| the sea bream liver contains receptors for growth hormone and prolactin. | different tissues of the black sea bream mylio macrocephalus including the liver, gills, intestine, muscle, gonad, swim bladder, spleen, heart and kidney were examined for the presence of prolactin and growth hormone receptors. membranes were prepared from the tissues and 125i-labeled ovine prolactin and bovine growth hormone were used as ligands. it was found that the liver contained the highest level of specific 125i-labeled ovine prolactin and bovine growth hormone binding, suggesting the exi ... | 1992 | 1417925 |
| optimization of the solubilization and renaturation of fish growth hormone produced by escherichia coli. | growth hormone (gh) enhances the growth rate of aquacultured fish and shellfish, but it is difficult to extract native gh from fish pituitary glands. however, fish recombinant gh (rgh) can be efficiently synthesized by escherichia coli cells, although it exists in denatured form in inclusion bodies (ib). we studied the solubilization of ib and the renaturation of rgh to help facilitate the production of a large amount of biologically active rgh. a 100-ml sample of rgh-producing e. coli produced ... | 1997 | 9274049 |
| effects of extracellular products of vibrio vulnificus on acanthopagrus schlegeli serum components in vitro and in vivo. | a vibrio strain ls001, originally isolated from a body surface lesion of a moribund black porgy (acanthopagrus schlegeli) in 1994 in taiwan, was identified as vibrio vulnificus. the extracellular products (ecp) of the strain were lethal to the fish, and its effects on fish serum in vitro and in vivo are described in the present study. nine major precipitation arcs were visualized in normal fish serum in a crossed immunoelectrophoresis (cie) gel using rabbit antiserum to the fish normal serum and ... | 1997 | 9670552 |
| molecular cloning, characterization, and expression of a cdna coding copper/zinc superoxide dismutase from black porgy. | a full-length complementary dna (cdna) clone encoding a putative copper/zinc superoxide dismutase (cu/zn-sod) was amplified by a polymerase chain reaction (pcr) based technique from cdna synthesized from black porgy, acanthopagrus schlegeli, mrna. nucleotide sequence analysis of this cdna clone revealed that it comprised a complete open reading frame coding for 154 amino acid residues. the deduced amino acid sequence showed slightly higher identity (72.8-78.1%) with shark and swordfish cu/zn-sod ... | 2000 | 10995377 |
| first purification of the antiquitin protein and demonstration of its enzymatic activity. | antiquitin is an evolutionarily conserved protein believed to play a role in the regulation of cellular turgor. based on sequence analysis, this protein is classified as a member of the aldehyde dehydrogenase superfamily. all previous studies on antiquitin have been confined to the nucleotide level, and the protein has never been purified and characterized. in the present investigation, the antiquitin protein was purified for the first time. an acetaldehyde-oxidizing protein was isolated from th ... | 2002 | 11959129 |
| pathogenesis of gastroenteritis caused by vibrio carchariae in cultured marine fish. | serious mortality among the cultured grouper epinephelus coioides, characterized by a swollen intestine containing yellow fluid (gastroenteritis), occurred in 1993 in taiwan. a bacterium isolated from the intestinal fluid and head kidney of moribund groupers was identified as vibrio carchariae. since then, the same vibrio species has also been isolated from moribund black sea bream acanthopagrus schlegeli, yellowfin sea bream a. latus, japanese sea bass lateolabrax japonicus, and red drum sciaen ... | 2002 | 14961260 |
| genomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (acanthopagrus schlegelii b). | hepcidin is an antimicrobial peptide and putative iron regulatory hormone previously described in mice and humans. dozens of fish hepcidins have been isolated and characterized so far. here we present seven hepcidin-like cdna sequences named as-hepc1-7, amplified from the normal commercially cultured fish (black porgy) by race-pcr. sequence analysis reveals that these seven potential hepcidin peptides have highly conserved sequences with other known hepcidins, but they are different from each ot ... | 2007 | 17574440 |
| molecular characterization and expression of three gnrh forms mrna during gonad sex-change process, and effect of gnrha on gth subunits mrna in the protandrous black porgy (acanthopagrus schlegeli). | gonadotropin-releasing hormone (gnrh) plays a pivotal role in control of reproduction and gonadal maturation in teleost fish. to investigate the action gnrh in black porgy (acanthopagrus schlegeli), we examined the mrna expression of gth subunits (gthalpha, fshbeta, and lhbeta) in the pituitary as well as plasma estradiol-17beta (e(2)) level following treatment with a gnrh analog (gnrha) in immature fish. the expression levels of gth subunits mrna and plasma e(2) level were increased after gnrha ... | 2008 | 18713632 |
| functional characterization of the elr motif in piscine elr+cxc-like chemokine. | to elucidate the functional role of piscine incomplete elr motif, the recombinant cxc and its mutants (melr and mloop) were produced in escherichia coli m15 based on the predicted mature peptide coding sequence of the black sea bream cxc (bs cxc) chemokine. assays showed that the bs rcxc proteins displayed strong ability to induce fish blood neutrophils and head kidney (hk) macrophage migration in a dose-independent manner (10 to 200 ng), both in black sea bream and common carp. although the elr ... | 2009 | 19048342 |
| profiles of antioxidant gene expression and physiological changes by thermal and hypoosmotic stresses in black porgy (acanthopagrus schlegeli). | we determined oxidative stress by measuring the expression and activity of 3 antioxidant enzymes [cu/zn-superoxide dismutase (cu/zn-sod), catalase (cat) and glutathione peroxidase (gpx)] in black porgy exposed to thermal (20 degrees c-->30 degrees c) and hypoosmotic (35 psu-->10 psu and 0 psu) stresses. the expression and activity of antioxidant enzymes were significantly higher after exposure to 30 degrees c, 10 psu, and 0psu. furthermore, we measured h(2)o(2) and lipid peroxidation (lpo) level ... | 2010 | 20172041 |
| the expression of amh and amhr2 is associated with the development of gonadal tissue and sex change in the protandrous black porgy, acanthopagrus schlegeli. | the protandrous black porgy, acanthopagrus schlegeli, has a striking life cycle, with sex differentiation at the juvenile stage, mono-male development, a bisexual gonad during the first 2 yr of life, and a male-to-female sex change (with vitellogenic oocytes) at 3 yr of age. in the present study, we investigated the possible roles of amh and amhr2 in gonadal development in a nonmammalian model organism (protandrous black porgy), especially in relation to sex differentiation, testicular and ovari ... | 2010 | 20505169 |
| occurrence of polybrominated diphenyl ethers in fish and shellfish downstream from electronic-waste recycling plants. | we measured 39 polybrominated diphenyl ethers (pbdes) in the muscle tissue of three species of fish (sciaenops ocellatus, sparus macrocephalus, and lateolabraxjaponicus) and four species of shellfish (tegillarca granosa, cyclina sinensis, sinonovacula constricta, and ostrea cucullata) that were collected downstream of electronic-waste recycling plants in taizhou, china. a total of 24 pbde congeners (pbde24) in the samples were detected. the sigma pbde24 (total pbde) ranged from 545.4 to 1688.7 n ... | 2010 | 20608509 |
| exposure of eggs to solar uv-b leads to reduced hatching rates in two sparid fishes, red sea bream pagrus major and black sea bream acanthopagrus schlegeli. | hatching success was examined under exposure to solar ultraviolet radiation (uvr) using filters to give three different light conditions [c1: uv-b, uv-a and photosynthetically active radiation (par), c2: uv-a and par, c3: par] in red pagrus major and black acanthopagrus schlegeli sea bream. hatching rate of both species was reduced by an exposure over a 2 day period to uvr and was not significantly different between two species under the three light conditions. | 2010 | 20666911 |
| kudoa iwatai and two novel kudoa spp., k. trachuri n. sp. and k. thunni n. sp. (myxosporea: multivalvulida), from daily consumed marine fish in western japan. | infection of marine fish by certain myxosporean species of the genus kudoa results in unsightly cyst formation in the trunk muscle or post-mortem myoliquefaction, causing a great economic loss to aquaculture industries, capture fisheries, and fish dealers. in addition, consumers encountering unsightly kudoa cysts in fish fillets believe them to be unknown foreign materials acquired during processing. to identify prevalent kudoa spp. encountered in daily life by the japanese population, fresh fis ... | 2010 | 21053015 |
| isolation and functional characterization of polyunsaturated fatty acid elongase (aselovl5) gene from black seabream (acanthopagrus schlegelii). | to identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (pufas), we isolated a cdna via degenerate pcr and race-pcr from acanthopagrus schlegelii with a high similarity to the elovl5-like elongases of mammals and fishes. this gene is termed aselovl5 and encodes a 294 amino acid protein. when aselovl5 was expressed in saccharomyces cerevisiae, it conferred an ability to elongate γ-linolenic acid (18:3 n-6) to di-homo-γ-linolenic acid (20:3 n-6). in ... | 2011 | 22198347 |
| Residues of fluoroquinolones in marine aquaculture environment of the Pearl River Delta, South China. | Concentrations and distributions of selected fluoroquinolones (norfloxacin, ciprofloxacin and enrofloxacin) in water, sediments and nine kinds of fish species collected from 6 sites in two marine aquaculture regions of the Pearl River Delta, China, were analyzed by using high-performance liquid chromatography with fluorescence detector (HPLC). The results showed that the concentrations of ciprofloxacin and enrofloxacin were below the limits of quantification (LOQ) in all water samples except for ... | 2011 | 21881861 |
| biological characteristics and probiotic effect of leuconostoc lactis strain isolated from the intestine of black porgy fish. | a strain of lactic acid bacteria, leuconostoc lactis, was isolated from the intestinal tract of black porgy, sparus macrocephalus, and identified by conventional biochemical characteristics and 16s rdna gene sequence analysis. the isolated strain had the ability of bile tolerance and resistance to low ph, and survived well in the trypsinase and pepsin solution. but the highly concentrated dose of trypsinase and pepsin affect the viability of the isolated strain. the isolate was resistant to seve ... | 2013 | 24516418 |
| monogeneans (platyhelminthes) from marine fishes of tongyeong, korea. | monogeneans (platyhelminthes) mostly parasitize on fins, skin and gills of fishes. in korea, the study on monogeneans is limited, although, fishes are frequently encountered with severe infection of monogeneans. hence, some of ranched and wild fishes were collected from tongyeong marine living resources research and conservation center, southern part of korea to screen and understand the infection of monogeneans. all three fish hosts were found with the infection of monogeneans including five sp ... | 2014 | 25035585 |
| infection status of estuarine fish and oysters with intestinal fluke metacercariae in muan-gun, jeollanam-do, korea. | the source of human infection with intestinal flukes was surveyed in estuarine fishes, including the dotted gizzard shad, common sea bass, common blackish goby, redlip mullet, black sea bream, and oyster collected from muan-gun, jeollanam-do, korea during august and september 2007. collected fishes and oysters were artificially digested in pepsin-hcl solution and examined under a stereomicroscope. in 36 shads (konosirus punctatus) and 20 basses (lateolabrax japonicus) examined, heterophyopsis co ... | 2012 | 22949748 |
| amoebic gill disease outbreak in marine fish cultured in korea. | in 2015, 6.7-60% mortality was observed in black seabream ( acanthopagrus schlegelii), rock bream ( oplegnathus fasciatus), and gray mullet ( mugil cephalus) farmed in the southern coast of korea. on examination, numerous amoebae were found on the gills of these 3 fish species with detection rate of 100%. some rock bream and gray mullet were coinfected with bacteria ( pseudomonas anguilliseptica, vibrio tapetis, or vibrio anguillarum). histologic examination revealed extensive hyperplastic epith ... | 2017 | 28205457 |
| functional characterization of polyunsaturated fatty acid delta 6-desaturase and elongase genes from the black seabream (acanthopagrus schlegelii). | fatty acid delta 6-desaturase (d6des) and elongases are key enzymes in the synthesis of polyunsaturated fatty acids (pufas) including arachidonic acid (ara) and eicosapentaenoic acid (epa) from microorganisms to higher animals. to identify the genes encoding d6des and elongases for pufas, we isolated each cdna with a high similarity to the d6des and elovl5-like elongases of mammals and fishes via degenerate pcr and race-pcr from acanthopagrus schlegelii. a recombinant vector expressing asd6des w ... | 2014 | 24052399 |
| induction of apoptosis in sea bream fibroblasts by vibrio harveyi haemolysin and evidence for an anti-apoptotic role of heat shock protein 70. | in this study, we exposed black sea bream, mylio macrocephalus (basilewsky), fibroblast (bsf) and silver sea bream, sparus sarba forsskål, fibroblast (ssf) cell lines to a recombinant vibrio harveyi haemolysin (vhh) and investigated mechanisms involved in apoptosis. a decrease in mitochondrial membrane potential, followed by an increase in caspase 3 activity, occurred within 2-8 h of vhh exposure, in both cell lines; however, vhh did not alter cellular levels of reactive oxygen species. as heat ... | 2012 | 27081923 |
| potential use of chitosan nanoparticles for oral delivery of dna vaccine in black seabream acanthopagrus schlegelii bleeker to protect from vibrio parahaemolyticus. | to develop an effective and easy-to-administer vaccine against vibriosis of fish, the chitosan nanoparticles-loaded dna vaccine against vibrio parahaemolyticus was studied. a dna vaccine was constructed using the outer membrane protein k (ompk) gene of v. parahaemolyticus strain (os4) and pegfp-n2 , a eukaryotic expression vector, and the construct was named pegfp-n2 -ompk (pdna). the pdna was encapsulated in chitosan particles (chitosan/pdna). the effective diameter, mean diameter and polydispe ... | 2013 | 24093149 |
| evaluation of different commercial feeds on grow-out silver black porgy, sparidentex hasta (valenciennes), for optimum growth performance, fillet quality, and cost of production. | the objective of the study was to find a cost-effective feed from three commercial feeds, namely, arasco (saudi arabia), skretting (turkey), and biomar (greece) for commercially producing silver black porgy (sobaity bream), sparidentex hasta in kuwait. for confidentiality, these feeds were randomly given code names, diets 1, 2, and 3, which were known only to the investigating staffs. the trash fish (diet 4) was used as the control. the experiment was conducted for 28 wk with grow-out sobaity br ... | 2017 | 28053574 |
| the complete mitochondrial genome of the hybrid of pagrus major (♀) × acanthopagrus schlegelii (♂). | the complete mitochondrial sequence of the hybrid from red bream (pagrus major ♀) × black porgy (acanthopagrus schlegelii ♂) was firstly determined by using the pcr-based method. the total length of the mitogenome genes of the hybrid was 17 028 bp in accordance with red bream, and it contained 13 protein-coding genes, 22 transfer rna genes, two ribosomal rna genes and one control regions. compared with the complete mitochondrial genome of its parents, the results showed that the hybrid of p. maj ... | 2016 | 26122335 |
| hepcidin gene expression induced in the developmental stages of fish upon exposure to benzo[a]pyrene (bap). | hepcidin is known to be expressed in fish with bacterial challenge and iron overload. here we first report the hepcidin expression induced in the developmental stages from embryo to fry of red sea bream (pagarus major) and in juvenile black porgy (acanthopagrus schlegelii b.) upon continuous waterborne exposure to bap. the gene expression of cyp1a1 and igl (immunoglobulin light chain) were both measured. expression of the pagarus major hepcidin gene (pm-hepc) was increased in post hatch fry at 2 ... | 2009 | 19185344 |
| rapid determination of vitellogenin in fish plasma by anion exchange high performance liquid chromatography using postcolumn fluorescence derivatization with o-phthalaldehyde. | an analytical method involving anion exchange high performance liquid chromatographic determination of vitellogenin (vtg) in fish plasma after postcolumn fluorescence derivatization with o-phthalaldehyde (opa) was developed. the retention time of vtg was about 11 min. the reagent variables for derivatization were optimized. the fluorophore was excited at 335 nm and detected at 435 nm. a calibration curve was established ranging from 0.13 to 11.28 microg. the determination limit of vtg was found ... | 2006 | 17159322 |
| complete mitochondrial genome of black porgy acanthopagrus schlegelii (perciformes, sparidae). | black porgy, acanthopagrus schlegelii, is a marine protandrous hermaphrodite and belongs to one of the most important species commercialized for food in various areas of asia. in this study, the complete mitochondrial genome of a. schlegelii has been determined. the mitogenome was 16,649 bp in length and contained 13 protein-coding genes, 2 rrna genes, 22 trna genes and 2 non-coding regions. it shared 90.2%, 82.3%, and 82.1% mitogenome sequence with acanthopagrus latus, parargyrops edita, and pa ... | 2012 | 22591205 |
| resolution of the acanthopagrus black seabream complex based on mitochondrial and amplified fragment-length polymorphism analyses. | in this study, dna analyses were employed to verify the identity of six morphologically similar species that occur in the coastal waters of taiwan: the black seabream complex (acanthopagrus latus, acanthopagrus schlegelii, acanthopagrus sivicolus, acanthopagrus taiwanensis, acanthopagrus chinshira and acanthopagrus pacificus). amplified fragment-length polymorphism (aflp) analyses clearly distinguished the same six species that are morphologically diagnosable based on subtle differences in scale ... | 2011 | 22026601 |
| monogenea of chinese marine fishes. xiv. two new species of microcotylidae from fishes of the south china sea. | two new species of microcotylid monogeneans are described. solostamenides platyorchis n. sp. was obtained from the gills of mugil cephalus (mugilidae) and polylabroides guangdongensis n. sp. from the gills of sparus macrocephalus, s. berda and acanthopagrus latus (sparidae). solostamenides platyorchis is morphologically similar to s. mugilis (vogt, 1878) unnithan, 1971 and s. pseudomugilis (hargis, 1956) unnithan, 1971 in the structure of the copulatory organ, but differs from them in the number ... | 2001 | 11213206 |
| primary males guide the femaleness through the regulation of testicular dmrt1 and ovarian cyp19a1a in protandrous black porgy. | controlling the development of the sexes is critically important for the broodstock management in aquaculture. sex steroids are widely used for sex control of fish. however, hermaphroditic fish have a plastic sex, and a stable sex is difficult to maintain with sex steroids. we used the black porgy (acanthopagrus schlegelii) as a model to understand the possible mechanism of sexual fate decision. low exogenous estradiol (e2) induced male development. in contrast, high exogenous e2 induced the reg ... | 2017 | 28188743 |
| role of mariculture in the loading and speciation of mercury at the coast of the east china sea. | the effects of mariculture on mercury (hg) contamination and speciation in water, sediment and cultured fish in a typical mariculture zone located in xiangshan bay, zhejiang province, east china, were studied. water, sediment and fish samples were collected from mariculture sites (ms) and from corresponding reference sites (rs) 2500 m away from the ms. the thg concentration in overlying water in xiangshan bay reached as high as 16.6 ± 19.5 ng l(-1), indicating that anthropogenic sources in this ... | 2016 | 27613319 |
| isolation and characterization of eight novel microsatellite markers in acanthopagrus schlegelii. | acanthopagrus schlegelii is a warm temperate demersal fish, which inhabits the sediment substrate or rocky reefs in shallow seas. as this fish is a nutritionally endowed species with good palatability, it is a highly valuable commercial species for aquaculture and has a long historical standing in western pacific countries. because the population of this fish is currently declining in china, studies and measures aimed at addressing this decline are needed. in this study, eight microsatellite mar ... | 2016 | 27323186 |
| the testis is a primary factor that contributes to epigenetic modifications in the ovaries of the protandrous black porgy, acanthopagrus schlegelii. | in most hermaphroditic fish, the sexual phase of the gonad responds to external stimuli so that only one sex remains functional while the other sex becomes dormant. however, protandrous black porgy are male during their first two reproductive cycles. estradiol (e2)-induced female growth results in a transient and immature female, and the sexual phase reverts from female to male after e2 is withdrawn. conversely, excising the testis results in a precocious female when performed during the second ... | 2016 | 27103447 |
| activation of brain steroidogenesis and neurogenesis during the gonadal differentiation in protandrous black porgy, acanthopagrus schlegelii. | the early brain development, at the time of gonadal differentiation was investigated using a protandrous teleost, black porgy. this natural model of monosex juvenile fish avoids the potential complexity of sexual dimorphism. brain neurogenesis was evaluated by histological analyses of the diencephalon, at the time of testicular differentiation (in fish between 90 and 150 days after hatching). increases in the number of both nissl-stained total brain cells, and pcna-immunostained proliferative br ... | 2016 | 25980979 |
| morphological development of larvae and juveniles of acanthopagrus schlegeli. | newly hatched black porgy larvae (acanthopagrus schlegeli) swam to the surface, with the mouth and anus still closed and were 1.90-2.11 mm (mean, 2.0 mm) in total length (tl). the larvae were 2.71-2.94 mm tl (mean, 2.82 mm) on day 2 after hatching. at this time, about two-thirds of the yolk was absorbed, the bladder and intestines had formed, and the mouth and anus were open. total length was 4.32-4.66 mm (mean, 4.45 mm) at the post-larval stage on days 5-6 after hatching, and the yolk and oil g ... | 2014 | 25949202 |
| the potential role of amh to prevent ectopic female development in testicular tissue of the protandrous black porgy, acanthopagrus schlegelii. | in most vertebrates, hermaphroditism results in infertility. however, hermaphroditism occurs in 6% of teleosts, which primarily undergo protogyny. here, to elucidate the transient stage from gonochorism to hermaphroditism, juvenile black porgies as a model animal were fed a diet containing estradiol (e2) for 3 mo, followed by withdrawal of e2 treatment. the e2-terminated fish had ectopically located oocytes in the regenerated testes. antimüllerian hormone (amh) was strongly expressed in the sert ... | 2015 | 25855263 |
| isolation and characterization of thirteen polymorphic microsatellite loci from black porgy (acanthopagrus schlegeli). | | 2014 | 25572074 |
| bioremediation using gracilaria chouae co-cultured with sparus macrocephalus to manage the nitrogen and phosphorous balance in an imta system in xiangshan bay, china. | a cage experiment using the red alga gracilaria chouae co-cultured with the black seabream sparus macrocephalus in xiangshan bay, china was conducted to measure the nutrient flux of the integrated multi-trophic aquaculture (imta) system. results showed that trash fish were the main nutrient input contributor and adult fish were the main nutrient output contributor in the system. contents of n and p in adult fish accounted for 54.45% and 59.48% of n and p in trash fish and fry, which suggests tha ... | 2015 | 25561001 |
| feasibility of infrared and raman spectroscopies for identification of juvenile black seabream (sparus macrocephalus) intoxicated by heavy metals. | the potential application of infrared and raman spectroscopies was explored as rapid and nondestructive tools for the identification of juvenile black seabream samples intoxicated by heavy metals (zn, cu, and cd). discrimination models were established on the basis of the infrared and raman spectral data using three calibration methods, namely, partial least-squares discriminant analysis, least-squares support vector machines, and random forest. the combination of two spectroscopies was studied, ... | 2013 | 24261418 |
| conserved sex-specific timing of meiotic initiation during sex differentiation in the protandrous black porgy acanthopagrus schlegelii. | meiosis is an essential mechanism of gametogenesis for all sexually reproducing species. in vertebrates, one conserved aspect of sex differentiation is that female embryonic germ cells enter meiosis earlier than male germ cells. in some lower vertebrates, female germ cells proliferate prior to entering meiosis, whereas male cells remain in mitotic arrest. protandrous black porgy fish, acanthopagrus schlegelii, have a dramatic life cycle involving a characteristic sex change. black porgy are func ... | 2013 | 23616595 |
| neuropeptide y in black seabream acanthopagrus schlegelii: identification, distribution and mrna expression responses to ghrelin. | the coding region of neuropeptide y (npy) complementary (c)dna was cloned from the hypothalamus rna of black seabream acanthopagrus schlegelii, including 297 bp coding for prepro-npy of 98 amino acids. real-time reverse transcriptase-polymerase chain reaction was performed to determine a. schlegelii npy gene expression; npy messenger rna (mrna) was expressed highly in the brain and stomach. treatment with acylated ghrelin significantly up-regulated npy mrna level in the hypothalamus, suggesting ... | 2013 | 23557320 |
| oocytes survive in the testis by altering the soma fate from male to female in the protandrous black porgy, acanthopagrus schlegeli. | in fish, hermaphroditism is derived from gonochorism. no ancient ancestry and no single sex-determining mechanism are involved in the hermaphroditic fish. furthermore, hermaphroditic fish have a common set of transcriptional regulators that are involved in gonadal differentiation. however, the origins and evolution of hermaphroditism in fish remain far from understood. in the protandrous black porgy (acanthopagrus schlegeli bleeker), the ovotestis is separated by connective tissue, and no inters ... | 2013 | 23197163 |
| effects of tea polyphenol coating combined with ozone water washing on the storage quality of black sea bream (sparus macrocephalus). | the present research evaluated the effects of tea polyphenol (tp) combined with ozone water (o(3)) on the quality of black sea bream (sparus macrocephalus) over a period of 15 days storage at 4°c. a solution of tp (0.2%, w/v) was used to coat the fish after washing with ozone water (1mg/l). fish physicochemical (ph, k value, peroxide value, thiobarbituric acid, total volatile basic nitrogen, trimethylamine, texture, and colour), sensory, and bacteriological characteristics were all analysed. tp+ ... | 2012 | 22980890 |
| characterization of genes encoding prolactin and prolactin receptors in starry flounder platichthys stellatus and their expression upon acclimation to freshwater. | this study aims to investigate the genes encoding prolactin (prl) and prolactin receptors (prlr) and their tissue-specific expression in starry flounder platichthys stellatus. starry flounder prl gene consisting of five exons encodes an orf of 212 amino acid residue comprised of a putative signal peptide of 24 amino acids and a mature protein of 188 amino acids. it showed amino acid identities of 73 % with tuna thunnus thynnus, 71 % with black porgy acanthopagrus schlegelii, 69 % with nile tilap ... | 2013 | 22843312 |
| increase in estrogen signaling in the early brain of orange-spotted grouper epinephelus coioides: a mini-review. | despite neurosteroidogenic enzymes are playing important roles in the regulation of brain development and function, the potential link between brain and gonad by the action of steroid hormones during gonadal sex differentiation is still not clear in teleosts. in this mini-review, we summarized our understanding on the early brain development related to the synthesis of neurosteroids and receptor signaling during gonadal sex differentiation in protogynous orange-spotted grouper, epinephelus coioi ... | 2013 | 22692774 |
| three novel myxobolid species of genera henneguya and myxobolus (myxosporea: bivalvulida) from marine fish in japan. | myxosporean genera henneguya and myxobolus (bivalvulida: myxobolidae) are closely related in morphology and molecular phylogeny, speciose with approximately 1,000 nominal species. the majority of them are recorded from freshwater fish worldwide, and few are known from marine fish. in this study, three myxobolid spp. are described from marine fish around japan. two novel henneguya spp., henneguya ogawai sp. n. and henneguya yokoyamai sp. n., are described from two black sea breams (acanthopagrus ... | 2012 | 22476601 |
| the switch of secondary sex determination in protandrous black porgy, acanthopagrus schlegeli. | hermaphrodites have both sexes during their life, including an initial primary sex determination and in later stage maintenance one of the sexual fates (secondary sex determination). sex change (secondary sex determination) occurs in animals, but it is lost in amphibians through, mammals in vertebrates. teleosts have various strategies and mechanisms of sex determination including genetic and environmental cues. however, the mechanisms by which the cues guide sex change are complicated in fish. ... | 2013 | 22411079 |
| unifying prolonged copper exposure, accumulation, and toxicity from food and water in a marine fish. | the link between metal exposure and toxicity is complicated by numerous factors such as exposure route. here, we exposed a marine fish (juvenile blackhead seabream acanthopagrus schlegelii schlegelii) to copper either in a commercial fish diet or in seawater. copper concentrations in intestine/liver were correlated linearly with influx rate, but appeared to be less influenced by uptake pathway (waterborne or dietary exposure). influx rate best predicted cu accumulation in the intestine and liver ... | 2012 | 22372853 |
| testicular dmrt1 is involved in the sexual fate of the ovotestis in the protandrous black porgy. | in hermaphroditic fish, the ovotestis can respond to external stimuli so that only one type of gonadal tissue (either ovarian or testicular tissue) will remain reproductively active and the other will recede to a rudimentary stage. however, the molecular mechanism for sexual fate determination is still poorly understood in hermaphroditic fish. in the present study, we examined whether sexual fate determination with respect to testis development is due to differential expression of dmrt1. express ... | 2012 | 22034528 |
| chalimus stages of caligus latigenitalis (copepoda:caligidae) parasitic on blackhead seabream from japanese waters, with discussion of terminology used for developmental stages of caligids. | the first and third chalimus stages and chalimus adult (previously known as young adult) of caligus latigenitalis are described based on new material collected from the body surface of a heavily infected wild blackhead seabream acanthopagrus schlegelii schlegelii from hiroshima bay, japan. the second and fourth chalimus stages of the same species are redescribed. adults of c. latigenitalis are characterized by possessing 2 stout marginally indented processes at the base of 2 terminal spines at d ... | 2011 | 21506770 |
| development of the genital duct system in the protandrous black porgy, acanthopagrus schlegeli. | protandrous black porgies, acanthopagrus schlegeli, have a striking life cycle, which includes early sex differentiation, bisexual gonads, and a male-to-female sex change at three years of age. we found novel features of and insights into the development of the genital duct system in relation to the gonadal stage during early gonadal development and natural sex change. we found that the genital ducts developed at 16-20 weeks of age during sex differentiation. the gonad developed "ovarian cavity ... | 2011 | 21290611 |
| molecular characterization of hepcidin as-hepc2 and as-hepc6 in black porgy (acanthopagrus schlegelii): expression pattern responded to bacterial challenge and in vitro antimicrobial activity. | there are more diversified isoforms of the hepcidin gene that exist in fishes than in mammals, and elucidating the differences between these isoforms should provide insight into the functioning of hepcidin in fishes. in our study, as-hepc2 and as-hepc6 hepcidin isoforms from black porgy were characterized for their in vivo expression patterns following bacterial challenge, and their in vitro antimicrobial activities against gram-positive and gram-negative bacteria as well as fungi. as a result, ... | 2011 | 21073978 |
| a new species of capillaria (nematoda: capillariidae) from the intestine of the marine fish acanthopagrus schlegelii schlegelii (sparidae) from japan. | a new nematode species, capillaria acanthopagri n. sp. (capillariidae), is described from the intestine of the marine fish (black porgy) acanthopagrus schlegelii schlegelii (bleeker) from coastal waters of the western north pacific ocean off kochi, shikoku island, japan. the new species, belonging to the subgenus neocapillaria moravec, 1987 , differs from other congeneric species of this subgenus from marine fishes (with the exception of c. navonae timi, rossin and lanfranchi, 2006 ) mainly in t ... | 2010 | 20738201 |
| regulation of two forms of gonadotropin-releasing hormone receptor gene expression in the protandrous black porgy fish, acanthopagrus schlegeli. | two gnrh receptors (gnrh-r i and gnrh-r ii) were obtained in protandrous black porgy (acanthopagrus schlegeli). we investigated their tissue distribution, developmental/seasonal changes and regulation of expression using in vivo and in vitro (primary cultures of dispersed pituitary cells) approaches. the relative expressions of gnrh-rs in the pituitary and gonad were as follows: pituitary: gnrh-r i > gnrh-r ii; testicular tissue: gnrh-r i > gnrh-r ii; ovarian tissue: gnrh-r i = gnrh-r ii. gnrh-r ... | 2010 | 20398731 |
| sex differentiation and sex change in the protandrous black porgy, acanthopagrus schlegeli. | protandrous black porgy fish, acanthopagrus schlegeli, have a striking life cycle with a male sex differentiation at the juvenile stage and male-to-female sex change at 3 years of age. we had characterized the sex differentiation and sex change in this species by the integrative approaches of histology, endocrine and molecular genetics. the fish differentiated in gonad at the age around 4-months and the gonad further developed with a bisexual gonad for almost for 3 years and sex change at 3 year ... | 2010 | 19917286 |
| effect of dietary ascorbate on lipogenesis and lipolysis activities in black sea bream, acanthopagrus schlegelii. | to assess the effect of dietary ascorbate on lipid metabolism, 1-year black sea bream (acanthopagrus schlegelii) were reared on a casein-based purified diet and an ascorbate fortified diet (1,100 mg of l: -ascorbyl-2- monophosphate-mg/kg diet). the fortified ascorbate was effectively incorporated into the fish body and elevated muscle carnitine content. fortifications of dietary ascorbate depressed activities of glucose-6-phosphate dehydrogenase and nadp-isocitrate dehydrogenase as lipogenic enz ... | 2010 | 19685218 |
| copper uptake kinetics and regulation in a marine fish after waterborne copper acclimation. | the uptake kinetics and regulation of copper in a marine predatory fish, the black sea bream acanthopagrus schlegeli after acclimation to waterborne cu were examined, using radiotracer techniques. the dissolved cu uptake followed a linear pattern during the time of exposure, and the calculated uptake rate constant was 6.24 l kg(-1) day(-1). the efflux rate constant was 0.091 day(-1) following dietary uptake of cu, and the dietary assimilation efficiency (ae) of cu varied between 1.7% and 10.9% a ... | 2009 | 19683350 |
| wnt4 is associated with the development of ovarian tissue in the protandrous black porgy, acanthopagrus schlegeli. | the protandrous black porgy, acanthopagrus schlegeli, has a striking life cycle with sex differentiation at the juvenile stage, mono-male development, and male-to-female sex change (with vitellogenic oocytes) at age 3 yr. in the present study, we investigated the possible roles of wnt4 in gonadal development in a nonmammalian model organism (protandrous black porgy), especially in relation to sex differentiation, ovarian growth, and sex change. fish of various ages were treated with estradiol (e ... | 2009 | 19605791 |
| quantitative mrna expression of sox3 and dmrt1 during sex reversal, and expression profiles after gnrha administration in black porgy, acanthopagrus schlegeli. | we cloned full-length sox3 cdna from testis of black porgy, acanthopagrus schlegeli. black porgy sox3 cdna consists of 897 base pairs (bp) and encodes a protein of 298 amino acids. we have investigated the expression pattern of sox3 and dmrt1 mrna during the sex-reverse process from male to female (immature testis, mature testis, testicular portion of mostly testis, ovarian portion of mostly testis, testicular portion of mostly ovary, ovarian portion of mostly ovary and ovary). the expression of ... | 2009 | 19490951 |
| gender-related expression of tralpha and trbeta in the protandrous black porgy, acanthopagrus schlegeli, during sex change processes. | we cloned the thyroid hormone receptor alpha (tralpha) and beta (trbeta) cdnas from the ovaries of the protandrous black porgy and compared the expression levels of tralpha and trbeta mrna during the sex change in black porgy. we observed that the tralpha mrna by quantitative real-time pcr and protein levels by western blot were highest in the mature ovaries. additionally, trbeta mrna levels were only expressed highly in the mature ovaries when compared to any other gonadal stages. then, we inje ... | 2010 | 19481082 |
| adaptation of visual spectra and opsin genes in seabreams. | three species of seabreams, acanthopagrus berda, acanthopagrus schlegelii and pagrus major, living at different depths, were chosen to investigate how visual spectra and opsin genes evolve in response to various photic environments. the lambda max of photoreceptors and opsin genes were measured and cloned from these species. eight to twelve nm spectral shifts in the rod and blue cone cells were observed between the deep-sea, p. major, and shallow-sea species, a. berda and a. schlegelii. furtherm ... | 2009 | 19422842 |
| age-dependent differential expression of genes involved in steroid signalling pathway in the brain of protandrous black porgy, acanthopagrus schlegeli. | the mechanisms underlying brain sex differentiation in animals are poorly understood. in the present study, using black porgy, acanthopagrus schlegeli, as primary experimental model, we investigated the temporal expression patterns of receptors for androgen (ar) and estrogen (esr1 and esr2a) in the brain during posthatching ages and analyzed them against the timing of gonadal germ cell development. we hypothesized that endogenous estrogens naturally masculinize the brain of black porgy. the expr ... | 2009 | 19224561 |
| salinity effects on the expression of osmoregulatory genes in the euryhaline black porgy acanthopagrus schlegeli. | black porgy is a marine euryhaline species with a capacity to cope with demands in a wide range of salinities and thus is a perfect model-fish to study osmoregulatory responses to salinity-acclimated processes and their hormonal control. the present study was performed to understand the regulatory changes in hormone, hormone receptors and important osmoregulatory genes in pituitary, gill, intestine and kidney in response to acute salinity stress. transcript levels were analyzed by quantitative r ... | 2009 | 19116154 |
| molecular characterization of gonadotropin subunits and gonadotropin receptors in black porgy, acanthopagrus schlegeli: effects of estradiol-17beta on mrna expression profiles. | the cdnas of three gonadotropin (gth) subunits (gthalpha, fshbeta, and lhbeta) and two gth receptors (fshr and lhr) from pituitary and gonads of black porgy were cloned. the nucleotide sequences of the gthalpha, fshbeta, and lhbeta cdna were 354, 363, and 414 base pairs (bps) in length with open reading frames (orf) encoding peptides of 117, 120, and 137 amino acids, respectively. the fshr and lhr cdna was 2118 and 2076 bps in length with orfs encoding peptides of 705 and 691 amino acids, respec ... | 2009 | 19061966 |
| dual roles of cyp19a1a in gonadal sex differentiation and development in the protandrous black porgy, acanthopagrus schlegeli. | protandrous black porgy fish, acanthopagrus schlegeli, have a striking life cycle, with male sex differentiation at the juvenile stage, a bisexual gonad during first 2 yr of life, and a male-to-female sex change (with vitellogenic oocytes) at 3 yr of age. the present study investigated the role of aromatase (cyp19a1a/cyp19a1a) in gonadal development in this species, especially in relation to sexual differentiation and sex change. fish of various ages were treated with estradiol (e2) or aromatase ... | 2008 | 18667752 |
| cloning and expression of aquaporin 1 and arginine vasotocin receptor mrna from the black porgy, acanthopagrus schlegeli: effect of freshwater acclimation. | we cloned complementary dna (cdna) encoding aquaporin 1 (aqp1) and arginine vasotocin receptor (avt-r) from gill and kidney tissue of the black porgy (acanthopagrus schlegeli), respectively. black porgy aqp1 cdna consists of 786 base pairs (bp) and encodes a protein of 261 amino acids, and avt-r partial cdna consists of 606 bp. to investigate the osmoregulatory abilities of black porgy in different salinities (35 per thousand seawater, sw, 10 per thousand sw, freshwater, fw), we examined the exp ... | 2008 | 18649036 |
| characterization of estrogen receptor beta2 and expression of the estrogen receptor subtypes alpha, beta1, and beta2 in the protandrous black porgy (acanthopagrus schlegeli) during the sex change process. | estrogens play an important role in many physiological processes in both female and male vertebrates, mediated by specific nuclear receptor, estrogen receptors (ers). we have isolated a third er (erbeta2), which was found to contain 2004 nucleotides including an open reading frame that encodes 667 amino acids. we have also cloned eralpha and erbeta1 from the published information (genbank accession nos. ay074780 and ay074779) and investigated the expression pattern of these er subtypes in the go ... | 2008 | 18455458 |
| expression of warm temperature acclimation-related protein 65-kda (wap65) mrna, and physiological changes with increasing water temperature in black porgy, acanthopagrus schlegeli. | we isolated the warm temperature acclimation-related protein 65-kda (wap65) cdna from the liver of black porgy and investigated the expression by increasing water temperature in black porgy, acanthopagrus schlegeli. black porgy wap65 full-length cdna consists of 1,338 nucleotides, including an open reading frame, predicted to encode a protein of 425 amino acids and showed high homology to pufferfish (79%), medaka (73%), carp (70%), and goldfish (68%) wap65. increase in water temperature (20 degr ... | 2008 | 18273879 |
| influence of fasting on muscle composition and antioxidant defenses of market-size sparus macrocephalus. | the study was conducted to investigate fasting effects on flesh composition and antioxidant defenses of market-size sparus macrocephalus. two hundred fish (main initial weight 580 g) were divided into two groups (control and fasted) and reared in 6 cages. after two weeks of adaptation, group i fasted for 28 d; group ii was fed normally as a control. in 3, 7, 14, 21 and 28 d, 6 fish per group were sampled for proximate flesh composition, liver antioxidant enzyme activities and malondialdehyde fle ... | 2007 | 18257126 |
| polycyclic aromatic hydrocarbon (pah) metabolites in marine fishes as a specific biomarker to indicate pah pollution in the marine coastal environment. | in this study, analysis methods for the pah metabolites of naphthalene (na), pyrene (py) and benzo(a)pyrene (bap) with different benzo-rings (2-4-5 rings respectively) were developed and the metabolism kinetics of py and bap in marine fishes were studied. two pah metabolites of na and py, namely 1-naphthol (1-oh na) and 1-hydroxy pyrene (1-oh py), were determined using the fixed wavelength fluorescence (ff) method, and the bap metabolite, 3-hydroxy benzo(a)pyrene (3-oh bap), was determined using ... | 2008 | 18205051 |
| the expression of nr0b1 and nr5a4 during gonad development and sex change in protandrous black porgy fish, acanthopagrus schlegeli. | protandrous black porgy fish, acanthopagrus schlegeli, have a striking life cycle, with a mono-male sex differentiation at the juvenile stage and male-to-female sex change at 3 yr of age. we report for the first time integrative molecular data on these interesting phenomena. sex differentiation occurred between 4 and 5 mo of age. testicular nr5a4 transcripts increased to high levels during sex differentiation (5 mo old), whereas nr0b1 (dax-1) did not increase until the age of 8 mo. high nr5a4 an ... | 2008 | 17959853 |
| gastrointestinal uptake of cadmium and zinc by a marine teleost acanthopagrus schlegeli. | gastrointestinal metal uptake represents a potential route for metal bioaccumulation in marine fish. drinking of seawater for osmoregulation causes constant waterborne exposure of the gastrointestinal tract. tissue specific cd and zn accumulation and distribution were investigated in juvenile black sea bream (acanthopagrus schlegeli) exposed to waterborne cd (5.7 nm) and zn (2.6 nm) for 4 h-7 days. the intestine accumulated a large portion of the cd (43-58%) and zn (18-28%), and had the highest ... | 2007 | 17904660 |
| cloning and expression of na+/k+ -atpase and osmotic stress transcription factor 1 mrna in black porgy, acanthopagrus schlegeli during osmotic stress. | we cloned complementary dna (cdna) encoding the na(+)/k(+)-atpase (nka) and the osmotic stress transcription factor 1 (ostf1) from the kidney and gill, respectively, of the black porgy, acanthopagrus schlegeli. black porgy nka full-length cdna consists of 3078 base pairs (bp) and encodes a protein of 1025 amino acids; ostf1 partial cdna consists of 201 bp. to investigate the osmoregulatory ability of black porgy when black porgy were transferred to freshwater (fw), we examined the expression of ... | 2008 | 17900954 |
| molecular characterization of myostatin in black seabream, acanthopagrus schlegelii. | myostatin is a negative regulator of skeletal muscle growth and has a potential application in aquaculture. the black seabream myostatin gene was cloned and sequenced. it had three exons encoding a protein of 382 amino acids. a 90 bp 5'-untranslated region (utr) and a 536 bp 3'-utr were obtained by race. four microsatellite sequences, a (cag)9, a (tc)12, a (ca)16 repeat and an "imperfect" (ca)25 microsatellite, were found in the myostatin. two introns were 329 and 742 bp in length, respectively. ... | 2008 | 17852358 |
| effects of alpha-tocopheryl acetate supplementation in preslaughter diet on antioxidant enzyme activities and fillet quality of commercial-size sparus macrocephalus. | this study examined the effects of dietary alpha-tocopheryl acetate supplementation on antioxidant enzyme activities and fillet quality in commercial-size sparus macrocephalus. three hundred fish [main initial weight (350+/-12) g] were divided into three groups (e250, e500 and e1000) and reared in 9 cages. the fish were fed for 8 weeks with three diets containing different levels of dietary alpha-tocopheryl acetate (289, 553, 1 069 mg/kg). over the experimental period, fish were fed to satiation ... | 2007 | 17726750 |
| waterborne cadmium and zinc uptake in a euryhaline teleost acanthopagrus schlegeli acclimated to different salinities. | metal uptake and toxicity in marine fish are usually much lower than those in freshwater fish, but the underlying mechanisms remain unclear. in this study, we investigated cd and zn uptake by the euryhaline black sea bream (acanthopagrus schlegeli) over a salinity range from 0 to 35 psu. cd and zn uptake increased as salinity decreased. the gills were the most sensitive organs in response to salinity change, and played a more important role in cd and zn uptake at a lower salinity. cd and zn upta ... | 2007 | 17675173 |
| developmental expression of key steroidogenic enzymes in the brain of protandrous black porgy fish, acanthopagrus schlegeli. | in the present study, we tested the hypothesis that the brain of the black porgy fish, acanthopagrus schlegeli, has the capacity for de novo steroidogenesis and that these neurosteroids may impact sex differentiation. gonadal histology and dmrt1 gene expression revealed that the fish were not sex differentiated until 155 dah (days after hatching). we further demonstrated the developmental expressions of the mrnas encoding for four key neurosteroidogenic enzymes, namely, the cytochrome p450 side ... | 2007 | 17620106 |
| size-dependence of the potential for metal biomagnification in early life stages of marine fish. | we investigated the bioaccumulation of metals (cd, se, and zn) in different juvenile sizes of black sea bream acanthopagrus schlegeli by applying a biokinetic model. a series of experiments were conducted to determine the physiological kinetic parameters, including uptake rate constant of waterborne metals, ingestion rate, assimilation efficiency of dietary metals, efflux rate constant, and specific growth rate as a function of fish size. body concentration of metals as a function of body size w ... | 2007 | 17447565 |
| molecular cloning of pepck and stress response of black porgy (acanthopagrus schlegeli) to increased temperature in freshwater and seawater. | stress responses to increased temperature in black porgy reared in freshwater (fbp) and seawater (sbp) were examined via endocrinological and blood physiological methods. a rise in temperature increased plasma cortisol levels, which were significantly higher in fbp compared to sbp. the stimulated expression of phosphoenolpyruvate carboxykinase (pepck) mrna in liver might result from the high cortisol level, and this explains the observed higher plasma glucose levels in fbp versus sbp. full-lengt ... | 2007 | 17418846 |
| black porgy (acanthopagrus schlegeli) prolactin cdna sequence: mrna expression and blood physiological responses during freshwater acclimation. | to investigate the consequences of freshwater (fw) transfer, we studied the prolactin (prl) cdna sequence and its mrna expression, and physiological responses in the black porgy (acanthopagrus schlegeli). we cloned and characterized cdna encoding its prl from the pituitary gland. black porgy prl cdna consists of 1492 bp and encodes a protein of 212 amino acids including 24 signal peptides. reverse transcription-pcr showed the prl mrna expression in the pituitary gland. expression of pituitary gl ... | 2007 | 17329140 |
| effects of gonadotropin-releasing hormone analog (gnrha) on steroidogenic factor-1 (sf-1) and estrogen receptor beta (erbeta) gene expression in the black porgy (acanthopagrus schlegeli). | we examined effects of gnrha on expression of steroidogenic factor-1 (sf-1) and estrogen receptor beta (erbeta) in the pituitary and gonad of protandrous black porgy (acanthopagrus schlegeli). fish were intraperitoneally injected with 0.2 microg gnrha/g fish and then pituitary, gonad and plasma were sampled at 0, 6, 12, 24 and 48 h after injection. in gonad, the mrna levels of the sf-1 were high at 6 h post injection, and then continuously decreased until 24 h; high expression of erbeta mrna lev ... | 2007 | 17276717 |
| impact of heavy metals and organochlorines on hsp70 and hsc70 gene expression in black sea bream fibroblasts. | the aim of the present study was to investigate the effects of environmentally important heavy metals and organochlorines on the transcriptional profiles of genes coding for heat shock cognate 70 (hsc70) and inducible heat shock protein 70 (hsp70) in a black sea bream fibroblast cell line. using the nucleotide sequence information, from the cloned genes, specific reverse transcriptase-polymerase chain reaction (rt-pcr) methods were devised to test the effects of heavy metals (cd2+, cu2+ and ni2+ ... | 2006 | 16780970 |
| alteration of dissolved cadmium and zinc uptake kinetics by metal pre-exposure in the black sea bream (acanthopagrus schlegeli). | we first examined the uptake kinetics of cd and zn in the juvenile marine black sea bream (acanthopagrus schlegeli) over a wide range of ambient cd and zn concentrations, and the relationships with metal accumulation (uptake rate and amount of nonexchangeable surface binding) were established for different fish tissues. both cd and zn accumulation in the body increased linearly with exposure time after the initial metal surface binding. the dissolved cd and zn uptake rate constants were 2.64 and ... | 2006 | 16704063 |
| hormones and reproduction in scleractinian corals. | most broadcast spawning scleractinian corals synchronously release gametes during a brief annual spawning period. in southern taiwan, the mass spawning of scleractinians occurs in lunar mid-march. the exact cues triggering this annual phenomenon remain unclear. a scleractinian coral, euphyllia ancora has been selected as a model for the hormones and reproduction studies. testosterone (t) and estradiol (e2) in free and glucuronided forms were identified and consistently detected in coral polyps t ... | 2006 | 16488637 |
| the presence and ancestral role of gonadotropin-releasing hormone in the reproduction of scleractinian coral, euphyllia ancora. | the objectives of this study were to investigate the presence of immunoreactive gnrh (irgnrh) in scleractinian coral, euphyllia ancora, study its seasonal variation, and evaluate its biological activity. irgnrh was detected and quantified in coral polyps. the biological activity of coral irgnrh was tested on pituitary cells from black porgy by evaluating its ability to stimulate lh release. coral extracts (10(-9)-10(-5) m irgnrh) as well as mammalian (m) gnrh agonist (10(-10)-10(-6) m) had a sim ... | 2006 | 16195400 |
| differential expression of neural and gonadal aromatase enzymatic activities in relation to gonadal development in japanese eel, anguilla japonica. | the objectives of the present study were to investigate the enzymatic characteristics of brain aromatase in japanese eel, anguilla japonica, as well as the correlations between aromatase activities in various tissues (brain regions, pituitary, and gonads) and ovarian development. eel brain aromatase exhibited a k(m) of 75 nm and a v(max) of 1.14 fmol/min mg protein (91.5 fmol/h mg protein), indicating an enzymatic activity much lower than in other teleosts but similar to that in mammals. this su ... | 2005 | 16106412 |
| effects of zn pre-exposure on cd and zn bioaccumulation and metallothionein levels in two species of marine fish. | zinc is an essential trace metal but also a potential toxicant to aquatic organisms. in this study, two juvenile marine fish species, the black sea bream acanthopagrus schlegeli and the grunt teraponjarbua, were pre-exposed to zn either from waterborne (0.74-170 microg l-1) or dietary (39-5926 microg g-1) zn for 1 or 3 weeks. the concentrations of zn and metallothionein (mt) in the whole body of the black sea bream and in the gills, viscera and carcass of the grunt were then measured during this ... | 2005 | 15896856 |
| upregulation of the somatotropic axis is correlated with increased g6pdh expression in black sea bream adapted to iso-osmotic salinity. | black sea bream (mylio macrocephalus) were adapted to salinities of 0 ppt (freshwater), 6 ppt (hypo-osmotic), 12 ppt (iso-osmotic), 33 ppt (seawater), and 50 ppt (hypersaline) for 1 month. using rt-pcr assays, the expression of pituitary growth hormone (gh) and hepatic insulin-like growth factor 1 (igf-1) genes were studied. it was found that the transcripts for both of these genes were highest in fish maintained at iso-osmotic salinity. to correlate the expression of gh and igf-1 with an index ... | 2005 | 15891045 |
| current status of genetic and endocrine factors in the sex change of protandrous black porgy, acanthopagrus schlegeli (teleostean). | black porgy, acanthopagrus schlegeli bleeker, a marine protandrous hermaphrodite fish, is functionally male for the first 2 years of life, but begins to sexually change to female after the third year. testicular tissue and ovarian tissue are separated by connective tissue in the bisexual gonad. this sex pattern provides a unique model to study the mechanism of sex change in fish. the annual profiles of plasma estradiol, vitellogenin, and 11-ketotestosterone concentrations in males were significa ... | 2005 | 15891026 |
| corals have already evolved the vertebrate-type hormone system in the sexual reproduction. | most broadcast spawning scleractinian corals synchronously release gametes during a brief annual spawning period. in southern taiwan, the mass spawning of scleractinians occurs in lunar mid-march. endocrine system has been proposed to play important roles in this annual phenomenon. a scleractinian coral, euphyllia ancora has been selected as a model for the hormones and reproduction studies. we detected the presence of estradiol (e2), testosterone (t), glucuronided e2, glucuronided t, aromatase, ... | 2005 | 20035443 |
| identification of 17,20beta,21-trihydroxy-4-pregnen-3-one as an oocyte maturation-inducing steroid in black porgy, acanthopagrus schlegeli. | the identity of the maturation-inducing steroid (mis) in black porgy, acanthopagrus schlegeli, a marine protandrous teleost, is unknown. previous studies demonstrated that two teleost miss, the progestins 17,20beta,21-trihydroxy-4-pregnen-3-one (20beta-s) and 17,20beta-dihydroxy-4-pregnen-3-one (dhp) can induce maturation of black porgy oocytes in vitro. the purpose of the present study was to identify the major progestin produced during oocyte maturation (om) in black porgy and investigate whet ... | 2005 | 15639146 |
| seasonal profiles of brain and pituitary gonadotropin-releasing hormone and plasma luteinizing hormone in relation to sex change of protandrous black porgy, acanthopagrus schlegeli. | three molecular variants of gnrh in the brain (sbgnrh, sgnrh, and cgnrh-ii) and two forms in the pituitary (sbgnrh and sgnrh) were detected in protandrous black porgy, acanthopagrus schlegeli using chromatographic and immunological methods. in juvenile fish, brain sbgnrh, sgnrh, and cgnrh-ii levels increased in may and reached their highest levels in july and august (the nonspawning season) and in january through march (the spawning season). in fish aged 1 yr and older, high levels of brain sbgn ... | 2005 | 15601925 |
| in vivo and in vitro sex steroids stimulate seabream gonadotropin-releasing hormone content and release in the protandrous black porgy, acanthopagrus schlegeli. | the objective of the present study was to investigate the regulation of seabream gonadotropin-releasing hormone (sbgnrh) release using in vivo and in vitro approaches in the protandrous black porgy, acanthopagrus schlegeli. estradiol-17beta (e2), testosterone (t), and 11-ketotestosterone (11-kt) were found to significantly stimulate the increase of sbgnrh levels in pituitary of black porgy after 5-96 h of injection. an in vitro culture system using dispersed brain neurons was also developed to i ... | 2004 | 15474531 |
| sequence and expression of cytochrome p450 aromatase and ftz-f1 genes in the protandrous black porgy (acanthopagrus schlegeli). | in this study, a cdna encoding cytochrome p450 aromatase (p450arom) was cloned from black porgy acanthopagrus schlegeli ovary. the deduced amino acid sequence had high homology with ovarian p450arom of other teleost fish. moreover, we partially cloned two ftz-f1 homologues (asff1a and asff1b) from black porgy. comparative sequence analysis grouped asff1a and asff1b in nr5a2 and nr5a4 clades, respectively. among the various tissues tested, p450arom mrna was highly expressed in the ovary and weakl ... | 2004 | 15364207 |
| estradiol-17beta induced a reversible sex change in the fingerlings of protandrous black porgy, acanthopagrus schlegeli bleeker: the possible roles of luteinizing hormone in sex change. | the objectives of the present study were to investigate the effects of oral administration of estradiol-17beta (e(2)) on luteinizing hormone (lh) in plasma, aromatase activity in gonad, and sex change in the fingerlings of protandrous black porgy, acanthopagus schlegeli bleeker. the expression of estrogen receptor (er) and androgen receptor (ar) transcripts in gonad was also analyzed. undifferentiated (2-mo-old) black porgy were divided into two groups, one fed a control diet and the other a die ... | 2004 | 15189829 |
| chronic salinity adaptation modulates hepatic heat shock protein and insulin-like growth factor i expression in black sea bream. | black sea bream ( mylio macrocephalus) hepatic heat shock proteins hsp90, hsp70, and hsp60 were found to be thermally and reversibly inducible as they were elevated 2.0, 3.2, and 2.1 fold, respectively, on acute heat shock and returned to pre-heat-shock levels after a 40-hour recovery period. to establish whether salinity plays a role in regulating heat shock protein (hsp) and insulin-like growth factor-i (igf-i) expression in a euryhaline marine fish, we adapted groups of juvenile black sea bre ... | 2002 | 14961280 |
| differential dmrt1 transcripts in gonads of the protandrous black porgy, acanthopagrus schlegeli. | black porgy, acanthopagrus schlegeli bleeker, is a marine protandrous hermaphrodite fish. a dmrt1 cdna was cloned and characterized and in order to study the process of sex change in this species, mrna transcripts of dmrt1 were monitored. dmrt1 was specifically transcribed in testis and seminal vesicle in 2-year-old black porgy according to rt-pcr and southern analysis. a real-time quantification pcr analysis was further developed for the measurement of dmrt1 transcripts. dmrt1 transcripts were ... | 2003 | 14685000 |
| molecular cloning and characterization of gilthead sea bream (sparus aurata) growth hormone receptor (ghr). assessment of alternative splicing. | the full-length growth hormone receptor (ghr) of gilthead sea bream (sparus aurata) was cloned and sequenced by rt-pcr and rapid amplification of 5'and 3'ends. the open reading frame codes for a mature 609 amino acid protein with a hydrophobic transmembrane region and all the characteristic motifs of ghrs. sequence analysis revealed a 96 and 76% of amino acid identity with black sea bream (acanthopagrus schlegeli) and turbot (scophthalmus maximus) ghrs, respectively, but this amino acid identity ... | 2003 | 12941635 |
| differential messenger rna transcription of androgen receptor and estrogen receptor in gonad in relation to the sex change in protandrous black porgy, acanthopagrus schlegeli. | black porgy, acanthopagrus schlegeli bleeker, is a marine protandrous hermaphrodite fish. all are functional males at 1-2 yr of age and then become either males or females at 3 yr of age. to study the process of sex change in this species, mrna transcripts of two estrogen receptors (eralpha and erbeta) and an androgen receptor (ar) were monitored. an ar cdna was cloned and characterized. eralpha, erbeta, and ar were differentially transcribed in bisexual testicular and ovarian tissue according t ... | 2003 | 12672663 |