| effects of serpentine soil factors on virginia pine (pinus virginiana) seedlings. | effects of simulated serpentine soil conditions (elevated mg:ca ratio and ni concentration) on seedlings from populations of virginia pine (pinus virginiana mill.) from serpentine and non-serpentine sites were evaluated in sand culture. we determined (1) how seedlings are affected by elevated mg:ca ratio and ni concentrations, (2) if there are interactive effects between mg:ca ratio and ni concentrations on seedling growth, needle pigment concentrations, and nutrition, and (3) if virginia pine p ... | 2000 | 11269965 |
| seedling insensitivity to ozone for three conifer species native to great smoky mountains national park. | field symptoms typical of ozone injury have been observed on several conifer species in great smoky mountains national park, and tropospheric ozone levels in the park can be high, suggesting that ozone may be causing growth impairment of these plants. the objective of this research was to test the ozone sensitivity of selected conifer species under controlled exposure conditions. seedlings of three species of conifers, table mountain pine (pinus pungens), virginia pine (pinus virginiana), and ea ... | 2000 | 15092944 |
| diurnal changes in water conduction in loblolly pine (pinus taeda) and virginia pine (p. virginiana) during soil dehydration. | we studied diurnal changes in water conduction during soil dehydration in 37-month-old seedlings of one virginia pine (pinus virginiana mill.) and two loblolly pine (p. taeda l.) sources, one from north carolina (nc) and the other from the "lost pines" areas of texas (tx), in an environmentally controlled growth chamber. for seedlings of similar biomass, the tx source had higher values of transpiration, needle conductance, and plant hydraulic conductivity under well-watered conditions than the n ... | 1999 | 12651531 |
| retention and loss of cesium-137 by components of the groundcover in a pine (pinus virginiana l.) stand. | | 1967 | 6048951 |
| suitability of some southern and western pines as hosts for the pine shoot beetle, tomicus piniperda (coleoptera: scolytidae). | the pine shoot beetle, tomicus piniperda (l.), is an exotic pest that has become established in north america. discovered in ohio in 1992, it has since been found in at least 13 states and parts of canada. the beetle can cause significant growth loss in pines, and it represents a potential threat to trees in areas where it has not yet become established. to evaluate this threat to native pines, field and laboratory tests were conducted on several common and important southern and western species ... | 2004 | 15154468 |
| evaluation of biological and biorational control tactics for suppression of nantucket pine tip moth damage in virginia pine christmas trees. | a study was performed to evaluate the potential of the egg parasitoid trichogramma exiguum pinto and platner (hymenoptera: trichogrammatidae), the newly registered insect growth regulator (igr) tebufenozide (confirm), and a modified spray technique (top whorl only pesticide application) for suppression of nantucket pine tip moth, rhyacionia frustrana (comstock) (lepidoptera: tortricidae), damage in virginia pine, pinus virginiana mill., christmas trees. augmentative releases of t. exiguum failed ... | 2005 | 15889732 |
| antioxidants enhance in vitro plant regeneration by inhibiting the accumulation of peroxidase in virginia pine (pinus virginiana mill.). | plant tissue necrosis and subsequent cell death are usually observed during in vitro regeneration in conifers, especially in plant regeneration via somatic organogenesis in pine species. cell death is correlated with the elevated levels of peroxides. in this investigation, the effects of antioxidants on in vitro regeneration of virginia pine (pinus virginiana mill.) were evaluated. antioxidants, polyvinylpolypyrrolidone (pvpp) and 1,4-dithio-dl-threitol (dtt), were found to improve callus format ... | 2004 | 15042408 |
| high efficiency inducible gene expression system based on activation of a chimeric transcription factor in transgenic pine. | inducible gene expression systems are needed in functional genomics of tree species. a glucocorticoid-inducible gene expression system was established in a gymnosperm species virginia pine (pinus virginiana mill.) through agrobacterium tumefaciens-mediated genetic transformation. the results demonstrate that expression of the m-gfp5-er reporter gene was tightly controlled and 0.1 microm of the glucocorticoid hormone triamcinolone was able to induce m-gfp5-er expression in transgenic cells. diffe ... | 2005 | 16133346 |
| regulated gene expression by glucocorticoids in cultured virginia pine (pinus virginiana mill.) cells. | the effects of six glucocorticoids (dexamethasone, hydrocortisone, 6-methylprednisolone, prednisolone, prednisone, and triamcinolone) on inducible gene expression, based on the chimaeric transcriptional activator gvg and carried by the binary expression vector pindex3-m-gfp5-er, were evaluated in transgenic virginia pine cell cultures. the concentration that activated gvg transcription factor activity, the level of inducible m-gfp5-er expression, and the kinetics of inducible m-gfp5-er expressio ... | 2004 | 15181111 |
| post-transcriptional gene silencing induced by short interfering rnas in cultured transgenic plant cells. | short interfering rna (sirna) is widely used for studying post-transcriptional gene silencing and holds great promise as a tool for both identifying function of novel genes and validating drug targets. two sirna fragments (sirna-a and -b), which were designed against different specific areas of coding region of the same target green fluorescent protein (gfp) gene, were used to silence gfp expression in cultured gfp transgenic cells of rice (oryza sativa l.; os), cotton (gossypium hirsutum l.; gh ... | 2004 | 15629049 |
| differential gene silencing induced by short interfering rna in cultured pine cells associates with the cell cycle phase. | the double-stranded short interfering rna (sirna) molecules can silence targeted genes through sequence-specific cleavage of the cognate rna transcript. the rapid adoption of technologies based on this sirna interference mechanism has been a widely used method to analyze gene function in plants, invertebrates, and mammalian systems. in order to understand the dynamics of sirna-mediated gene inactivation during cell division, we have investigated the relationship between the cell cycle phase and ... | 2006 | 16341704 |
| inducible antisense-mediated post-transcriptional gene silencing in transgenic pine cells using green fluorescent protein as a visual marker. | an inducible post-transcriptional gene silencing (ptgs) system was established in virginia pine (pinus virginiana mill.) cells. this system is based on the activation of an antisense gfp gene construct by a chimeric transcriptional activator gvg (gal4-binding domain-vp16 activation domain-glucocorticoid receptor fusion) upon application of the inducer to gfp transgenic cell lines. a detailed characterization of the inducible ptgs system in transgenic cell lines demonstrated that this system is s ... | 2005 | 15919671 |
| polyamines promote root elongation and growth by increasing root cell division in regenerated virginia pine (pinus virginiana mill.) plantlets. | polyamines have been demonstrated to play an important role in adventitious root formation and development in plants. here, we present a detailed analysis of influence of exogenously added polyamines on adventitious root development and its relationship to cold tolerance in virginia pine (pinus virginia mill.). our results demonstrated that polyamines putrescine (put), spermidine (spd), and spermine (spm) at 0.001 mm improve rooting frequency and promote root elongation. put, spd, and spm at 0.0 ... | 2005 | 16160835 |
| expression of a transcription factor from capsicum annuum in pine calli counteracts the inhibitory effects of salt stress on adventitious shoot formation. | transcription factors regulating the stress-responsive gene expression play an important role in plant stress adaptation. in this study, we examined the salt stress tolerance of transgenic virginia pine (pinus virginiana mill.) overexpressing a capsicum annuum erf/ap2-type transcription factor (capf1), which may enhance the ability of transgenic plants to tolerate various kinds of stresses during vegetative growth. capf1 transgene increased the salt and oxidative stress tolerances of pine tissue ... | 2006 | 16767459 |
| overexpression of the pepper transcription factor capf1 in transgenic virginia pine (pinus virginiana mill.) confers multiple stress tolerance and enhances organ growth. | transcription factors play an important role in regulating gene expression in response to stress and pathogen tolerance. we describe here that overexpression of an erf/ap2 pepper transcription factor (capf1) in transgenic virginia pine (pinus virginiana mill.) confers tolerance to heavy metals cadmium, copper, and zinc, to heat, and to pathogens bacillus thuringiensis and staphylococcus epidermidis, as by the survival rate of transgenic plants and the number of decreasing pathogen cells in trans ... | 2005 | 16244910 |
| craterellus fallax, a black trumpet mushroom from eastern north america with a broad host range. | phylogenetic analysis of its sequences of members of the craterellus cornucopioides complex (black trumpet mushrooms) supports the taxonomic separation of craterellus fallax apart from c. cornucopioides, with which it has been synonymized in the past. examination of pinus virginiana ectomycorrhizal (ecm) root tips and sequence comparison with other insufficiently identified environmental sequences from roots of tsuga, quercus, and possibly castanea supports a broad host range in north america fo ... | 2010 | 20602121 |
| hydraulic patterns and safety margins, from stem to stomata, in three eastern u.s. tree species. | adequate water transport is necessary to prevent stomatal closure and allow for photosynthesis. dysfunction in the water transport pathway can result in stomatal closure, and can be deleterious to overall plant health and survival. although much is known about small branch hydraulics, little is known about the coordination of leaf and stem hydraulic function. additionally, the daily variations in leaf hydraulic conductance (k(leaf)), stomatal conductance and water potential (+¿(l)) have only bee ... | 2011 | 21724585 |
| extended host range of agrobacterium tumefaciens in the genus pinus. | two-to 4-month-old seedlings of nine pine species (pinus eldarica medw., pinus elliottii engelm., pinus jeffreyi grev. & balf., pinus lambertiana dougl., pinus ponderosa laws., pinus radiata d. don, pinus sylvestris l., pinus taeda l., pinus virginiana mill), douglas fir (pseudotsuaa menziesii (mirb.) franco) and incense cedar (libocedrus decurrens torr.) were inoculated with five strains of agrobacterium tumefaciens. transformation occurred in all conifer species tested as determined by gall fo ... | 1990 | 16667394 |
| spatial patterns of ectomycorrhizal fungal inoculum in arbuscular mycorrhizal barrens communities: implications for controlling invasion by pinus virginiana. | invasion of globally threatened ecosystems dominated by arbuscular mycorrhizal plants, such as the alkaline prairies and serpentine barrens of eastern north america, by species of ectomycorrhizal (ecm) pine (pinus) seriously threatens the persistence, conservation, and ongoing restoration of these rare plant communities. using maryland serpentine barrens and an ohio alkaline prairie complex as model systems, we tested the hypothesis that the invasiveness of virginia pine (pinus virginiana l.) in ... | 2007 | 17356853 |
| responses of seeds of pinus virginiana to light. | | 1961 | 16655506 |
| allozyme diversity in pinus virginiana (pinaceae): intraspecific and interspecific comparisons. | two of the four members of subsection contortae of the genus pinus occur in the southeastern united states: pinus virginiana, which ranges throughout the southern and central appalachian mountains, and p. clausa, which is restricted to florida and southern alabama. we examined allozyme variation within p. virginiana and genetic relationships between this species and the two varieties of p. clausa (var. clausa and var. immuginata). p. virginiana maintains more genetic diversity at both the specie ... | 1997 | 21708545 |