubinhibens allows escherichia coli to form the volatile dimethyl sulfide (dms) from dimethylsulfoniopropionate (dmsp), an abundant anti-stress compatible solute made by many marine plankton and macroalgae. using purified dddp, we show here that this enzyme is a dmsp lyase that cleaves dmsp to dms plus acrylate. dddp forms a functional homodimeric enzyme, has a ph optimum of 6.0 and was a k(m) of approximately 14 mm for the dmsp substrate. ... | 2010 | 20378650 |
| roseovarius marinus sp. nov., isolated from seawater. | a gram-stain-negative, non-motile, ovoid- to rod-shaped bacterium, designated hdw-9(t), belonging to the class alphaproteobacteria, was isolated from seawater of the yellow sea, korea. strain hdw-9(t) grew optimally at ph 7.0-8.0, at 30 °c and with 2-3 % (w/v) nacl. neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16s rrna gene sequences showed that strain hdw-9(t) clustered with roseovarius crassostreae cv919-312(t), with which it exhibited 95.5 % 16s rrna ... | 2011 | 20348324 |
| diversity of sulfur-oxidizing bacteria in greenwater system of coastal aquaculture. | reduced sulfur compounds produced by the metabolism are the one of the major problems in aquaculture. in the present study, herbivorous fishes have been cultured as biomanipulators for secretions of slime, which enhanced the production of greenwater containing beneficial bacteria. the genes encoding soxb which is largely unique to sulfur-oxidizing bacteria (sob) due to its hydrolytic function has been targeted for examining the diversity of sob in the green water system of coastal aquaculture. n ... | 2010 | 20069462 |
| searching for a "hidden" prophage in a marine bacterium. | prophages are common in many bacterial genomes. distinguishing putatively viable prophages from nonviable sequences can be a challenge, since some prophages are remnants of once-functional prophages that have been rendered inactive by mutational changes. in some cases, a putative prophage may be missed due to the lack of recognizable prophage loci. the genome of a marine roseobacter, roseovarius nubinhibens ism (hereinafter referred to as ism), was recently sequenced and was reported to contain ... | 2010 | 19948862 |
| roseovarius nanhaiticus sp. nov., a member of the roseobacter clade isolated from marine sediment. | an aerobic, gram-staining-negative, rod or ovoid-shaped bacterial isolate, strain nh52j(t), was isolated from a sandy sediment sample from the south china sea. strain nh52j(t) exhibited tumbling motility, formed beige or faint pink colonies, gave a positive reaction in tests for catalase and oxidase and required nacl for growth. optimal growth was observed at ph 7.8-9.3, at 30 degrees c and in the presence of 2.0-4.0 % (w/v) nacl. the novel strain did not synthesize bacteriochlorophyll a, and th ... | 2010 | 19667376 |
| roseovarius halotolerans sp. nov., isolated from deep seawater. | a gram-reaction-negative, non-motile, aerobic bacterium, designated hj50(t), was isolated from deep seawater of the east sea, south korea. cells were ovoid to rod-shaped (0.5-0.8x1.3-3.0 mum), often with unequal ends, suggesting a budding mode of reproduction. the strain had an absolute requirement for sea salts and tolerated up to 20 % (w/v) sea salts. propionate, dl-lactate, 2-ketogluconate, 3-hydroxybutyrate and rhamnose were used as growth substrates, but not mannitol, salicin, 4-hydroxybenz ... | 2009 | 19625435 |
| bifurcated degradative pathway of 3-sulfolactate in roseovarius nubinhibens ism via sulfoacetaldehyde acetyltransferase and (s)-cysteate sulfolyase. | data from the genome sequence of the aerobic, marine bacterium roseovarius nubinhibens ism were interpreted such that 3-sulfolactate would be degraded as a sole source of carbon and energy for growth via a novel bifurcated pathway including two known desulfonative enzymes, sulfoacetaldehyde acetyltransferase (ec 2.3.3.15) (xsc) and cysteate sulfo-lyase (ec 4.4.1.25) (cuya). strain ism utilized sulfolactate quantitatively with stoichiometric excretion of the sulfonate sulfur as sulfate. a combina ... | 2009 | 19581363 |
| roseovarius pacificus sp. nov., isolated from deep-sea sediment. | an aerobic, gram-negative, ovoid to rod-shaped bacterial isolate, strain 81-2(t), was isolated from deep-sea sediment of the western pacific ocean. strain 81-2(t) was motile, formed faint pink colonies, and was catalase-positive, weakly positive for oxidase and required nacl for growth. it did not synthesize bacteriochlorophyll a and its dna g+c content was 62.3 mol%. the 16s rrna gene sequence of strain 81-2(t) indicated that it was a member of the roseobacter clade of the class alphaproteobact ... | 2009 | 19406803 |
| the dddp gene, encoding a novel enzyme that converts dimethylsulfoniopropionate into dimethyl sulfide, is widespread in ocean metagenomes and marine bacteria and also occurs in some ascomycete fungi. | the marine alphaproteobacterium roseovarius nubinhibens ism can produce the gas dimethyl sulfide (dms) from dimethylsulfoniopropionate (dmsp), a widespread secondary metabolite that occurs in many phytoplankton. roseovarius possesses a novel gene, termed dddp, which when cloned, confers on escherichia coli the ability to produce dms. the dddp polypeptide is in the large family of m24 metallopeptidases and is wholly different from two other enzymes, dddd and dddl, which were previously shown to g ... | 2009 | 19220400 |
| roseovarius aestuarii sp. nov., isolated from a tidal flat of the yellow sea in korea. | a gram-negative, motile, ovoid to rod-shaped bacterial strain, designated strain smk-122t, was isolated from a yellow sea tidal flat located on the coast of korea. strain smk-122t grew optimally at ph 7.0-8.0 and 30 degrees c. it contained q-10 as the predominant ubiquinone and possessed c18 : 1omega7c and c16 : 0 as the major fatty acids. the dna g+c content was 58.6 mol%. a phylogenetic analysis based on 16s rrna gene sequences showed that strain smk-122t fell within the genus roseovarius, bei ... | 2008 | 18450713 |
| a pyrene-degrading consortium from deep-sea sediment of the west pacific and its key member cycloclasticus sp. p1. | a pyrene-degrading bacterial consortium was obtained from deep-sea sediments of the pacific ocean. the consortium degraded many kinds of polycyclic aromatic hydrocarbons (pahs), including naphthalene, phenanthrene, pyrene, acenaphthene, fluorene, anthracene, fluoranthene, 2-methylnaphthalene and 2,6-dimethylnaphthalene, but it did not grow with chrysene and benzo[alpha]pyrene. with methods of plate cultivation and polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge), 72 b ... | 2008 | 18430013 |
| mesaconyl-coenzyme a hydratase, a new enzyme of two central carbon metabolic pathways in bacteria. | the coenzyme a (coa)-activated c5-dicarboxylic acids mesaconyl-coa and beta-methylmalyl-coa play roles in two as yet not completely resolved central carbon metabolic pathways in bacteria. first, these compounds are intermediates in the 3-hydroxypropionate cycle for autotrophic co2 fixation in chloroflexus aurantiacus, a phototrophic green nonsulfur bacterium. second, mesaconyl-coa and beta-methylmalyl-coa are intermediates in the ethylmalonyl-coa pathway for acetate assimilation in various bacte ... | 2008 | 18065535 |
| localization of the bacterial agent of juvenile oyster disease (roseovarius crassostreae) within affected eastern oysters (crassostrea virginica). | the bacterium roseovarius crassostreae causes seasonal mortalities among commercially produced eastern oysters (crassostrea virginica) grown in the northeastern united states. phylogenetically, the species belongs to a major lineage of marine bacteria (the roseobacter clade), within which roseovarius crassostreae is the only known pathogen to be isolated in laboratory culture. the objective of the current study was to determine the location and nature of r. crassostreae interactions with oysters ... | 2008 | 17931651 |
| use of the 16s-23s rdna internal transcribed spacer of roseovarius crassostreae for epizootiological studies of juvenile oyster disease (jod). | juvenile oyster disease (jod) in crassostrea virginica is caused by the marine bacterium roseovarius crassostreae. although the 16s rrna genes of the bacterial isolates exhibit little variation, 2 genetic signatures (gsi and gsii) may be discerned by ava i digestion of the 16s-23s internal transcribed spacer (its). in this study we analyzed isolates from jod epizootics throughout the northeastern usa (including affected adults for the first time) to better understand how oyster populations encou ... | 2007 | 17760388 |
| roseovarius sp. strain 217: aerobic taurine dissimilation via acetate kinase and acetate-coa ligase. | the genome sequence of roseovarius sp. strain 217 indicated that many pathway enzymes found in other organisms for the degradation of taurine are represented, but that a novel, apparently energy-dependent pathway is involved in the conversion of acetyl phosphate to acetyl coa. thus, an abc transporter for taurine could be postulated, while inducible taurine: pyruvate aminotransferase, alanine dehydrogenase, sulfoacetaldehyde acetyltransferase and sulfite dehydrogenase could be assayed. whereas p ... | 2007 | 17425660 |
| a pcr-based diagnostic assay for the detection of roseovarius crassostreae in crassostrea virginica affected by juvenile oyster disease (jod). | we have developed a pcr-assay for the diagnosis of juvenile oyster disease (jod) based on the detection of roseovarius crassostreae directly from affected oysters. species-specific primers are used to amplify the 16s-23s rdna internal transcribed spacer (its) of r. crassostreae, and confirmation of product identity is accomplished by restriction enzyme analysis. no false positives were obtained with either closely related bacterial species or from other dnas present in oyster samples. the assay ... | 2005 | 16385822 |
| roseovarius mucosus sp. nov., a member of the roseobacter clade with trace amounts of bacteriochlorophyll a. | among a group of marine isolates that were found to be positive for genes of the bacterial photosynthetic reaction centre, a strain was selected for characterization that was phylogenetically close to the genus roseovarius. the strain, designated dfl-24t, originated from a culture of alexandrium ostenfeldii (dinoflagellate) and contained small amounts of bacteriochlorophyll a, corresponding to about 1 % of the amount found in intensely pigmented aerobic phototrophs such as roseobacter litoralis. ... | 2005 | 16280500 |
| isolation of iodide-oxidizing bacteria from iodide-rich natural gas brines and seawaters. | iodide-oxidizing bacteria (iob), which oxidize iodide (i-) to molecular iodine (i2), were isolated from iodide-rich (63 microm to 1.2 mm) natural gas brine waters collected from several locations. agar media containing iodide and starch were prepared, and brine waters were spread directly on the media. the iob, which appeared as purple colonies, were obtained from 28 of the 44 brine waters. the population sizes of iob in the brines were 10(2) to 10(5) colony-forming units (cfu) ml(-1). however, ... | 2005 | 16047096 |
| roseicyclus mahoneyensis gen. nov., sp. nov., an aerobic phototrophic bacterium isolated from a meromictic lake. | eight strains of gram-negative bacteria able to form ring-like cells were isolated from mahoney lake, a meromictic lake in south-central british columbia, canada. all strains were pink-purple and contained bacteriochlorophyll a incorporated into the light-harvesting 1 and 2 and reaction-centre pigment-protein complexes. growth did not occur anaerobically under illuminated conditions; these strains were obligately aerobic, prompting their designation as members of the aerobic phototrophic bacteri ... | 2005 | 16014488 |
| roseovarius crassostreae sp. nov., a member of the roseobacter clade and the apparent cause of juvenile oyster disease (jod) in cultured eastern oysters. | an alpha-proteobacterium has been identified which is believed to be the causative agent of juvenile oyster disease (jod). since its first isolation in 1997, the bacterium has been recovered as the numerically dominant species from jod-affected animals throughout the north-eastern united states (maine, new york and massachusetts). colonies are usually beige to pinkish-beige, although the majority of isolates recovered in 2003 from an epizootic in martha's vineyard, massachusetts, produce colonie ... | 2005 | 16014477 |
| silicibacter pomeroyi sp. nov. and roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. | three gram-negative, rod-shaped, aerobic bacteria that were capable of degrading dimethylsulfoniopropionate (dmsp) were isolated from marine waters. these isolates (dss-3(t), dss-10 and ism(t)) exhibited the ability to demethylate and cleave dmsp, as well as to degrade other sulfur compounds related to dmsp that are cycled in marine environments. intracellular poly-beta-hydroxybutyrate inclusions, surface blebs and one polar, complex flagellum that rotated exclusively in the clockwise direction ... | 2003 | 13130004 |
| aerobic anoxygenic photosynthesis in roseobacter clade bacteria from diverse marine habitats. | the marine roseobacter clade comprises several genera of marine bacteria related to the uncultured sar83 cluster, the second most abundant marine picoplankton lineage. cultivated representatives of this clade are physiologically heterogeneous, and only some have the capability for aerobic anoxygenic photosynthesis, a process of potentially great ecological importance in the world's oceans. in an attempt to correlate phylogeny with ecology, we investigated the diversity of roseobacter clade strai ... | 2003 | 12957886 |
| hasllibacter halocynthiae gen. nov., sp. nov., a nutriacholic acid-producing bacterium isolated from the marine ascidian halocynthia roretzi. | the gram-negative, aerobic, ovoid to rod-shaped bacterial strain kme 002(t) was isolated from a marine ascidian, halocynthia roretzi, off the coast of gangneung, korea. phylogenetic analyses based on the 16s rrna gene sequence showed that this strain belongs to the family rhodobacteraceae in the class alphaproteobacteria and is closely related to species dinoroseobacter shibae, roseovarius crassostreae and pseudoruegeria aquimaris with 95.0, 94.7 and 94.5% 16s rrna gene similarity, respectively. ... | 2011 | 21551335 |
| roseovarius halocynthiae sp. nov., isolated from sea squirt halocynthia roretzi. | a gram-negative, motile, and ovoid- to rod-shaped bacterial strain, designated ma1-10(t), was isolated from a sea squirt (halocynthia roretzi) collected from the south sea, korea. strain ma1-10(t) grew optimally at ph 7.0-8.0, at 30 -¦c and in the presence of 2 % (w/v) nacl. neighbour-joining phylogenetic tree based on 16s rrna gene sequences showed that strain ma1-10(t) clustered with roseovarius crassostreae cv919-312(t), with which it exhibited 97.1 % sequence similarity, by a bootstrap resam ... | 2011 | 21669917 |
| phosphonoacetate biosynthesis: in vitro detection of a novel nadp(+)-dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of the marine roseovarius nubinhibens ism. | a novel phosphonoacetaldehyde-oxidizing activity was detected in cell-extracts of the marine bacterium roseovarius nubinhibens ism grown on 2-aminoethylphosphonic acid (2-aep; ciliatine). extracts also contained 2-aep transaminase and phosphonoacetate hydrolase activities. these findings indicate the existence of a biological route from 2-aep via phosphonoacetaldehyde for the production of phosphonoacetate, which has not previously been shown to be a natural product. the three enzymes appear to ... | 2011 | 21861368 |
| roseovarius indicus sp. nov., isolated from deep sea water of indian ocean. | a taxonomic study was carried out on strain b108t, which was isolated from a pahs-degrading consortium, enriched from the deep sea water of the indian ocean. the isolate was gram-negative, rod-shaped and non-mobile. growth was observed at salinities from 1 to 15 % and at temperatures from 10 to 39 °c, and it was unable to degrade tween 80 or gelatin. 16s rrna gene sequence comparisons showed that strain b108t was most closely related to rva. halotolerans lmg 24468t (97.1 %), followed by rva. pac ... | 2010 | 20851912 |
| dddq, a novel, cupin-containing, dimethylsulfoniopropionate lyase in marine roseobacters and in uncultured marine bacteria. | ruegeria (previously silicibacter) pomeroyi dss-3, a marine roseobacter, can catabolize dimethylsulfoniopropionate (dmsp), a compatible solute that is made in large amounts by marine plankton and algae. this strain was known to demethylate dmsp via a demethylase, encoded by the dmda gene, and it can also cleave dmsp, releasing the environmentally important volatile dimethyl sulfide (dms) in the process. we found that this strain has two different genes, dddp and dddq, which encode enzymes that c ... | 2010 | 20880330 |