| purification and characterization of ovine astrovirus. | astrovirus, purified in aggregated form from epithelial cells of the small intestinal villi of infected gnotobiotic lambs, was shown to have a single-stranded rna genome with an s value of 34 and poly(a) tract. only two major capsid polypeptides were detected with similar mol. wt. of approx. 3.3 x 10(4). | 1981 | 6168736 |
| evolutionary relationships among astroviridae. | to study the evolutionary relationships among astroviruses, all available sequences for members of the family astroviridae were collected. phylogenetic analysis distinguished two deep-rooted groups: one comprising mammalian astroviruses, with ovine astrovirus being an outlier, and the other comprising avian astroviruses. all virus species as well as serotypes of human astroviruses represented individual lineages within the tree. all human viruses clustered together and separately from non-human ... | 2002 | 12029155 |
| molecular characterization of a novel astrovirus associated with disease in mink. | pre-weaning diarrhoea is a well-known problem in mink farming in europe, causing morbidity that varies between farms, regions and season. different causalities for the disease have been proposed, but only most recently has a novel astrovirus been identified as an important risk factor. in this report, the molecular characterization, origin and evolution of this novel astrovirus of mink are discussed. the polyadenylated, positive-stranded rna genome was sequenced and found to contain 6610 nt, org ... | 2003 | 14573813 |
| isolation and phylogenetic characterization of bat astroviruses in southern china. | astroviruses are associated with acute gastroenteritis of human and many animal species. recently, two studies have reported that novel astroviruses were found in bats. in order to extensively understand the genetic and phylogenetic characterization of bat astroviruses, we tested fecal samples of nine bat species that were collected at four natural habitats in three areas of southern china. the geographic distributions of the bats involved differed from previous reports. three out of nine specie ... | 2011 | 21573690 |
| identification of a novel astrovirus in domestic sheep in hungary. | the family astroviridae consists of two genera, avastrovirus and mamastrovirus, whose members are associated with gastroenteritis in avian and mammalian hosts, respectively. we serendipitously identified a novel ovine astrovirus in a fecal specimen from a domestic sheep (ovis aries) in hungary by viral metagenomic analysis. sequencing of the fragment indicated that it was an orf1b/orf2/3'utr sequence, and it has been submitted to the genbank database as ovine astrovirus type 2 (oastv-2/hungary/2 ... | 2011 | 22033597 |
| divergent astrovirus associated with neurologic disease in cattle. | using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (boastv-neuros1) that was phylogenetically related to an ovine astrovirus. in a retrospective analysis of 32 cases of bovine encephalitides of unknown etiology, 3 other infected animals were detected by using pcr and in situ hybridization for viral rna. viral rna was restricted to the nervous system and detected in the cytopla ... | 0 | 23965613 |
| a novel astrovirus associated with encephalitis and ganglionitis in domestic sheep. | in june 2013, a 4-year-old welsh mountain ewe and in march 2014 a 10-day-old lamb of the same breed and the same flock presented progressive neurological signs including depressed sensorium, tremor, and unusual behaviour. neuropathological examination of the brain and spinal cord detected non-suppurative polioencephalomyelitis and dorsal root ganglionitis, characteristic of a neurotropic viral agent in both sheep. metagenomic analysis of different tissue samples from both animals identified a no ... | 2017 | 28224712 |
| full genome analysis of bovine astrovirus from fecal samples of cattle in japan: identification of possible interspecies transmission of bovine astrovirus. | a viral metagenomics approach was used to investigate fecal samples of japanese calves with and without diarrhea. of the different viral pathogens detected, read counts gave nearly complete astrovirus-related rna sequences in 15 of the 146 fecal samples collected in three distinct areas (hokkaido, ishikawa, and kagoshima prefectures) between 2009 and 2015. due to the lack of genetic information about bovine astroviruses (boastvs) in japan, these sequences were analyzed in this study. nine of the ... | 2015 | 26212364 |
| phylogenetic analysis of bovine astrovirus in korean cattle. | bovine astrovirus (bastv) belongs to a genetically divergent lineage within the genus mamastrovirus. the present study showed that bastv was associated with the gastroenteric tracts of cattle in nine positive fecal samples from 115 cattle, whereas no positive samples were found in the brain tissues of 14 downer cattle. interestingly, the positive diarrheal samples were obtained mainly from calves aged 14 days-3 months. bayesian inference tree analysis of the partial orf1ab and capsid (orf2) gene ... | 2014 | 24272699 |
| novel encephalomyelitis-associated astrovirus in a muskox (ovibos moschatus): a surprise from the archives. | the small, single-stranded positive-sense rna astroviruses are mostly known to be enteric viruses. in recent years, though, different astroviruses were reported in association with neurological disease in various species. in cattle, two distinct neurotropic astrovirus genotype species were described in numerous cases of nonsuppurative encephalomyelitis, with one of these viruses also reported in similar circumstances in several sheep. here, we retrieved archived formalin-fixed, paraffin-embedded ... | 2019 | 31234899 |