| isolation, cdna cloning and gene expression of an antibacterial protein from larvae of the coconut rhinoceros beetle, oryctes rhinoceros. | an antibacterial protein, designated rhinocerosin, was purified to homogeneity from larvae of the coconut rhinoceros beetle, oryctes rhinoceros immunized with escherichia coli. based on the amino acid sequence of the n-terminal region, a degenerate primer was synthesized and reverse-transcriptase pcr was performed to clone rhinocerosin cdna. as a result, a 279-bp fragment was obtained. the complete nucleotide sequence was determined by sequencing the extended rhinocerosin cdna clone by 5' rapid ... | 1998 | 9738915 |
| genomic analysis of oryctes rhinoceros virus reveals genetic relatedness to heliothis zea virus 1. | oryctes rhinoceros virus (orv) is an unassigned invertebrate dsdna virus with enveloped and rod-shaped virions. two cloned psti fragments, c and d, of orv dna have been sequenced, consisting of 19,805 and 17,146 bp, respectively, and comprising about 30% of the orv genome. for each of the two fragments, 20 open reading frames (orfs) of 150 nucleotides or greater with no or minimal overlap were predicted. ten of the predicted 40 orfs revealed significant similarities to heliothis zea virus 1 (hzv ... | 2007 | 17106621 |
| biology and management of palm dynastid beetles: recent advances. | coconut, oil, and date palms are important crops in the tropics and are attacked by dynastids that cause loss of production or death of hosts. knowledge of their breeding sites has been extended since a previous review in 1980. the fungus metarhizium anisopliae has potential as a biopesticide against immature stages in friable breeding sites. the molecular biology and ultrastructure of oryctes rhinoceros nudivirus (ornv), disseminated by adults, have been studied, and this pathogen can reduce o. ... | 2013 | 23317044 |
| prospects of using metarhizium anisopliae to check the breeding of insect pest, oryctes rhinoceros l. in coconut leaf vermicomposting sites. | during vermicomposting of coconut leaves by the earthworm eudrilus sp., oryctes rhinoceros l. (rhinoceros beetle), an insect pest of palms, was found to breed in the decomposing organic material. metarhizium anisopliae var. major was tried as a biocontrol agent for management of this pest. the effect of pathogen at spore loads of 10(3), 10(4) and 10(5) per 10 g of substrate was tested in laboratory on eudrilus sp. kept with o. rhinoceros grubs and on eudrilus sp. alone for the pathogenic capabil ... | 2006 | 16230009 |
| scarabaecin, a novel cysteine-containing antifungal peptide from the rhinoceros beetle, oryctes rhinoceros. | a novel antifungal peptide, scarabaecin (4080da), was isolated from the coconut rhinoceros beetle, oryctes rhinoceros. scarabaecin cdna was cloned by reverse transcriptase-polymerase chain reactions (rt-pcr) using a primer based on the n-terminal amino acid sequence. the amino acid sequence deduced from scarabaecin cdna showed no significant similarity to those of reported proteins. chemically synthesized scarabaecin indicated antifungal activity against phytopathogenic fungi such as pyricularia ... | 2003 | 12859949 |
| purification, cdna cloning and modification of a defensin from the coconut rhinoceros beetle, oryctes rhinoceros. | a novel member of the insect defensins, a family of antibacterial peptides, was purified from larvae of the coconut rhinoceros beetle, oryctes rhinoceros, immunized with escherichia coli. a full-size cdna was cloned by combining reverse-transcription pcr (rt-pcr), and 5'- and 3'-rapid amplification of cdna ends (race). analysis of the o. rhinoceros defensin gene expression showed it to be expressed in the fat body and hemocyte, midgut and malpighian tubules. o. rhinoceros defensin showed strong ... | 1999 | 10561605 |
| evolution in oryctes baculovirus: rate and types of genomic change. | three cloned strains of oryctes baculovirus were released into a previously unexposed population of the host insect, the coconut palm rhinoceros beetle, oryctes rhinoceros. the experiment was conducted on meemu atoll in the maldive islands. viruses were isolated from the beetle population at 1 year, 1.75 years, and 4 years after release. no changes in genotype were observed in viruses isolated after 1 and 1.75 years. after 4 years, however, three types of genomic change had occurred. a recombina ... | 1990 | 2403706 |
| [the effect in vitro of the toxins of the mushroom metarrhizium anisopliae (metsch.) sorok. on the hemocytic reaction of oryctes rhinoceros l. (coleoptera)]. | the filtrate of metarhizium cultures contains toxic-substances inhibiting the process of formation of granuloma by oryctes in vitro. this inhibition results from marked alterations of both nucleus and cytoplasm of hemocytes under the action of the fungal toxins. | 1975 | 809180 |
| a virus disease of the indian rhinoceros beetle, oryctes rhinoceros(linnaeus), caused by a new type of insect virus, rhabdionvirus oryctes gen. n., sp. n. | | 1966 | 5905536 |
| isolation, cdna cloning, and structure-based functional characterization of oryctin, a hemolymph protein from the coconut rhinoceros beetle, oryctes rhinoceros, as a novel serine protease inhibitor. | we isolated oryctin, a 66-residue peptide, from the hemolymph of the coconut rhinoceros beetle oryctes rhinoceros and cloned its cdna. oryctin is dissimilar to any other known peptides in amino acid sequence, and its function has been unknown. to reveal that function, we determined the solution structure of recombinant (13)c,(15)n-labeled oryctin by heteronuclear nmr spectroscopy. oryctin exhibits a fold similar to that of kazal-type serine protease inhibitors but has a unique additional c-termi ... | 2010 | 20630859 |
| a dna virus of drosophila. | little is known about the viruses infecting most species. even in groups as well-studied as drosophila, only a handful of viruses have been well-characterized. a viral metagenomic approach was used to explore viral diversity in 83 wild-caught drosophila innubila, a mushroom feeding member of the quinaria group. a single fly that was injected with, and died from, drosophila c virus (dcv) was added to the sample as a control. two-thirds of reads in the infected sample had dcv as the best blast hit ... | 2011 | 22053195 |
| the genome of oryctes rhinoceros nudivirus provides novel insight into the evolution of nuclear arthropod-specific large circular double-stranded dna viruses. | the oryctes rhinoceros nudivirus (ornv) is a dsdna virus with enveloped, rod-shaped virions. its genome is 127,615-ábp in size and contains 139 predicted protein-coding open reading frames (orfs). in-depth genome sequence comparisons revealed a varying number of shared gene homologues, not only with other nudiviruses (nvs) and baculoviruses, but also with other arthropod-specific large dsdna viruses, including the so-called monodon baculovirus (mbv), the salivary gland hypertrophy viruses (sghvs ... | 2011 | 21380757 |
| nudiviruses and other large, double-stranded circular dna viruses of invertebrates: new insights on an old topic. | nudiviruses (nvs) are a highly diverse group of large, circular dsdna viruses pathogenic for invertebrates. they have rod-shaped and enveloped nucleocapsids, replicate in the nucleus of infected host cells, and possess interesting biological and molecular properties. the unassigned viral genus nudivirus has been proposed for classification of nudiviruses. currently, the nudiviruses comprise five different viruses: the palm rhinoceros beetle virus (oryctes rhinoceros nv, ornv), the hz-1 virus (he ... | 2009 | 19460388 |
| sequencing of the large dsdna genome of oryctes rhinoceros nudivirus using multiple displacement amplification of nanogram amounts of virus dna. | the genomic sequence analysis of many large dsdna viruses is hampered by the lack of enough sample materials. here, we report a whole genome amplification of the oryctes rhinoceros nudivirus (ornv) isolate ma07 starting from as few as about 10 ng of purified viral dna by application of phi29 dna polymerase- and exonuclease-resistant random hexamer-based multiple displacement amplification (mda) method. about 60 microg of high molecular weight dna with fragment sizes of up to 25 kbp was amplified ... | 2008 | 18598718 |
| microbial control of arthropod pests of tropical tree fruits. | a multitude of insects and mites attack fruit crops throughout the tropics. the traditional method for controlling most of these pests is the application of chemical pesticides. growing concern on the negative environmental effects has encouraged the development of alternatives. inundatively and inoculatively applied microbial control agents (virus, bacteria, fungi, and entomopathogenic nematodes) have been developed as alternative control methods of a wide variety of arthropods including tropic ... | 2007 | 17607448 |
| the genome of gryllus bimaculatus nudivirus indicates an ancient diversification of baculovirus-related nonoccluded nudiviruses of insects. | the gryllus bimaculatus nudivirus (gbnv) infects nymphs and adults of the cricket gryllus bimaculatus (orthoptera: gryllidae). gbnv and other nudiviruses such as heliothis zea nudivirus 1 (hznv-1) and oryctes rhinoceros nudivirus (ornv) were previously called "nonoccluded baculoviruses" as they share some similar structural, genomic, and replication aspects with members of the family baculoviridae. their relationships to each other and to baculoviruses are elucidated by the sequence of the compl ... | 2007 | 17360757 |
| the oryctes virus: its detection, identification, and implementation in biological control of the coconut palm rhinoceros beetle, oryctes rhinoceros (coleoptera: scarabaeidae). | in view of the increasing and devastating damage by rhinoceros beetle (oryctes rhinoceros) to coconut palms in the middle of last century, many efforts were made to find an efficient natural control factor against this pest, which could not be controlled by pesticides. the basic procedures of these monitoring programmes are outlined together with the final detection of a virus disease in oil palm estates in malaysia in 1963. in extensive laboratory studies, the virus was isolated and identified ... | 2005 | 16039308 |