| vibrio halioticoli sp. nov., a non-motile alginolytic marine bacterium isolated from the gut of the abalone haliotis discus hannai. | six alginolytic, facultatively anaerobic, non-motile marine bacteria were isolated from the gut of abalone haliotis discus hannai. dna-dna hybridization data showed that the six strains constituted a single genospecies. phylogenetic analyses of 16s rdna sequences indicated that the isolates should be assigned to the genus vibrio. the phenotypic features of the isolates were closely related to vibrio fischeri and vibrio pelagius biovar i, but 13 traits (motility, luminescence, alginase production ... | 1998 | 9731299 |
| cloning and sequence analysis of vibrio halioticoli genes encoding three types of polyguluronate lyase. | the alginate lyase-coding genes of vibrio halioticoli iam 14596(t), which was isolated from the gut of the abalone haliotis discus hannai, were cloned using plasmid vector puc 18, and expressed in escherichia coli. three alginate lyase-positive clones, pvhb, pvhc, and pvhe, were obtained, and all clones expressed the enzyme activity specific for polyguluronate. three genes, alyvg1, alyvg2, and alyvg3, encoding polyguluronate lyase were sequenced: alyvg1 from pvhb was composed of a 1056-bp open r ... | 2000 | 10804241 |
| in situ polymerase chain reaction visualization of vibrio halioticoli using alginate lyase gene alyvg2. | a prokaryotic in situ polymerase chain reaction (pi-pcr) technique was applied to visualize vibrio halioticoli cells using alginate lyase gene alyvg2 as a target gene. prior to pi-pcr, a primer set, vg2-os3, for specific amplification of an approximately 1.0-kb fragment from v. halioticoli genomic dna was developed with amplified fragments from v. pelagius and v. fischeri dnas as reference strains. one-stage pi-pcr using the primer set, digoxigenin-labeled dutp, and indirect alkaline-phosphatase ... | 2000 | 10804242 |
| fluorescent amplified fragment length polymorphism and repetitive extragenic palindrome-pcr fingerprinting reveal host-specific genetic diversity of vibrio halioticoli-like strains isolated from the gut of japanese abalone. | when analyzed by fluorescent amplified fragment length polymorphism and repetitive extragenic palindrome-pcr fingerprinting, a total of 47 vibrio halioticoli strains isolated from four japanese abalone species and one turban shell species formed three clusters that roughly reflect the different species of host abalone from which they were isolated. the v. halioticoli isolates from turban shells were distributed evenly among the clusters. representative isolates from two clusters were deemed sepa ... | 2002 | 12147522 |
| vibrio superstes sp. nov., isolated from the gut of australian abalones haliotis laevigata and haliotis rubra. | five alginolytic, facultatively anaerobic, non-motile bacteria were isolated from the gut of abalones haliotis laevigata and haliotis rubra. phylogenetic analyses based on 16s rdna data indicated that these strains are related closely to vibrio halioticoli (98 % 16s rdna sequence similarity). dna-dna hybridization and fluorescent amplified fragment length polymorphism fingerprinting demonstrated that the five strains constituted a single species that was different from all currently known vibrio ... | 2003 | 14657109 |
| vibrio gallicus sp. nov., isolated from the gut of the french abalone haliotis tuberculata. | five alginolytic, facultatively anaerobic, non-motile bacteria were isolated from the gut of the abalone haliotis tuberculata. phylogenetic analyses based on 16s rdna data indicated that these strains are related to vibrio wodanis, vibrio salmonicida, vibrio logei and vibrio fischeri (but with <97 % 16s rrna gene sequence similarity). dna-dna hybridization and fluorescence amplified fragment length polymorphism fingerprinting demonstrated that the five strains constituted a single species that w ... | 2004 | 15143033 |
| phylogenetic analysis of vibrios and related species by means of atpa gene sequences. | we investigated the use of atpa gene sequences as alternative phylogenetic and identification markers for vibrios. a fragment of 1322 bp (corresponding to approximately 88% of the coding region) was analysed in 151 strains of vibrios. the relationships observed were in agreement with the phylogeny inferred from 16s rrna gene sequence analysis. for instance, the vibrio cholerae, vibrio halioticoli, vibrio harveyi and vibrio splendidus species groups appeared in the atpa gene phylogenetic analyses ... | 2007 | 17978204 |
| antibacterial and antilarval-settlement potential and metabolite profiles of novel sponge-associated marine bacteria. | in this study, we screened seven novel sponge-associated marine bacteria for their antibacterial and antilarval-settlement activity in order to find possible new sources of non-toxic or less toxic bioactive antifoulants. the anti-bacterial-growth activity of crude extracts of each bacterium was evaluated by the disk-diffusion assay. extracts of four potent bacteria with high and broad spectra of antibacterial activity were further separated with solvents of different polarities (hexane and ethyl ... | 2009 | 19471982 |
| vibrio breoganii sp. nov., a non-motile, alginolytic, marine bacterium within the vibrio halioticoli clade. | seven non-motile, facultatively anaerobic, alginolytic marine bacteria were isolated from the cultured clams ruditapes philippinarum and ruditapes decussatus. phylogenetic analysis based on 16s rrna gene sequences showed that these marine bacteria were closely related to the recently described species vibrio comitans, vibrio rarus and vibrio inusitatus (>or=99.0 % sequence similarity). phylogenetic analysis based on the housekeeping genes rpoa, reca and atpa grouped the isolates together and all ... | 2009 | 19542146 |
| vibrio ishigakensis sp. nov., in halioticoli clade isolated from seawater in okinawa coral reef area, japan. | five novel strains showing non-motile, alginolytic, halophilic and fermentative features were isolated from seawater samples off okinawa in coral reef areas. these strains were characterized by an advanced polyphasic taxonomy including genome based taxonomy using multilocus sequence analysis (mlsa) and in silico dna-dna similarity (in silico ddh). phylogenetic analyses on the basis of 16s rrna gene sequences revealed that the isolates could be assigned to the genus vibrio, however they were not ... | 2016 | 27262360 |