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characterization of bacteria isolated from wild legumes in the north-western regions of china.nodule isolates from 11 species of wild legumes in north-western china were characterized by numerical taxonomy, pcr-based 16s rrna gene rflp and sequence analyses, dna-dna hybridization, restriction patterns of noddab and nifh genes, and symbiotic properties. based on the results of numerical taxonomy, most of the 35 new isolates were grouped into five clusters (clusters 7, 9, 12, 14 and 15). clusters 7 and 12 were identified as mesorhizobium amorphae and agrobacterium tumefaciens, respectively ...199910555327
mesorhizobium amorphae, a rhizobial species that nodulates amorpha fruticosa, is native to american soils.amorpha fruticosa was inoculated with rhizosphere soil from iowa, usa, and 140 rhizobia isolated from root nodules were compared with mesorhizobium amorphae originating from chinese soils. pcr-rflp patterns of the 16s rrna gene from the isolates and from m. amorphaewere the same. all isolates had a symbiotic plasmid of the same size with a single nifhgene. dna:dna hybridization values, dna g+c content, and induced nod factor patterns also were similar. we concluded that the four genotypes distin ...200212209264
diverse rhizobia associated with woody legumes wisteria sinensis, cercis racemosa and amorpha fruticosa grown in the temperate zone of china.fifty-nine bacterial isolates from root nodules of the woody legumes wisteria sinensis, cercis racemosa and amorpha fruticosa grown in the central and eastern regions of china were characterized with phenotypic analysis, pcr-based 16s and 23s rrna gene rflp, box pcr and 16s rrna gene sequencing. seven main phena were defined in numerical taxonomy, which corresponded to distinct groups within the genera agrobacterium, bradyrhizobium, mesorhizobium and rhizobium in 16s and 23s rrna gene pcr-rflp. ...200516094873
mesorhizobium thiogangeticum sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an indian tropical leguminous plant.the bacterial strain sjt(t), along with 15 other mesophilic, neutrophilic and facultatively sulfur-oxidizing chemolithotrophic isolates, was isolated by enrichment on reduced sulfur compounds as the sole energy and electron source from soils immediately adjacent to the roots of clitoria ternatea, a slender leguminous herb of the lower gangetic plains of india. strain sjt(t) was able to oxidize thiosulfate and elemental sulfur for chemolithoautotrophic growth. 16s rrna and reca gene sequence-base ...200616403872
ameba-associated microorganisms and diagnosis of nosocomial pneumonia.to elucidate the role of ameba-associated microorganisms (aams) as etiologic agents of pneumonia, we screened for legionella spp., parachlamydia acanthamoeba, afipia sp., bosea spp., bradyrhizobium spp., mesorhizobium amorphae, rasbo bacterium, azorhizobium caulinodans, acanthamoeba polyphaga mimivirus, and conventional microorganisms in 210 pneumonia patients in intensive-care units by using culture, polymerase chain reaction, and serologic testing. these resulted in 59 diagnoses in 40 patients ...200616494750
strains of mesorhizobium amorphae and mesorhizobium tianshanense, carrying symbiotic genes of common chickpea endosymbiotic species, constitute a novel biovar (ciceri) capable of nodulating cicer arietinum.to identify several strains of mesorhizobium amorphae and mesorhizobium tianshanense nodulating cicer arietinum in spain and portugal, and to study the symbiotic genes carried by these strains.200717397480
diversity and geographical distribution of rhizobia associated with lespedeza spp. in temperate and subtropical regions of china.eighty-eight root-nodule isolates from lespedeza spp. grown in temperate and subtropical regions of china were characterized by a polyphasic approach. nine clusters were defined in numerical taxonomy and sds-page analysis of whole cell proteins. based upon further characterizations of amplified 16s rdna restriction analysis (ardra), pcr-based restriction fragment length polymorphism of ribosomal igs, 16s rdna sequence analysis and dna-dna hybridization, these isolates were identified as bradyrhi ...200717530227
different mesorhizobium species associated with caragana carry similar symbiotic genes and have common host ranges.fourteen strains representing 11 caragana-nodulating mesorhizobium genomic species were identified as representing mesorhizobium amorphae, mesorhizobium huakuii, mesorhizobium septentrionale and groups related to mesorhizobium plurifarium, mesorhizobium temperatum, mesorhizobium tianshanense and mesorhizobium mediterraneum by sequencing of the 16s rrna gene, 16s-23s internal transcribed spacer, partial housekeeping reca gene, and previously performed sodium dodecyl sulfate polyacrylamide gel ele ...200818422620
genetic diversity and host range of rhizobia nodulating lotus tenuis in typical soils of the salado river basin (argentina).a total of 103 root nodule isolates were used to estimate the diversity of bacteria nodulating lotus tenuis in typical soils of the salado river basin. a high level of genetic diversity was revealed by repetitive extragenic palindromic pcr, and 77 isolates with unique genomic fingerprints were further differentiated into two clusters, clusters a and b, after 16s rrna restriction fragment length polymorphism analysis. cluster a strains appeared to be related to the genus mesorhizobium, whereas cl ...200919074602
invasive robinia pseudoacacia in china is nodulated by mesorhizobium and sinorhizobium species that share similar nodulation genes with native american symbionts.the aim of this work is to describe the diversity of potentially symbiotic bacteria associated with the invasive introduced legume robinia pseudoacacia in china. thirty-three isolates from 33 separate trees and nodules were characterized using restriction length fragment polymorphism and sequencing of 16s rrna, noda, nodc and nifh genes. their 16s rrna gene patterns and sequences placed them in three clades: 85% of isolates were related to the mesorhizobium mediterraneum/temperatum group, wherea ...200919416352
robinia pseudoacacia in poland and japan is nodulated by mesorhizobium amorphae strains.robinia pseudoacacia microsymbionts from plants growing in poland and japan were evaluated for phylogeny and taxonomic position by genomic approach. based on the comparative analyses of atpd (368 bp) and dnak (573 bp) gene sequences as well as 16s rdna restriction analysis (rflp-16s rdna), r. pseudoacacia microsymbionts were identified as mesorhizobium strains. in dnak and atpd gene phylograms r. pseudoacacia nodulators formed robust, monophyletic clusters with mesorhizobium species with the nuc ...201020087765
draft genome sequence of plant growth-promoting rhizobium mesorhizobium amorphae, isolated from zinc-lead mine tailings.here, we describe the draft genome sequence of mesorhizobium amorphae strain ccnwgs0123, isolated from nodules of robinia pseudoacacia growing on zinc-lead mine tailings. a large number of metal(loid) resistance genes, as well as genes reported to promote plant growth, were identified, presenting a great future potential for aiding phytoremediation in metal(loid)-contaminated soil.201222247533
genomic analyses of metal resistance genes in three plant growth promoting bacteria of legume plants in northwest mine tailings, china.to better understand the diversity of metal resistance genetic determinant from microbes that survived at metal tailings in northwest of china, a highly elevated level of heavy metal containing region, genomic analyses was conducted using genome sequence of three native metal-resistant plant growth promoting bacteria (pgpb). it shows that: mesorhizobium amorphae ccnwgs0123 contains metal transporters from p-type atpase, cdf (cation diffusion facilitator), hupe/urej and chr (chromate ion transpor ...201525597676
characterization and genus identification of rhizobial symbionts from caragana arborescens in western canada.naturally occurring nitrogen-fixing symbionts from root nodules of caragana (caragana arborescens) growing in central saskatchewan were isolated following surface sterilization of caragana root nodules and squashing and spreading of the contents on yeast extract - mannitol medium. the symbiotic nature of the strains was confirmed following inoculation onto surface-sterilized c. arborescens seed in a gnotobiotic leonard jar system. the rhizobium isolates from c. arborescens root nodules were inte ...201323750954
rhizobium leguminosarum symbiovar trifolii, ensifer numidicus and mesorhizobium amorphae symbiovar ciceri (or mesorhizobium loti) are new endosymbiotic bacteria of lens culinaris medik.a total of 142 rhizobial bacteria were isolated from root nodules of lens culinaris medik endemic to tunisia and they belonged to the species rhizobium leguminosarum, and for the first time to ensifer and mesorhizobium, genera never previously described as microsymbionts of lentil. phenotypically, our results indicate that l. culinaris medik strains showed heterogenic responses to the different phenotypic features and they effectively nodulated their original host. based on the concatenation of ...201627267929
phylogenetic diversity of ammopiptanthus rhizobia and distribution of rhizobia associated with ammopiptanthus mongolicus in diverse regions of northwest china.aiming to investigate the diversity and distribution of rhizobia associated with ammopiptanthus, an endangered evergreen legume widely distributed in deserts, we characterized a total of 219 nodule isolates from nine sampling sites in northwest china with different soil characteristics based upon restriction fragment length polymorphism (rflp) analysis of 16s ribosomal rna (rrna) and symbiotic genes (nodc and nifh). ten isolates representing different 16s rrna-rflp types were selected for furthe ...201627079453
copper tolerance mechanisms of mesorhizobium amorphae and its role in aiding phytostabilization by robinia pseudoacacia in copper contaminated soil.the legume-rhizobium symbiosis has been proposed as an important system for phytoremediation of heavy metal contaminated soils due to its beneficial activity of symbiotic nitrogen fixation. however, little is known about metal resistant mechanism of rhizobia and the role of metal resistance determinants in phytoremediation. in this study, copper resistance mechanisms were investigated for a multiple metal resistant plant growth promoting rhizobium, mesorhizobium amorphae 186. three categories of ...201525594414
identification of rhizobial strains nodulating egyptian grain legumes.fifty four bacterial strains were isolated from root nodules of the grain legumes cicer arietinum, lens esculentus, phaseolus vulgaris, pisum sativum, and vicia faba grown in cultivated lands of beni-suef governorate (egypt). repetitive extragenic palindromic (rep)-polymerase chain reaction (pcr) clustered the strains into 15 rep-pcr groups. the nearly complete sequence of the 16s rrna gene from a representative strain of each rep-pcr pattern showed that the strains were closely related to membe ...201324568031
profiling of differentially expressed genes in roots of robinia pseudoacacia during nodule development using suppressive subtractive hybridization.legume-rhizobium symbiosis is a complex process that is regulated in the host plant cell through gene expression network. many nodulin genes that are upregulated during different stages of nodulation have been identified in leguminous herbs. however, no nodulin genes in woody legume trees, such as black locust (robinia pseudoacacia), have yet been reported.201323776436
mesorhizobium shonense sp. nov., mesorhizobium hawassense sp. nov. and mesorhizobium abyssinicae sp. nov., isolated from root nodules of different agroforestry legume trees.a total of 18 strains, representing members of the genus mesorhizobium, obtained from root nodules of woody legumes growing in ethiopia, have been previously shown, by multilocus sequence analysis (mlsa) of five housekeeping genes, to form three novel genospecies. in the present study, the phylogenetic relationship between representative strains of these three genospecies and the type strains of their closest phylogenetic neighbours mesorhizobium plurifarium, mesorhizobium amorphae, mesorhizobiu ...201322941297
genetic diversity, community structure and distribution of rhizobia in the root nodules of caragana spp. from arid and semi-arid alkaline deserts, in the north of china.the genetic diversity of 88 caragana nodule rhizobial isolates, collected from arid and semi-arid alkaline sandy soils in the north of china, was assessed by pcr-rflp of the 16s rrna gene and the 16s-23s igs, as well as the phylogenies of housekeeping genes (atpd, glnii and reca) and symbiotic genes (nodc and nifh). of the 88 strains, 69 were placed in the genus mesorhizobium, 16 in rhizobium and 3 in bradyrhizobium. mesorhizobium amorphae, mesorhizobium septentrionale, mesorhizobium temperatum ...201222463809
phylogeny of symbiotic genes and the symbiotic properties of rhizobia specific to astragalus glycyphyllos l.the phylogeny of symbiotic genes of astragalus glycyphyllos l. (liquorice milkvetch) nodule isolates was studied by comparative sequence analysis of noda, nodc, nodh and nifh loci. in all these genes phylograms, liquorice milkvetch rhizobia (closely related to bacteria of three species, i.e. mesorhizobium amorphae, mesorhizobium septentrionale and mesorhizobium ciceri) formed one clearly separate cluster suggesting the horizontal transfer of symbiotic genes from a single ancestor to the bacteria ...201526496493
comparative study of chromium biosorption by mesorhizobium amorphae strain ccnwgs0123 in single and binary mixtures.the appearance of chromium in the aqueous effluent is a major concern for the modern industry. in this work, mesorhizobium amorphae strain ccnwgs0123 was investigated as a biosorbent to remove chromium from aqueous solutions. the optimum ph for cr(iii) and cr(vi) biosorption were 4 and 2, respectively. this isolate showed an experimental maximum cr(iii) adsorption capacity of 53.52 mg l(-1), while the result was 47.67 mg l(-1) for cr(vi), with an initial 100 mg l(-1) cr ions and 1.0 g l(-1) biom ...201223269638
insight into the evolutionary history of symbiotic genes of robinia pseudoacacia rhizobia deriving from poland and japan.the phylogeny of symbiotic genes of robinia pseudoacacia (black locust) rhizobia derived from poland and japan was studied by comparative sequence analysis of noda, nodc, nodh, and nifh loci. in phylogenetic trees, black locust symbionts formed a branch of their own suggesting that the spread and maintenance of symbiotic genes within robinia pseudoacacia rhizobia occurred through vertical transmission. there was 99-100% sequence similarity for noda genes of robinia pseudoacacia nodulators, 97-98 ...201020306024
structure of the o-polysaccharides of the lipopolysaccharides of mesorhizobium loti hambi 1148 and mesorhizobium amorphae atcc 19655 containing two o-methylated monosaccharides.the o-polysaccharide of mesorhizobium loti hambi 1148 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses, smith degradation, and (1)h and (13)c nmr spectroscopies, including 2d (1)h/(1)h cosy, tocsy, roesy, and h-detected (1)h/(13)c hsqc experiments. the o-polysaccharide was found to have a branched hexasaccharide-repeating unit of the following structure: [formula: see text] where 2-acetamido-2-deoxy-4-o-methyl-d-glucose (d-glcnac4me) a ...200919850286
horizontal gene transfer and recombination shape mesorhizobial populations in the gene center of the host plants astragalus luteolus and astragalus ernestii in sichuan, china.thirty-three rhizobial strains isolated from the root nodules of astragalus luteolus and astragalus ernestii growing on the west plateau at two different altitudes in sichuan province, china, were characterized by amplified rdna restriction analysis (ardra), amplified fragment length polymorphism (aflp), and by sequencing of rrs, glna, glnii and nifh. the ardra analysis revealed considerable genomic diversity. in aflp analysis, 20 of 33 astragalus rhizobia formed three distinct clades, with othe ...200919796139
survey of chickpea rhizobia diversity in portugal reveals the predominance of species distinct from mesorhizobium ciceri and mesorhizobium mediterraneum.several mesorhizobium species are able to induce effective nodules in chickpea, one of the most important legumes worldwide. our aims were to examine the biogeography of chickpea rhizobia, to search for a predominant species, and to identify the most efficient microsymbiont, considering portugal as a case study. one hundred and ten isolates were obtained from continental portugal and madeira island. the 16s ribosomal rna gene phylogeny revealed that isolates are highly diverse, grouping with mos ...200919468700
characteristics of the rhizobia associated with dalea spp. in the ordway, kellogg-weaver dunes, and hayden prairies.habitat fragmentation affects the biodiversity and function of aboveground organisms in natural ecosystems but has not been studied for effects on belowground species. in this paper, we consider the diversity of the rhizobia associated with the indigenous legume dalea purpurea in 3 residual prairie areas in minnesota and iowa. using dalea purpurea as a trap host, 218 rhizobia were recovered from these soils then characterized using boxa1r pcr. three major and 13 minor groups were distinguished b ...200515782230
phylogenetic diversity of rhizobial strains nodulating robinia pseudoacacia l.lack of knowledge exists regarding the diversity of rhizobial strains nodulating black locust (robinia pseudoacacia l.), which is a neophytic tree species widely distributed in europe. seventeen rhizobial strains isolated from nodules of black locust at a german location were examined by phenotypic characterization and 16s rdna analysis. the isolates were classified in nine 16s rdna genotypes using a set of seven endonucleases. based on rflp analysis and sequencing, the strains were shown to bel ...200011065378
diversity of rhizobia associated with amorpha fruticosa isolated from chinese soils and description of mesorhizobium amorphae sp. nov.fifty-five chinese isolates from nodules of amorpha fruticosa were characterized and compared with the type strains of the species and genera of bacteria which form nitrogen-fixing symbioses with leguminous host plants. a polyphasic approach, which included rflp of pcr-amplified 16s rrna genes, multilocus enzyme electrophoresis (mlee), dna-dna hybridization, 16s rrna gene sequencing, electrophoretic plasmid profiles, cross-nodulation and a phenotypic study, was used in the comparative analysis. ...199910028247
biosorption of copper (ii) from aqueous solution using non-living mesorhizobium amorphae strain ccnwgs0123.the mining industry generates huge amounts of wastewater, containing toxic heavy metals. treatment to remove heavy metals is necessary and recent work has been focused on finding more environmentally friendly materials for removing heavy metals from wastewater. biosorption can be an effective process for heavy metal removal from aqueous solutions. our objectives were to investigate the removal of copper (ii) from aqueous solutions using dead cells of mesorhizobium amorphae ccnwgs0123 under diffe ...201222353770
mesorhizobium waimense sp. nov. isolated from sophora longicarinata root nodules and mesorhizobium cantuariense sp. nov. isolated from sophora microphylla root nodules.in total 14 strains of gram-stain-negative, rod-shaped bacteria were isolated from sophora longicarinata and sophora microphylla root nodules and authenticated as rhizobia on these hosts. based on the 16s rrna gene phylogeny, they were shown to belong to the genus mesorhizobium, and the strains from s. longicarinata were most closely related to mesorhizobium amorphae accc 19665t (99.8–99.9 %), mesorhizobium huakuii iam 14158t (99.8–99.9 %), mesorhizobium loti usda 3471t (99.5–99.9 %) and mesorhi ...201526296780
multilocus sequence analysis supports the taxonomic position of astragalus glycyphyllos symbionts based on dna-dna hybridization.in this study, the phylogenetic relationship and taxonomic status of six strains, representing different phenons and genomic groups of astragalus glycyphyllos symbionts, originating from poland, were established by comparative analysis of five concatenated housekeeping gene sequences (atpd, dnak, glna, reca and rpob), dna-dna hybridization and total dna g+c content. maximum-likelihood phylogenetic analysis of combined atpd, dnak, glna, reca and rpob sequence data placed the studied bacteria into ...201626704062
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