production of patchouli mild mosaic virus resistant patchouli plants by genetic engineering of coat protein precursor gene. | patchouli (pogostemon cablin (blanco) benth), an aromatic crop which yields an essential oil, is widely cultivated in south-east asia. patchouli mild mosaic virus (pammv) infects patchouli plants and causes decrease in leaf biomass and essential oil yield. transgenic patchouli plants with pammv coat protein precursor (cp-p) gene have been produced by agrobacterium-mediated transformation. pammv cp-p gene integration into the patchouli genome was confirmed by the pcr method and by southern blot a ... | 2002 | 12449533 |
complete nucleotide sequence and the genome organization of patchouli mild mosaic virus rna1. | the nucleotide sequence of the rna1 of patchouli mild mosaic virus (patmmv) has been determined. it contains 5,957 nucleotides excluding the 3'-terminal poly(a) tail and contains a single long open reading frame (orf) of 5,613 nucleotides extending from nucleotide 235 to 5847. the predicted polyprotein encoded by the long orf is 1,870 amino acids in length with a molecular weight of 210 kd. the conserved residues of rna-dependent rna polymerase, cysteine protease, purine ntp-binding domain and a ... | 2001 | 11805442 |
complete nucleotide sequence of the genome organization of rna2 of patchouli mild mosaic virus, a new favavirus. | the complete nucleotide sequence and the genome organization of the rna 2 of a patchouli mild mosaic virus (pammv) was determined. the sequence consists of 3591 nucleotides and contains a single long open reading frame sufficient to code for 118 k protein. three proteins of 52 k, 44 k and 22 k could be encoded by the pammv rna 2 genome. our analysis of the n-terminal sequences of two species of coat protein (cp) allowed precise location of the cp cistrons within the polyprotein. 44 k and 22 k pr ... | 1998 | 9930198 |