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aeromonas popoffii sp. nov., a mesophilic bacterium isolated from drinking water production plants and reservoirs.we examined the taxonomic position of seven aeromonas isolates, recovered from flemish and scottish drinking water production plants and reservoirs, which were previously recognized by numerical analysis of genomic aflp fingerprints as members of an unknown aeromonas taxon that most closely resembled the species aeromonas bestiarum (dna hybridization group [hg] 2). the new phenotypic and dna-dna hybridization data obtained in this study show that the a. bestiarum-like strains constitute a separa ...19979336924
signature region within the 16s rdna sequences of aeromonas popoffii.to identify a group of eight aeromonas strains of our collection showing ribotyping patterns similar to those described for the species aeromonas popoffii, 16s rrna gene sequence analysis was performed. results were in agreement with the dna binding values, and allowed the identification of a 'signature region' differentiating the a. popoffii strains from all other members of the genus aeromonas.199910188252
phylogenetic positions of aeromonas encheleia, aeromonas popoffii, aeromonas dna hybridization group 11 and aeromonas group 501.the 16s rdna sequences of the recently described aeromonas encheleia and aeromonas popoffii, were determined and compared with data from all known aeromonas sp. diagnostic 16s rdna regions were also sequenced for some strains previously considered as an extension of a. encheleia and a strain of aeromonas group 501 (formerly enteric group 501). results indicated that a. encheleia and a. popoffii are phylogenetically separated species as originally described. a conclusion about hg11 taxonomic stat ...199910555319
epidemiological relationships between aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping.ribotyping was used to study the epidemiology of aeromonas associated gastro-enteritis in young children. ribotyping patterns of 29 aeromonas strains (16 aeromonas caviae, 8 aeromonas hydrophila, 3 aeromonas eucrenophila, 1 aeromonas veronii, and 1 aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the gelcompare software with patterns of 104 strains (39 aeromonas eucrenophila, 29 aeromonas caviae, 11 aeromonas encheleia, 10 aeromonas hydrophila, 6 aer ...200010997832
extended method for discrimination of aeromonas spp. by 16s rdna rflp analysis.a previously described molecular method, based on 16s rdna rflp analysis, for the identification of aeromonas spp. was unable to separate the species aeromonas salmonicida, aeromonas bestiarum and the recently described aeromonas popoffii. in this study, the method has been extended with endonucleases aiwni and psti for the identification of these species. a molecular frame for the identification of all known aeromonas spp. is presented.200011155981
potential virulence and antimicrobial susceptibility of aeromonas popoffii recovered from freshwater and seawater.aeromonas popoffii is the most recent species within the genus aeromonas described from freshwater. in our study this species was also recovered from this habitat and for the first time from seawater. most of the virulence factors known in aeromonas spp. (aerolysin/hemolysin, serine protease, lipases and dnases) were highly prevalent in this species. third-generation cephalosporins and quinolones were the most active antimicrobial agents against a. popoffii.200211934570
comparison of three molecular methods for typing aeromonas popoffii isolates.three typing methods, restriction fragment length polymorphism (rflp) of the 16s-23s intergenic spacer region (isr), pcr amplification of the enterobacterial repetitive intergenic consensus (eric) and of the repetitive extragenic palindromic units (rep), were evaluated for typing 26 isolates of aeromonas popoffii from different geographical origins. when the methods were independently studied, eric showed the highest discriminatory power. when the methods were combined, the best combination of t ...200312777070
aeromonas popoffii urinary tract infection.aeromonas popoffii is a recently described species isolated mainly from freshwater. an isolate of aeromonas popoffii was found to be responsible for a urinary tract infection in a 13-year-old boy suffering from spina bifida with enterocystoplasty. this is the first reported case of human infection attributed to this species.200415528763
rabbit meat as a source of bacterial foodborne pathogens.even though worldwide production of rabbit meat is >1,000,000 tons, little information is available for rabbit meat microbiology. this study provides data on the prevalence of salmonella, escherichia coli o157:h7, yersinia enterocolitica, listeria spp., motile aeromonas spp., and staphylococcus aureus on rabbit meat. a total of 24 rabbit carcasses from two abattoirs and 27 rabbit meat packages from supermarket displays were examined. in addition to culturing methods, associated virulence genes w ...200616715811
aeromonas sharmana sp. nov., isolated from a warm spring.a gram-negative, facultatively anaerobic bacterial strain designated gptsa-6(t) was isolated from a water sample collected from a warm spring in assam, india. preliminary analysis of the 16s rrna gene sequence of this isolate revealed its affiliation to the family aeromonadaceae. detailed characterization using a polyphasic approach indicated that strain gptsa-6(t) is most closely related to aeromonas sobria but differs significantly from existing members of the genus aeromonas. analysis of the ...200616902029
aeromonas piscicola sp. nov., isolated from diseased fish.four aeromonas strains (s1.2(t), eo-0505, tc1 and ti 1.1) isolated from moribund fish in spain showed a restriction fragment length polymorphism (rflp) pattern related to strains of aeromonas salmonicida and aeromonas bestiarum but their specific taxonomic position was unclear. multilocus sequence analysis (mlsa) of housekeeping genes rpod, gyrb, reca and dnaj confirmed the allocation of these isolates to an unknown genetic lineage within the genus aeromonas with a. salmonicida, a. bestiarum and ...200919570633
mechanisms of quinolone resistance in aeromonas species isolated from humans, water and eels.mechanisms of resistance were determined in 33 quinolone-resistant isolates of the species aeromonas hydrophila, aeromonas caviae, aeromonas media, aeromonas salmonicida, aeromonas popoffii and aeromonas veronii, recovered from humans, freshwater and eels. the quinolone resistance-determining regions (qrdrs) of gyra and parc genes were sequenced in these resistant strains, as well as in 8 quinolone-sensitive aeromonas used as controls. all quinolone-resistant aeromonas carried point mutations in ...201019900538
polymerase chain reaction-denaturing gradient gel electrophoresis analysis of bacterial community structure in the food, intestines, and feces of earthworms.the bacterial communities in the food, intestines, and feces of earthworms were investigated by pcr-denaturing gradient gel electrophoresis (dgge). in this study, pcr-dgge was optimized by testing 6 universal primer sets for microbial 16s rrna in 6 pure culture strains of intestinal microbes in earthworms. one primer set effectively amplified 16s rrna from bacterial populations that were found in the food, intestines, and feces of earthworms. compared with the reference markers from the pure cul ...201121887635
antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia).antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ...201525803299
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