| comparison of daptomycin, vancomycin, and ampicillin-gentamicin for treatment of experimental endocarditis caused by penicillin-resistant enterococci. | infections with enterococci that are resistant to multiple antibiotics are an emerging clinical problem. we evaluated the antibiotic treatment of experimental enterococcal endocarditis caused by two strains with different mechanisms of penicillin resistance. enterococcus faecalis hh-22 is resistant to aminoglycosides and penicillin on the basis of plasmid-mediated modifying enzymes; enterococcus raffinosus sf-195 is susceptible to aminoglycosides but is resistant to penicillin on the basis of lo ... | 1992 | 1329632 |
| ampicillin-resistant enterococcus raffinosus in an acute-care hospital: case-control study and antimicrobial susceptibilities. | a prospective study identified 9 (32%) of 28 ampicillin-resistant (mic greater than or equal to 16 micrograms/ml) enterococcus isolates as enterococcus raffinosus. a case-control study found no significant differences with respect to underlying diseases, catheterization, or surgery between patients with ampicillin-resistant e. raffinosus and those with ampicillin-susceptible enterococcus spp. prior treatment with antibiotics and prolonged hospitalization were more frequent among patients with am ... | 1991 | 1774284 |
| comparison of enterococcus raffinosus with enterococcus avium on the basis of penicillin susceptibility, penicillin-binding protein analysis, and high-level aminoglycoside resistance. | we reidentified our laboratories' collections of 57 enterococcal isolates previously classified as enterococcus avium by the api rapid strep identification system (analytab products, plainview, n.y.) with the identification criteria recommended by facklam and collins (r. r. facklam and m. d. collins, j. clin. microbiol. 27: 731-734, 1989). thirty isolates were identified as true e. avium, 25 isolates were identified as e. raffinosus, and 2 isolates were identified as e. pseudoavium. e. raffinosu ... | 1991 | 1929301 |
| species identities of enterococci isolated from clinical specimens. | conventional tests and commercially available systems were used to determine the species identities of clinical isolates of enterococci. strict adherence to the conventional test scheme of facklam and collins (r. r. facklam and m. d. collins, j. clin. microbiol. 27:731-734, 1989) resulted in the misidentification of lactose-negative enterococcus faecalis isolates as enterococcus solitarius, but this problem was overcome by the application of additional tests. the commercially available systems t ... | 1990 | 2108992 |
| enterococcus raffinosus sp. nov., enterococcus solitarius sp. nov. and enterococcus pseudoavium sp. nov. | deoxyribonucleic acid base composition, deoxyribonucleic acid-deoxyribonucleic acid hybridization, and biochemical studies were performed on some enterococci from clinical sources of uncertain taxonomic position. our results indicate that 6 human strains, a single clinical isolate and a strain from bovine mastitis are genetically distinct from each other and all other previously described enterococcus species and constitute three new species, for which the names enterococcus raffinosus, enteroco ... | 1989 | 2721919 |
| penicillin resistance and aminoglycoside-penicillin synergy in enterococci. | susceptibility to penicillin, vancomycin, imipenem, streptomycin, kanamycin and gentamicin was tested in 130 clinical isolates of enterococcus spp. by an agar dilution method. penicillin resistance (mic > 8 mg/l) was only observed among strains of enterococcus faecium and enterococcus raffinosus. thirty-nine percent of the penicillin-resistant enterococci showed low-level resistance to at least one of the three aminoglycosides tested (gentamicin, kanamycin and streptomycin). six enterococcus str ... | 1995 | 7656661 |
| dna hybridization and contour-clamped homogeneous electric field electrophoresis for identification of enterococci to the species level. | in this study, 113 enterococcus faecium, 37 enterococcus faecalis, 24 enterococcus gallinarum, 15 enterococcus raffinosus, and 13 enterococcus casseliflavus clinical isolates and american type culture collection (atcc) strains were evaluated by contour-clamped homogeneous electric field electrophoresis. thirty-one of the e. faecium, 22 of the e. faecalis, 24 of the e. gallinarum, 15 of the e. raffinosus, and 13 of the e. casseliflavus isolates were also evaluated by dna-dna hybridization. genomi ... | 1995 | 7699030 |
| resistance of enterococcus faecium to neutrophil-mediated phagocytosis. | during a previous study of the opsonic requirements for neutrophil (polymorphonuclear leukocyte [pmn])-mediated killing of enterococci, we identified two strains of enterococcus faecium (tx0015 and tx0016) that were resistant to pmn-mediated killing. to better define the mechanism of this resistance, we examined phagocytosis with a fluorescence assay and found that tx0016 was completely resistant to phagocytosis by pmns; this finding was confirmed by electron microscopy. examination of multiple ... | 1994 | 7960141 |
| infections by ampicillin-resistant enterococci: a case-control study. | we identified 17 (20%) of 83 consecutive enterococcal isolates from hospitalized patients with documented infection as high-level ampicillin-resistant enterococci (are). of these, 16 isolates were identified as enterococcus faecium and 1 isolate as enterococcus raffinosus. a case-control study found no significant differences with respect to underlying diseases, central venous catheterization, nosocomial acquisition of the infection and sites of infection. patients with are infection were older ... | 1994 | 8077987 |
| in vitro activity of sparfloxacin and clinafloxacin against multidrug-resistant enterococci. | we evaluated the in vitro susceptibility of 140 clinical enterococcal isolates to the quinolones sparfloxacin and clinafloxacin. isolates included enterococcus faecalis (107), enterococcus faecium (29), enterococcus raffinosus (3), and one enterococcus gallinarum. there were 111 isolates that showed high-level [minimum inhibitory concentrations (mics) > or = 2000 micrograms/ml] resistance to gentamicin and were resistant to high levels of all other aminoglycosides; five isolates produced beta-la ... | 1993 | 8243036 |
| evaluation of vitek and api 20s for species identification of enterococci. | the performance of the vitek system and the api 20s for identifying enterococci to the species level was evaluated in comparison with a conventional reference test scheme. a total of 369 isolates originally derived from a sample from 97 medical centers were tested, and the percentage of correctly identified organisms was (vitek/api): 97.6%/79.2%, 95.3%/91.2%, and 86.0%/20.7% for enterococcus faecalis, enterococcus faecium, and enterococcus non-faecalis-non-faecium species, respectively. in addit ... | 1995 | 8582135 |
| plasmid-borne high-level resistance to gentamicin in enterococcus hirae, enterococcus avium, and enterococcus raffinosus. | enterococcus hirae, e. avium, and e. raffinosus isolated in romania, tunisia, and portugal harbored plasmids picc8, pip1700, and pip1701, respectively, encoding resistance to high levels of gentamicin (gmr). the gmr marker was carried on pip1700 by a tn4001-like element and on picc8 and pip1701 by tn4001-truncated structures. picc8 carried, in addition to gmr, chloramphenicol, erythromycin, and tetracycline-minocycline (tetm) resistance determinants. the gene tetm of picc8 was carried on a tn916 ... | 1996 | 8723479 |
| species identification of enterococci via intergenic ribosomal pcr. | accurate species identification of enterococci has become important with the wide prevalence of acquired vancomycin resistance and the presence of less epidemiologically important, inherently vancomycin-resistant enterococci. using a collection of enterococcal strains, we found that pcr amplification of the intergenic spacer (its-pcr) between the 16s and 23s rrna genes can produce amplicon profiles characteristic of the enterococcus examined. the species examined were group i enterococci (entero ... | 1997 | 9114380 |
| vancomycin-resistant enterococcus raffinosus: molecular epidemiology, species identification error, and frequency of occurrence in a national resistance surveillance program. | enterococcal blood stream infections are the third most common among all nosocomial blood stream infections in the united states and the occurrence of glycopeptide (vancomycin, teicoplanin) resistance in these isolates has markedly increased. control of hospital-acquired infections with vancomycin-resistant enterococci requires high quality antimicrobial susceptibility test methods and species identification procedures as a supplement to epidemiologic investigation and appropriate infection cont ... | 1997 | 9350415 |
| recovery of high-level streptomycin-resistant enterococci from hemodialysis water and dialysate in 85 greek renal units. | in the 85 renal units of greece, enterococci were recovered from 10 samples of tap water, 6 of treated hemodialysis water, and 21 of dialysate. eleven isolates were enterococcus faecium, and 8 were enterococcus raffinosus; 6 other additional enterococcal species were found. twenty-two strains exhibited high-level resistance to streptomycin, 16 were resistant to rifampicin, and one to erythromycin. in our hemodialysis units, treated water and dialysate raise concern regarding transfer to patients ... | 1999 | 10530647 |
| sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci. | simple pcr and sequencing assays that utilize a single pair of degenerate primers were used to characterize a 438-bp-long dna fragment internal (soda(int)) to the soda gene encoding the manganese-dependent superoxide dismutase in 19 enterococcal type strains (enterococcus avium, enterococcus casseliflavus, enterococcus cecorum, enterococcus columbae, enterococcus dispar, enterococcus durans, enterococcus faecalis, enterococcus faecium, enterococcus flavescens, enterococcus gallinarum, enterococc ... | 2000 | 10618129 |
| evaluation of a new system, vitek 2, for identification and antimicrobial susceptibility testing of enterococci. | we evaluated the new automated vitek 2 system (biomérieux) for the identification and antimicrobial susceptibility testing of enterococci. the results obtained with the vitek 2 system were compared to those obtained by reference methods: standard identification by the scheme of facklam and sahm [r. r. facklam and d. f. sahm, p. 308-314, in p. r. murray et al., ed., manual of clinical microbiology, 6th ed., 1995] and with the api 20 strep system and, for antimicrobial susceptibility testing, brot ... | 2000 | 10834961 |
| susceptibility to quinupristin/dalfopristin and other antibiotics of vancomycin-resistant enterococci from the uk, 1997 to mid-1999. | susceptibility to quinupristin/dalfopristin and other antibiotics was studied for clinical isolates of vancomycin-resistant enterococci (vre) referred by uk hospitals between january 1997 and june 1999. single isolates of vre from 858 patients in 136 hospitals were received, of which 76% were enterococcus faecium and 21% were enterococcus faecalis, the remainder comprising minor species. most isolates were multi-resistant. after allowing for the effect of blood, which raised the mics of quinupri ... | 2000 | 10882701 |
| identification of enterococcus species and phenotypically similar lactococcus and vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences. | data from four recent studies (s. h. goh et al., j. clin. microbiol. 36:2164-2166, 1998; s. h. goh et al., j. clin. microbiol. 34:818-823, 1996; s. h. goh et al., j. clin. microbiol. 35:3116-3121, 1997; a. y. c. kwok et al., int. j. syst. bacteriol. 49:1181-1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (cpn60) gene, amplified by pcr with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (id). this cpn60 ... | 2000 | 11060051 |
| diversity of domain v of 23s rrna gene sequence in different enterococcus species. | the highly conserved central loop of domain v of 23s rna (nucleotides 2042 to 2628; escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin b antibiotics. dna encoding domain v (590 bp) of several species of enterococcus was amplified by pcr. twenty enterococcal isolates were tested, including enterococcus faecium (six isolates), enterococcus faecalis, enterococcus avium, enterococcus durans, e ... | 2000 | 11060057 |
| application of trna intergenic spacer pcr for identification of enterococcus species. | trna intergenic spacer pcr (tdna-pcr) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. this technique was carried out for 124 strains belonging to 17 enterococcal species and generated dna fragments, which were separated by capillary electrophoresis. tdna-pcr enabled us to discriminate for all species tested. enterococcus faecium showed minor but reproducible differences with enterococcus durans, while enterococcus hirae was easily disti ... | 2000 | 11060090 |
| evidence for horizontal gene transfer in evolution of elongation factor tu in enterococci. | the elongation factor tu, encoded by tuf genes, is a gtp binding protein that plays a central role in protein synthesis. one to three tuf genes per genome are present, depending on the bacterial species. most low-g+c-content gram-positive bacteria carry only one tuf gene. we have designed degenerate pcr primers derived from consensus sequences of the tuf gene to amplify partial tuf sequences from 17 enterococcal species and other phylogenetically related species. the amplified dna fragments were ... | 2000 | 11092850 |
| vertebral osteomyelitis caused by enterococcus raffinosus. | enterococcus raffinosus is a rare isolate in clinical specimens. a case of vertebral osteomyelitis caused by e. raffinosus in an elderly patient is described and confirms this organism to be an opportunistic human pathogen. | 2001 | 11283118 |
| identification of enterococcal isolates by temperature gradient gel electrophoresis and partial sequence analysis of pcr-amplified 16s rdna variable v6 regions. | based on partial sequence analysis of pcr-amplified 16s rdna variable v6 regions of 14 enterococcal type strains, enterococcus faecalis, enterococcus mundtii, enterococcus gallinarum, enterococcus avium, enterococcus raffinosus and enterococcus saccharolyticus showed characteristic sequence motifs which made it possible to separate them into six individual species lines. furthermore, two species cluster groups could be identified, including (i) enterococcus faecium, enterococcus durans, enteroco ... | 2001 | 11430498 |
| in vitro assessment of urinary isolates of ampicillin-resistant enterococci. | susceptibility and minimum inhibitory concentration (mic) studies of ampicillin-resistant enterococci (are) were performed with vancomycin, ciprofloxacin, and trovafloxacin. ampicillin mics were determined to make comparisons with achievable urinary concentrations of ampicillin. | 2002 | 11847942 |
| enterococcus gilvus sp. nov. and enterococcus pallens sp. nov. isolated from human clinical specimens. | light yellow-pigmented (strain pq1) and yellow-pigmented (strain pq2), gram-positive, non-spore-forming, nonmotile bacteria consisting of pairs or chains of cocci were isolated from the bile of a patient with cholecystitis (pq1) and the peritoneal dialysate of another patient with peritonitis (pq2). morphologically and biochemically, the organisms phenotypically belonged to the genus eterococcus. whole-cell protein (wcp) analysis and sequence analysis of a segment of the 16s rrna gene suggested ... | 2002 | 11923322 |
| enterococcus hermanniensis sp. nov., from modified-atmosphere-packaged broiler meat and canine tonsils. | isolates 302, 334, 356, 377 and 379, detected in modified-atmosphere-packaged broiler meat, together with strains lmg 12317t and lmg 13617, detected in dog tonsils, were analysed in a polyphasic taxonomy study, including numerical analysis of ribopatterns and whole-cell protein patterns, 16s rrna gene sequence analysis, dna-dna hybridization and determination of some phenotypic properties. the results indicated that these isolates represent a novel species in the genus enterococcus. the isolates ... | 2004 | 15388750 |
| microbiological quality of sous and tamarind, traditional drinks consumed in jordan. | this study was conducted to evaluate the microbiological quality of sous (a drink prepared by extracting dried roots of glycyrrhiza glabra) and tamarind (a drink prepared by infusing tamarindus indica dried pulp), traditional drinks consumed in jordan. twenty-one samples of sous and 44 samples of tamarind were collected from the local market in amman, jordan. water is the major component of the drinks. sous drink is characterized by having an alkaline ph (range, 6.6 to 9.9; mean, 8.6), whereas t ... | 2005 | 15830669 |
| [clinical and epidemiologic analysis of intestinal tract colonization with vancomycin-resistant enterococci in an intensive care unit]. | intestinal tract colonization with vancomycin resistant enterococci (vre) was studied during five months and 25 days. out of 171 patients hospitalized in the intensive care unit, 124 (73%) were included in this study. thirty five of them (28%) were recognized as colonized with vre. vre isolates (n = 35) were identified as enterococcus faecium (n = 18), enterococcus gallinarum (n = 16), and enterococcus raffinosus (n = 1). all of them were resistant to vancomycin (mic90 = 512 microg/ml) and to te ... | 2005 | 15991477 |
| enterococcus raffinosus infection in an immunosuppressed patient: case report and review of the literature. | enterococcus raffinosus rarely occurs in humans. we describe the case of a haematoma infected by e. raffinosus in a 72-year-old patient undergoing immunosuppressive therapy. this study confirms the results of previous reports about the pathogenic potential of this bacterium in humans. | 2004 | 16230189 |
| identification and antagonistic activity of lactic acid bacteria occurring in porcine blood from industrial slaughterhouses--a preliminary study. | ninety-seven lactic acid bacteria (lab) were isolated from slaughterhouse porcine blood in order to select autochthonous lab strains for use as biopreservatives of this by-product. they were identified by 16s rdna sequencing; and their inhibition capacity was determined against four bacterial species frequently found in contaminated blood, i.e. staphylococcus aureus, escherichia coli, pseudomonas fluorescens and bacillus spp. the taxonomic study showed an unexpected low diversity of lab in blood ... | 2006 | 16278029 |
| enterococcus devriesei sp. nov., associated with animal sources. | the taxonomic position of two bovine strains, lmg 13603 and lmg 14595, assigned to the species enterococcus raffinosus on the basis of biochemical features, was reinvestigated. both reference strains and two other isolates, 6/1 (=lmg 22829) originating from a charcoal-broiled river lamprey and ie38.4 (=lmg 22830) from the air of a poultry slaughter by-product processing plant, occupied a clearly separate position, on the basis of sequence analysis of the housekeeping gene phes (encoding the phen ... | 2005 | 16280513 |
| first vand-type vancomycin-resistant enterococcus raffinosus isolate. | | 2006 | 17000737 |
| hospital outbreak of vancomycin-resistant enterococci caused by a single clone of enterococcus raffinosus and several clones of enterococcus faecium. | a mixed outbreak caused by vancomycin-resistant enterococcus raffinosus and enterococcus faecium carrying the vana gene was analysed. the outbreak occurred in a large hospital in poland and affected 27 patients, most of whom were colonised, in three wards, including the haematology unit. the e. raffinosus isolates had a high-level multiresistant phenotype and were initially misidentified as enterococcus avium; their unambiguous identification was provided by multilocus sequence analysis. the mol ... | 2007 | 17617184 |
| multidrug-resistant enterococcus raffinosus from a decubitus ulcer: a case report. | a case of decubitus ulcer infected with multidrug-resistant enterococcus raffinosus is reported in this article. this is the first report of wound infection caused by an enterococcus raffinosus isolate resistant to antibiotics other than beta-lactams, gentamicin, and ciprofloxacin. this case adds to the knowledge base concerning the onset of antibiotic resistance in this uncommon species and confirms the emerging role of daptomycin as a promising alternative in therapy of severe difficult-to-tre ... | 2008 | 18372268 |
| first report of vaginal infection caused by enterococcus raffinosus. | the authors have reported the first case of vaginal infection caused by enterococcus raffinosus. the latter is a rarely identified species, but some of the infections described in the literature should direct some attention to this, often opportunistic pathogen, and its emerging multidrug resistance. | 2008 | 18436605 |
| glycopeptide-resistant enterococcus raffinosus in a haematology unit: an unusual cause of a nosocomial outbreak. | | 2008 | 18783849 |
| endocarditis due to vancomycin-resistant enterococcus raffinosus successfully treated with linezolid: case report and review of literature. | enterococcus raffinosus is scarcely found in clinical samples and even less frequently as etiologic agent of endocarditis. we are herein presenting one case of mitral prosthetic-valve endocarditis in a 77-y-o male due to a vancomycin-resistant enterococcus raffinosus isolate, successfully treated with 6 weeks of linezolid, and a two-year follow up. | 2008 | 19213241 |
| traumatic endophthalmitis caused by enterococcus raffinosus and enterobacter gergoviae. | we report a rare case of polymicrobial traumatic endophthalmitis caused by enterococcus raffinosus and enterobacter gergoviae with successful interventions of complete pars plana vitrectomy with intravitreal injections of vancomycin and amikacin. in addition, the patient achieved a favourable visual acuity of 20/200. | 2009 | 19273651 |
| enterococcus raffinosus endocarditis. first case and literature review. | enterococcus raffinosus, a non-faecalis and non-faecium enterococcus, rarely causes infections in humans. we describe the first reported case of primary bacterial endocarditis caused by e. raffinosus, with a review of the literature. | 2009 | 19359819 |
| comparative analysis on antibiotic resistance characteristics of listeria spp. and enterococcus spp. isolated from laying hens and eggs in conventional and organic keeping systems in bavaria, germany. | by investigating the prevalence and antimicrobial resistance characteristics of gram-positive bacteria from organic and conventional keeping systems of laying hens, it was to be determined to what extent these properties are influenced by the different systems. for this purpose, a total of 799 cloacal swabs and 800 egg samples were examined. prevalences for all selected bacteria from cloacal swabs were much the same for both organic and caged birds: listeria spp.1.3%[org] versus 1.6%[con]; enter ... | 2010 | 19486494 |
| enterococcus raffinosus sinusitis post-aspergillus flavus paranasal infection, in a patient with myelodysplastic syndrome: report of a case and concise review of pertinent literature. | a case of enterococcus raffinosus nosocomial sinusitis which appeared to complicate a previous aspergillus flavus paranasal infection is presented. this uncommon enterococcal species is rarely responsible for human diseases, and has never previously been associated with sinusitis. | 2010 | 20203228 |
| bacteremia caused by non-faecalis and non-faecium enterococcus species at a medical center in taiwan, 2000 to 2008. | summary objectives: human infections due to non-faecalis and non-faecium enterococcus species are emerging but data on the characteristics of these infections are limited. | 2010 | 20433865 |
| host range of enterococcal vana plasmids among gram-positive intestinal bacteria. | the most prevalent type of acquired glycopeptide resistance is encoded by the vana transposon tn1546 located mainly on transferable plasmids in enterococcus faecium. the limited occurrence in other species could be due to the lack of inter-species transferability and/or stability of tn1546-containing plasmids in other species. we investigated the in vitro transferability of 14 pre-characterized vana-containing plasmids hosted by e. faecium (n = 9), enterococcus faecalis (n = 4) and enterococcus ... | 2010 | 21131318 |
| effectiveness of high pressure processing on the hygienic and technological quality of porcine plasma from biopreserved blood. | the effects of a high hydrostatic pressure (hhp) treatment (450mpa, 15min at 20°c) on both the microbiological quality and the functional properties of plasma from biopreserved porcine blood were evaluated. blood was inoculated with enterococcus raffinosus-ps99 (10(7)ufcml(-1)) and stored at 5°c. after 72-h storage, bacterial counts in inoculated samples decreased by 52, 70, 81 and more than 99% for coliforms, pseudomonas spp, hemolytic and proteolytic bacteria, respectively. counts of these bac ... | 2007 | 22064206 |
| preservation of porcine blood quality by means of lactic acid bacteria. | the capacity of 12 lactic acid bacteria (lab) strains to preserve porcine blood during storage was evaluated. a general ability of lab to prevent blood's hemolysis and to maintain the functional properties of plasma was observed. two strains, ps99 (enterococcus raffinosus) and ta43 (lactobacillus reuteri), were selected for studies at 5°c according to their antibacterial activity in blood stored at 15°c. after 144h at 5°c, lower counts of coliforms, pseudomonas spp., proteolytic and hemolytic ba ... | 2006 | 22062312 |
| functional and quality characteristics of the red blood cell fraction from biopreserved porcine blood as influenced by high pressure processing. | the effects of high hydrostatic pressure (hhp) processing, at 400mpa for 15min at 20°c, on the microbiological and functional characteristics of the red blood cell (rbc) fraction obtained from porcine blood, previously preserved by means of lactic acid bacteria (lab) was studied. biopreservation was achieved by incubation of inulin-enriched blood inoculated with a lab strain (enterococcus raffinosus ps99) for 72h at 5°c. results showed that incubation of blood with added e. raffinosus followed b ... | 2008 | 22063343 |
| clinico-epidemiological profile and high-level aminoglycoside resistance in enterococcal septicemia from a tertiary care hospital in east delhi. | emergence of high-level aminoglycoside and glycopeptide resistance has significantly contributed to the mortality, particularly in serious enterococcal infections. | 2011 | 23776782 |
| antibacterial efficacy testing of a bioelectric wound dressing against clinical wound pathogens. | silver-based wound dressings have been developed for the control of bioburden in wounds. however, the popularity and extensive use of silver-based dressings has been associated with emerging microbial resistances to silver. in this study we examined in vitro antibacterial efficacy of a bioelectric dressing containing silver and zinc against various wound pathogens. antibiotic-sensitive clinical wound isolates showed a 100% reduction in bacterial growth, except that enterococcus faecalis isolate ... | 2014 | 24627730 |
| in vitro evaluation of probiotic properties of lactic acid bacteria from the gut of labeo rohita and catla catla. | we report the evaluation of probiotic properties of potent lactic acid bacteria (lab) from the gut of freshwater fishes, labeo rohita and catla catla, for eventually developing probiotic strains for the prevention of bacterial infections in aquaculture and food preservation. five different lab strains were isolated and characterized for their probiotic properties. based on physiological, morphological and biochemical characteristics, three isolates from labeo rohita and two from catla catla were ... | 2015 | 25634754 |
| biologically active antimicrobial and antioxidant substances in the helianthus annuus l. bee pollen. | the objective of this study was to measure the content of flavonoids, polyphenols, and carotenoids in the helianthus annuus l. bee pollen. it was also to evaluate the ability of the dried, frozen, and freeze-dried extracts of sunflower (h. annuus) pollen, its scavenged free radicals and reducing action. another aim of this study was to investigate the antimicrobial in vitro action of the h. annuus pollen extracts against the gram-positive and gram-negative bacteria and fungi. all pollen extracts ... | 2016 | 26674447 |
| antimicrobial resistance and virulence profile of enterococci isolated from poultry and cattle sources in nigeria. | this study investigated the occurrence, antimicrobial resistance and virulence of enterococcus from poultry and cattle farms. three hundred and ninety samples: cloacal/rectal swabs (n = 260) and manure (n = 130] were processed for recovery of enterococcus species. standard bacteriological methods were used to isolate, identify and characterize enterococcus species for antimicrobial susceptibility and expression of virulence traits. detection of antibiotic resistance and virulence genes was carri ... | 2017 | 27987112 |
| bacteriocin production, antibiotic susceptibility and prevalence of haemolytic and gelatinase activity in faecal lactic acid bacteria isolated from healthy ethiopian infants. | the objective of this study was to characterise lactic acid bacteria (lab) isolated from faecal samples of healthy ethiopian infants, with emphasis on bacteriocin production and antibiotic susceptibility. one hundred fifty lab were obtained from 28 healthy ethiopian infants. the isolates belonged to lactobacillus (81/150), enterococcus (54/150) and streptococcus (15/150) genera. lactobacillus species were more abundant in the breast-fed infants while enterococcus dominated the mixed-fed populati ... | 2013 | 23184155 |
| diversity, distribution and antibiotic resistance of enterococcus spp. recovered from tomatoes, leaves, water and soil on u.s. mid-atlantic farms. | antibiotic-resistant enterococci are important opportunistic pathogens and have been recovered from retail tomatoes. however, it is unclear where and how tomatoes are contaminated along the farm-to-fork continuum. specifically, the degree of pre-harvest contamination with enterococci is unknown. we evaluated the prevalence, diversity and antimicrobial susceptibilities of enterococci collected from tomato farms in the mid-atlantic united states. tomatoes, leaves, groundwater, pond water, irrigati ... | 2013 | 24010630 |
| comparison of two chromogenic media for the detection of vancomycin-resistant enterococcal carriage by nursing home residents. | we compared chromid vre and brilliance vre media for the detection of vancomycin-resistant enterococci (vre). using a panel of 28 enterococcal isolates, 10 vana enterococcus faecium and three vana enterococcus faecalis isolates grew as per manufacturers' instructions whilst growth of two vanc and eight vancomycin-susceptible enterococci was inhibited on both media. important differences were noted in the selectivity and chromogenic properties of the two media for vana enterococcus raffinosus and ... | 2016 | 27312688 |
| molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in belgrade, serbia. | in this study the distribution of species and antimicrobial resistance among vancomycin resistant enterococci (vre) recovered from clinical specimens obtained from five hospitals in belgrade was analyzed. strains were further characterized by pulsed-field gel electrophoresis (pfge). polymerase chain reaction (pcr) was used to investigate the presence of vana and vanb genes and pathogenicity factor genes. identification of 194 vre isolates revealed 154 enterococcus faecium, 21 enterococcus faecal ... | 2015 | 26132835 |
| molecular characterization of vancomycin-resistant enterococci in serbia: intensive care unit as the source. | the purpose of this study was to evaluate the molecular relatedness of clinical isolates of vancomycin-resistant enterococci (vre) collected from patients of the clinic for infectious and tropical diseases in belgrade. among 40 isolates available for the investigation, 36 were identified as enterococcus faecium, whereas 2 were enterococcus faecalis and enterococcus raffinosus, respectively. pulsed-field gel electrophoresis (pfge) typing revealed 21 strain types, comprising 7 clusters which conta ... | 2013 | 24292087 |
| vancomycin-resistant enterococci among haemodialysis patients in portugal: prevalence and molecular characterization of resistance, virulence and clonality. | vancomycin-resistant enterococci (vre) among haemodialysis patients has increased rapidly and, to date, there is no report of this incidence in portugal. | 2014 | 24169320 |
| the first clonal spread of vana-positive enterococcus raffinosus in a nursing home. | | 2017 | 28377181 |
| purification and characterization of glutamate decarboxylase from enterococcus raffinosus tccc11660. | glutamate decarboxylase (gad) is the sole enzyme that synthesizes γ-aminobutyric acid through the irreversible decarboxylation of l-glutamate. in this study, the purification and characterization of an unreported gad from a novel strain of enterococcus raffinosus tccc11660 were investigated. the native gad from e. raffinosus tccc11660 was purified 32.3-fold with a recovery rate of 8.3%, using ultrafiltration and ammonium sulfate precipitation, followed by ion-exchange and size-exclusion chromato ... | 2017 | 28101806 |
| first nosocomial outbreak of vana-type vancomycin-resistant enterococcus raffinosus in france. | vancomycin-resistant enterococcus raffinosus has rarely been associated with nosocomial infection and outbreaks. | 2016 | 27726900 |
| draft genome sequence of enterococcus raffinosus strain cftri 2200, isolated from infant fecal material. | the complete genome sequence of enterococcus raffinosus strain cftri 2200, isolated from the fecal material of a 7-month-old infant, is reported. the complete genome consists of 4.237 mbp with a g+c content of 39.47% and 4,242 protein-coding genes, 54 trnas, and 46 rrnas. | 2013 | 24201206 |
| separation and purification of γ-aminobutyric acid from fermentation broth by flocculation and chromatographic methodologies. | to date, the multifunctional γ-aminobutyric acid (gaba) is mainly produced by microbial fermentation in industry. the purpose of this study was to find an effective method for separation and purification of 31.2 g/l initial gaba from the fermentation broth of enterococcus raffinosus tccc11660. to remove the impurities from fermentation broth, flocculation pretreatment using chitosan and sodium alginate was first implemented to facilitate subsequent filtration. ultrafiltration followed two discon ... | 2013 | 23402360 |
| isolation, purification, and characterization of five active diketopiperazine derivatives from endophytic streptomyces suk 25 with antimicrobial and cytotoxic activities. | in our search for new sources of bioactive secondary metabolites from streptomyces sp., the ethyl acetate extracts from endophytic streptomyces suk 25 afforded five active diketopiperazine (dkp) compounds. the aim of this study was to characterize the bioactive compounds isolated from endophytic streptomyces suk 25 and evaluate their bioactivity against multiple drug resistance (mdr) bacteria such as enterococcus raffinosus, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumanii, p ... | 2017 | 28535606 |
| vancomycin-resistant enterococci with vana gene in treated municipal wastewater and their association with human hospital strains. | vancomycin-resistant enterococci (vre) are pathogens of increasing medical importance. in brno, czech republic, we collected 37 samples from the effluent of a wastewater treatment plant (wwtp), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. moreover, we collected 284 gull cloacal swabs from the colony situated 35km downstream the wwtp. samples were cultured selectively. enterococci were identified using maldi-tof ms, phenotypically tested for ... | 2017 | 28763660 |