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tessaracoccus bendigoensis gen. nov., sp. nov., a gram-positive coccus occurring in regular packages or tetrads, isolated from activated sludge biomass.an isolate of a gram-positive bacterium, designated strain ben 106t, was obtained in pure culture by micromanipulation of a biomass sample obtained from a laboratory-scale sequencing batch reactor. this isolate grew axenically as cocci or clusters of cocci arranged in regular tetrads and was morphologically similar to the dominant organism observed in the biomass. this morphology resembled that of some gram-positive and -negative bacteria and the so-called 'g-bacteria' commonly seen in activated ...199910319465
granulicoccus phenolivorans gen. nov., sp. nov., a gram-positive, phenol-degrading coccus isolated from phenol-degrading aerobic granules.a gram-positive bacterium, designated strain pg-02(t), was isolated by serial dilution from aerobic granules obtained from a laboratory-scale sequencing batch reactor for bioremediation of phenolic wastewater. strain pg-02(t) grew axenically as cocci and is an oxidase-negative and catalase-positive, non-motile facultative anaerobe. it does not reduce nitrate and grows between 15 and 37 degrees c, with an optimum temperature of 30 degrees c. the ph range for growth is between 5.0 and 8.5, with an ...200717392196
culturable bacterial diversity at the princess elisabeth station (utsteinen, sør rondane mountains, east antarctica) harbours many new taxa.we studied the culturable heterotrophic bacterial diversity present at the site of the new princess elisabeth station at utsteinen (dronning maud land, east antarctica) before construction. about 800 isolates were picked from two terrestrial microbial mat samples after incubation on several growth media at different temperatures. they were grouped using rep-pcr fingerprinting and partial 16s rrna gene sequencing. phylogenetic analysis of the complete 16s rrna gene sequences of 93 representatives ...201121501941
diagnosis and quantification of glycerol assimilating denitrifying bacteria in an integrated fixed-film activated sludge reactor via 13c dna stable-isotope probing.glycerol, a byproduct of biodiesel and oleo-chemical manufacturing operations, represents an attractive alternate to methanol as a carbon and electron donor for enhanced denitrification. however, unlike methanol, little is known about the diversity and activity of glycerol assimilating bacteria in activated sludge. in this study, the microbial ecology of glycerol assimilating denitrifying bacteria in a sequencing batch integrated fixed film activated sludge (sb-ifas) reactor was investigated usi ...201021053955
salinarimonas ramus sp. nov. and tessaracoccus oleiagri sp. nov., isolated from a crude oil contaminated saline soil.four bacterial strains, sl014b-41a4t, sl014b-20a1t, sl014b-76a1 and sl014b-79a, isolated from a crude oil contaminated saline soil of shengli oilfield in china, were investigated using a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequences showed strain sl014b-41a4t belonged to the genus salinarimonas in the order rhizobiales, with the highest similarity of 98.3 % to type strain salinarimonas rosea yim yd3t. the dna-dna relatedness of strain sl014b-41a4t to s. rosea yim yd ...201020802058
description of tessaracoccus profundi sp.nov., a deep-subsurface actinobacterium isolated from a chesapeake impact crater drill core (940 m depth).a novel actinobacterium, designated cb31(t), was isolated from a 940 m depth sample of a drilling core obtained from the chesapeake meteor impact crater. the strain was isolated aerobically on r2a medium agar plates supplemented with nacl (20 g l(-1)) and mgcl2 x 6 h2o (3 g l(-1)). the colonies were circular, convex, smooth and orange. cells were slightly curved, rod-shaped in young cultures and often appeared in pairs. in older cultures cells were coccoid. cells stained gram-positive, were non- ...200919669589
tessaracoccus lubricantis sp. nov., isolated from a metalworking fluid.a gram-positive-staining, coccoid-shaped, oxidase-negative, non-spore-forming, non-motile bacterium, strain kss-17se(t), was isolated from a metalworking fluid. on the basis of its major fatty acid (ai-c(15 : 0)) and 16s rrna gene sequence similarity, the strain grouped with tessaracoccus bendigoensis and tessaracoccus flavescens, sharing 95.3 and 97.4 % 16s rrna gene sequence similarity with the respective type strains. similarities with other established species of the genera luteococcus, prop ...200919502351
tessaracoccus flavescens sp. nov., isolated from marine sediment.a novel actinobacterium, designated strain sst-39t, was isolated from a marine sediment sample collected in jeju, republic of korea. cells were facultatively anaerobic, gram-positive, non-endospore-forming, non-motile rods. colonies were circular, slightly convex, opaque and brilliant yellow. the cell-wall peptidoglycan of the organism contained ll-diaminopimelic acid as the diagnostic diamino acid. polar lipids included phosphatidylglycerol and diphosphatidylglycerol. the predominant menaquinon ...200818398170
brooklawnia cerclae gen. nov., sp. nov., a propionate-forming bacterium isolated from chlorosolvent-contaminated groundwater.two novel facultatively anaerobic bacterial strains, bl-34(t) and bl-35, isolated from groundwater contaminated by a mixture of chlorosolvents were characterized using a polyphasic approach. the two strains exhibited essentially identical taxonomic features except for a vitamin b(12) requirement by strain bl-35 for optimal growth. phylogenetically, the isolates were affiliated with members of the family propionibacteriaceae and were placed in a phylogenetic branch adjacent to, but distinct from, ...200616902040
three isolates of novel polyphosphate-accumulating gram-positive cocci, obtained from activated sludge, belong to a new genus, tetrasphaera gen. nov., and description of two new species, tetrasphaera japonica sp. nov. and tetrasphaera australiensis sp. nov.two isolates of gram-positive cocci (ben 109t and ben 110) which could accumulate polyphosphate and were microscopically similar in appearance to so-called 'g-bacteria', appearing as tetrads, were isolated from samples of activated sludge biomass by micromanipulation and grown in axenic culture. on the basis of their phenotypic and chemotaxonomic characters and 16s rdna sequences, these isolates, together with strain t1-x7t isolated and described previously in japan, belong to a new genus. these ...200010758865
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