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a new alui satellite dna in the root-knot nematode meloidogyne fallax: relationships with satellites from the sympatric species m. hapla and m. chitwoodi.a highly abundant satellite dna comprising 20% of the meloidogyne fallax (nematoda, tylenchida) genome was cloned and sequenced. the satellite monomer is 173 bp long and has a high a + t content of 72.3%, with frequent runs of a's and t's. the sequence variability of the monomers is 2.7%, mainly due to random distribution of single-point mutations. a search for evidence of internal repeated subunits in the monomer sequence revealed a 6-bp motif (aaattt) for which five degenerated repeats, differ ...19989729876
a major gene mapped on chromosome xii is the main factor of a quantitatively inherited resistance to meloidogyne fallax in solanum sparsipilum.meloidogyne fallax is an emerging pest in europe and represents a threat for potato production. we report the mapping of genetic factors controlling a quantitative resistance against m. fallax identified in the solanum sparsipilum genotype 88s.329.15. when infected, this genotype develops a necrotic reaction at the feeding site of the juveniles and totally prevents their development to the female stage. a "f1" diploid progeny consisting of 128 individuals was obtained using the potato (s. tubero ...200616365760
morphological and molecular characteristics of a new species of pasteuria parasitic on meloidogyne ardenensis.a species of the hyper-parasitic bacterium pasteuria was isolated from the root-knot nematode meloidogyne ardenensis infecting the roots of ash (fraxinus excelsior). it is morphologically different from some other pasteuria pathogens of nematodes in that the spores lack a basal ring on the ventral side of the spore and have a unique clumping nature. transmission electron microscopy (tem) showed that the clumps of spores are not random aggregates but result from the disintegration of the suicide ...200717399736
optimization of metam sodium application by rotary spading injection.in the netherlands the only chemical alternative for methyl bromide permitted is an application of metam-sodium (ms) with the active ingredient methyl isothiocyanate (mit). after introduction of a new application method with 'rotary spading injection' legislation restricted the application of ms in 1993 to once in four years and since 2001 once in five years. efficacy after injection of metam sodium at 10 cm depth and rotary spading a 25 cm soil layer was much better than with shank injection at ...200718399505
an early record of meloidogyne fallax from ireland.root-knot nematodes, meloidogyne spp., cause huge economic losses worldwide. currently, three meloidogyne spp. are present on the quarantine a2 list of eppo, meloidogyne chitwoodi, meloidogyne fallax and meloidogyne enterolobii. as a quarantine organism, meloidogyne fallax has been detected in england and northern ireland on sport turf in 2011, and in england on leek in 2013. however, its presence in ireland has probably been overlooked since 1965, when mr. john f. moore and dr. mary t. franklin ...201728144174
dna barcoding for biosecurity: case studies from the uk plant protection program.since its conception, dna barcoding has seen a rapid uptake within the research community. nevertheless, as with many new scientific tools, progression towards the point of routine deployment within diagnostic laboratories has been slow. in this paper, we discuss the application of dna barcoding in the defra plant health diagnostic laboratories, where dna barcoding is used primarily for the identification of invertebrate pests. we present a series of case studies that demonstrate the successful ...201627792411
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