application of fluorescent antibody technique to determine infectivity rates of amblyomma americanium (l.) (acarina: ixodidae) salivary glands and oral secretions by theileria cervi (piroplasmorida: theileriidae).salivary glands and oral secretion from individual field collected ticks, or ticks infected with theileria cervi in the laboratory, were subjected to fluorescent antibody techniques to determine the reliability of the technique in the identification of t. cervi in either the oral secretion or salivary glands. results indicated that the fluorescent antibody technique can be used with a reasonable degree of success in the identification of infected salivary glands or oral secretion. oral secretion ...1976794455
monthly incidence of theileria cervi and seroconversion to babesia odocoilei in white-tailed deer (odocoileus virginianus) in texas.monthly monitoring of fawns collected from an area in texas endemic for theileria cervi and babesia odocoilei showed that transmission of t. cervi occurred during july and august, a time period consistent with the occurrence of amblyomma americanum. seroconversion to b. odocoilei occurred during october to december and possibly continued through january and february. the time of seroconversion was more suggestive of transmission of b. odocoilei by ixodes scapularis than by amblyomma americanum.19921512881
activity of buparvaquone against theileria cervi in white-tailed deer.buparvaquone, a naphthoquinone with known efficacy against theileria parva parva in cattle, was tested for activity against theileria cervi piroplasms in both an in vitro culture system and in vivo in experimentally infected white-tailed deer. the in vitro data showed a significant decrease in the incorporation of 3h-hypoxanthine by infected red blood cells treated with buparvaquone when compared to that seen with imidocarb and chloroquine treatment. in both intact and splenectomized deer treate ...19911902608
failure to detect infection in fallow deer (cervus dama) exposed to theileria cervi from white-tailed deer.a frozen stabilate was produced from theileria cervi sporozoites in salivary glands of adult amblyomma americanum. the stabilate was inoculated into three fallow deer (cervus dama) and two white-tailed deer (odocoileus virginianus). following inoculation, the white-tailed deer developed parasitemias as determined by blood smear examination at 11 and 13 days postexposure. repeat examination of blood from the three fallow deer for 30 days postexposure failed to reveal observable piro-plasms. these ...19873119873
the ultrastructure of sporogony in theileria cervi (bettencourt et al., 1907) in salivary glands of female amblyomma americanum (l.) ticks.the process of sporogony of theileria cervi in salivary glands of unfed and feeding amblyomma americanum females that were infected as nymphs was studied by light and electron microscopy (sem and tem). sporogony was observed only in granular acini type iii and in cells classified as complex granular. infected acini in salivary glands of both unfed and feeding ticks were enlarged. some stages of parasite development were evident with light microscopy and sem, but the specific events of sporogony ...19873125313
susceptibility of amblyomma americanum to natural and experimental infections with theileria hundred fifty amblyomma americanum were examined between march and september 1986 from cookson hills wildlife refuge in eastern oklahoma (usa). of these ticks, 11% (17 of 150) were infected with theileria cervi. field-collected nymphal ticks had an 8% (3 of 37) prevalence of infection averaging 1.0 infected acini/nymph. female ticks had a 16% prevalence of infection averaging 1.6 infected acini/female; t. cervi was not observed in salivary glands of field-collected male ticks. when laborator ...19883193564
two theileria cervi ssu rrna gene sequence types found in isolates from white-tailed deer and elk in north america.two theileria cervi ssu rrna gene sequence types, f and g, from white-tailed deer (odocoileus virginianus) and elk (cervus elaphus canadensis) isolates in north america were confirmed. previously, nucleotide sequencing through a single variable (v4) region showed the presence of ssu rrna gene types f and g in t. cervi isolates from white-tailed deer and an elk. in this study, both sequence types were found in four t. cervi isolates (two from deer and two from elk). microheterogeneity only appear ...199910479079
serologic tests for theileria cervi in white-tailed deer and for other species of theileria in cattle and sheep. 196313976630
phylogenetic analysis of theileria sp. from sika deer, cervus nippon, in japan.the 18s rrna genes of theileria species detected in sika deer, cervus nippon centralis in yamaguchi and cervus nippon yesoensis in hokkaido, were analyzed. the percent identities of the nucleotide sequences of theileria from cervus nippon centralis and cervus nippon yesoensis were more than 99%. the percent identities of the theileria sp. from sika deer and theileria sergenti, theileria buffeli and theileria cervi were 97, 96 and 95%, respectively. phylogenetic analysis of the gene sequences als ...200415063944
theileriosis in a white-tailed deer (odocoileus virginianus) fawn.a white-tailed deer (odocoileus virginianus) fawn was collected in missouri (usa) and submitted for diagnostic evaluation. necropsy and histologic examination revealed severe amblyomma americanum infestation, pronounced icterus, and marked hemosiderin deposition in the liver and spleen. whole blood evaluation revealed a normocytic normochromic anemia and a piroplasm parasitemia of approximately 70%. the piroplasm was identified as theileria cervi by polymerase chain reaction and sequencing of th ...200516456174
role of the lone star tick, amblyomma americanum (l.), in human and animal diseases.we reviewed scientific literature pertaining to known and putative disease agents associated with the lone star tick, amblyomma americanum. reports in the literature concerning the role of the lone star tick in the transmission of pathogens of human and animal diseases have sometimes been unclear and even contradictory. this overview has indicated that a. americanum is involved in the ecology of several disease agents of humans and other animals, and the role of this tick as a vector of these di ...200919054615
molecular and serologic evidence for babesia bovis-like parasites in white-tailed deer (odocoileus virginianus) in south texas.the current study was undertaken to determine if white-tailed deer in south texas harbor babesia bovis, a causative agent of bovine babesiosis. blood samples from free-ranging white-tailed deer (odocoileus virginianus) on two ranches in lasalle and webb counties were screened for b. bovis and other hemoparasites by the polymerase chain reaction (pcr) to detect the piroplasm 18s rdna. serology was conducted on selected samples to detect antibody activity to b. bovis by the immunofluorescent antib ...201020605333
detection of theileria and babesia in brown brocket deer (mazama gouazoubira) and marsh deer (blastocerus dichotomus) in the state of minas gerais, brazil.intraerythrocytic protozoan species of the genera theileria and babesia are known to infect both wild and domestic animals, and both are transmitted by hard-ticks of the family ixodidae. the prevalences of hemoprotozoa and ectoparasites in 15 free-living mazama gouazoubira, two captive m. gouazoubira and four captive blastocerus dichotomus from the state of minas gerais, brazil, have been determined through the examination of blood smears and the use of nested polymerase chain reaction (npcr). t ...201021354704
DETECTION AND IDENTIFICATION OF THEILERIA INFECTION IN SIKA DEER (CERVUS NIPPON) IN CHINA.Abstract The sika deer (Cervus nippon) is a first-grade state protected animal in China and designated a threatened species by the World Conservation Union. To detect hemoparasite infection of sika deer, blood samples were collected from 24 animals in Hubei province Deer Center. Genomic DNA was extracted and the V4 hypervariable region encoding 18S rRNA was analyzed by reverse line blot hybridization assay. PCR products hybridized with Babesia/Theileria genus-specific probes, but failed to hyb ...201122128777
DETECTION AND IDENTIFICATION OF THEILERIA INFECTION IN SIKA DEER (CERVUS NIPPON) IN CHINA.Abstract The sika deer (Cervus nippon) is a first-grade state protected animal in China and designated a threatened species by the World Conservation Union. To detect hemoparasite infection of sika deer, blood samples were collected from 24 animals in Hubei province Deer Center. Genomic DNA was extracted and the V4 hypervariable region encoding 18S rRNA was analyzed by reverse line blot hybridization assay. PCR products hybridized with Babesia/Theileria genus-specific probes, but failed to hyb ...201122176533
molecular detection and identification of piroplasms in sika deer (cervus nippon) from jilin province, china.piroplasmosis is an important disease of domestic animals and wildlife and is caused by organisms from the genera theileria and babesia. wildlife such as sika deer play an important role as reservoir hosts for several species of theileria and babesia. using blood samples collected from sika deer, we investigated the epidemiology of theileria spp. and babesia spp. in sika deer from jilin province in china and identified those species that cause pathogenic infections in sika deer.201626984286
environmental investigation following the first human case of babesiosis in tennessee.babesiosis is an emerging tick-borne zoonotic disease in the united states caused by babesia parasites. in 2009, the first case of babesiosis was documented in tennessee. environmental investigation at the reported site of tick exposure included collection of ticks and specimens from eastern cottontail rabbits (sylvilagus floridanus) and white-tailed deer ( odocoileus virginianus ) that were tested for piroplasms by molecular and serologic methods. one hundred and sixty-six ixodes scapularis tic ...201423971411
hepatozoon and theileria species detected in ticks collected from mammals and snakes in thailand.we report the detection of hepatozoon and theileria in 103 ticks from mammals and snakes in thailand. by using a genus-specific 18s rrna pcr, hepatozoon and theileria spp. were detected in 8% and 18%, respectively, of ticks (n=79) removed from mammals. of the ticks removed from snakes (n=24), 96% were infected with hepatozoon spp., but none were infected with theileria. phylogenetic analysis revealed that hepatozoon spp. detected from dermacentor astrosignatus and dermacentor auratus ticks from ...201525736475
phylogenetic analysis of bovine theileria spp. isolated in south india.the objective of the present study is to determine the phylogenetic position of the theileria organisms in blood of cattle of southern india using molecular tools. theileria annulata (namakkal isolate, tamil nadu) and three theileria field isolates (free of t. annulata) from wayanad, kerala (wayanad 1, 2, 3) were used. the small subunit ribosomal rna (ssu rrna) and major piroplasm surface protein (mpsp) gene products were cloned, sequenced and the phylogenetic tree constructed. ssu rrna gene of ...201323959494
merogonic stages of theileria cervi in mule deer (odocoileus hemionus).in february 2012, 12 farmed mule deer (odocoileus hemionus) were moved from a facility in southwestern oklahoma to a facility in southeastern oklahoma that housed 100 farmed white-tailed deer (odocoileus virginianus). between the third and fifth weeks, 9 of the 12 mule deer had died, 4 of which were submitted for necropsy. the deer were heavily infested with amblyomma americanum (lone star ticks). hematologic data from 1 deer revealed severe anemia, leukocytosis, and intraerythrocytic hemoparasi ...201324029405
detection and identification of theileria infection in sika deer ( cervus nippon ) in china.the sika deer ( cervus nippon ) is a first-grade state-protected animal in china and designated a threatened species by the world conservation union. to detect hemoparasite infection of sika deer, blood samples were collected from 24 animals in the hubei province deer center. genomic dna was extracted, and the v4 hypervariable region encoding 18s rrna was analyzed by reverse line blot hybridization assay. pcr products hybridized with babesia / theileria genus-specific probes but failed to hybrid ...201222746393
novel piroplasmid and hepatozoon organisms infecting the wildlife of two regions of the brazilian amazon.during 2009-2012, wild animals were sampled in two areas within the amazon biome of brazil, in the states of mato grosso and pará. animal tissues and blood were molecularly tested for the presence of piroplasmida (genera babesia, theileria, cytauxzoon) or hepatozoon dna. overall, 181 wild animals comprising 36 different species (2 reptiles, 5 birds, and 29 mammals) were sampled. the following piroplasmida agents were detected: cytauxzoon felis in one ocelot (leopardus pardalis), theileria cervi ...201728603688
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