| citrus psorosis virus: nucleotide sequencing of the coat protein gene and detection by hybridization and rt-pcr. | citrus psorosis virus (cpv) is a multicomponent ssrna virus with a coat protein of approximately 48 kda. the viral genome is encapsidated in short and long particles that are readily separated by sucrose density-gradient centrifugation. cpv particles are spiral filaments that are referred to as spiroviruses (sv). a cdna library of purified short particles from isolate cpv-4 was prepared in a lambda vector and screened for expression of the coat protein gene (cpg) with a monoclonal antibody to th ... | 1998 | 9634098 |
| a highly sensitive heminested rt-pcr assay for the detection of citrus psorosis virus targeted to a conserved region of the genome. | psorosis is a widespread and damaging disease of citrus in many parts of the world. the causal agent is a multipartite virus with rna genome present in very low concentration in infected citrus tissue. diagnosis is made by biological indexing on indicator citrus seedlings, but it is a slow and costly procedure and therefore it is not used generally. no sensitive wide-spectrum assay for citrus psorosis virus (cpsv) has been reported based on rt-pcr. a highly sensitive heminested rt-pcr assay is d ... | 2000 | 10644083 |
| rna 2 of citrus psorosis virus is of negative polarity and has a single open reading frame in its complementary strand. | citrus psorosis virus (cpsv) causes a citrus disease occurring worldwide. isolate cpv 4 has a genome with three single-stranded rnas. the complete sequence of rna 2 (1643 nucleotides) is reported here. northern blot hybridization with strand-specific probes showed that most of the encapsidated rna 2 is of negative polarity, although a small amount of the complementary strand may also be present in particles. the rna 2 complementary strand contained a single open reading frame encoding a protein ... | 2002 | 12075099 |
| nucleotide sequence and genomic organization of an ophiovirus associated with lettuce big-vein disease. | the complete nucleotide sequence of an ophiovirus associated with lettuce big-vein disease has been elucidated. the genome consisted of four rna molecules of approximately 7.8, 1.7, 1.5 and 1.4 kb. virus particles were shown to contain nearly equimolar amounts of rna molecules of both polarities. the 5'- and 3'-terminal ends of the rna molecules are largely, but not perfectly, complementary to each other. the virus genome contains seven open reading frames. database searches with the putative vi ... | 2002 | 12388823 |
| the partial sequence of rna 1 of the ophiovirus ranunculus white mottle virus indicates its relationship to rhabdoviruses and provides candidate primers for an ophiovirus-specific rt-pcr test. | a 4018 nucleotide sequence was obtained for rna 1 of ranunculus white mottle virus (rwmv), genus ophiovirus, representing an incomplete orf of 1339 aa. amino acid sequence analysis revealed significant similarities with rna polymerases of viruses in the family rhabdoviridae and a conserved domain of 685 aa, corresponding to the rdrp domain of those in the order mononegavirales. phylogenetic analysis indicated that the genus ophiovirus is not related to the genus tenuivirus or the family bunyavir ... | 2003 | 12756613 |
| citrus psorosis virus rna 1 is of negative polarity and potentially encodes in its complementary strand a 24k protein of unknown function and 280k putative rna dependent rna polymerase. | citrus psorosis virus (cpsv), the type member of genus ophiovirus, has three genomic rnas. complete sequencing of cpsv rna 1 revealed a size of 8184 nucleotides and northern blot hybridization with chain specific probes showed that its non-coding strand is preferentially encapsidated. the complementary strand of rna 1 contains two open reading frames (orfs) separated by a 109-nt intergenic region, one located near the 5'-end potentially encoding a 24k protein of unknown function, and another of ... | 2003 | 12951265 |
| variability of the coat protein gene of citrus psorosis virus in campania, southern italy. | variability of the coat protein (cp) gene of citrus psorosis virus (cpsv) was assessed serologically, and by sequence analyses of two genomic regions located in the 3' (region c) and 5' (region v) halves of the gene. analysis of 53 psorosis field sources from campania, italy, with 23 monoclonal antibodies revealed nine serogroups and at least ten different epitopes. sequence analysis of 19 of these sources showed limited nucleotide diversity of the cp gene in the population. diversity was slight ... | 2003 | 14579175 |
| the complete nucleotide sequence of a spanish isolate of citrus psorosis virus: comparative analysis with other ophioviruses. | the complete genomic sequence (11278 nt) of citrus psorosis virus (cpsv), isolate p-121 from spain, was determined and compared with those from isolate cpv-4 and from other ophioviruses. the three rnas of p-121 had similar size and identical organization as those of cpv-4. the 24k and the rdrp proteins were potentially encoded in the viral complementary (vc) strand of rna 1, the 54k protein potentially encoded in vcrna 2 and the coat protein encoded in vcrna 3. these four proteins from p-121 and ... | 2005 | 15449144 |
| a multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees. | a multiplex polymerase chain reaction (mpcr) assay was developed to detect six rna and one dna citrus virus: citrus leaf rugose virus (clrv), citrus psorosis virus (cpsv), citrus tatter leaf virus (ctlv), citrus tristeza virus (ctv), citrus variegation virus (cvv), citrus yellow mosaic virus (cymv), and indian citrus ringspot virus (icrsv) from citrus plants. these seven viruses are classified in six different virus genera. degenerate primers were designed based on the respective virus isolate s ... | 2005 | 15951030 |
| detection of citrus psorosis virus in the northwestern citrus production area of argentina by using an improved tas-elisa. | citrus psorosis in argentina is a serious disease. citrus is produced in two regions located in the northeast (ne) and northwest (nw) area of the country. these two areas have different climates and soil types, and therefore different citrus species and varieties are cultivated. in the ne region, psorosis is epidemic, and in the nw region, the disease was described on several occasions since 1938, but it is not observed commonly in the orchards. recently, trees with symptoms of psorosis were obs ... | 2006 | 16872685 |
| genetic variation of populations of citrus psorosis virus. | citrus psorosis virus (cpsv), the type species of genus ophiovirus, has a segmented, negative-stranded rna genome. we examined the population structure and genetic variation of cpsv in three coding regions located in rnas 1, 2 and 3, analysing 22 isolates from argentina, california, florida, italy and spain. most isolates contained a predominant sequence and some minor variants. estimations of the genetic diversity and phylogenetic clustering of isolates disclosed two populations, one comprising ... | 2006 | 16963769 |
| genetic transformation of sweet orange with the coat protein gene of citrus psorosis virus and evaluation of resistance against the virus. | citrus psorosis is a serious viral disease affecting citrus trees in many countries. its causal agent is citrus psorosis virus (cpsv), the type member of genus ophiovirus. cpsv infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootstocks. certification programs have not been sufficient to control the disease and no sources of natural resistance have been found. pathogen-derived resistance (pdr) can provide an ef ... | 2008 | 17712560 |
| isolation and identification of citrus psorosis virus egyptian isolate (cpsv-eg). | citrus psorosis ophiovirus (cpsv), is considered to be of the most serious and deter mental virus pathogen's citrus species trees in egypt. cpsv-eg was isolated from infected citrus grapefruit (c. paradisi macf.) at agric. res. centre (arc). the grapefruit which used for cpsv-eg isolate was found to be free from ctv, cevd and spiroplasma citri where as gave -ve results with dtbia, tissue print hybridization and diene's stain respectively. cpsv-eg was detected on the basis of biological indexing ... | 2008 | 19226765 |
| differential resistance to citrus psorosis virus in transgenic nicotiana benthamiana plants expressing hairpin rna derived from the coat protein and 54k protein genes. | citrus psorosis virus (cpsv), genus ophiovirus, family ophioviridae, is the causal agent of a serious disease affecting citrus trees in many countries. the viral genome consists of three ssrnas of negative polarity. post-transcriptional gene silencing (ptgs), a mechanism of plant defence against viruses, can be induced by transgenic expression of virus-derived sequences encoding hairpin rnas. since the production of transgenic citrus lines and their evaluation would take years, a herbaceous mode ... | 2009 | 19820946 |
| comparison of ftir spectra between huanglongbing (citrus greening) and other citrus maladies. | fourier transform infrared (ftir) spectroscopy has the ability to quickly identify the presence of specific carbohydrates in plant materials. the presence of the disease huanglongbing (hlb) in the leaves of infected citrus plants has a distinctive spectrum that can be used to distinguish an infected plant from a healthy plant. however, many citrus diseases display similar visible symptoms and are of concern to citrus growers. in this study several citrus diseases (citrus leaf rugose virus, citru ... | 2010 | 20438136 |
| resistance to citrus psorosis virus in transgenic sweet orange plants is triggered by coat protein-rna silencing. | the lack of naturally occurring resistance to citrus psorosis virus (cpsv) has demanded exploitation of a transgenic approach for the development of cpsv-resistant sweet orange plants. transgenic sweet orange plants producing intron-hairpin rna transcripts (ihprna) corresponding to viral cp, 54k or 24k genes were generated and analyzed at the molecular and phenotypic levels. two independent cpsv challenge assays demonstrated that expression of ihprna derived from the cp gene (ihpcp) provided a h ... | 2010 | 21084056 |
| development and validation of a multiplex reverse transcription quantitative pcr (rt-qpcr) assay for the rapid detection of citrus tristeza virus, citrus psorosis virus, and citrus leaf blotch virus. | a single real-time multiplex reverse transcription quantitative polymerase chain reaction (rt-qpcr) assay for the simultaneous detection of citrus tristeza virus (ctv), citrus psorosis virus (cpsv), and citrus leaf blotch virus (clbv) was developed and validated using three different fluorescently labeled minor groove binding qpcr probes. to increase the detection reliability, coat protein (cp) genes from large number of different isolates of ctv, cpsv and clbv were sequenced and a multiple sequ ... | 2015 | 25907469 |
| citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis a and b syndromes. | citrus psorosis virus (cpsv) is the causal agent of psorosis, a serious and widespread citrus disease. two syndromes of psorosis, psa and psb, have been described. psb is the most aggressive and rampant form. previously, we obtained pineapple sweet orange plants transformed with a hairpin construct derived from the cpsv coat protein gene (ihpcp). some of these plants were resistant to cpsv 90-1-1, a psa isolate homologous to the transgene. in this study, we found that expression of the ihpcp tra ... | 2017 | 27891561 |
| bioinformatic and mutational analysis of ophiovirus movement proteins, belonging to the 30k superfamily. | ophioviridae is a family of segmented, negative-sense, single-stranded rna plant viruses. we showed that their cell-to-cell movement protein (mp) is an isolated member of the 30k mp superfamily with a unique structural organization. all 30k mps share a core domain that contains a nearly-invariant signature aspartate. we examined its role in the mp of citrus psorosis virus (cpsv) and mirafiori lettuce big-vein virus (milbvv). alanine substitution of this aspartate prevented plasmodesmata accumula ... | 2016 | 27596536 |
| citrus psorosis virus 24k protein interacts with citrus mirna precursors, affects their processing and subsequent mirna accumulation and target expression. | sweet orange (citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. here, we show that the infection of sweet orange plants with two isolates of citrus psorosis virus (cpsv) expressing different symptomatology alters the accumulation of a set of endogenous micrornas (mirnas). within these mirnas, mir156, mir167 and mir171 were the most down-regulated, with almost a three-fold re ... | 2016 | 26033697 |
| recent advances in citrus psorosis virus. | psorosis is a globally devastating disease of citrus caused by an infectious filamentous ophiovirus, citrus psorosis virus (cpsv), which causes annual losses of about 5 % and a progressive decline of trees by affecting the conductive tissues. the disease can be harboured asymptomatically in many citrus species. in the field, the most characteristic symptoms of the disease in adult trees are bark scaling in the trunk and main branches and also internal staining in the underlying wood. the virus h ... | 2014 | 25674593 |
| a new ophiovirus is associated with blueberry mosaic disease. | blueberry mosaic disease (bmd) was first described more than 60 years ago and is caused by a yet unidentified graft transmissible agent. a combination of traditional methods and next generation sequencing disclosed the presence of a new ophiovirus in symptomatic plants. the virus was detected in all bmd samples collected from several production areas of north america and was thus named blueberry mosaic associated virus. phylogenetic analysis, supported by high bootstrap values, places the virus ... | 2014 | 24874194 |
| ophioviruses cpsv and milbvv movement protein is encoded in rna 2 and interacts with the coat protein. | citrus psorosis virus (cpsv) and mirafiori lettuce big-vein virus (milbvv), members of the ophioviridae family, have segmented negative-sense single-stranded rna genomes. to date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. here we show that the 54k protein of cpsv is encoded by rna 2 and describe its subcellular distribution. upon transient expression in nicotiana benthamiana epidermal cells the 54k protein, and also its 54k ... | 2013 | 23602594 |