| development of trypanosoma cruzi in the vector in the absence of blood. | first stage nymphs of triatoma infestans and dipetalogaster maximus which had never fed after egg hatching, were allowed to ingest by artificial feeding a blood-free suspension of trypanosoma cruzi trypomastigotes collected from experimentally infected mice. a high percentage of the vectors acquired a normal infection and produced infective stages. the parasite's development in the vector did not require blood but may have needed the presence of unknown factors secreted and/or excreted in the in ... | 1978 | 32750 |
| [new comparative studies between dipetalogaster maximus and triatoma infestans in the xenodiagnosis of chronic human chagas infection]. | | 1978 | 98820 |
| [preliminary study on the use of dipetalogaster maximus (uhler, 1894) (triatominae) in the xenodiagnostic technic in the chronic form of chagas' disease]. | | 1978 | 103171 |
| improvements in routine xenodiagnosis with first instar dipetalogaster maximus (uhler 1894) (triatominae). | first instar dipetalogaster maximus proved as effective as third instar triatoma infestans in detecting circulating trypanosoma cruzi in patients with chronic infections examined in a routine xenodiagnostic laboratory. since rearing costs are diminished, d. maximus has now replaced t. infestans as the xenodiagnostic agent in our laboratory. however, further work is needed to define the optimal conditions necessary when using this species in xenodiagnosis. | 1979 | 111570 |
| dipetalogaster maximus (hemiptera, triatominae) for xenodiagnosis of patients with serologically detectable trypanosoma cruzi infection. | in patients serologically positive for trypanosoma cruzi infection the three bug species/instar combinations used in xenodiagnosis showed third-instar dipetalogaster maximus to be more efficient in detecting circulating trypanosomes than the first instar of the same species which, in turn, is more sensitive than third-instar triatoma infestans. the sensitivity of the pool technique of faecal examination compared with individual dissection was investigated. four pool examinations (the product of ... | 1979 | 119338 |
| report on a field collection of dipetalogaster maximus (hemiptera triatominae) (uhler, 1894). | | 1979 | 120977 |
| [studies on the persistence of the infectivity of trypanosoma cruzi. ii. persistence of infectivity of t. cruzi in dead bugs (author's transl)]. | investigations into the viability time of t. cruzi in dead bugs experimentally infected with "peru" strain showed a maximum of 9 days for dipetalogaster maximus and 8 for triatoma infestans maintained at ambient temperature (26 degrees c). on refrigeration survival was increased to 60 days. in routine xenodiagnosis in the refrigerator 90 day survival was noted of both t. cruzi and the vector bug. | 1979 | 396604 |
| [bionomic studies of dipetalogaster maximus (uhler, 1894) (hemiptera, reduviidae, triatominae). iii. population dynamics]. | a population dynamics study of d. maximus was carried out under laboratory conditions (28 degrees c e 65% +/- 5% u.r.), and the methodology was the same that has been used for rearing these insects. in order to evaluate the population growth rate of this species, during a 24 months period, five colonies started with a couple recently emerged were observed. each couple (a male and a female) was maintained in a glass container measuring 20 cm of diameter and 20 cm in height with filter paper on th ... | 1992 | 1343801 |
| effect of azadirachtin on the development of trypanosoma cruzi in different species of triatomine insect vectors: long-term and comparative studies. | studies were carried out on the effects of azadirachtin on the course of trypanosoma cruzi infection in the gut of different triatomine vector species. in rhodinus prolixus the development of t. cruzi clone dm28c decreased in a dose-dependent manner, and the ed50 of this inhibitory effect was 0.25 microgram azadirachtin/ml bloodmeal. using this insect, we conducted a long-term experiment which showed that azadirachtin (1.0 microgram/ml bloodmeal) completely blocks the development of t. cruzi eve ... | 1992 | 1401990 |
| the life cycle of trypanosoma cruzi under axenic conditions. | the complete life cycle of trypanosoma cruzi under germfree (gf) conditions was achieved. the reduvidae insects dipetalogaster maximus, reared under axenic conditions were infected with trypanosoma cruzi, strain cl, by feeding on gf infected mice. axenic trypomastigotes from macerated gnotobiotic insects were used to infect gf and conventional (cv) mice by intraperitoneal, ocular, and oral routes. infection, followed by blood parasitemia, was obtained in almost all the cases. however, chagas' di ... | 1992 | 1479302 |
| immunization and challenge of mice with insect-derived metacyclic trypomastigotes of trypanosoma cruzi. | infection with trypanosoma cruzi was established in the reduviid vector, dipetalogaster maximus, by repeated feedings on mice with high parasitaemias, and metacyclic trypomastigotes (imt) were collected in insect urine after blood meals. the infectivity of imt in mice was assessed by placing varying numbers of organisms, ranging from 5 to 5000, on to the conjunctivae or oral mucosa of anaesthetized animals. half of the mice exposed to as few as 20 imt by either route became parasitized, and the ... | 1990 | 2107500 |
| cannibalism and coprophagy are modes of transmission of blastocrithidia triatomae (trypanosomatidae) between triatomines. | transovarial transmission was not detectable among blastocrithidia triatomae-infected triatoma infestans. rather, b. triatomae was transmitted directly between triatomines by cannibalism and coprophagy. cannibalism conditions that excluded coprophagy always resulted in an infection of dipetalogaster maxima. the efficiency of transmission was not influenced by the blood source--mice or chickens--fed to the infected donor bugs although chicken blood lyses the epimastigotes of the stomach populatio ... | 1989 | 2498511 |
| [isolation of trypanosoma cruzi samples by xenodiagnosis and hemoculture from patients with chronic chagas' disease]. | fifty nine chronic chagasic patients were simultaneously submitted to xenodiagnosis and hemoculture for trypanosoma cruzi samples isolations. the xenodiagnosis was done with 40 panstrongylus megistus, triatoma infestans and dipetalogaster maximus nymphs, performing 120 triatomines. groups of 10 insects per specie were dissecated and the intestinal content pooled and examined, after previous trituration and homogenization. the microscopically negative material was seed into lit medium and examine ... | 1989 | 2517566 |
| direct transmission of trypanosoma cruzi between vectors of chagas' disease. | trypanosoma cruzi was transmitted directly between triatomines by cannibalism or coprophagy. different conditions involving cannibalism that excluded coprophagy were studied in dipetalogaster maximus. infections occurred if an uninfected donor bug sucked infectious blood and if this blood was taken up from the stomach by a cannibalistic bug. if the donor was infected and sucked uninfected blood afterwards, the source of the uninfected blood determined the transmission rate: if the uninfected blo ... | 1988 | 2896441 |
| dipetalogaster maxima or d. maximus as a xenodiagnostic agent. | | 1986 | 3150587 |
| morphological evidence by scanning electron microscopy of excretion of metacyclic forms of trypanosoma cruzi in vector's urine. | comparison by scanning electron microscopy (sem) of trypanosoma cruzi flagellates attached to the cuticle of the rectal gland of infected dipetalogaster maxima nymphs, showed marked differences before and after feeding. before feeding numerous metacyclic trypomastigotes were observed among the abundant epimastigotes that formed the carpet of flagellates. on the other hand, in insects that were allowed to urinate for 24 hours after a meal, the metacyclics were scarce, indicating that they had bee ... | 1988 | 3152276 |
| rhodnius neivai: a new experimental vector of trypanosoma rangeli. | rhodnius neivai was as efficient as rhodnius prolixus and rhodnius robustus in transmitting by bite a colombian strain of trypanosoma rangeli following its inoculation into the hemocoel. under conditions of the study the strain of t. rangeli had a high and constant infectivity to the salivary glands of r. prolixus, its natural vector in colombia. six species of triatoma and dipetalogaster maximus likewise inoculated did not develop metatrypomastigotes in the salivary glands. of the 12 known spec ... | 1986 | 3518505 |
| epizootiology of trypanosoma cruzi in southwestern north america. x. the biosystematics of dipetalogaster maximus in mexico (hemiptera: reduviidae) (kinetoplastida: trypanosomidae). | | 1967 | 4963987 |
| [the extradigestive cycle of trypanosoma cruzi in triatomidae. i. finding of amastigote forms in the stomach walls of dipetalogaster maximus]. | | 1982 | 6763729 |
| growth and development of two trypanosoma cruzi clones in the arthropod dipetalogaster maximus. | trypomastigotes of single-cell-isolate trypanosoma cruzi clones wa-250/1 and esmereldo/2 were used to infect third-instar dipetalogaster maximus. the level of infection was directly proportional to the concentration of trypomastigotes present in the blood meal. the minimum infectious dose for d. maximus was calculated as 5 organisms/ml. clone wa-250/1 underwent epimastigote-to-metacylic trypomastigote transformation over the 30-day course of the experiments; clone esmeraldo/2 did not undergo tra ... | 1982 | 7041664 |
| [importance of the method for obtaining feces from triatominae in the assessment of triatomine susceptibility to trypanosoma cruzi]. | two patients on the acute phase of chagas' disease were submitted to xenodiagnosis examination with different species of triatomine bugs, in order to study bug susceptibility to trypanosoma cruzi. species used were dipetalogaster maximus, rhodnius neglectus, r. prolixus, r. robustus, triatoma infestans and t. rubrovaria. both the number of infected bugs, and the number of excreted trypanosomes by bug, were used as parameters for evaluation of species susceptibility. xenodiagnosis reading was per ... | 1993 | 8115684 |
| [morphological study of ovum and nymphs of dipetalogaster maximus (uhler, 1894) (hemiptera, reduviidae, triatominae)]. | intending to provide new parameters to be used in triatomines' taxonomy, vectors of chaga's disease and to amplify a large range of knowledge about this species, a study of the structure of the egg and external morphology of the five nymphal instars was done. the eggs show under optical microscope (m.o.), the chorium's surface of the body and the operculum formed by poligonal areas, clearer under scanning electronic microscope (mev). the apex of the third rostrum segment in all 5 instars nymphs, ... | 1993 | 8209030 |
| [a comparative study between natural and artificial xenodiagnosis in chronic chagas' disease patients]. | in order to study the sensitivity of the xenodiagnosis technique a comparison between natural and artificial xenodiagnosis methods was performed in 57 chronic phase chagasic patients (31 female), with ages ranging from 26 to 83 years. all patients had demonstrable antibodies against trypanosoma cruzi. forty first instar nymphs of dipetalogaster maximus were used for each of both methods and for each patient. the positivity of xenodiagnosis artificial was significantly higher than the routine tes ... | 1995 | 8668837 |
| human immune response to triatomine embryo extract. | dipetalogaster maximus embryo extracts were used to stimulate peripheral blood mononuclear cells (pbmc) and in elisa with sera either from trypanosoma cruzi infected or non-infected individuals. the results showed that there was significant proliferative response and high antibody, titers in sera of chagasic patients. | 1996 | 8993108 |
| metabolic post-feeding changes in fat body and hemolymph of dipetalogaster maximus (hemiptera: reduviidae). | lipids and glycogen in fat body as well as the modifications in the wet weight of this organ were evaluated in an unfed insect, dipetalogaster maximus, on day 5 after adult ecdysis (time 0) and during a 30-day period after ingestion of blood meal, total lipids, high density lipophorin (hdlp), carbohydrates, total proteins and uric acid were determined in the hemolymph during the same period. fat body wet weight was maximum on day 10 post-feeding and represented on day only 42% of the maximum wei ... | 1998 | 9698899 |
| effect of fasting on the composition of the fat body lipid of dipetalogaster maximus, triatoma infestans and panstrongylus megistus (hemiptera: reduviidae). | modifications in content and lipid composition induced by fasting were examined in fat bodies from adults of triatominae, dipetalogaster maximus, triatoma infestans and panstrongylus megistus. with fasting, total lipid stores dropped approximately 50% for t. infestans and more than 70% for p. megistus. total lipids analyzed by thin layer chromatography and fractionated by column chromatography on unisil showed triacylglycerols as the main component in the three species, although p. megistus show ... | 1998 | 9810719 |
| beauveria bassiana (hyphomycetes) as a possible agent for biological control of chagas disease vectors. | beauveria bassiana (balsamo) vuillemin (isolate cg306) was tested on 3rd instars of 9 triatoma spp., 4 rhodnius spp., 2 panstrongylus spp. and dipetalogaster maxima (uhler) at 25 degrees c and 50% rh. quantitative sporulation of the fungus on cadavers was studied at 25 degrees c and 97% rh. mortality, estimates of survival time, and conidial production on cadavers differed significantly among the genera and species tested. panstrongylus herreri wygodzinsky, dipetalogaster maxima, triatoma pictur ... | 1998 | 9835689 |
| dipetalogastin, a potent thrombin inhibitor from the blood-sucking insect. dipetalogaster maximus cdna cloning, expression and characterization. | a cdna coding for the thrombin inhibitor dipetalogastin has been isolated from a stomach library of dipetalogaster maximus, a blood-sucking insect. the open reading frame of the cloned inhibitor cdna codes for a protein of 344 amino-acid residues. sequence analysis reveals the existence of three repeated homologous main regions, indicating that the inhibitor consists of three domains. each domain shows a double-headed structure with an internal sequence homology like rhodniin, the thrombin inhib ... | 1999 | 10561601 |
| effects of temperature and ph on hexokinase from the flight muscles of dipetalogaster maximus (hemiptera: reduviidae). | effects of temperature and ph on the catalytic properties of hexokinase (hk, ec 2.7.1.1) from the flight muscles of dipetalogaster maximus (uhler) were studied. the enzyme showed a hyperbolic behavior with its two substrates (glucose and atp). there was no inhibition by glucose. apparent km and vmax increased as ph increased from 7.0 to 8.5. catalytic efficiency was lowest at ph 7.0. km, vmax, and catalytic efficiency were higher at 37 degrees c than at 30 and 20 degrees c. there was marked inhi ... | 2000 | 11004779 |
| presence of a fatty acid-binding protein and lipid stores in flight muscles of dipetalogaster maximus (hemiptera: reduviidae). | a fatty acid-binding protein (fabp) from the cytosolic fraction of the triatomine dipetalogaster maximus (uhler) flight muscles was purified by a procedure based on gel filtration, reverse-phase high performance liquid chromatography, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the protein has an apparent molecular mass of 14 kda, and its n-terminus is unblocked. its n-terminal sequence was obtained by submitting an sds-page band blotted onto a polyvinylidene difluo ... | 2000 | 11126554 |
| comparative study of enzymes in testes and ovaries from adult dipetalogaster maximus (uhler) and triatoma infestans (klug) (hemiptera: reduviidae). correlation with fine structural organization. | activities of hexokinase (hk), glucose-6-phosphate dehydrogenase (g6pdh), fructose-6-phosphate kinase (f6pk), glutamate dehydrogenase (glutdh), aspartate aminotransferase (aat), malate dehydrogenase (mdh) and glycerol-3-phosphate dehydrogenase (gpdh) were determined in tissue extracts of testes and ovaries of adult dipetalogaster maximus (uhler) and triatoma infestans (klug) (hemiptera: reduviidae), insect vectors of chagas disease. the fine structure organization of the same organs were studied ... | 2001 | 11759815 |
| cuticular hydrocarbons of chagas disease vectors in mexico. | capillary gas-liquid chromatography was used to analyse the cuticular hydrocarbons of three triatomine species, triatoma dimidiata, t. barberi and dipetalogaster maxima, domestic vectors of chagas disease in mexico. mixtures of saturated hydrocarbons of straight and methyl-branched chains were characteristic of the three species, but quantitatively different. major methylbranched components mostly corresponded to different saturated isomers of monomethyl, dimethyl and trimethyl branched hydrocar ... | 2002 | 12386703 |
| infection rates in dipetalogaster maximus (reduviidae: triatominae) by trypanosoma cruzi in the cape region, baja california sur, méxico. | infection rates of trypanosoma cruzi chagas (in the blood-sucking bug dipetalogaster maximus [uhler]) were determined from specimens collected at 12 localities in the cape region of baja california sur, mexico. eight collection sites were located in the tropical dry forest, two in desert shrub, and two in the ecotone between these two communities. of the 245 d. maximus collected, 65% were first and second instar nymphs; 32.6% were third, fourth, and fifth instar nymphs; and 2.4% were adults. the ... | 2003 | 12597648 |
| validation of a minimally invasive blood-sampling technique for the analysis of hormones in domestic rabbits, oryctolagus cuniculus (lagomorpha). | previous studies in small mammals showed that blood-sucking bugs (reduviidae, heteroptera) can be used to obtain blood from veins difficult to access by human experimenters. in the present study, we validated the use of reduviid bugs for endocrinological studies in endotherms using domestic rabbits as a model organism. two processes could alter the hormone concentrations in the blood ingested by the bug: (1) mixing of ingested blood with saliva, gut fluid, or hemolymph and (2) digestive processe ... | 2004 | 14644649 |
| non-invasive blood sampling from primates using laboratory-bred blood-sucking bugs (dipetalogaster maximus; reduviidae, heteroptera). | primates are easily stressed by the conventional veterinary blood sampling routine and consequently, measured blood parameters may be biased. in this study, we tested blood-sucking bugs (dipetalogaster maximus) on one lemur and two ape species (microcebus murinus, pongo abelii, pan paniscus) as an alternative, non-invasive technique for bleeding primates. within time periods of between 6 and 62 min we obtained blood volumes of 0.01-2.4 ml in 11 out of 12 trials from all three species. therefore, ... | 2006 | 16741605 |
| an alternative, less invasive blood sample collection technique for serologic studies utilizing triatomine bugs (heteroptera; insecta). | the collection of blood samples for serological studies is often stressful for the focus animal. recently, the use of bloodsucking bugs, such as dipetalogaster maximus or triatoma infestans (reduviidae; triatominae; heteroptera), has been suggested as a new and less invasive method for blood collection. to evaluate this technique, we collected paired blood samples from 20 domestic rabbits (oryctolagus cuniculus) during a study of rabbit hemorrhagic disease virus (rhdv). for each rabbit, blood sa ... | 2006 | 16870877 |
| vitellogenesis in the hematophagous dipetalogaster maxima (hemiptera: reduviidae), a vector of chagas' disease. | oocyte extracts of anautogenous dipetalogaster maxima were chromatographed on an ion-exchange column in order to purify vitellin (vt), the main insect yolk protein precursor. purified vt (mr ~443 kda) was composed of four subunits with approximate molecular weights of 174, 170, 50, and 44 kda. polyclonal anti-vt antibody, which cross-reacted equally with fat body extracts and hemolymph vitellogenin (vg), was used to measure the kinetics of vg expression in the fat body and the levels in hemolymp ... | 2008 | 18068184 |
| validation of a less invasive blood sampling technique in rabies serology using reduviid bugs (triatominae, hemiptera). | during serologic rabies surveys, bleeding is often difficult or almost impossible in small or endangered mammals such as bats. therefore, the usefulness of an alternative, less invasive technique--that is, the use of blood-sucking reduviid bugs (dipetalogaster maximus and rhodnius prolixus)--was investigated. bugs were used in combination with a conventional method (retro-orbitale bleeding) to obtain blood samples from the same individual nmri-mice (mus musculus) vaccinated against rabies. rabie ... | 2010 | 20722255 |
| dipetalodipin, a novel multifunctional salivary lipocalin that inhibits platelet aggregation, vasoconstriction, and angiogenesis through unique binding specificity for txa2, pgf2alpha, and 15(s)-hete. | dipetalodipin (dptl) is an 18 kda protein cloned from salivary glands of the triatomine dipetalogaster maxima. dptl belongs to the lipocalin superfamily and has strong sequence similarity to pallidipin, a salivary inhibitor of collagen-induced platelet aggregation. dptl expressed in escherichia coli was found to inhibit platelet aggregation by collagen, u-46619, or arachidonic acid without affecting aggregation induced by adp, convulxin, pma, and ristocetin. an assay based on incubation of dptl ... | 2010 | 20889972 |
| insight into the salivary transcriptome and proteome of dipetalogaster maxima. | dipetalogaster maxima is a blood-sucking hemiptera that inhabits sylvatic areas in mexico. it usually takes its blood meal from lizards, but following human population growth, it invaded suburban areas, feeding also on humans and domestic animals. hematophagous insect salivary glands produce potent pharmacologic compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. to obtain further insight into the salivary biochemical and pharmacologic com ... | 2011 | 21058630 |
| biochemical changes in the transition from vitellogenesis to follicular atresia in the hematophagous dipetalogaster maxima (hemiptera: reduviidae). | in this work, we have explored the biochemical changes characterizing the transition from vitellogenesis to follicular atresia, employing the hematophagous insect vector dipetalogaster maxima as a model. standardized insect rearing conditions were established to induce a gradual follicular degeneration stage by depriving females of blood meal during post-vitellogenesis. for the studies, hemolymph and ovaries were sampled at representative days of pre-vitellogenesis, vitellogenesis and early and ... | 2011 | 21763770 |
| salivary antigen-5/cap family members are cu2+-dependent antioxidant enzymes that scavenge o₂₋. and inhibit collagen-induced platelet aggregation and neutrophil oxidative burst. | the function of the antigen-5/cap family of proteins found in the salivary gland of bloodsucking animals has remained elusive for decades. antigen-5 members from the hematophagous insects dipetalogaster maxima (dmav) and triatoma infestans (tiav) were expressed and discovered to attenuate platelet aggregation, atp secretion, and thromboxane a2 generation by low doses of collagen (<1 μg/ml) but no other agonists. dmav did not interact with collagen, glycoprotein vi, or integrin α2β1. this inhibit ... | 2013 | 23564450 |
| the role of dmcatd, a cathepsin d-like peptidase, and acid phosphatase in the process of follicular atresia in dipetalogaster maxima (hemiptera: reduviidae), a vector of chagas' disease. | in this work, we have investigated the involvement of dmcatd, a cathepsin d-like peptidase, and acid phosphatase in the process of follicular atresia of dipetalogaster maxima, a hematophagous insect vector of chagas' disease. for the studies, fat bodies, ovaries and hemolymph were sampled from anautogenous females at representative days of the reproductive cycle: pre-vitellogenesis, vitellogenesis as well as early and late atresia. real time pcr (qpcr) and western blot assays showed that dmcatd ... | 2015 | 26091289 |
| pathological effects of blastocrithidia triatomae (trypanosomatidae) on the reduviid bugs triatoma sordida, t. pallidipennis and dipetalogaster maxima after coprophagic infection. | developmental time and mortality in nymphs of the reduviid bugs triatoma sordida (stål), triatoma pallidipennis (stål) and dipetalogaster maxima (uhler) were studied in uninfected groups and in those infected with blastocrithidia triatomae cerisola et al. (trypanosomatidae). in t.sordida and t.pallidipennis, major vectors of chagas' disease in brazil and mexico respectively, infection with b.triatomae was associated with slight developmental retardations in the final instars, and increased morta ... | 1988 | 2980188 |
| novel adipokinetic hormones in the kissing bugs rhodnius prolixus, triatoma infestans, dipetalogaster maxima and panstrongylus megistus. | peptides of the adipokinetic hormone (akh)/red pigment-concentrating hormone (rpch) family were isolated and sequenced from the retrocerebral corpora cardiaca of four kissing bugs which are all vectors of the protozoan trypanosoma cruzi responsible for chagas' disease. the sequence of three novel akhs were deduced from the multiple ms(n) electrospray mass data: the octapeptide pglu-leu-thr-phe-ser-thr-asp-trp amide (denoted rhopr-akh) in rhodnius prolixus and panstrongylus megistus, the nonapept ... | 2013 | 23137850 |
| pathogenicity of evlachovaea sp (hyphomycetes), a new species isolated from triatoma sordida, in chagas' disease vectors. | evlachovaea sp was tested on nymphs of 5 triatoma spp 5 rhodnius spp, panstrongylus herreri and dipetalogaster maximus at 25 degrees c, 75% humidity and humidity >98%. most species showed susceptibility to fungal infection at high humidity. mortality was reduced at 75% humidity. fungal development was observed on 69.5% of cadavers. | 2006 | 15094910 |
| regulation of breeding behavior: do energy-demanding periods induce a change in prolactin or corticosterone baseline levels in the common tern (sterna hirundo)? | hormones are involved in reproductive decisions, linking environmental cues and body condition and adapting behavior. mass loss is often accompanied by decreased prolactin and increased corticosterone concentrations, influencing incubation and brooding behavior and ultimately triggering nest desertion. using blood-sucking bugs (dipetalogaster maxima), we measured baseline prolactin, corticosterone, and ketone body values in incubating common terns (sterna hirundo) between 2006 and 2009 during en ... | 2014 | 24769706 |
| why do experienced birds reproduce better? possible endocrine mechanisms in a long-lived seabird, the common tern. | the influence of age or breeding experience on reproductive success in vertebrates is well known but physiological mechanisms came into focus just recently. assessing hormone levels could allow insights into these mechanisms and reproductive strategies in long-lived species. prolactin and corticosterone are two hormones involved in breeding decisions: high prolactin values are necessary for expressing breeding behavior whereas corticosterone is related to activity or stress. we analyzed baseline ... | 2012 | 22750538 |
| cell death mechanisms during follicular atresia in dipetalogaster maxima, a vector of chagas' disease (hemiptera: reduviidae). | in this work we have analyzed the involvement of cell death pathways during the process of follicular atresia in the hematophagous insect vector dipetalogaster maxima. standardized insect rearing conditions were established to induce a gradual follicular degeneration stage by depriving females of blood meal during post-vitellogenesis. we first characterized the morpho-histological and ultrastructural changes of the ovarian tissue at early and late follicular atresia by light and transmission ele ... | 2013 | 23500893 |
| [comparison between classical and artificial xenodiagnosis in chronic chagas disease]. | classical and artificial xenodiagnostic techniques made with dipetalogaster maximus of first stage were performed simultaneously in 57 patients with chronic t. cruzi infection (22 male and 35 female patients, aged 7-80 years). with the exception of two patients with megaoesophagus, all had two previous positive serological reaction and a further test was done at the time of the examination. the patients came from the outpatient department of the university hospital or were resident in mambaí, go ... | 2006 | 9789446 |
| dmcatd, a cathepsin d-like peptidase of the hematophagous insect dipetalogaster maxima (hemiptera: reduviidae): purification, bioinformatic analyses and the significance of its interaction with lipophorin in the internalization by developing oocytes. | dmcatd, a cathepsin d-like peptidase of the hematophagous insect dipetalogaster maxima, is synthesized by the fat body and the ovary and functions as yolk protein precursor. functionally, dmcatd is involved in vitellin proteolysis. in this work, we purified and sequenced dmcatd, performed bioinformatic analyses and investigated the events involved in its targeting and storage in developing oocytes. by ion exchange and gel filtration chromatography, dmcatd was purified from egg homogenates and it ... | 2018 | 29325877 |
| the presence of the ancestral insect telomeric motif in kissing bugs (triatominae) rules out the hypothesis of its loss in evolutionarily advanced heteroptera (cimicomorpha). | next-generation sequencing data analysis on triatoma infestans klug, 1834 (heteroptera, cimicomorpha, reduviidae) revealed the presence of the ancestral insect (ttagg)n telomeric motif in its genome. fluorescence in situ hybridization confirms that chromosomes bear this telomeric sequence in their chromosomal ends. furthermore, motif amount estimation was about 0.03% of the total genome, so that the average telomere length in each chromosomal end is almost 18 kb long. we also detected the presen ... | 2016 | 27830050 |
| ovarian nutritional resources during the reproductive cycle of the hematophagous dipetalogaster maxima (hemiptera: reduviidae): focus on lipid metabolism. | in this study, we have analyzed the changes of the ovarian nutritional resources in dipetalogaster maxima at representative days of the reproductive cycle: previtellogenesis, vitellogenesis, as well as fasting-induced early and late atresia. as expected, the amounts of ovarian lipids, proteins, and glycogen increased significantly from previtellogenesis to vitellogenesis and then, diminished during atresia. however, lipids and protein stores found at the atretic stages were higher in comparison ... | 2014 | 25052220 |
| less invasive blood sampling in the animal laboratory: clinical chemistry and haematology of blood obtained by the triatominae bug dipetalogaster maximus. | dipetalogaster maximus (dipmax), a blood-sucking bug belonging to the family reduviidae, has been used to obtain blood samples, for example for clinical chemistry and haematology, in a variety of zoo animals and wildlife. using this bug allows stress-free blood sampling as the bug is able to draw blood without the mammal noticing the bug. in laboratory animal science, the need for blood samples from unstressed animals may arise, especially in animal behaviour research. the use of dipmax bugs may ... | 2012 | 22334876 |
| heme crystallization in the midgut of triatomine insects. | hemozoin (hz) is a heme crystal produced by several blood-feeding organisms in order to detoxify free heme released upon hemoglobin (hb) digestion. here we show that heme crystallization also occurs in three species of triatomine insects. ultraviolet-visible and infrared light absorption spectra of insoluble pigments isolated from the midgut of three triatomine species triatoma infestans, dipetalogaster maximus and panstrongylus megistus indicated that all produce hz. morphological analysis of t ... | 2013 | 17254848 |
| chromogenic substrates as fundamental tool to design new thrombin inhibitors. determination of inhibition equilibrium constants. | the structure-activity relationship of dipetalogastin ii, the strongest thrombin inhibitor isolated and cloned from the bug dipetalogaster maximus, was examined by introducing gradual changes into the molecule by means of molecular biological methods. the effect upon its inhibition equilibrium constant was determined after each change by a chromogenic assay. this structural information was fundamental to design new dipetalogastin ii-derived inhibitors. our results suggested that the acidic seque ... | 2005 | 16113750 |
| three recombinant serine proteinase inhibitors expressed from the coding region of the thrombin inhibitor dipetalogastin. | dipetalogastin is a potent thrombin inhibitor from dipetalogaster maximus. the cdna of dipetalogastin codes for a large protein which consists of six kazal-type domains. there are three tandem, homologous regions each including two domains. three biologically active recombinant proteins rdi, rdii and rdiii each corresponding to one region of the dipetalogastin cdna were expressed, purified and investigated with regard to their biological activities. rdi and rdii with molecular masses of 12,660 a ... | 2004 | 15350616 |
| cloning, purification and biochemical characterization of dipetarudin, a new chimeric thrombin inhibitor. | the development of thrombin inhibitors could provide invaluable progress for antithrombotic therapy. in this paper, we report the cloning, purification and biochemical characterization of dipetarudin, a chimeric thrombin inhibitor composed of the n-terminal head structure of dipetalogastin ii, the strongest inhibitor from the assassin bug dipetalogaster maximus, and the exosite 1 blocking segment of hirudin, connected through a five glycine linker. the cloning of dipetarudin was performed by a s ... | 2003 | 12651003 |
| [correlation among the positivity of the artificial xenodiagnosis and the amount of blood and triatomines used in the exam, in chronic chagasic patients]. | the aim of this study was to verify whether the amount of blood and number of triotomines used could improve the artificial xenodiagnosis performed in 200 chronic phase infected individuals. ten or 40ml of peripheral blood was collected in heparinized (20.4 iu) vacuum tubes, and fed to 60 and 360 triatomines, respectively. dipetalogaster maximus (1st instar, about 15 days after eclosion), as well as 3rd instar triatoma infestans and triatoma vitticeps and the 4th instar of rhodnius neglectus wer ... | 2006 | 11873258 |
| comparison of disturbance stridulations in five species of triatominae bugs. | it has been observed that triatoma infestans and rhodnius prolixus females stridulate to reject copulatory attempts performed by males. in addition, triatomines stridulate when disturbed or handled. in the present study, the temporal structure and frequency spectra of vibrational signals produced by mechanically disturbed t. infestans, t. sordida, t. guasayana, r. prolixus and dipetalogaster maxima were analysed and compared. the inter-ridge distances of the prosternal stridulatory organ of the ... | 2001 | 11369310 |
| biochemical characterization of a thrombin inhibitor from the bloodsucking bug dipetalogaster maximus. | from the bloodsucking bug dipetalogaster maximus, a protein with anticoagulant activity was isolated and biochemically characterized. the isolated protein, named dipetalogastin, possesses an average molecular mass of 11.8 kd. its n-terminal sequence shows homology to rhodniin, a thrombin inhibitor isolated from the bug rhodnius prolixus. the in vitro anticoagulant activity of dipetalogastin occurs via the inhibition of thrombin. the anticoagulant and thrombin inhibitory potency of dipetalogastin ... | 1999 | 10702701 |
| cell growth inhibitor factor in hemolymph of dipetalogaster maximus. | | 2006 | 9566211 |
| [total length of 2 populations of dipetalogaster maximus]. | | 2003 | 7724871 |
| no change in one biological parameter for dipetalogaster maximus after 20 years of laboratory colonisation. | | 2003 | 7724869 |
| resistance of unfed first instar dipetalogaster maximus to different environmental conditions. | | 2003 | 7855371 |
| hatching of eggs of dipetalogaster maximus kept under different climatic conditions. | | 2003 | 7972949 |
| light effect on feeding of first instar dipetalogaster maximus in xenodiagnosis. | | 2004 | 8115688 |
| aspects of classification of hemiptera hemocytes from six triatomine species. | the objective of this work was to characterize, and compare different morphological types of hemocytes of rhodnius prolixus, rhodnius robustus, rhodnius neglectus, triatoma infestans, panstrongylus megistus, and dipetalogaster maximus. this information provides the basis for studying the cellular immune systems of these insects. seven morphological hemocyte types were identified by phase-contrast microscopy: prohemocytes, plasmatocytes, granular cells, cystocytes, oenocytoids, adipohemocytes and ... | 2006 | 1842390 |
| the influence of building materials on the residual action of bhc. | residual insecticide activity of bhc vapors from various building materials in controlled humidity chambers in the laboratory were significantly different. laboratory-reared, first instar nymphs of dipetalogaster maximus were exposed to vapors of bhc which were being released from the treated surfaces of building materials taken from mambaí, goiás. | 2013 | 2427913 |
| [biological aspects and large scale cultivation of dipetalogaster maximus (uhler, 1894) (triatominae)]. | | 2006 | 7025164 |
| skin reactions to bug bites as a result of xenodiagnosis. | cutaneous allergic reactions to bites of two bug species triatoma infestans and dipetalogaster maxima, were studied in an area where t. infestans is the local domestic vector. while more delayed skin reactions occurred with the indigenous vector, reactions also occurred with d. maxima. repeated exposure to d. maxima produced more intense immediate skin reactions. first-stage d. maxima bugs produced a lower frequency and intensity of skin reaction. it is concluded that further work on this proble ... | 1981 | 6798721 |
| [absence of perceptible odoriferous emanations in dipetalogaster maximus. some comments on this species]. | | 2003 | 5499566 |