| purification and partial characterization of the plasma clotting protein from the pink shrimp farfantepenaeus paulensis. | a clotting protein (cp) was purified from the plasma of the pink shrimp farfantepenaeus paulensis by sequential anion-exchange chromatography. the shrimp cp was able to form stable clots in vitro in the presence of hemocyte lysate and ca2+, suggesting that the clotting reaction is catalyzed by a ca2+-dependent transglutaminase present in shrimp hemocytes. dansylcadaverine was incorporated into the shrimp cp in the presence of endogenous transglutaminase (hemocyte lysate), confirming that the shr ... | 2005 | 16153866 |
| expression and characterisation of tiger shrimp penaeus monodon penaeidin (mo-penaeidin) in various tissues, during early embryonic development and moulting stages. | a penaeidin family, mo-penaeidin was cloned from the haemocytes of tiger shrimp penaeus monodon using genomic polymerase chain reaction (pcr) by gene specific primers. analysis of nucleotide sequence revealed that this mo-penaeidin consists of 1348 bp containing one intron (680 bp) and two exons (210 and 458 bp). it has an open reading frame (orf) of 222 p, which encodes a protein of 74 amino acids including a signal peptide of 19 amino acids. the calculated molecular mass of the mature protein ... | 2007 | 16820207 |
| Alpha2-macroglobulin from an Atlantic shrimp: biochemical characterization, sub-cellular localization and gene expression upon fungal challenge. | In this study, we report on the isolation and characterization of an alpha2-macroglobulin (a2M) from the plasma of the pink shrimp Farfantepenaeus paulensis, its sub-cellular localization and transcriptional changes after infection by fungi. The molecular mass of the a2M was estimated at 389 kDa by gel filtration and 197 kDa by SDS-PAGE, under reducing conditions, suggesting that a2M from F. paulensis consists of two identical sub-units, covalently linked by disulphide bonds. The N-terminal amin ... | 2011 | 21888978 |
| molecular cloning of crustins from the hemocytes of brazilian penaeid shrimps. | crustins are antimicrobial peptides initially identified in the hemocytes of the crab carcinus maenas (11.5-kda peptide or carcinin) and recently also recognized in penaeid shrimps and other crustacean species. the aim of this study was to identify sequences encoding for crustins from the hemocytes of four brazilian penaeid species: farfantepenaeus paulensis, farfantepenaeus subtilis, farfantepenaeus brasiliensis and litopenaeus schmitti. using primers based on consensus nucleotide alignment of ... | 2007 | 17697082 |
| sperm ultrastructure of shrimp from family penaeidae (crustacea: dendrobranchiata) in a phylogenetic context. | we describe the sperm ultrastructure of six penaeid species, including at least one member of each tribe (penaeini, parapenaeini and trachypenaeini). fragments of the vas deferens of the penaeidae farfantepenaeus brasiliensis, farfantepenaeus paulensis, litopenaeus schmitti, parapenaeus americanus, rimapenaeus constrictus and xiphopenaeus kroyeri were fixed and processed according to the routine for transmission electron microscopy. the morphological results were contextualized in an evolutionar ... | 2017 | 28163192 |
| the use of oxygen consumption and ammonium excretion to evaluate the toxicity of cadmium on farfantepenaeus paulensis with respect to salinity. | the main purpose of the present study was to detect the acute toxicity of cadmium (cd) in f. paulensis and to investigate its effect on oxygen consumption and ammonium excretion different salinities. first, we examined the acute toxicity of cd in f. paulensis at 24, 48, 72, and 96-h lethal concentration (lc50). cd was significantly more toxic at 5 salinity than at 20 and 36. the oxygen consumption and ammonium excretion were estimated through experiments performed on each of the twelve possible ... | 2011 | 21477843 |
| effects of zinc and cadmium on oxygen consumption and ammonium excretion in pink shrimp (farfantepenaeus paulensis, pérez-farfante, 1967, crustacea). | in brazil, pink shrimp (farfantepenaeus paulensis) is an important commercially exploited species and is an ideal animal for studying the impairment caused by the effects of heavy metals that are often detected in coastal areas. the main purpose of the present study was to detect the acute toxicity of cadmium and zinc to f. paulensis and investigate their effects on oxygen consumption and ammonium excretion, investigations that have not been carried out in this species before. first, the acute t ... | 2009 | 19031115 |
| stock assessment and fishery management of the pink shrimp farfantepenaeus brasiliensis latreille, 1970 and f. paulensis pérez-farfante, 1967 in southeastern brazil (23 degrees to 28 degrees s). | quantitative analyses of the pink shrimp (farfantepenaeus brasiliensis and farfantepenaeus paulensis) fisheries were carried out using data collected from july 1999 to july 2001 from the trawling operations of the fishing fleet based in santos/guarujá, sp. according to classical models, the fishery is at its maximum sustainable yield. therefore, reduction of the fishing effort and adequate season and area closures seem to be the best management actions for the pink shrimp fishery. | 2006 | 16710519 |
| simultaneous measurements of oxygen consumption and ammonia-n excretion in embryos and larvae of marine invertebrates. | the quantification of oxygen consumption and ammonia-n excretion rates is essential in determining energy requirements for development of larval invertebrates. in larval energetics, there is a need for accurate and uncomplicated techniques to quantify metabolic rates. a method for simultaneous measurements of oxygen and ammonia-n concentrations is presented. it employs sealed respirometric chambers (ca. 30 ml) in which embryos and larvae are incubated. analysis is carried out in end-point sample ... | 2003 | 14511751 |
| [characterization of the lipid portion of pink shrimp commercial samples]. | the objective of this study was to describe the fresh cooled pink-shrimp (penaeus brasiliensis and penaeus paulensis) lipid. the total lipid content was 1.13 +/- 0.09 g/100 g while fatty acid profile showed 32.9% saturated, 20.4% monounsaturated and 40.5% polyunsaturated. eighteen fatty acids were detected, seven saturated (14:0, 15:0, 16:0, 17:0, 18:0, 20:0, 22:0), four monounsaturated (16:1w7, 17:1w9, 18:1w9, 20:1w9) and seven polyunsaturated (18:2w6, 18:3w3, 20:2w6, 20:4w6, 20:5w3, 22:5w3, 22 ... | 2002 | 12184157 |
| effects of copper and zinc on growth, feeding and oxygen consumption of farfantepenaeus paulensis postlarvae (decapoda: penaeidae). | the effect of chronic exposure (35 days) to sub-lethal concentrations of copper (17-212 ppb) and zinc (41-525 ppb) on growth of farfantepenaeus paulensis postlarvae 17 days old (pl(17)) was analysed. the effects of acute exposure of pl(17) to the same metal on food ingestion and oxygen consumption were also evaluated. studies were performed using copper and zinc singly, and in a mixture of equipotent concentrations (1:2.5). chronic exposure to copper (85 and 212 ppb) and zinc (106, 212 and 525 p ... | 2000 | 10742507 |
| first report of white spot syndrome virus in farmed and wild penaeid shrimp from lagoa dos patos estuary, southern brazil. | in this study, we detected white spot syndrome virus (wssv) in wild farfantepenaeus paulensis collected in the lagoa dos patos estuary and cultivated litopenaeus vannamei. this is the first report of wssv in f. paulensis from lagoa dos patos and farmed l. vannamei shrimps in rio grande do sul. | 2011 | 24031739 |
| hemocytes of the palaemonids macrobrachium rosenbergii and m. acanthurus, and of the penaeid penaeus paulensis. | the hemocytes of two palaemonids and one penaeid were characterized using light and transmission electron microscopy (tem). the blood cells in all three species were classified as hyaline hemocytes (hh), small granule hemocytes (sgh), and large granule hemocytes (lgh). the hh are unstable hemocytes with a characteristic high nucleo-cytoplasmic ratio. their cytoplasm appears particularly dense and has from few to numerous granules that often exhibit a typical striated substructure. in both palaem ... | 1998 | 9606943 |
| a resource management scenario for traditional and scientific management of pink shrimp (farfantepenaeus paulensis) in the patos lagoon estuary (rs), brazil. | this article aims to discuss the incorporation of traditional time in the construction of a management scenario for pink shrimp in the patos lagoon estuary (rs), brazil. to meet this objective, two procedures have been adopted; one at a conceptual level and another at a methodological level. at the conceptual level, the concept of traditional time as a form of traditional ecological knowledge (tek) was adopted. | 2013 | 23311826 |
| evidence for a novel biological role for the multifunctional β-1,3-glucan binding protein in shrimp. | β-1,3-glucan binding proteins (βgbps) are soluble pattern recognition proteins/receptors that bind to β-1,3-glucans from fungi cell walls. in crustaceans, βgbps are abundant plasmatic proteins produced by the hepatopancreas, and have been proved to play multiple biological functions. here, we purified and characterized novel members of the βgbp family from the hemolymph of two brazilian shrimps, farfantepenaeus paulensis (fpβgbp) and litopenaeus schmitti (lsβgbp). as observed for other crustacea ... | 2012 | 22525007 |
| a novel monoclonal antibody that binds to hemocytes from shrimps and oysters. | the monoclonal antibody (mab) lito-1 was produced from a stable hybridoma cell line generated by the fusion of ns1 myeloma cells with spleen cells isolated from balb/c mice immunized with a paraformaldehyde-fixed hemocyte suspension of litopenaeus vannamei. this mab reacted with all three hemocyte subtypes, but no reaction was observed with components of plasma. immunohistochemistry assays demonstrated that lito-1 was very effective in specifically distinguishing hemocytes infiltrated in several ... | 2010 | 20443709 |