kocuria palustris sp. nov. and kocuria rhizophila sp. nov., isolated from the rhizoplane of the narrow-leaved cattail (typha angustifolia).two gram-positive, aerobic spherical actinobacteria were isolated from the rhizoplane of narrow-leaved cattail (typha angustifolia) collected from a floating mat in the soroksár tributary of the danube river, hungary. sequence comparisons of the 16s rdna indicated these isolates to be phylogenetic neighbours of members of the genus kocuria, family micrococcaceae, in which they represent two novel lineages. the phylogenetic distinctness of the two organisms ta68t and taga27t was supported by dna- ...199910028258
kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample.strain cms 76ort, an orange-pigmented bacterium, was isolated from a cyanobacterial mat sample from a pond located in mcmurdo dry valley, antarctica. on the basis of chemotaxonomic and phylogenetic properties, strain cms 76ort was identified as a member of the genus kocuria. it exhibited a 16s rdna similarity of 99.8% and dna-dna similarity of 71% with kocuria rosea (atcc 186t). phenotypic traits confirmed that strain cms 78ort and k. rosea were well differentiated. furthermore, strain cms 76ort ...200312656171
isolation and characterization of bacteria resistant to metallic copper surfaces.metallic copper alloys have recently attracted attention as a new antimicrobial weapon for areas where surface hygiene is paramount. currently it is not understood on a molecular level how metallic copper kills microbes, but previous studies have demonstrated that a wide variety of bacteria, including escherichia coli, staphylococcus aureus, and clostridium difficile, are inactivated within minutes or a few hours of exposure. in this study, we show that bacteria isolated from copper alloy coins ...201020048058
description of a novel actinobacterium kocuria assamensis sp. nov., isolated from a water sample collected from the river brahmaputra, assam, india.a gram-positive, pale yellow pigmented actinobacterium, strain s9-65(t) was isolated from a water sample collected from the river brahmaputra, assam, india and subjected to a polyphasic taxonomic study. the physiological and biochemical properties, major fatty acids (anteiso-c15:0 and anteiso-c17:0), estimated dna g+c content (69.2 mol %) and 16s rrna gene sequence analysis showed that strain s9-65(t) belonged to the genus kocuria. strain s9-65(t) exhibited highest 16s rrna gene sequence similar ...201121234681
the microbiota of an unpolluted calcareous soil faces up chlorophenols: evidences of resistant strains with potential for highlight the effects of a variety of chlorophenols (cp) in relation to the response of an indigenous bacterial community, an agricultural mediterranean calcareous soil has been studied in microcosms incubated under controlled laboratory conditions. soil samples were artificially polluted with 2-monochlorophenol (mcp), 2,4,6-trichlorophenol (tcp) and pentachlorophenol (pcp), at concentrations ranging from 0.1 up to 5000 mg kg(-1). both activity and composition of the microbial community were ...201121295817
rice seeds as sources of endophytic bacteria.endophytic bacteria are considered to originate from the external environment. to examine the hypothesis that rice (oryza sativa, cultivar kinuhikari) seeds are a source of endophytic bacteria, we isolated endophytic bacteria from the shoots, remains of the seeds, and roots of rice seedlings that were aseptically cultivated in vitro from surface-disinfected seeds. of the various bacterial strains isolated, the closest relatives, identified by 16s rrna gene sequencing, were: bacillus firmus, b. f ...200921566368
genetic diversity and phylogeny of antagonistic bacteria against phytophthora nicotianae isolated from tobacco rhizosphere.the genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by boxair-pcr, 16s-rflp, 16s rrna sequence homology and phylogenetic analysis methods. these studies revealed that 4.01% of the 6652 tested had some inhibitory activity against phytophthora nicotianae. boxair-pcr analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. a total of 25 16s-rflp patterns were iden ...201121686169
Two-phase olive mill waste composting: community dynamics and functional role of the resident microbiota.In this study, physico-chemical modifications and community dynamics and functional role of the resident microbiota during composting of humid husk from a two-phase extraction system (TPOMW) were investigated. High mineralization and humification of carbon, low loss of nitrogen and complete degradation of polyphenols led to the waste biotransformation into a high-quality compost. Viable cell counts and denaturing gradient gel electrophoresis (DGGE) profiling of the 16S rRNA genes showed that the ...201121996482
kocurin, the true structure of pm181104, an anti-methicillin-resistant staphylococcus aureus (mrsa) thiazolyl peptide from the marine-derived bacterium kocuria palustris.a new thiazolyl peptide, kocurin (1), was isolated from culture broths of a marine-derived kocuria palustris. its structural elucidation was accomplished using a combination of spectroscopic and chemical methods, including hrms, extensive 1d and 2d nmr analysis, ms/ms fragmentation, and chemical degradation and marfey's analysis of the resulting amino acid residues. the structure herein reported corrects that previously assigned to pm181104 (3). kocurin displayed activity against methicillin-res ...201323380989
biodegradability of commercial and weathered diesel oils.this work aimed to evaluate the capability of different microorganisms to degrade commercial diesel oil in comparison to a weathered diesel oil collected from the groundwater at a petrol station. two microbiological methods were used for the biodegradability assessment: the technique based on the redox indicator 2,6 -dichlorophenol indophenol (dcpip) and soil respirometric experiments using biometer flasks. in the former we tested the bacterial cultures staphylococcus hominis, kocuria palustris, ...200824031193
diversity of pigmented gram-positive bacteria associated with marine macroalgae from antarctica.little is known about the diversity and roles of gram-positive and pigmented bacteria in antarctic environments, especially those associated with marine macroorganisms. this work is the first study about the diversity and antimicrobial activity of culturable pigmented gram-positive bacteria associated with marine antarctic macroalgae. a total of 31 pigmented gram-positive strains were isolated from the surface of six species of macroalgae collected in the king george island, south shetland islan ...201526507390
biodegradation of low-density polyethylene by marine bacteria from pelagic waters, arabian sea, india.sixty marine bacteria isolated from pelagic waters were screened for their ability to degrade low-density polyethylene; among them, three were positive and able to grow in a medium containing polythene as the sole carbon source. the positive isolates were identified as kocuria palustris m16, bacillus pumilus m27 and bacillus subtilis h1584 based on the 16s rrna gene sequence homology. the weight loss of polyethylene was 1%, 1.5% and 1.75% after 30 days of incubation with the m16, m27 and h1584 i ...201324210946
genome sequence of kocuria palustris strain cd07_3 isolated from the duodenal mucosa of a celiac disease patient.we report here the 2.8-mb genome of kocuria palustris strain cd07_3 isolated from the duodenal mucosa of a celiac disease (cd) patient. the genome of the bacterium consists of specific virulence factor genes and antibiotic resistance genes that depict its pathogenic potential.201627125478
genome sequence of kocuria palustris strain w4.we report the 3.09 mb draft genome sequence ofkocuria palustrisw4, isolated from a slaughterhouse in denmark.201627034478
determination of the residual anthracene concentration in cultures of haloalkalitolerant actinomycetes by excitation fluorescence, emission fluorescence, and synchronous fluorescence: comparative study.polycyclic aromatic hydrocarbons (pahs) are compounds that can be quantified by fluorescence due to their high quantum yield. haloalkalitolerant bacteria tolerate wide concentration ranges of nacl and ph. they are potentially useful in the pahs bioremediation of saline environments. however, it is known that salinity of the sample affects fluorescence signal regardless of the method. the objective of this work was to carry out a comparative study based on the sensitivity, linearity, and detectio ...201626925294
complete genome sequence of kocuria palustris mu14/1.presented here is the first completely assembled genome sequence of kocuria palustris, an actinobacterial species with broad ecological distribution. the single, circular chromosome of k. palustris mu14/1 comprises 2,854,447 bp, has a g+c content of 70.5%, and contains a deduced gene set of 2,521 coding sequences.201526472837
keratitis with kocuria palustris and rothia mucilaginosa in vitamin a present a case of unusual corneal infection early in the course of peripheral ulcerative keratitis in a patient with severe vitamin a deficiency.201424707276
draft genome sequence of kocuria palustris pel.we report the 2.9-mb draft genome sequence of an actinobacterium, kocuria palustris pel, of the family micrococcaceae.201424504000
[detoxification of high concentrations of trinitrotoluene by bacteria].the ability of the strains-destructors of various aromatic compounds to utilize trinitrotoluene (tnt) up to concentration of 70 mg/1 was shown. an increase in the tnt concentration from 100 to 150 mg/1 did not inhibit its conversion rate by the kocuria palustris rs32 strain. the acinetobacter sp. vt 11 strain utilized tnt as a sole substrate for growth; 3,5-dinitro-4-methyl anilide acetate and 2,6-dinitro-4-aminotoluene were identified as intermediates of tnt degradation by active strains of pse ...201222567882
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