| amplification of 16s rrna genes from frankia strains in root nodules of ceanothus griseus, coriaria arborea, coriaria plumosa, discaria toumatou, and purshia tridentata. | to study the global diversity of plant-symbiotic nitrogen-fixing frankia strains, a rapid method was used to isolate dna from these actinomycetes in root nodules. the procedure used involved dissecting the symbiont from nodule lobes; ascorbic acid was used to maintain plant phenolic compounds in the reduced state. genes for the small-subunit rrna (16s ribosomal dna) were amplified by the pcr, and the amplicons were cycle sequenced. less than 1 mg (fresh weight) of nodule tissue and fewer than 10 ... | 1996 | 8702283 |