| range of phytoplasma concentrations in various plant hosts as determined by competitive polymerase chain reaction. | abstract for competitive polymerase chain reaction (pcr), an internal standard dna template was developed that consisted of a highly conserved, internally deleted 16s rdna fragment of an aster yellows phytoplasma. the internal standard was calibrated using a quantified culture of acholeplasma laidlawii. serial dilutions of the internal standard and fixed amounts of target templates from infected plants were coamplified with the same primers, and the products obtained were quantified using an enz ... | 2000 | 18944479 |