| bacillus mojavensis sp. nov., distinguishable from bacillus subtilis by sexual isolation, divergence in dna sequence, and differences in fatty acid composition. | a number of bacillus strains isolated from desert soil samples were shown to belong to a previously unidentified species, for which we propose the name bacillus mojavensis. the type strain is ro-h-1 (= nrrl b-14698). on the basis of restriction digest data, b. mojavensis is most closely related to bacillus amyloliquefaciens, bacillus atrophaeus, and bacillus subtilis. so far, b. mojavensis can be distinguished from b. subtilis only by differences in whole-cell fatty acid composition, divergence ... | 1994 | 8186089 |
| related homing endonucleases i-bmoi and i-tevi use different strategies to cleave homologous recognition sites. | a typical homing endonuclease initiates mobility of its group i intron by recognizing dna both upstream and downstream of the intron insertion site of intronless alleles, preventing the endonuclease from binding and cleaving its own intron-containing allele. here, we describe a giy-yig family homing endonuclease, i-bmoi, that possesses an unusual recognition sequence, encompassing 1 base pair upstream but 38 base pairs downstream of the intron insertion site. i-bmoi binds intron-containing and i ... | 2001 | 11416170 |
| kinetic constants determination for an alkaline protease from bacillus mojavensis using response surface methodology. | the kinetic constants for an alkaline protease from bacillus mojavensis were determined using a central composite circumscribed design (cccd) where concentration of substrate (casein) and the assay temperature were varied around their center point. the k(m),v(max), k(cat), activation energy (e(a)) and temperature coefficient (q(10)) were determined and the values of these kinetic constants obtained were found comparable to that obtained with conventional methods. the michaelis-menten constant (k ... | 2002 | 11920445 |
| isolation and characterization of a novel bacillus strain from coffee phyllosphere showing antifungal activity. | the isolation and characterization of a novel coffee-associated bacillus mojavensis strain, designated as strain ab1, and its survival on the coffee phyllosphere. | 2002 | 12392522 |
| phylogenetic relationships between bacillus species and related genera inferred from comparison of 3' end 16s rdna and 5' end 16s-23s its nucleotide sequences. | the nucleotide sequences of the 3' end of the 16s rdna and the 16s-23s internal transcribed spacer (its) of 40 bacillaceae species were determined. these included 21 bacillus, 9 paenibacillus, 6 brevibacillus, 2 geobacillus, 1 marinibacillus and 1 virgibacillus species. comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16s rrna coding region and a conserved 70 bp sequence located at the 5' end of the 16s-23s its of the 40 sp ... | 2003 | 12807189 |
| anaerobic growth of bacillus mojavensis and bacillus subtilis requires deoxyribonucleosides or dna. | bacillus mojavensis strains jf-2 (atcc 39307), rob2, and abo21191(t) and bacillus subtilis strains 168 (atcc 23857) and atcc 12332 required four deoxyribonucleosides or dna for growth under strict anaerobic conditions. bacillus licheniformis strains l89-11 and l87-11, bacillus sonorensis strain tg8-8, and bacillus cereus (atcc 14579) did not require dna for anaerobic growth. the requirement for the deoxyribonucleosides or dna did not occur under aerobic growth conditions. the addition of a mixtu ... | 2004 | 15345407 |
| bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river vélez in málaga, southern spain. | two gram-positive, endospore-forming bacterial strains, cr-502t and cr-14b, which produce surfactant molecules are described. phenotypic tests and phylogenetic analyses showed these strains to be members of the genus bacillus and related to the species bacillus atrophaeus, bacillus mojavensis, bacillus subtilis, bacillus vallismortis and bacillus amyloliquefaciens, although they differ from these species in a number of phenotypic characteristics. dna-dna hybridization confirmed that they show le ... | 2005 | 15653875 |
| two self-splicing group i introns interrupt two late transcribed genes of prolate-headed lactobacillus delbrueckii phage jcl1032. | two group i introns were detected from the late gene region of the prolate-headed phage jcl1032 of lactobacillus delbrueckii. introns jcl-i1 and jcl-i2 interrupt orf602 and orf1868 encoding a phage terminase large subunit (terl, 69.7 kda) and a putative tape measure protein (tmp, 202 kda), respectively. the introns jcl-i1 (226 bp) and jcl-i2 (322 bp) were efficiently self-spliced in vivo. both introns were classified to the subgroup ia1 having all the conserved structures necessary for splicing, ... | 2004 | 15669111 |
| bacillus axarquiensis sp. nov. and bacillus malacitensis sp. nov., isolated from river-mouth sediments in southern spain. | two gram-positive, rod-shaped, endospore-forming bacteria (strains cr-119(t) and cr-95(t)) were isolated from brackish sediments in the mouth of the river velez in malaga, southern spain, and subjected to a polyphasic taxonomic study. phenotypic tests showed that these strains were related to other bacillus species at a similarity level of less than 87.6 %. both strains are halotolerant, aerobic, chemoheterotrophic, motile with peritrichous flagella and biosurfactant producers. their endospores ... | 2005 | 15879268 |
| importance of 3-hydroxy fatty acid composition of lipopeptides for biosurfactant activity. | biosurfactant production may be an economic approach to improving oil recovery. to obtain candidates most suitable for oil recovery, 207 strains, mostly belonging to the genus bacillus, were tested for growth and biosurfactant production in medium with 5% nacl under aerobic and anaerobic conditions. all strains grew aerobically with 5% nacl, and 147 strains produced a biosurfactant. thirty-five strains grew anaerobically with 5% nacl, and two produced a biosurfactant. in order to relate structur ... | 2005 | 16332741 |
| growth-inhibiting effects of concentrations of fusaric acid on the growth of bacillus mojavensis and other biocontrol bacillus species. | to determine the effects of concentrations of fusaric acid on the growth of several strains of the biocontrol bacterial endophyte bacillus mojavensis and other species within the bacillus subtilis group, as well as the genetic relationships within this small group of gram-positive bacteria, and their antagonisms to fusarium verticillioides, which produce fusaric acid. | 2006 | 16405699 |
| re-identification of the halotolerant, biosurfactant-producing bacillus licheniformis strain jf-2 as bacillus mojavensis strain jf-2. | bacillus strain jf-2 (atcc 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species bacillus licheniformis based on a limited set of phenotypic characteristics. here, genetic and phenotypic analyses were employed to determine the relationship of bacillus strain jf-2 to other bacillus strains. the restriction patterns with alui and analysis of gyra and 16s rrna gene sequences grouped bacillus strain jf-2 with b. mojavensis(t) and not with ... | 2006 | 16488097 |
| small rolling circle plasmids in bacillus subtilis and related species: organization, distribution, and their possible role in host physiology. | bacillus subtilis and related species (bacillus licheniformis, bacillus pumilus, bacillus amyloliquefaciens, and bacillus mojavensis) represent a group of bacteria largely studied and widely employed by industry. small rolling circle replicating plasmids of this group of bacteria have been intensively studied as they represent a convenient model for genetic research and for the construction of molecular tools for the genetic modification of their hosts. through the computational analysis of the ... | 2007 | 17064773 |
| cytotoxicity in bacillus mojavensis is abolished following loss of surfactin synthesis: implications for assessment of toxicity and food poisoning potential. | bacillus subtilis and the closely related species bacillus pumilus and bacillus licheniformis have periodically been suggested to play a role in the aetiology of food poisoning despite the fact that the organisms do not possess the genes associated with enteropathogenicity in bacillus cereus. we show here that bacillus mojavensis, an organism closely related to b. subtilis, is able to produce toxic components which identify as a complex of three different surfactin analogues. these cyclic lipope ... | 2007 | 17467096 |
| acid labile comx pheromone from bacillus mojavensis ro-h-1. | bacillus mojavensis strain ro-h-1 produces a posttranslationally modified hexapeptide, the comx(ro-h-1) pheromone, that stimulates natural genetic competence controlled by quorum sensing. lc/esi-ms analysis of partially purification of the comx(ro-h-1) pheromone suggested a precise modification in its tryptophan residue. the corresponding comx(ro-h-1) pheromone prepared by solid-phase synthesis was identical to the natural pheromone, and showed significant biological activity. these results indi ... | 2007 | 17617699 |
| reclassification of bacillus axarquiensis ruiz-garcia et al. 2005 and bacillus malacitensis ruiz-garcia et al. 2005 as later heterotypic synonyms of bacillus mojavensis roberts et al. 1994. | the bacillus subtilis group encompasses the taxa bacillus subtilis subsp. subtilis, b. licheniformis, b. amyloliquefaciens, b. atrophaeus, b. mojavensis, b. vallismortis, b. subtilis subsp. spizizenii, b. sonorensis, b. velezensis, b. axarquiensis and b. malacitensis. in this study, the taxonomic relatedness between the species b. axarquiensis, b. malacitensis and b. mojavensis was investigated. sequence analysis of the 16s rrna gene and the gene for dna gyrase subunit b (gyrb) confirmed the ver ... | 2007 | 17625213 |
| interactions of bacillus mojavensis and fusarium verticillioides with a benzoxazolinone (boa) and its transformation product, apo. | the benzoxazolinones, specifically benzoxazolin-2(3h)-one (boa), are important transformation products of the benzoxazinones that can serve as allelochemicals providing resistance to maize from pathogenic bacteria, fungi, and insects. however, maize pathogens such as fusarium verticillioides are capable of detoxifying the benzoxazolinones to 2-aminophenol (ap), which is converted to the less toxic n-(2-hydroxyphenyl) malonamic acid (hpma) and 2-acetamidophenol (hpaa). as biocontrol strategies th ... | 2007 | 17896139 |
| antifouling activity of sessile bacilli derived from marine surfaces. | marine biofilms are a virtually untapped source of bioactive molecules that may find application as novel antifoulants in the marine paint industry. this study aimed at determining the potential of marine biofilm bacteria to produce novel biomolecules with potential application as natural antifoulants. nine representative strains were isolated from a range of surfaces and were grown in yeb medium and harvested during the late exponential growth phase. bacterial biomass and spent culture medium w ... | 2008 | 17909869 |
| csf, a species-specific extracellular signaling peptide for communication among strains of bacillus subtilis and bacillus mojavensis. | comx and csf are bacillus subtilis extracellular signaling peptides. many different strains of b. subtilis do not communicate due to strain-specific variation of comx. we demonstrate that csf is a species-specific signaling molecule that partially compensates for the lack of comx-mediated communication between different strains of b. subtilis. | 2008 | 18375560 |
| electrotransformation of bacillus mojavensis with fluorescent protein markers. | gram-positive endophytic bacteria are difficult to transform. to study the interactions between bacillus mojavensis and maize, a method was developed to transform the microbe by electroporation with three integration plasmids expressing green, cyan and yellow fluorescent proteins (gfp variants). gfp transformations were verified by antibiotic selection, microscopy and amylase deficiency, based on incorporation of the plasmids through recombination with the amylase gene. phenotypic expressions of ... | 2008 | 18486251 |
| evaluation of bioemulsifier mediated microbial enhanced oil recovery using sand pack column. | bacillus licheniformis k125, isolated from an oil reservoir, produces an effective bioemulsifier. the crude bioemulsifier showed 66% emulsification activity (e(24)) and reduced the surface tension of water from 72 to 34 mn/m. it contains substantial amount of polysaccharide, protein and lipid. this bioemulsifier is pseudoplastic non-newtonian in nature. it forms oil in water emulsion which remains stable at wide range of ph, temperature and salinity. it gave 43+/-3.3% additional oil recovery upo ... | 2008 | 18625271 |
| antagonism of bacillus spp. isolated from marine biofilms against terrestrial phytopathogenic fungi. | we aimed at determining the antagonistic behavior of bacteria derived from marine biofilms against terrestrial phytopathogenic fungi. some bacteria closely related to bacillus mojavensis (three isolates) and bacillus firmus (one isolate) displayed antagonistic activity against colletotrichum gloeosporioides atcc 42374, selected as first screen organism. the four isolates were further quantitatively tested against c. gloeosporioides, colletotrichum fragariae, and fusarium oxysporum on two culture ... | 2009 | 18931878 |
| isolation, purification and characterization of a surfactants-, laundry detergents- and organic solvents-resistant alkaline protease from bacillus sp. hr-08. | bacillus sp. hr-08 screened from soil samples of iran, is capable of producing proteolytic enzymes. 16s rdna analysis showed that this strain is closely related to bacillus subtilis, bacillus licheniformis, bacillus pumilus, bacillus mojavensis, and bacillus atrophaeus. the zymogram analysis of the crude extract revealed the presence of five extracellular proteases. one of the proteases was purified in three steps procedure involving ammonium sulfate precipitation, deae-sepharose ionic exchange ... | 2009 | 19148779 |
| effects of dietary probiotic supplementations on prevention/treatment of yersiniosis disease. | to evaluate enterobacter cloacae and bacillus mojavensis, isolated from rainbow trout gut in the present study, as a probiotic to control yersiniosis disease. | 2009 | 19191963 |
| two detergent stable alkaline serine-proteases from bacillus mojavensis a21: purification, characterization and potential application as a laundry detergent additive. | two detergent stable alkaline serine-proteases (bm1 and bm2) from bacillus mojavensis a21 were purified. the molecular weights of bm1 and bm2 enzymes determined by sds-page were approximately 29,00 da and 15,50 da, respectively. the optimum ph values of bm1 and bm2 proteases were shown to be 8.0-10.0 and 10.0, respectively. both enzymes exhibited maximal activity at 60 degrees c, using casein as a substrate. the n-terminal amino acid sequences of bm1 and bm2 proteases were aqsvpygisqika and aipd ... | 2009 | 19269812 |
| isolation and characterization of leu7-surfactin from the endophytic bacterium bacillus mojavensis rrc 101, a biocontrol agent for fusarium verticillioides. | bacillus mojavensis is an endophytic bacterium patented for control of fungal diseases in maize and other plants. culture extracts and filtrates from this bacterium were antagonistic to the pathogenic and mycotoxic fungus fusarium verticillioides. however, the identity of the inhibitory substance from extracts of this bacterium has not been determined. an hplc-ms analysis of the culture filtrate showed a major ion peak that was identified as a cyclic lipopeptide. furthermore, collisional ion dis ... | 2009 | 19371139 |
| bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in thailand. | a gram-positive, endospore-forming, rod-shaped bacterium, strain pd-a10(t), was isolated from salted crab (poo-khem) in thailand and subjected to a taxonomic study. phenotypic and chemotaxonomic characteristics, including phylogenetic analyses, showed that the novel strain was a member of the genus bacillus. the novel strain grew in medium with 0-14 % (w/v) nacl, at 4-55°c and at ph4.5-9. the predominant quinone was a menaquinone with seven isoprene units (mk-7). the major fatty acids were antei ... | 2010 | 19933584 |
| bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil. | a gram-positive bacterium, designated strain cbmb205(t), was isolated from the rhizosphere soil of traditionally cultivated, field-grown rice. cells were strictly aerobic, motile, rod-shaped and formed endospores. the best growth was achieved at 30°c and ph 7.0 in ammonium mineral salts (ams) medium containing 600 mm methanol. a comparative 16s rrna gene sequence-based phylogenetic analysis placed strain cbmb205(t) in a clade with the species bacillus amyloliquefaciens, bacillus vallismortis, ba ... | 2010 | 19966000 |
| a novel alpha-amylase from bacillus mojavensis a21: purification and biochemical characterization. | alpha-amylase from bacillus mojavensis a21 (bma.2) was purified to homogeneity by ultrafiltration, sephadex g-75 gel filtration and sepharose mono q anion exchange chromatography, with a 15.3-fold increase in specific activity and 11% recovery. the molecular weight of the bma.2 enzyme was estimated to be 58 kda by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and gel filtration. the optimum temperature and ph were 80 degrees c and 6.5, respectively. bma.2 belonged to the ... | 2010 | 20108054 |
| analysis of novel angiotensin i-converting enzyme inhibitory peptides from enzymatic hydrolysates of cuttlefish (sepia officinalis) muscle proteins. | the angiotensin i-converting enzyme (ace) inhibitory activities of protein hydrolysates prepared from cuttlefish (sepia officinalis) proteins by treatment with various bacterial proteases were investigated. the hydrolysate generated by the crude enzyme from bacillus mojavensis a21 displayed the highest ace inhibitory activity, and the higher inhibition activity (87.11 +/- 0.92% at 2 mg/ml) was obtained with hydrolysis degree of 16%. this hydrolysate was fractionated by size exclusion chromatogra ... | 2010 | 20180574 |
| low-cost fermentation medium for alkaline protease production by bacillus mojavensis a21 using hulled grain of wheat and sardinella peptone. | media composition and culture conditions for surfactant stable alkaline protease production by bacillus mojavensis a21 were optimized using two statistical methods. plackett-burman design was applied to find the optimal ingredients and conditions to improve yields. response surface methodology (rsm), including central composite design, was used to determine the optimal concentrations and conditions. the results indicated that several components, including hulled grain of wheat (hgw), sardinella ... | 2010 | 20547353 |
| characterization of bacillus mojavensis kjs-3 for industrial applications. | one of the strains of the bacillus mojavensis group, bacillus mojavensis kjs-3 (b. mojavensis kjs-3), which has been demonstrated to play a role in protecting plants against diseases as a bacterial endophyte and in reducing the accumulation of mycotoxins generated by an endophytic fungus, was recently discovered in food waste. in this study, the identification and characterization of b. mojavensis kjs-3 was performed via tem analysis, api-zym test, api 50 chb test, assays of catalase and oxidase ... | 2011 | 21380813 |
| in planta reduction of maize seedling stalk lesions by the bacterial endophyte bacillus mojavensis. | maize ( zea mays l.) is susceptible to infection by fusarium verticillioides through autoinfection and alloinfection, resulting in diseases and contamination of maize kernels with the fumonisin mycotoxins. attempts at controlling this fungus are currently being done with biocontrol agents such as bacteria, and this includes bacterial endophytes, such as bacillus mojavensis . in addition to producing fumonisins, which are phytotoxic and mycotoxic, f. verticillioides also produces fusaric acid, wh ... | 2011 | 21635192 |
| bacillus mojavensis strain 32a, a bioflocculant-producing bacterium isolated from an egyptian salt production pond. | bacillus mojavensis strain 32a that exhibited 96.11% flocculation efficiency for clay suspensions was selected from other 15 comparative strains. under growth condition, strain 32a was able to produce 5.2g/l of purified biopolymer. its constituent was mainly polysaccharide and protein with proportional of 98.4-1.6% respectively. ftir spectrum was confirming its chemical analysis. this biopolymer attain very fast sedimentation rate. the cost-effective biopolymer and cacl(2) dosages were 3mg/l and ... | 2011 | 21689928 |
| Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste. | A xylanase producer Bacillus mojavensis strain, called AG137, isolated from cotton farm (Kashan-Iran). The optimal xylanase activity reached at 55°C & pH 9.0. Enzyme yield was studied using a medium with different agricultural wastes as inducers. Xylanase production of about 249.308?IU/mL was achieved at pH 8 and 37°C, within 48?h submerged fermentation in enzyme production medium supplemented with 2% (w/v) oat bran as an optimum carbon source. A mixture of 1% (w/v) yeast extract and 1% (w/v) tr ... | 2011 | 21904670 |
| whole-genome shotgun sequence of bacillus mojavensis strain rrc101, an endophytic bacterium antagonistic to the mycotoxigenic endophytic fungus fusarium verticillioides. | here, we report the whole-genome shotgun sequence of bacillus mojavensis strain rrc101, isolated from a maize kernel. this strain is antagonistic to the mycotoxigenic plant pathogen fusarium verticillioides and grows within maize tissue, suggesting potential as an endophytic biocontrol agent. | 2014 | 25359909 |
| selection of bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets. | we have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. the spore-forming bacillus species are widely used as probiotics in feed supplements for pigs. in this study, we evaluated the option for further functionalizing bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. we established ... | 2017 | 28204884 |
| chitin extraction from crab shells by bacillus bacteria. biological activities of fermented crab supernatants. | crab shells waste were fermented using six protease-producing bacillus species (bacillus subtilis a26, bacillus mojavensis a21, bacillus pumilus a1, bacillus amyloliquefaciens an6, bacillus licheniformis nh1 and bacillus cereus bg1) for the production of chitin and fermented-crab supernatants (fcss). in medium containing only crab shells, the highest demineralization dm was obtained with b. licheniformis nh1 (83±0.5%) and b. pumilus a1 (80±0.6%), while the highest deproteinization (dp) was achie ... | 2015 | 25910648 |
| isolation and characterization of putative endophytic bacteria antagonistic to phoma tracheiphila and verticillium albo-atrum. | a collection of 200 bacterial isolates recovered from citrus plants (citrus limon, citrus sinensis, and citrus reticulata), medicago truncatula and laurus nobilis, was established. in vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens phoma tracheiphila and verticillium albo-atrum. isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (pgpb) abilities, as well as for the presence of nonribosomal pe ... | 2014 | 25074353 |
| [teichoic acids of three type strains of the "bacillus subtilis" group, bacillus mojavensis vkm b-2650, bacillus amyloliquefaciens subsp. amyloliquefaciens vkm b-2582, and bacillus sonorensis vkm b-2652]. | cell walls of three type strains of the "bacillus subtilis" group, bacillus mojavensis vkm b-2650, bacillus amyloliquefaciens subsp. amyloliquefaciens vkm b-2582, and bacillus sonorensis vkm b-2652, are characterized by the individual set of teichoic acids. all strains contained 1,3-poly(glycerophosphates), unsubstituted, acylated with d-alanine, and glycosylated. the latter differ in the nature of the monosaccharide residue. teichoic acids of b. mojavensis vkm b-2650(t) and b. amyloliquefaciens ... | 2014 | 25509395 |
| [screening and identification of low temperature-adapted antagonistic bacillus isolated from kekexili region of west china and the analysis of the isolates lipopeptide compounds]. | the research and exploitation of special microbial resources in extreme environment is of scientific significance and has broad applied prospect. in this paper, eight bacillus strains isolated from the vegetation rhizospheres in kekexili extreme region of qinghai province and presented good growth status at low temperature 4 and 10 degrees c were identified. through physiological and biochemical analysis, rep-pcr fingerprinting, and 16s rdna and gyrb partial sequence analyses, the eight strains ... | 2013 | 23718003 |
| screening of novel bacteria for the 2,3-butanediol production. | biotechnologically produced 2,3-butanediol (2,3-bdo) is a potential starting material for industrial bulk chemicals such as butadiene or methyl ethyl ketone which are currently produced from fossil feedstocks. so far, the highest 2,3-bdo concentrations have been obtained with risk group 2 microorganisms. in this study, three risk group 1 microorganisms are presented that are so far unknown for an efficient production of 2,3-bdo. the strains bacillus atrophaeus nrs-213, bacillus mojavensis b-1469 ... | 2017 | 27687995 |
| genome analysis shows bacillus axarquiensis is not a later heterotypic synonym of bacillus mojavensis; reclassification of bacillus malacitensis and brevibacterium halotolerans as heterotypic synonyms of bacillus axarquiensis. | bacillus axarquiensis and bacillus malacitensis were previously reported to be later heterotypic synonyms of bacillus mojavensis, based primarily on dna-dna relatedness values. we have sequenced draft genomes of bacillus axarquiensis nrrl b-41617t and bacillus malacitensis nrrl b-41618t. comparative genomics and dna-dna relatedness calculations showed that while bacillus axarquiensis and bacillus malacitensis are synonymous with each other, they are not synonymous with bacillus mojavensis. in ad ... | 2016 | 27030978 |
| toxigenic potential and heat survival of spore-forming bacteria isolated from bread and ingredients. | fifty-four spore-forming bacterial strains isolated from bread ingredients and bread, mainly belonging to the genus bacillus (including bacillus cereus), together with 11 reference strains were investigated to evaluate their cytotoxic potential and heat survival in order to ascertain if they could represent a risk for consumer health. therefore, we performed a screening test of cytotoxic activity on ht-29 cells using bacterial culture filtrates after growing bacterial cells in brain heart infusi ... | 2015 | 25555227 |
| characterization of biosurfactant produced by bacillus licheniformis tt42 having potential for enhanced oil recovery. | bacillus licheniformis tt42 produced a low-molecular weight anionic biosurfactant that reduced the surface tension of water from 72 to 27 mn/m and the interfacial tension from 12 to 0.05 mn/m against crude oil. we have earlier reported significant enhancement in oil recovery in laboratory sand pack columns and core flood studies, by biosurfactant-tt42 compared to standard strain, bacillus mojavensis jf2. in the context of this application of the biosurfactant-tt42, its characterization was deeme ... | 2016 | 27131876 |
| characterization and potential use of cuttlefish skin gelatin hydrolysates prepared by different microbial proteases. | composition, functional properties, and in vitro antioxidant activities of gelatin hydrolysates prepared from cuttlefish skin were investigated. cuttlefish skin gelatin hydrolysates (csghs) were obtained by treatment with crude enzyme preparations from bacillus licheniformis nh1, bacillus mojavensis a21, bacillus subtilis a26, and commercial alcalase. all csghs had high protein contents, 74.3-78.3%, and showed excellent solubility (over 90%). csgh obtained by alcalase demonstrated high antioxida ... | 2014 | 25025053 |
| antimicrobial activity and chemical composition of three essential oils extracted from mediterranean aromatic plants. | there is a growing interest in essential oils (eos) as possible alternatives for traditional chemical pesticides. this study was carried out to characterize the chemical composition of the three eos extracted from verbena officinalis, majorana hortensis, and salvia officinalis using gas chromatography (gc) and gc-mass spectrometry (gc-ms) and to evaluate in vitro their efficacy against some phyto or human pathogens. the antifungal activity was investigated against colletotrichum acutatum and bot ... | 2016 | 27792456 |
| effects of different heat treatments on lysozyme quantity and antimicrobial activity of jenny milk. | thermal treatments are used to improve milk microbial safety, shelf life, and biological activity of some of its components. however, thermal treatments can reduce the nutritional quality of milk, affecting the molecular structure of milk proteins, such as lysozyme, which is a very important milk component due to its antimicrobial effect against gram-positive bacteria. jenny milk is characterized by high lysozyme content. for this reason, in the last few years, it has been used as an antimicrobi ... | 2016 | 27157571 |
| an in vitro attempt for controlling severe phytopathogens and human pathogens using essential oils from mediterranean plants of genus schinus. | growing concerns about food safety and environmental protection enhanced the need for new and safe plant disease control strategies. the chemical composition of the three essential oils (eos) extracted from leaves and fruits of schinus terebinthifolius and leaves of schinus molle, growing in tunisia, was studied by gc and gc-ms. in all, 12 compounds were identified. the oils were mainly composed of terpene compounds. α-pinene, α-phellandrene, and d-limonene were the major constituents. the aim o ... | 2016 | 26836214 |
| tolerance and bioaccumulation of u(vi) by bacillus mojavensis and its solid phase preconcentration by bacillus mojavensis immobilized multiwalled carbon nanotube. | in this study, uranium(vi) tolerance and bioaccumulation were investigated by using thermo -tolerant bacillus mojavensis. the level of u(vi) was measured by uv-vis spectrophotometry. the minimum inhibition concentration (mic) value of u(vi) was experimented. bacterial growth was not affected in the presence of 1.0 and 2.5 mg/l u(vi) at 36 h and the growth was partially affected in the presence of 5 mg/l u(vi) at 24 h. what was obtained from this study is that there was diversity in the various p ... | 2017 | 27856038 |
| bacillus mojavensis rrc101 lipopeptides provoke physiological and metabolic changes during antagonism against fusarium verticillioides. | the mycotoxigenic pathogen fusarium verticillioides threatens the quality and utility of maize across industrial and agricultural purposes. chemical control is complicated by the intimate endophytic lifestyle of the pathogen with its host. bacillus mojavensis rrc101, a maize-endophytic bacterium, has been observed to reduce f. verticillioides disease severity and fumonisin accumulation when coinoculated to maize. genome sequencing and annotation identified a number of biocontrol-relevant pathway ... | 2016 | 27537213 |
| enrichment and identification of biosurfactant-producing oil field microbiota utilizing electron acceptors other than oxygen and nitrate. | microorganisms indigenous to an oil reservoir were grown in media containing either sucrose or proteins in four steel vessels under anoxic conditions at 30°c and 8.3mpa for 30days, to enrich biosurfactant producers. fermentation of substrate was possible in the protein-containing medium and either fermentation or respiration through reduction of sulfate occurred in the sucrose-containing medium. growth of microorganisms led to 3.4-5.4-fold surface tension reduction indicating production of biosu ... | 2016 | 27212608 |
| isolation and characterization of bacteria with antibacterial properties from nile tilapia (oreochromis niloticus). | one hundred and twenty bacterial isolates were obtained from the intestinal mucus of nile tilapia (oreochromis niloticus) and screened for antagonistic activity and adherence abilities. based on in vitro antagonism against two pathogens (streptococcus iniae and edwardsiella piscicida), five isolates were selected and identified by 16s rrna gene sequence analysis. all antagonistic isolates were affiliated to the genus bacillus, which showed inhibitory activity against s. iniae. only the isolate b ... | 2016 | 27033910 |
| identification of endophytic bacillus mojavensis with highly specialized broad spectrum antibacterial activity. | biosynthetic adaptation of endophytic bacteria to chemically support host plant is very remarkable. hence these organisms from medicinal plants are considered as highly valuable sources for natural products with diverse bioactivity. their metabolite diversity and biosynthetic versatility have been increasingly explored for drug discovery. in this study, an endophytic bacillus mojavensis with broad spectrum antibacterial properties has been analyzed for the chemical basis of its activity. by lc-m ... | 2016 | 28330259 |
| assessment of pectinase production by bacillus mojavensis i4 using an economical substrate and its potential application in oil sesame extraction. | carrot (daucus carota) peels, local agricultural waste product, is rich in lignocellulolytic material, including pectin which can act as an inducer of pectinase production. pectinolytic enzymes production by bacillus mojavensis i4 was studied in liquid state fermentation using carrot peel as a substrate. medium composition and culture conditions for the pectinase production by i4 were optimized using two statistical methods: taguchi design was applied to find the key ingredients and conditions f ... | 2015 | 26604345 |
| virgibacillus proomii and bacillus mojavensis as probiotics in sea bass (dicentrarchus labrax) larvae: effects on growth performance and digestive enzyme activities. | this study examined the effects of two probiotics (virgibacillus proomii and bacillus mojavensis) on the digestive enzyme activity, survival and growth of dicentrarchus labrax at various ontogenetic stages in three separate experiments. these probiotics were incorporated as single or mixed into fish feed for a period of 60 days. the growth parameters, proximate composition of whole body, digestive enzymes and gut microbiology were monitored at regular. the increments in length and weight and the ... | 2016 | 26520833 |
| production and characterization of surfactin-type lipopeptides as bioemulsifiers produced by a pinctada martensii-derived bacillus mojavensis b0621a. | bacillus mojavensis b0621a was isolated from the mantle of a pearl oyster pinctada martensii collected from south china sea. semi-purified surfactins (225 mg l(-1)) were obtained by acid precipitation and vacuum flash chromatography. the component of the semi-purified surfactins was preliminarily analyzed by liquid chromatography mass spectrometer system, and the results showed that all these surfactins could be a group of homologues. eight surfactin homologues were isolated and afforded by reve ... | 2015 | 26373943 |
| optimization of proteins and minerals removal from shrimp shells to produce highly acetylated chitin. | chitin and derivatives used for biomedical or pharmaceutical applications require a high level of purity and quality that are difficult to achieve. in this study, we propose to optimize the extraction of chitin in order to obtain pure product keeping a structure as close as possible to the native form. thus, demineralization step was firstly optimized using response surface methodology. in the optimized conditions predicted by the model, the obtained chitin has an acetylation degree (da) and a d ... | 2016 | 26299708 |
| acute and sub-chronic oral toxicity profiles of lipopeptides from bacillus mojavensis a21 and evaluation of their in vitro anticoagulant activity. | the aim of the present study was to evaluate the acute and sub-chronic toxicity of lipopeptides mixture produced by bacillus mojavensis a21 as well as their in vitro anticoagulant activity. a21 lipopeptides was given to mice at single dose from 75 mg to 1000 mg/kg body weight (bw). the median lethal dose (ld50) of a21 lipopeptides was about 550 mg/kg bw. sub-chronic toxicity study for 28 days was done by daily oral administration of a21 lipopeptides at doses of 40 and 400 mg/kg bw in rats. resul ... | 2015 | 25912554 |
| isolation and characterisation of bacillus spp. antagonistic to vibrio parahaemolyticus for use as probiotics in aquaculture. | acute gastroenteritis caused by pathogenic vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. in this study, a total of 249 isolates were isolated from four types of seafood (litopenaeus vannamei, oratosquilla oratoria, mactra veneriformis and portunus trituberculatus) and ... | 2015 | 25737203 |
| uv mutagenesis for the overproduction of xylanase from bacillus mojavensis ptcc 1723 and optimization of the production condition. | this study highlights xylanase overproduction from bacillus mojavensis via uv mutagenesis and optimization of the production process. | 2014 | 25691925 |
| biological activities of xylooligosaccharides generated from garlic straw xylan by purified xylanase from bacillus mojavensis ueb-fk. | a newly isolated bacterium strain named ueb-fk was selected from tunisian sahara, exhibiting the highest clear zone on agar plates containing oat spelt xylan by staining with congo red. on the basis of 16s rdna sequence analysis, this strain was identified as bacillus mojavensis. this strain produced extracellular xylanase. xylanase from the strain was purified to homogeneity and had an apparent molecular weight of 14 kda. the k m and v max values of the purified xylanase on oat spelt xylan were ... | 2015 | 25351626 |
| chitin extraction from shrimp shell using enzymatic treatment. antitumor, antioxidant and antimicrobial activities of chitosan. | chitin was recovered through enzymatic deproteinization of the shrimp processing by-products. different microbial and fish viscera proteases were tested for their deproteinization efficiency. high levels of protein removal of about 77±3% and 78±2% were recorded using bacillus mojavensis a21 and balistes capriscus proteases, respectively, after 3h of hydrolysis at 45°c using an enzyme/substrate ratio of 20u/mg. therefore, these two crude proteases were used separately for chitin extraction and th ... | 2014 | 24950313 |
| composition, functional properties and in vitro antioxidant activity of protein hydrolysates prepared from sardinelle (sardinella aurita) muscle. | composition, functional properties and in vitro antioxidative activities of protein hydrolysates prepared from muscle of sardinelle (sardinella aurita) were investigated. sardinelle protein hydrolysates (sph) were obtained by treatment with crude enzyme preparations from bacillus pumilus a1 (spha1), bacillus mojavensis a21 (spha21) and crude enzyme extract from sardinelle (sardinella aurita) viscera (sphee). the protein hydrolysates spha1, spha21 and sphee contained high protein content 79.1%, 7 ... | 2014 | 24741155 |
| production and characterization of iturinic lipopeptides as antifungal agents and biosurfactants produced by a marine pinctada martensii-derived bacillus mojavensis b0621a. | bacillus mojavensis b0621a was isolated from a pearl oyster pinctada martensii collected from south china sea. while screening for cyclic lipopeptides potentially useful as lead compounds for biological control against soil-bone fungal plant pathogens, three lipopeptides were isolated and purified from the fermentation broth of b. mojavensis b0621a via vacuum flash chromatography coupled with reversed-phase high performance liquid chromatography (rp-hplc). the structural characterization and ide ... | 2014 | 24699814 |
| effective biodemulsifier components secreted by bacillus mojavensis xh-1 and analysis of the demulsification process. | the purpose of the present study was to investigate the effective components of the demulsifying bacterial strain bacillus mojavensis xh-1 and its demulsification process. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and the shotgun lc-ms/ms method were used to separate and identify proteins with efficient demulsification activity. the zeta potential changes of the emulsion before and after addition of the biodemulsifier were tested, and the relationships between oil-in-w ... | 2014 | 24275987 |
| novel angiotensin i-converting enzyme inhibitory peptides from enzymatic hydrolysates of goby (zosterisessor ophiocephalus) muscle proteins. | in recent years, food protein-derived bioactive peptides have received considerable attention because of their numerous health benefits. amongst bioactive peptides, those with antihypertensive activity are receiving special attention due to their role in cardiovascular diseases. goby protein hydrolysates (gphs) prepared by treatment with five different crude bacterial proteases were found to exhibit varying degrees of angiotensin i-converting enzyme (ace) inhibitory activity. the hydrolysate gen ... | 2013 | 23920242 |
| biofilm detection in natural unconsolidated porous media using a low-field magnetic resonance system. | the extent to which t(2) relaxation measurements can be used to determine biofouling in several natural geological sand media using a low-field (275 khz, 6.5 mt) nmr system has been demonstrated. it has been previously shown that, at high laboratory strength fields (300 mhz, 7 t), t(2) techniques can be used as a bioassay to confirm the growth of biofilm inside opaque porous media with low magnetic susceptibilities such as borosilicate or soda lime glass beads. additionally decreases in t(2) can ... | 2013 | 23256613 |
| alkaline xylanases from bacillus mojavensis a21: production and generation of xylooligosaccharides. | medium composition and culture conditions for the xylanases production by bacillus mojavensis a21 were optimized using two statistical methods: plackett-burman design applied to find the key ingredients and conditions for the best yield of enzyme production and box-behnken design used to optimize the value of the four significant variables: barley bran, nacl, agitation, and cultivation time. the optimal conditions for higher production of xylanases were barley bran 18.66 g/l, nacl 1.04 g/l, spee ... | 2012 | 22771926 |
| whole-genome sequences of bacillus subtilis and close relatives. | we sequenced four strains of bacillus subtilis and the type strains for two closely related species, bacillus vallismortis and bacillus mojavensis. we report the high-quality sanger genome sequences of b. subtilis subspecies subtilis ro-nn-1 and ausi98, b. subtilis subspecies spizizenii tu-b-10(t) and dv1-b-1, bacillus mojavensis ro-h-1(t), and bacillus vallismortis dv1-f-3(t). | 2012 | 22493193 |
| isolation and characterization of a new iturinic lipopeptide, mojavensin a produced by a marine-derived bacterium bacillus mojavensis b0621a. | three lipopeptides were isolated by bioactivity-guided fractionation from the fermentation broth of bacillus mojavensis b0621a. a new iturinic lipopeptide, named mojavensin a, was tentatively characterized by 1d, 2d nmr and ms spectroscopy, marfey's method containing a novel peptide backbone of l-asn₁, d-tyr₂, d-asn₃, l-gln₄, l-pro₅, d-asn₆, l-asn₇ and an anteiso-type of the saturated β-fatty acid side chain. compound 2 and 3 were tentatively identified as iso-c16 fengycin b and anteiso-c17 feng ... | 2012 | 22491138 |
| biological control of heterodera glycines by spore-forming plant growth-promoting rhizobacteria (pgpr) on soybean. | heterodera glycines, the soybean cyst nematode, is the most economically important plant-parasitic nematode on soybean production in the u.s. the objectives of this study were to evaluate the potential of plant growth-promoting rhizobacteria (pgpr) strains for mortality of h. glycines j2 in vitro and for reducing nematode population density on soybean in greenhouse, microplot, and field trials. the major group causing mortality to h. glycines in vitro was the genus bacillus that consisted of 92. ... | 2017 | 28704498 |
| recombination and migration rates in natural populations of bacillus subtilis and bacillus mojavensis. | we have investigated the rates of recombination and migration in native populations of two closely related, naturally competent bacillus species. native soil isolates of bacillus subtilis and bacillus mojavensis were obtained from three continents and, within north america, from populations at a range of geographical distances from one another. the rate of recombination within populations of each species was estimated from restriction-site data for three genes. recombination was shown to occur w ... | 1995 | 28568514 |
| the synergistic use of plant and isolated bacteria to clean up polycyclic aromatic hydrocarbons from contaminated soil. | biological methods of polycyclic aromatic hydrocarbons (pah) contamination elimination typically involve the transformation of contaminants to non-toxic materials by microorganisms and plants and appear to be the most effective methods available. | 2017 | 28638622 |