| first description of grapevine leafroll-associated virus 5 in argentina and partial genome sequence. | an accession of vitis vinifera cv. red globe from argentina, was found to be infected with grapevine leafroll-associated virus-5 by elisa. it was partially sequenced, and three orfs, corresponding to hsp70h, hsp90h, and cp, were found. this isolate shares a high aminoacid identity with the previously reported sequence of the virus, and identities between 80% and 90% with previously reported glrav-9 and glrav-4 isolates. the analysis of the sequence supports the clustering together with glrav-4 a ... | 2009 | 18953643 |
| occurrence of grapevine leafroll-associated virus 5 in portugal: genetic variability and population structure in field-grown grapevines. | a comparison of 15 field isolates of grapevine leafroll-associated virus 5 (glrav-5) was conducted, based on the analysis of nucleotide sequences of two viral orfs: the coat protein (cp) and the heat shock protein 90 homolog (hsp90h). after amplification and cloning, the population of viral sequences was analyzed for each isolate, revealing the within-isolate presence of sequence variants for both genes, with one or more major cp variants. phylogenetic analysis showed the gene sequence variants ... | 2012 | 22692680 |
| genomic analysis of grapevine leafroll associated virus-5 and related viruses. | the grapevine leafroll-associated viruses (glravs) (closteroviridae) represent an emerging threat to world grape production. one group of glravs within the genus ampelovirus, the glrav-4-like viruses (glrav-4lvs), contains a fragmented collection of seven viruses only two of which (glrav-pr and glracv) are fully sequenced. here in reporting the sequence of glrav-5, a member of glrav-4lvs, we identify genomic elements common to the glrav-4lv group. exclusive properties include a highly conserved ... | 2011 | 21893115 |
| a strategy for rapid cdna cloning from double-stranded rna templates isolated from plants infected with rna viruses by using taq dna polymerase. | a fast and efficient cdna cloning procedure for plant rna viruses was developed. in this procedure, double-stranded rna (dsrna) was used as a template source. standard cdna synthesis reagents and random hexamers were then used for making cdnas. taq dna polymerase was used to add additional (a) at the ends of cdnas, a ta cloning kit to ligate the cdnas to vectors, and an electroporator to transform the dnas to e. coli cells. dsrnas were extracted from grapevine tissues infected with four differen ... | 2000 | 10644087 |
| partial genome organization, identification of the coat protein gene, and detection of grapevine leafroll-associated virus-5. | abstract the genome of grapevine leafroll-associated virus-5 (glrav-5) was cloned, and the sequence of 4766 nt was determined. degenerate oligonucleotide primers designed from the conserved closterovirus heat shock 70 protein (hsp 70) homologue were used to obtain viral-specific sequences to anchor the cloning of the viral rna with a genomic walking approach. the partial nucleotide (nt) sequence of glrav-5 showed the presence of four open reading frames (orf a through d), potentially coding for ... | 2001 | 18943347 |