| composition of gum turpentines of pines. xiv. a report on three mexican pines: pinus ayacahuite, pinus cembroides, and pinus pinceana. | | 1952 | 13022428 |
| mycorrhizal synthesis of lactarius indigo (schw.) fr. with five neotropical pine species. | this paper describes for the first time the ectomycorrhiza synthesized between two guatemalan strains of lactarius indigo (schw.) fr. and the neotropical species pinus ayacahuite var. ayacahuite ehren, p. hartwegii lindl., p. oocarpa schiede ex schltdl. var oocarpa, p. pseudostrobus lindl. and p. rudis endl. the synthesis was carried out in a controlled growth chamber using plastic containers with peat moss-vermiculite substrate and mycelial inoculum. mycorrhiza were obtained 25 days after inocu ... | 2005 | 16133250 |
| in vitro regeneration of plantlets from mature embryos of pinus ayacahuite. | a plantlet regeneration protocol was developed for pinus ayacahuite var. ayacahuite (ehrenb.). embryos from mature seeds from ten provenances were cultured in a 16-h photoperiod for 3 days on a medium containing 30 mm sucrose and 0.7% agar. cotyledons from these embryos were subcultured onto mcm medium (bornman 1983) supplemented with 50 micro m n(6)-benzyladenine and 90 mm sucrose for 2 weeks. bud development and shoot elongation were maximized by subculturing the explants on half strength ae m ... | 1997 | 14759888 |
| root induction in pinus ayacahuite by co-culture with agrobacterium tumefaciens strains. | transformation of in-vitro-derived shoots of pinus ayacahuite ehrenb. was achieved by co-culture with an oncogenic strain (a281 x 200) of agrobacterium tumefaciens. during co-culture rooting also occurred; however, this rooting was not induced by genetic transformation of host cells, because a "disarmed" strain of a. tumefaciens (eha101) also induced rooting. furthermore, direct contact between shoots and bacterial cells was not required. rooting occurred in agar-solidified medium and in a soill ... | 1999 | 12651560 |