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production of patchouli mild mosaic virus resistant patchouli plants by genetic engineering of coat protein precursor gene.patchouli (pogostemon cablin (blanco) benth), an aromatic crop which yields an essential oil, is widely cultivated in south-east asia. patchouli mild mosaic virus (pammv) infects patchouli plants and causes decrease in leaf biomass and essential oil yield. transgenic patchouli plants with pammv coat protein precursor (cp-p) gene have been produced by agrobacterium-mediated transformation. pammv cp-p gene integration into the patchouli genome was confirmed by the pcr method and by southern blot a ...200212449533
complete nucleotide sequence and the genome organization of patchouli mild mosaic virus rna1.the nucleotide sequence of the rna1 of patchouli mild mosaic virus (patmmv) has been determined. it contains 5,957 nucleotides excluding the 3'-terminal poly(a) tail and contains a single long open reading frame (orf) of 5,613 nucleotides extending from nucleotide 235 to 5847. the predicted polyprotein encoded by the long orf is 1,870 amino acids in length with a molecular weight of 210 kd. the conserved residues of rna-dependent rna polymerase, cysteine protease, purine ntp-binding domain and a ...200111805442
complete nucleotide sequence of the genome organization of rna2 of patchouli mild mosaic virus, a new favavirus.the complete nucleotide sequence and the genome organization of the rna 2 of a patchouli mild mosaic virus (pammv) was determined. the sequence consists of 3591 nucleotides and contains a single long open reading frame sufficient to code for 118 k protein. three proteins of 52 k, 44 k and 22 k could be encoded by the pammv rna 2 genome. our analysis of the n-terminal sequences of two species of coat protein (cp) allowed precise location of the cp cistrons within the polyprotein. 44 k and 22 k pr ...19989930198
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