| kocuria palustris sp. nov. and kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (typha angustifolia). | two gram-positive, aerobic spherical actinobacteria were isolated from the rhizoplane of narrow-leaved cattail (typha angustifolia) collected from a floating mat in the soroksár tributary of the danube river, hungary. sequence comparisons of the 16s rdna indicated these isolates to be phylogenetic neighbours of members of the genus kocuria, family micrococcaceae, in which they represent two novel lineages. the phylogenetic distinctness of the two organisms ta68t and taga27t was supported by dna- ... | 1999 | 10028258 |
| kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample. | strain cms 76ort, an orange-pigmented bacterium, was isolated from a cyanobacterial mat sample from a pond located in mcmurdo dry valley, antarctica. on the basis of chemotaxonomic and phylogenetic properties, strain cms 76ort was identified as a member of the genus kocuria. it exhibited a 16s rdna similarity of 99.8% and dna-dna similarity of 71% with kocuria rosea (atcc 186t). phenotypic traits confirmed that strain cms 78ort and k. rosea were well differentiated. furthermore, strain cms 76ort ... | 2003 | 12656171 |
| reclassification of atcc 9341 from micrococcus luteus to kocuria rhizophila. | strain atcc 9341, currently known as micrococcus luteus, has been designated as a quality-control strain in a number of applications. it is also cited as the standard culture in several official methods and manuals, as well as the code of federal regulations. over the years, it has become apparent that atcc 9341 does not resemble other m. luteus strains; however, its phenotypic characteristics alone were ambiguous. recently, a polyphasic study was performed in which molecular data were combined ... | 2003 | 12892116 |
| comparison of various assays used for detection of beta-lactam antibiotics in poultry meat. | in this study, microbiological tests for the detection of beta-lactam antibiotics in meat and meat products were evaluated. the traditional fpt (four plate test, containing bacillus subtilis and kocuria rhizophila), bsda (bacillus stearothermophilus disc assay) and a newly developed microbiological test, premi test (containing bacillus stearothermophilus) were included in the study. the limit of detection (lod) of the premi test was compared with the lod of the traditional methods. the detection ... | 2005 | 16019829 |
| inhibitory effect of oxalic acid on bacterial spoilage of raw chilled chicken. | oxalic acid was evaluated as a treatment for reducing populations of naturally occurring microorganisms on raw chicken. raw chicken breasts were dipped in solutions of oxalic acid (0, 0.5, 1.0, 1.5, and 2.0%, wt/vol) for 10, 20, and 30 min, individually packed in oxygen-permeable polyethylene bags, and stored at 4 degrees c. total plate counts of aerobic bacteria and populations of pseudomonas spp. and enterobacteriaceae on breasts were determined before treatment and after storage for 1, 3, 7, ... | 2006 | 16924917 |
| biodiversity of bacterial ecosystems in traditional egyptian domiati cheese. | bacterial biodiversity occurring in traditional egyptian soft domiati cheese was studied by pcr-temporal temperature gel electrophoresis (ttge) and pcr-denaturing gradient gel electrophoresis (dgge). bands were identified using a reference species database (j.-c. ogier et al., appl. environ. microbiol. 70:5628-5643, 2004); de novo bands having nonidentified migration patterns were identified by dna sequencing. results reveal a novel bacterial profile and extensive bacterial biodiversity in domia ... | 2007 | 17189434 |
| water sensitivity, antimicrobial, and physicochemical analyses of edible films based on hpmc and/or chitosan. | several properties of chitosan films associated or not with hydroxypropylmethylcellulose polymer (hpmc) and hpmc films incorporating or not nisin and/or milk fat were studied. nisin addition at a level of 250 microg ml-1 and likewise chitosan at 1% (w/v) concentration were efficient for total inhibiting aspergillus niger and kocuria rhizophila food deterioration microorganisms. hpmc and chitosan films were transparent, whereas nisin and/or fat incorporation induced a 2-fold lightness parameter i ... | 2007 | 17263462 |
| complete genome sequence of the soil actinomycete kocuria rhizophila. | the soil actinomycete kocuria rhizophila belongs to the suborder micrococcineae, a divergent bacterial group for which only a limited amount of genomic information is currently available. k. rhizophila is also important in industrial applications; e.g., it is commonly used as a standard quality control strain for antimicrobial susceptibility testing. sequencing and annotation of the genome of k. rhizophila dc2201 (nbrc 103217) revealed a single circular chromosome (2,697,540 bp; g+c content of 7 ... | 2008 | 18408034 |
| kocuria rhizophila adds to the emerging spectrum of micrococcal species involved in human infections. | we describe the first case of a kocuria rhizophila infection in a boy with methylmalonic aciduria. a single clone was isolated from blood samples drawn through a port system and from peripheral veins during septic episodes within a 2-year period. k. rhizophila expands the emerging number of "micrococci" considered to be etiologically relevant. | 2008 | 18614658 |
| evaluation of a microbiological multi-residue system on the detection of antibacterial substances in ewe milk. | to protect both, public health and the dairy industry, from the presence of antibiotic residues in milk, control programmes have been established, which include the needed screening tests. this work focuses on the application of a microbiological multi-residue system in ewe milk, a method based on the use of six different plates, each seeded with one of the following bacteria: geobacillus stearothermophilus var. calidolactis (beta-lactams), bacillus subtilis at ph 8.0 (aminoglycosides), kocuria ... | 2009 | 19100896 |
| in vitro evaluation of antibacterial, anticollagenase, and antioxidant activities of hop components (humulus lupulus) addressing acne vulgaris. | seven naturally derived components from hop plant (humulus lupulus l.) extracts were tested for evaluation of biological activities affecting acne vulgaris. five strains, propionibacterium acnes, staphylococcus epidermidis, staphylococcus aureus, kocuria rhizophila and, staphylococcus pyogenes, were selected as the main acne-causing bacteria. hop extracts xanthohumol and the lupulones showed strong inhibitory activities against all of the strains. although hydrogenated derivatives did not show t ... | 2009 | 19201179 |
| isolation of bacterial strains from bovine fecal microflora capable of degradation of ceftiofur. | ceftiofur, a third-generation cephalosporin used to treat bacterial infections in animals, is degraded in bovine feces but the specific bacteria involved are unknown. to find the bacteria involved in ceftiofur metabolism, the bovine fecal microflora was screened. twenty-one nonidentical strains of bovine fecal bacteria were isolated on media containing 1-32 microg ml(-1) of ceftiofur. the cultures were incubated with 5 microg ml(-1) ceftiofur for different times, then centrifuged and analyzed by ... | 2009 | 19428193 |
| kocuria atrinae sp. nov., isolated from traditional korean fermented seafood. | a novel actinobacterium, strain p30(t), was isolated from jeotgal, a traditional korean fermented seafood. cells were aerobic, gram-positive, non-motile and coccoid. optimal growth occurred at 30-37 degrees c, at ph 8-9 and in the presence of 0-2 % (w/v) nacl. based on 16s rrna gene sequence analysis, strain p30(t) was phylogenetically closely related to kocuria carniphila, kocuria gwangalliensis, kocuria rhizophila, kocuria marina, kocuria rosea and k. varians with levels of similarity of 98.6, ... | 2010 | 19661502 |
| antimicrobial efficiency of titanium dioxide-coated surfaces. | development and evaluation of an antimicrobially active titanium dioxide coating. | 2010 | 19886892 |
| genome sequence of the fleming strain of micrococcus luteus, a simple free-living actinobacterium. | micrococcus luteus (nctc2665, "fleming strain") has one of the smallest genomes of free-living actinobacteria sequenced to date, comprising a single circular chromosome of 2,501,097 bp (g+c content, 73%) predicted to encode 2,403 proteins. the genome shows extensive synteny with that of the closely related organism, kocuria rhizophila, from which it was taxonomically separated relatively recently. despite its small size, the genome harbors 73 insertion sequence (is) elements, almost all of which ... | 2010 | 19948807 |
| genes involved in long-chain alkene biosynthesis in micrococcus luteus. | aliphatic hydrocarbons are highly appealing targets for advanced cellulosic biofuels, as they are already predominant components of petroleum-based gasoline and diesel fuels. we have studied alkene biosynthesis in micrococcus luteus atcc 4698, a close relative of sarcina lutea (now kocuria rhizophila), which 4 decades ago was reported to biosynthesize iso- and anteiso-branched, long-chain alkenes. the underlying biochemistry and genetics of alkene biosynthesis were not elucidated in those studie ... | 2010 | 20038703 |
| antibacterial activity of root canal filling materials for primary teeth: zinc oxide and eugenol cement, calen paste thickened with zinc oxide, sealapex and endorez. | this study evaluated in vitro the antibacterial activity of 4 root canal filling materials for primary teeth - zinc oxide and eugenol cement (zoe), calen paste thickened with zinc oxide (calen/zo), sealapex sealer and endorez sealer - against 5 bacterial strains commonly found in endodontic infections (kocuria rhizophila, enterococcus faecalis, streptococcus mutans, escherichia coli and staphylococcus aureus) using the agar diffusion test (agar-well technique). calen paste, 1% chlorhexidine digl ... | 2009 | 20069251 |
| kocuria salsicia sp. nov., isolated from salt-fermented seafood. | strain 104(t) was isolated from a traditional salt-fermented seafood in korea. it was a gram-positive, non-motile, coccus-shaped bacterium. it formed lemon-yellow, opaque colonies that were circular with entire margins. optimal growth occurred at 30-37 °c, ph 7-8 and in the presence of 0-2 % (w/v) nacl. phylogenetic analysis of 16s rrna gene sequences from strain 104(t) and reference species of the genus kocuria indicated that strain 104(t) formed an independent line. the g+c content of the chro ... | 2011 | 20228215 |
| inhibitory effects of lactic acid and lauricidin on spoilage organisms of chicken breast during storage at chilled temperature. | different concentrations of lauricidin (lu, containing 1% lactic acid) and lactic acid alone (la) were evaluated for their effectiveness in reducing naturally occurring microflora of raw chicken breasts. chicken breasts were dipped in 0 (control), 0.5, 1.0, 1.5, and 2.0% solutions of lu (w/v) or la (v/v) for 10, 20, and 30 min and stored at 4°c for 14 d. total plate counts (tpc) and populations of pseudomonas spp. and enterobacteriaceae were determined before and after dipping and after storing ... | 2010 | 20947197 |
| comparative bioremediation potential of four rhizospheric microbial species against lindane. | four microbial species (kocuria rhizophila, microbacterium resistens, staphylococcus equorum and staphylococcus cohnii subspecies urealyticus) were isolated from the rhizospheric zone of selected plants growing in a lindane contaminated environment and acclimatized in lindane spiked media (5-100 μg ml⁻¹). the isolated species were inoculated with soil containing 5, 50 and 100 mg kg⁻¹ of lindane and incubated at room temperature. soil samples were collected periodically to evaluate the microbial ... | 2010 | 21044795 |
| isolation and identification of soil bacteria growing at the expense of arbuscular mycorrhizal fungi. | soil-microorganism symbioses are of fundamental importance for plant adaptation to the environment. research in microbial ecology has revealed that some soil bacteria are associated with arbuscular mycorrhizal fungi (amf). however, these interactions may be much more complex than originally thought. to assess the type of bacteria associated with amf, we initially isolated spores of glomus irregulare from an agrostis stolonifera rhizosphere. the spores were washed with sterile water and plated on ... | 2011 | 21219415 |
| detection, selective isolation and characterisation of dactylosporangium strains from diverse environmental samples. | a culture-independent, nested pcr procedure based on genus-specific oligonucleotide primers detected the presence of members of the genus dactylosporangium in 14 out of 21 diverse environmental samples. clones generated from the 14 positive environmental samples formed distinct phyletic lines in the dactylosporangial 16s rrna gene tree. presumptive dactylosporangiae were isolated from 7 of these samples using a medium designed to be selective for members of the genus dactylosporangium, namely st ... | 2011 | 21596505 |
| draft genome sequence of kocuria rhizophila p7-4. | we report the draft genome sequence of kocuria rhizophila p7-4, which was isolated from the intestine of siganus doliatus caught in the pacific ocean. the 2.83-mb genome sequence consists of 75 large contigs (>100 bp in size) and contains 2,462 predicted protein-coding genes. | 2011 | 21685281 |
| microbial production of aliphatic (s)-epoxyalkanes by using rhodococcus sp. strain st-10 styrene monooxygenase expressed in organic-solvent-tolerant kocuria rhizophila dc2201. | we describe the development of biocatalysis for producing optically pure straight-chain (s)-epoxyalkanes using styrene monooxygenase of rhodococcus sp. strain st-10 (rhsmo). rhsmo was expressed in the organic solvent-tolerant microorganism kocuria rhizophila dc2201, and the bioconversion reaction was performed in an organic solvent-water biphasic reaction system. the biocatalytic process enantioselectively converted linear terminal alkenes to their corresponding (s)-epoxyalkanes using glucose an ... | 2015 | 25556188 |
| kocuria rhizophila misidentified as corynebacterium jeikeium and other errors caused by the vitek ms system call for maintained microbiological competence in the era of matrix-assisted laser desorption ionization-time of flight mass spectrometry. | | 2014 | 25392349 |
| a new ascochlorin derivative from cylindrocarpon sp. fki-4602. | cylindrol a₅, a new ascochlorin congener, was isolated along with 14 known compounds from the culture broth of cylindrocarpon sp. fki-4602 by solvent extraction, octadecylsilane column chromatography and hplc. the structure of cylindrol a₅ was elucidated by spectral analyses, including nmr. the compound has an ascochlorin skeleton consisting of a resorcin aldehyde and a cyclohexanone moieties. cylindrol a₅ showed moderate antimicrobial activity against bacillus subtilis, kocuria rhizophila, myco ... | 2013 | 23168404 |
| validation of the bact/alert(r) 3d system for rapid sterility testing of biopharmaceutical samples. | the bact/alert® 3d system was validated to determine the sterility of different types of biopharmaceutical samples such as water for injection, unprocessed bulk, and finished bulk. the installation, operation, and performance qualification were completed and verified under good manufacturing practices. during the installation and operation validation stages, the functionality and security of the system and software were completed and verified. for the performance qualification, 11 microorganisms ... | 2016 | 22307661 |
| iminimycin a, the new iminium metabolite produced by streptomyces griseus os-3601. | a new natural product, designated iminimycin a, was isolated from the cultured broth of a streptomycin-producing microbial strain, streptomyces griseus os-3601, via a physicochemical screening method using hp-20, silica gel and ods column chromatographies and subsequent preparative hplc. iminimycin a is an indolizidine alkaloid, containing of an unusual iminium group and a cyclopropane ring with a triene side chain. the absolute configuration of iminimycin a was elucidated by nmr studies and ele ... | 2016 | 26758492 |
| identification and antimicrobial activity of actinobacteria from soils in southern thailand. | the aim of this research was to study on the identification and antimicrobial activity of actinobacteria from six soil samples collected around krung ching waterfall, nakhon si thammarat province, the southern part of thailand. thirty-one isolates of actinobacteria were isolated using the dilution plating method on modified starch casein nitrate agar plates and potato starch-glycerol agar plates. on the primary screening, 9 isolates exhibited the antimicrobial activity against bacillus subtilis, ... | 2013 | 23665707 |
| green synthesis of silver nanoparticles, their characterization, application and antibacterial activity. | our research focused on the production, characterization and application of silver nanoparticles (agnps), which can be utilized in biomedical research and environmental cleaning applications. we used an environmentally friendly extracellular biosynthetic technique for the production of the agnps. the reducing agents used to produce the nanoparticles were from aqueous extracts made from the leaves of various plants. synthesis of colloidal agnps was monitored by uv-visible spectroscopy. the uv-vis ... | 2013 | 24157517 |
| use of nanoparticulate zinc oxide as intracanal medication in endodontics: ph and antimicrobial activity. | the aim of this study was to evaluate the ph and antimicrobial activity of micro or nanoparticulate zinc oxide (zno) pastes with or without calcium hydroxide (ch). the following medications were evaluated: microparticulate zno + polyethylene glycol (peg) 400; nanoparticulate zno + peg 400; peg 400; ch + microparticulate zno + peg 400 and ch + nanoparticulate zno + peg 400. the ph was assessed between 12 hours and 28 days, using a digital ph meter. the antimicrobial activity against enterococcus ... | 2013 | 25335366 |
| impact of nisin-activated packaging on microbiota of beef burgers during storage. | beef burgers were stored at 4°c in a vacuum in nisin-activated antimicrobial packaging. microbial ecology analyses were performed on samples collected between days 0 and 21 of storage to discover the population diversity. two batches were analyzed using rna-based denaturing gradient gel electrophoresis (dgge) and pyrosequencing. the active packaging retarded the growth of the total viable bacteria and lactic acid bacteria. culture-independent analysis by pyrosequencing of rna extracted directly ... | 2015 | 26546424 |
| characteristics of microbial biofilm on wooden vats ('gerles') in pdo salers cheese. | the purpose of this study was to characterize microbial biofilms from 'gerles' (wooden vats for making pdo salers cheese) and identify their role in milk inoculation and in preventing pathogen development. gerles from ten farms producing pdo salers cheese were subjected to microbial analysis during at least 4 periods spread over two years. they were distinguished by their levels of lactobacillus (between 4.50 and 6.01 log cfu/cm(2)), gram negative bacteria (between 1.45 and 4.56 log cfu/cm(2)), ... | 2012 | 22483401 |
| chemical composition and antimicrobial activity of the essential oils of onychopetalum amazonicum r.e.fr. | the essential oils from leaves, twigs and trunk bark of onychopetalum amazonicum r.e. fr. (annonaceae), obtained by hydrodistillation, were analysed by gc and gc-ms, and also were evaluated for in vitro antimicrobial activity. forty-one compounds, which correspond to 75.0-92.2% of the oil components, were identified. major compounds were sesquiterpenes, including (e)-caryophyllene, caryophyllene oxide, spathulenol, α-gurjunene, allo-aromadendrene and α-epi-cadinol. the oils were evaluated for an ... | 2016 | 27033169 |
| polysaccharide-based silver nanoparticles synthesized by klebsiella oxytoca dsm 29614 cause dna fragmentation in e. coli cells. | silver nanoparticles (agnps), embedded into a specific exopolysaccharide (eps), were produced by klebsiella oxytoca dsm 29614 by adding agno3 to the cultures during exponential growth phase. in particular, under aerobic or anaerobic conditions, two types of silver nanoparticles, named agnps-eps(aer) and the agnps-eps(anaer), were produced respectively. the effects on bacterial cells was demonstrated by using escherichia coli k12 and kocuria rhizophila atcc 9341 (ex micrococcus luteus) as gram-ne ... | 2016 | 26886276 |
| actinomadurol, an antibacterial norditerpenoid from a rare actinomycete, actinomadura sp. kc 191. | a new secondary metabolite, actinomadurol (1), was isolated along with the known compound jbir-65 (2) from a rare actinomycete, actinomadura strain kc 191. the structure of 1 was established as a rare member of the bacterial c-19 norditerpenoid class by nmr data and ecd calculations. the absolute configuration of 2, which was previously reported without stereochemical analysis, was determined by using the modified mosher's method and ecd calculations. actinomadurol (1) exhibited potent antibacte ... | 2016 | 27367579 |
| draft genome sequence of kocuria rhizophila strain tpw45, an actinobacterium isolated from freshwater. | kocuria rhizophila is a ubiquitous bacterium which is well known for its industrial value. here, we present the draft genome of kocuria rhizophila strain tpw45 which was isolated from sungai gabai, selangor, malaysia. the assembled genome comprised of 46 contigs and the estimated genome size is 2.7 mb. based on the rast annotation, a gene cluster responsible for aromatic compound degradation was identified in this strain. | 2016 | 27326264 |
| draft genome sequence of kocuria rhizophila rf, a radiation-resistant soil isolate. | kocuria rhizophila rf, a soil isolate from iran, is a radiation-resistant bacterium. only a limited amount of genomic information for radiation-resistant bacteria is currently available. here, we report the draft genome sequence of this bacterium, providing knowledge to aid in the discovery of the genomic basis of its resistance to radiation. | 2016 | 26966202 |
| kocuria arsenatis sp. nov., a novel arsenic-resistant endophytic actinobacterium associated with prosopis laegivata grown on high arsenic polluted mine tailing in mexico. | a gram positive, aerobic, non-motile, coccoid, arsenic-resistant actinobacterial strain designated as cm1e1t was isolated from the lateral root tissue of prosopis laegivata grown on a mine tailing in san luis potosi, mexico. phylogenetic analysis based on 16s rrna genes revealed that strain cm1e1t was clustered closely with kocuria species, showing the highest sequence similarity of 98.7 % to kocuria rhizophila ta68t. the dna g + c content of strain cm1e1t was 74.5 mol% (tm). the major fatty aci ... | 2015 | 26647141 |
| characterization of a novel strain phylogenetically related to kocuria rhizophila and its chemical modification to improve performance of microbial fuel cells. | it is certainly an important research area to discovery new exoelectrogens for microbial fuel cells (mfcs), and how to effectively manipulate its cell property to improve power performance is still a great challenge. in this study, a new electrochemically active bacterium phylogenetically related to kocuria rhizophila was first isolated and found electrogenic in mfcs, which was identified through the combination methods of molecular biology, physiological, biochemical and morphological character ... | 2015 | 25721974 |
| persistent bloodstream infection with kocuria rhizophila related to a damaged central catheter. | a case of persistent bloodstream infection with kocuria rhizophila related to a damaged central venous catheter in a 3-year-old girl with hirschsprung's disease is reported. the strain was identified as k. rhizophila by 16s rrna gene sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry. arbitrarily primed pcr analysis showed a clonal strain. the repeated septic episodes were resolved with the catheter repair. | 2012 | 22259211 |