| analysis of the newly identified neutralization epitopes on vp7 of human rotavirus serotype 1. | neutralizing monoclonal antibodies (mabs) directed to the vp7 protein and neutralization-resistant mutants were used to analyse the antigenic structure of vp7 of human rotavirus serotype 1. cross-neutralization tests using the mabs and the resistant mutants indicated the existence of two functionally independent neutralization epitope regions (s1 and s2) on vp7. region s1 corresponds to a single epitope domain of vp7 which has been detected previously. two mabs prepared in this study recognized ... | 1991 | 1703558 |
| clinical studies of a quadrivalent rotavirus vaccine in venezuelan infants. | phase i studies of an oral quadrivalent rotavirus vaccine were conducted in 130 venezuelan infants 10 to 20 weeks of age. the vaccine consists of a mixture of equal amounts of rhesus rotavirus (rrv) vaccine (serotype 3 [vp7]) and each of three human rotavirus-rrv reassortant strains: d x rrv (serotype 1 [vp7]), ds1 x rrv (serotype 2 [vp7]), and st3 x rrv (serotype 4 [vp7]). three different doses of the quadrivalent vaccine (0.25 x 10(4), 0.5 x 10(4), and 10(4) pfu of each component) were evaluat ... | 1990 | 2157737 |
| prospects for development of a rotavirus vaccine against rotavirus diarrhea in infants and young children. | major advances have been made in elucidating the etiologic agents of severe infantile diarrhea, and it is clear that rotaviruses are the single most important etiologic agents. progress in the development of rotavirus vaccine candidates has also moved swiftly with the "jennerian" approach, in which a related live, attenuated rotavirus strain from a nonhuman host is used as the immunizing antigen. if this strategy is not effective against all rotavirus serotypes, reassortant rotaviruses hold grea ... | 1989 | 2548276 |
| identification of an outer capsid glycoprotein of human rotavirus by concanavalin a. | an outer capsid glycoprotein of human rotavirus (serotype 1) was localized and characterized by use of lectins, electron microscopy and a modified western blotting technique. lectins with specificity for fucose, galactose, glucose and mannose were mixed with purified single- or double-shelled human rotavirus. aggregates observed by electron microscopy were obtained with double-shelled virus and concanavalin a, the only tested lectin with mannose specificity. by sds-polyacrylamide gel electrophor ... | 1984 | 6096494 |
| a monotype of human rotavirus serotype 1 involved in a diarrhea outbreak in a pediatric ward. | | 1994 | 7514161 |
| genetic variation in vp7 gene of human rotavirus serotype 1 (g1 type) isolated in japan and china. | sequence analysis of the gene encoding the major neutralization glycoprotein (vp7) was performed on 12 human isolates of serotype 1 of rotavirus in japan and china. they were examined for genetic variations among serotype 1 isolates. comparative studies of their nucleotide and deduced amino acid sequences between the 12 isolates and the wa strain revealed an overall homology of more than 92 and 96%, respectively. higher degrees of homologies were observed between wa and 2 strains (k1 and k2) in ... | 1993 | 8249305 |
| homotypic immune response to primary infection with rotavirus serotype g1. | some aspects of rotavirus humoral immunity were assessed on the basis of distinguishing serotype-specific specificities (vp4/vp7) by using rotavirus reassortants, human and animal strains in neutralization assays in serum samples obtained during the acute phase, and 1, 6 and 12 months after primary natural infection. in this study, all the infecting virus strains were characterized as g type and some also as p type. primary natural infection induces a significantly greater homotypic neutralizati ... | 1995 | 8636710 |
| the gnotobiotic piglet as a model for studies of disease pathogenesis and immunity to human rotaviruses. | gnotobiotic piglets serve as a useful animal model for studies of human rotavirus infections, including disease pathogenesis and immunity. an advantage of piglets over laboratory animal models is their prolonged susceptibility to human rotavirus-induced disease, permitting cross-protection studies and an analysis of active immunity. major advances in rotavirus research resulting from gnotobiotic piglet studies include: 1) the adaptation of the first human rotavirus to cell culture after passage ... | 1996 | 9015112 |
| viral diarrhea in children in beijing, china. | a study was undertaken from november 1994 to august 1996 to determine the role of viruses in children (< or =5 years of age) hospitalized at beijing children hospital, beijing china, for acute diarrhea. stool samples from diarrheal patients were investigated by elisa, electron microscopy, and rt-pcr for the presence of rotavirus, calicivirus, astrovirus, and adenovirus. group a rotavirus was detected in 55.9% of all diarrheal patients and comprised 82.5% of all viruses detected. group a rotaviru ... | 1999 | 10089052 |
| genotyping of rotaviruses in environmental water and stool samples in southern switzerland by nucleotide sequence analysis of 189 base pairs at the 5' end of the vp7 gene. | stool specimens from children (<4 years old) with diarrhea were collected over a 1-year period in ticino (southern region of switzerland). during the same period, environmental samples were collected from surface waters in the proximity of major water treatment plants. from treatment plants, samples were collected from the raw sewage and before the release of the treated water. from rivers, samples were collected before and after receiving the treated waters. a single-step reverse transcription ... | 2000 | 11015383 |
| safety and immunogenicity of live attenuated quadrivalent human-bovine (uk) reassortant rotavirus vaccine administered with childhood vaccines to infants. | the safety and immunogenicity of an orally administered, live rotavirus vaccine comprised of four strains, each with a titer of 10(5.3) or 10(5.8) pfu, and each having 10 genes from the uk bovine strain and the vp7 gene from human rotavirus serotype 1, 2, 3, or 4, were evaluated in adults, young children and infants in randomized, double-blind phase 1 trials. three doses of rotavirus vaccine or placebo given with childhood immunizations to infants at 2, 4, and 6 months of age were well tolerated ... | 2001 | 11535316 |
| [sequence analysis of vp7 gene from rotavirus field strain from guangzhou, china]. | to compare the difference of vp7 fragment at the nucleotide level between rotavirus guangzhou field strain r97-196 and rotavirus beijing field strain or prototype strains. | 2003 | 12870002 |
| infant immune response to human rotavirus serotype g1 vaccine candidate reassortant wi79-9: different dose response patterns to virus surface proteins vp7 and vp4. | rotavirus is the leading cause of morbidity from gastroenteritis in the developed world and the leading cause of mortality from viral gastroenteritis (estimated 600000 deaths) worldwide. g1 is the most prevalent human serotype. reassortant rotavirus between simian rotavirus rrv or bovine rotavirus wc3 and human strain rotaviruses have been extensively tested as candidate vaccines. rotavirus (rv) reassortant strain wi79-9 consists of a human (strain wi79) g1 serotype vp7 surface protein on a bovi ... | 2004 | 15014293 |
| detection and genetic characterization of group a rotavirus strains circulating among children with acute gastroenteritis in japan. | a total of 603 fecal specimens collected from july 2005 to june 2006 from children with acute gastroenteritis, encompassing five different localities in japan, were screened for group a rotavirus by reverse transcription-pcr. it was found that 117 fecal specimens (19.4%) were positive for group a rotavirus. rotavirus infection was detected continuously from november to june, with the highest prevalence in april. the g (vp7 genotypes) and p (vp4 genotypes) types were further investigated. the pre ... | 2007 | 17301134 |
| sequence analysis of the vp7 gene of human rotavirus g1 isolated in japan, china, thailand, and vietnam in the context of changing distribution of rotavirus g-types. | over the last decade, rotavirus g1 has represented the most common genotype worldwide. since 2000, the prevalence of rotavirus g1 has decreased in some countries such as japan and china. to monitor the trend of the vp7 encoding gene of rotavirus g1, we performed a sequence analysis of 74 g1 rotavirus strains isolated in japan, china, thailand, and vietnam during the period from 2002 to 2005. the phylogenetic tree showed that all of the studied g1 strains from the four countries clustered into li ... | 2007 | 17516525 |
| molecular characterization of rotaviruses, noroviruses, sapovirus, and adenoviruses in patients with acute gastroenteritis in thailand. | outbreaks of viral gastroenteritis occur worldwide including thailand. unfortunately, there is limited information since etiologic agents have not been identified in several outbreaks of nonbacterial gastroenteritis. the genotype of enteric viruses causing acute gastroenteritis in thailand was determined using reverse transcription-multiplex polymerase chain reaction and dna sequencing. from january 2006 to february 2007, stool samples were collected from patients with acute gastroenteritis of a ... | 2009 | 19107961 |
| human rotavirus vaccine is highly efficacious when coadministered with routine expanded program of immunization vaccines including oral poliovirus vaccine in latin america. | the efficacy of a rotavirus vaccine against severe rotavirus gastroenteritis when coadministered with routine expanded program on immunization (epi) vaccines including oral polio vaccine (opv) was evaluated in this study. | 2011 | 21378594 |
| development of a new cell culture-based method and optimized protocol for the detection of enteric viruses. | the development of rapid and effective methods to detect water- and food-borne enteric viruses is important for the prevention and control of mass infection. this study represents an attempt to develop a reliable cell culture-based detection system and optimize an effective and rapid protocol for the assaying of environmental samples for the presence of infectious enteric viruses. six enteric viruses were used in this study: poliovirus, coxsackie virus a9, coxsackie virus b5, human rotavirus g1, ... | 2013 | 23578702 |
| genetic diversity of rotavirus strains circulating in environmental water and bivalve shellfish in thailand. | rotavirus is a common cause of acute diarrhea in young children worldwide. this study investigated the prevalence and molecular characterization of rotavirus in environmental water and oyster samples in thailand. a total of 114 water samples and 110 oyster samples were collected and tested for group a rotavirus using rt-nested pcr. rotavirus genotype was identified by phylogenetic analysis of the vp7 genetic sequences. group a rotavirus was detected in 21 water samples (18.4%) and six oyster sam ... | 2014 | 24469269 |
| molecular analysis of vp7 gene of rotavirus g1 strains isolated from north india. | rotavirus g1 strains are the predominant cause of diarrhoea in children. universally common rotavirus vaccines (rotarix and rotateq) include g1 as the immunological component. india has recently introduced rotavirus vaccine in universal immunization programme. therefore, in the present study, vp7 gene of rotavirus g1 strains circulating in himachal pradesh, india is analysed to study their phylogenetic characteristics, and further comparative analysis was performed for assessment of their diverg ... | 2016 | 27565145 |
| predominance of rotavirus g1[p8] genotype among under-five children with gastroenteritis in mwanza, tanzania. | we analyzed stool samples from underfives with gastroenteritis for rotavirus infection between january 2010 and june 2011. a total of 393 stool specimens were examined for rotavirus infection using enzyme-linked immunosorbent assay (elisa). hundred selected positive specimens were genotyped using multiplex polymerase chain reaction. out of 393 underfives, 194 (49.4%) had rotavirus infection, with 96.9% of infected underfives being <2 years. underfives infected with rotavirus had prolonged hospit ... | 2014 | 24859323 |
| genetic and antigenic evolution profiles of g1 rotaviruses in córdoba, argentina, during a 27-year period (1980-2006). | rotavirus g1 strains represent the most common genotype that causes diarrhea in humans and has been incorporated into both, monovalent and multivalent, rotavirus licensed vaccines. the aim of this study was to determine the evolution profile of g1 rotaviruses in córdoba, argentina, over a 27-year period (1980-2006). intragenotype diversity, represented by lineages within rotavirus circulating strains, was observed. phylogenetic analysis of the vp7-gene of g1 rotavirus clinical strains showed the ... | 2013 | 23161398 |