| pcr-based detection of the causal agent of watermark disease in willows (salix spp.) | the watermark disease, caused by brenneria salicis (formerly erwinia salicis), is of significant concern wherever tree-forming willows are grown or occur naturally. the movement of infected, asymptomatic cuttings is a major cause of pathogen dispersal. a reliable and sensitive diagnostic procedure is necessary for the safe movement of willow planting material. we derived primers from the nucleotide sequence of the 16s rrna gene of b. salicis for the development of a pcr to detect this pathogen. ... | 1998 | 9758827 |
| phylogenetic position of phytopathogens within the enterobacteriaceae. | the almost complete 16s rdna sequences of twenty nine plant-associated strains, representing species of the genera erwinia, pantoea and enterobacter were determined and compared with those of other members of the enterobacteriaceae. the species of the genus erwinia may be divided into three phylogenetic groups. cluster i represents the true erwinias and comprises e. amylovora, e. mallotivora, e. persicinus, e. psidii, e. rhapontici and e. tracheiphila. we propose to unite the species of cluster ... | 1998 | 9779605 |
| reclassification of non-pigmented erwinia herbicola strains from trees as erwinia billingiae sp. nov. | twenty-two erwinia-like strains, isolated from trees since the late fifties and belonging to a distinct phenotypic group with resemblance to pantoea agglomerans, were further characterized by conventional biochemical tests, the biolog metabolic fingerprinting system and fatty acid analysis. their phylogenetic positions were determined by comparing the 16s rrna gene sequence of a representative strain to available sequences of erwinia, pantoea, pectobacterium and brenneria species. the strains we ... | 1999 | 10319458 |
| the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae. | the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ... | 1999 | 10555323 |
| phylogenetic relationships of necrogenic erwinia and brenneria species as revealed by glyceraldehyde-3-phosphate dehydrogenase gene sequences. | recent examination of the relationships of the dry necrosis-inducing (necrogenic) erwinias using 16s rdna sequences demonstrated that these bacteria comprise a polyphyletic group and, therefore, have been subdivided into three distinct genera, erwinia, brenneria and pectobacterium, with the classical 'amylovora' group species now being distributed nearly evenly among the first two. to further assess the molecular evolutionary relationships between current necrogenic erwinia and brenneria species ... | 2000 | 11155980 |
| samsonia erythrinae gen. nov., sp. nov., isolated from bark necrotic lesions of erythrina sp., and discrimination of plant-pathogenic enterobacteriaceae by phenotypic features. | bacterial strains isolated from diseased erythrina (erythrina sp.) trees in martinique (french west indies) were studied using phenotypic tests, 16s rdna sequence analysis and dna-dna hybridization. numerical analysis of phenotypic characteristics showed that these strains formed an homogeneous phenon among plant-pathogenic enterobacteriaceae, and gave useful and updated information for the identification of these bacteria. results of dna-dna hybridization indicated that strains from erythrina b ... | 2001 | 11491325 |
| elevation of three subspecies of pectobacterium carotovorum to species level: pectobacterium atrosepticum sp. nov., pectobacterium betavasculorum sp. nov. and pectobacterium wasabiae sp. nov. | a collection of 42 strains belonging to the five subspecies of pectobacterium carotovorum (subspecies atrosepticum, betavasculorum, carotovorum, odoriferum and wasabiae) and 11 reference and type strains of biovars of pectobacterium chrysanthemi, pectobacterium cacticidum and brenneria paradisiaca were studied by dna-dna hybridization, numerical taxonomy of 120 phenotypic characteristics, serology and new phylogenetic analysis of previously reported sequences from a database of aligned 16s rdna ... | 2003 | 12710602 |
| transfer of pectobacterium chrysanthemi (burkholder et al. 1953) brenner et al. 1973 and brenneria paradisiaca to the genus dickeya gen. nov. as dickeya chrysanthemi comb. nov. and dickeya paradisiaca comb. nov. and delineation of four novel species, dickeya dadantii sp. nov., dickeya dianthicola sp. nov., dickeya dieffenbachiae sp. nov. and dickeya zeae sp. nov. | a collection of 75 strains of pectobacterium chrysanthemi (including all biovars and pathovars) and the type strains of brenneria paradisiaca (cfbp 4178(t)) and pectobacterium cypripedii (cfbp 3613(t)) were studied by dna-dna hybridization, numerical taxonomy of 121 phenotypic characteristics, serology and 16s rrna gene-based phylogenetic analyses. from analysis of 16s rrna gene sequences, it was deduced that p. chrysanthemi strains and b. paradisiaca cfbp 4178(t) formed a clade distinct from th ... | 2005 | 16014461 |
| watermark disease in willow and its relation to brenneria salicis dominance in the wood. | | 2005 | 16637165 |
| relationships of plant pathogenic enterobacteria based on partial atpd, cara, and reca as individual and concatenated nucleotide and peptide sequences. | relationships of the genera in the enterobacteriaceae containing plant pathogenic species: brenneria, dickeya, enterobacter, erwinia, pantoea, pectobacterium, and samsonia, were investigated by comparison of their nucleotide and peptide sequences of atpd, cara, reca, and the concatenated sequences. erwinia spp. and pantoea spp., with pectobacterium cypripedii, formed a group distinct from other pathogenic taxa. pectobacterium, brenneria, dickeya, and samsonia formed a contiguous clade. samsonia ... | 2007 | 17451899 |
| biostraticola tofi gen. nov., spec. nov., a novel member of the family enterobacteriaceae. | bacterial strain bf36t, isolated from the biofilm of a tufa deposit in a hard water rivulet, was characterized by a polyphasic taxonomic approach. cells of these organisms were gram-negative, motile, nonpigmented, rod-shaped, non-endospore-forming, and facultatively anaerobic. cells, organized in loose consortia, were coated by a massive slime layer. phylogenetic analyses using 16s rrna gene sequences showed that strain bf36t was a member of the family enterobacteriaceae, class gammaproteobacter ... | 2008 | 18324435 |
| is 16s rdna a reliable phylogenetic marker to characterize relationships below the family level in the enterobacteriaceae? | the phylogenetic relationships of multiple enterobacterial species were reconstructed based on 16s rdna gene sequences to evaluate the robustness of this housekeeping gene in the taxonomic placement of the enteric plant pathogens erwinia, brenneria, pectobacterium, and pantoea. four data sets were compiled, two of which consisted of previously published data. the data sets were designed in order to evaluate how 16s rdna gene phylogenies are affected by the use of different plant pathogen accessi ... | 2008 | 18504519 |
| identification of quorum sensing signal molecules and oligolignols associated with watermark disease in willow (salix sp.). | the bacterium brenneria salicis is the causal agent of watermark disease in willow. this work shows the importance of in situ studies and high-resolution separation of biological samples with ultrahigh performance liquid chromatography combined with ion trap mass spectrometry to unambiguously identify molecular compounds associated with this disease. approximately 40 oligolignols accumulated in wood sap of watermark diseased willow, and are indicative for degradation of the xylem cell wall, of w ... | 2008 | 18675599 |
| host range and molecular phylogenies of the soft rot enterobacterial genera pectobacterium and dickeya. | abstract pectobacterium and dickeya spp. are related broad-host-range entero-bacterial pathogens of angiosperms. a review of the literature shows that these genera each cause disease in species from at least 35% of angiosperm plant orders. the known host ranges of these pathogens partially overlap and, together, these two genera are pathogens of species from 50% of angiosperm plant orders. notably, there are no reported hosts for either genus in the eudicots clade and no reported dickeya hosts i ... | 2007 | 18944180 |
| isolation and characterization of brenneria quercina, causal agent for bark canker and drippy nut of quercus spp. in spain. | abstract the drippy nut disease of oak was first described in california in 1967 and, since then, the causal agent has not been reported in any other area. this study describes for the first time in europe the isolation of brenneria (erwinia) quercina from bark canker in addition to drippy bud and drippy nut in quercus ilex and q. pyrenaica. the bark canker and drippy bud symptoms were not previously described as caused by this bacterium. no fungal pathogens were associated with any of the sympt ... | 2003 | 18944364 |
| occurrence of shallow bark canker of walnut (juglans regia) in southern provinces of iran. | from april 2001 to november 2002, samples of walnut branches and trunks with symptoms of shallow bark canker were collected from fars and kohgiluyeh-va-boyerahmad provinces. symptoms of the disease were small cracks in the bark of the trunk and scaffold branches of mature trees with dark watery exudates which stained the affected trunk or limb. by removal of phelloderm, extensive necrosis of the underlying tissues was observed. in some cases, necrosis extended to cambium and outer xylem. sixty-o ... | 2007 | 19069966 |
| genetic loci involved in rubrifacine production in the walnut pathogen brenneria rubrifaciens. | brenneria rubrifaciens produces a unique red pigment known as rubrifacine that has been hypothesized to play a role in pathogenesis on walnut. analysis of dna flanking the tn5 insertion site in 20 rubrifacine minus (pig(-)) mutants identified three regions required for rubrifacine production. the first region was homologous to nonribosomal peptide synthetases (nrps), the second was homologous to autoinducer synthase genes (expi homologs), and the third region was homologous to the slya gene of c ... | 2009 | 19159306 |
| diverse antibacterial activity of pectobacterium carotovorum subsp.carotovorum isolated in korea. | fifty-four pectobacterium carotovorum subsp. carotovorum strains isolated in korea were characterized by a spectrum of antibacterial activities against 7 indicator strains chosen to represent various regions and host plants. all p. carotovorum subsp. carotovorum isolates tested could be grouped into 4 classes depending on the pattern of antibacterial substance production. all tested strains had dna fragment(s) homologous to the genes encoding carotovoricin and 21 of them had genes homologous to ... | 2009 | 19190407 |
| antimicrobial activity of some macrophytes from lake manzalah (egypt). | the antimicrobial activities of aqueous and organic solvents (chloroform, ethanol and methanol) extracts of four plants ceratophyllum demersum l., eichhornia crassipes, potamogeton crispus and potamogeton pectinatus were tested in vitro against seventeen different microorganisms including gram-positive and gram-negative bacteria and fungi. nine of these identified organisms were obtained from different sources, bacillus subtilis 1020, bacillus cereus 1080, staphylococcus aureus, erwinia carotovo ... | 2008 | 19205264 |
| willow wood sap promotes the density-dependent pathogenesis of brenneria salicis. | brenneria salicis resides in symptomless willow (salix spp.) and other tree species, but only willow trees develop watermark disease. to understand the conversion of b. salicis into a pathogen, its pathogenicity and differential growth in the various tree species are studied. brenneria salicis was detected by plating and polymerase chain reaction-based techniques. cell wall degradation and quorum sensing (qs) were assayed as possible pathogenicity mechanisms in wood. differences in b. salicis gr ... | 2009 | 19220403 |
| brenneria salicis, the bacterium causing watermark disease in willow, resides as an endophyte in wood. | brenneria salicis has been studied in willow wood only in relation to watermark disease. in this pathogenic condition, the bacterium occurs at high concentrations. pathogenicity of b. salicis is still uncontrollable and the disease unpredictable because the plant-bacteria interaction is not understood. thanks to molecular techniques b. salicis can be detected at low concentrations, which are found in most non-pathogenic interactions. brenneria salicis was identified and traced with a new specifi ... | 2009 | 19220404 |
| phylogenetic evidence for extensive horizontal gene transfer of type iii secretion system genes among enterobacterial plant pathogens. | this study uses sequences from four genes, which are involved in the formation of the type iii secretion apparatus, to determine the role of horizontal gene transfer in the evolution of virulence genes for the enterobacterial plant pathogens. sequences of erwinia, brenneria, pectobacterium, dickeya and pantoea were compared (a) with one another, (b) with sequences of enterobacterial animal pathogens, and (c) with sequences of plant pathogenic gamma and beta proteobacteria, to evaluate probable p ... | 2009 | 19643761 |
| origin and examination of a leafhopper facultative endosymbiont. | eukaryotes engage in intimate interactions with microbes that range in age and type of association. although many conspicuous examples of ancient insect associates are studied (e.g., buchnera aphidicola), fewer examples of younger associations are known. here, we further characterize a recently evolved bacterial endosymbiont of the leafhopper euscelidius variegatus (hemiptera, cicadellidae), called bev. we found that bev, continuously maintained in e. variegatus hosts at uc berkeley since 1984, ... | 2011 | 21336565 |
| Proposal to reclassify Brenneria quercina (Hildebrand & Schroth 1967) Hauben et al. 1999 into a novel genus, Lonsdalea gen. nov., as Lonsdalea quercina comb. nov., descriptions of Lonsdalea quercina subsp. quercina comb. nov., Lonsdalea quercina subsp. iberica subsp. nov. and Lonsdalea quercina subsp. britannica subsp. nov., emendation of the description of the genus Brenneria, reclassification of Dickeya dieffenbachiae as Dickeya dadantii subsp. dieffenbachiae comb. nov., and emendation of the description of Dickeya dadantii. | Bacterial isolates from oak in Spain and Britain, showing symptoms of bark canker and Acute Oak Decline (AOD) respectively, were examined by a polyphasic approach. Both 16S rRNA gene sequencing and multilocus sequence analysis (MLSA), based on partial sequences of gyrB, rpoB, infB and atpD, revealed that the isolates were separated into two genetic groups according to their origin. Their closest phylogenetic relative was B. quercina, the causal agent of drippy nut disease of oak, which clustered ... | 2011 | 21890733 |
| brenneria goodwinii sp. nov., a novel species associated with acute oak decline in britain. | a group of nine gram-negative staining, facultatively anaerobic bacterial strains isolated from native oak trees displaying symptoms of acute oak decline (aod) in britain were investigated using a polyphasic approach. 16s rrna gene sequencing and phylogenetic analysis revealed that these isolates form a distinct lineage within the genus brenneria, family enterobacteriaceae, and are most closely related to brenneria rubrifaciens (97.6 % sequence similarity). mlsa based on four housekeeping genes ... | 2011 | 22140177 |
| massive production of butanediol during plant infection by phytopathogenic bacteria of the genera dickeya and pectobacterium. | plant pathogenic bacteria of the genera dickeya and pectobacterium are broad-host-range necrotrophs which cause soft-rot diseases in important crops. a metabolomic analysis, based on (13)c-nmr spectroscopy, was used to characterize the plant-bacteria interaction. metabolic profiles revealed a decline in plant sugars and amino acids during infection and the concomitant appearance of a compound identified as 2,3-butanediol. butanediol is the major metabolite found in macerated tissues of various h ... | 2011 | 22032684 |