selective removal of nitrogen from quinoline and petroleum by pseudomonas ayucida igtn9m.enrichment culture experiments employing soil and water samples obtained from petroleum-contaminated environments succeeded in the isolation of a pure culture possessing the ability to utilize quinoline as a sole nitrogen source but did not utilize quinoline as a carbon source. this culture was identified as pseudomonas ayucida based on a partial 16s rrna gene sequence, and the strain was given the designation igtn9m. examination of metabolites using thin-layer chromatography and gas chromatogra ...200010653737
isolation of bacteriophages specific to a fish pathogen, pseudomonas plecoglossicida, as a candidate for disease control.two types of bacteriophage specific to pseudomonas plecoglossicida, the causative agent of bacterial hemorrhagic ascites disease in cultured ayu fish (plecoglossus altivelis), were isolated from diseased ayu and the rearing pond water. one type of phage, which formed small plaques, was tentatively classified as a member of the family myoviridae, and the other type, which formed large plaques, was classified as a member of the family podoviridae. all 27 strains of p. plecoglossicida examined, whi ...200010742221
pseudomonas plecoglossicida sp. nov., the causative agent of bacterial haemorrhagic ascites of ayu, plecoglossus altivelis.a new pseudomonas species, for which the name pseudomonas plecoglossicida is proposed, was isolated from cultured ayu (plecoglossus altivelis) with bacterial haemorrhagic ascites. the causative agent was similar to pseudomonas putida biovar a in its phenotypic characteristics and on the basis of 16s rrna gene sequence analysis, but it reduced nitrate to nitrite. furthermore, it was distinguished phenotypically from pseudomonas putida biovar a by utilization of d-malate, l-(+)-tartrate, m-tartrat ...200010826790
siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads.a total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. as a general rule, strains within a well-circumscribed taxonomic group, namely the species pseudomonas brassicacearum, pseudomonas fuscovaginae, pseudo ...200212039729
bacteriophage control of pseudomonas plecoglossicida infection in ayu plecoglossus altivelis.two previously isolated phages were used to examine the therapeutic effects against pseudomonas plecoglossicida infection in ayu plecoglossus altivelis. phage ppp-w4 (podoviridae) inhibited the in vitro growth of p. plecoglossicida more effectively than phage pppw-3 (myoviridae), and a mixture (pppw-3/w-4) of the 2 phages exhibited the highest inhibitory activity. in phage therapy experiments, ayu were fed p. plecoglossicida-impregnated feed (10(7) cfu fish(-1)) and then fed phage-impregnated fe ...200312608566
histopathological studies of bacterial haemorrhagic ascites of ayu, plecoglossus altivelis (temminck & schlegel).a histopathological study was carried out on ayu, plecoglossus altivelis, with bacterial haemorrhagic ascites. the fish were obtained from culture ponds in wakayama prefecture in 2003. the causative agent was identified as pseudomonas plecoglossicida by a slide agglutination test using anti-p. plecoglossicida fpc941 serum. histopathological studies revealed lesions in spleen, kidney, liver, intestine, heart and gills. lesions in the spleen and haematopoietic tissue were prominent and invaded by ...200415291787
formation of water-soluble metal cyanide complexes from solid minerals by pseudomonas plecoglossicida.a few pseudomonas species are able to form hydrocyanic acid (hcn), particularly when grown under glycine-rich conditions. in the presence of metals, cyanide can form water-soluble metal complexes of high chemical stability. we studied the possibility to mobilize metals as cyanide complexes from solid minerals using hcn-forming microorganisms. pseudomonas plecoglossicida was cultivated in the presence of copper- and nickel-containing solid minerals. on powdered elemental nickel, fast hcn generati ...200616684101
identification and detection of pseudomonas plecoglossicida isolates with pcr primers targeting the gyrb region.pseudomonas plecoglossicida is the agent of bacterial haemorrhagic ascites (bha) in freshwater fish farming in japan. to develop a rapid identification and detection method for p. plecoglossicida, a pcr amplification technique targeting the chromosomal dna region coding the b subunit of the dna gyrase (gyrb) was used. the nucleotide sequences of gyrb were determined in nine isolates of p. plecoglossicida and two other pseudomonas species. on the basis of these determined sequences and the gyrb s ...200717584436
characterization of bacillus and pseudomonas strains with suppressive traits isolated from tomato hydroponic-slow filtration unit.bacillus and pseudomonas spp. are known to be involved in plant pathogenic fungi elimination during the slow filtration process used in tomato soilless cultures. we isolated 6-8 strains of both bacillus and pseudomonas from the top, middle, and bottom sections of filters and identified them after 16s rdna sequencing. four pseudomonas strains were identified as pseudomonas fulva, 5 as pseudomonas plecoglossicida, and 12 as pseudomonas putida. the use of specific oligonucleotide polymerase chain r ...200717668039
establishment and characterization of dual-species biofilms formed from a 3,5-dinitrobenzoic-degrading strain and bacteria with high biofilm-forming this study, the possibility of establishing a dual-species biofilm from a bacterium with a high biofilm-forming capability and a 3,5-dinitrobenzoic acid (3,5-dnba)-degrading bacterium, comamonas testosteroni a3, was investigated. our results showed that the combinations of strain a3 with each of five strains with a high biofilm-forming capability (pseudomonas sp. m8, pseudomonas putida m9, bacillus cereus m19, pseudomonas plecoglossicida m21 and aeromonas hydrophila m22) presented different l ...200818034834
expression of arginine deiminase from pseudomonas plecoglossicida cgmcc2039 in escherichia coli and its anti-tumor activity.arginine deiminase (adi), an arginine-degrading enzyme, has been studied as a potential anti-cancer agent in clinical trials for the treatment of arginine-auxotrophic tumors, such as hepatocellular carcinomas (hccs) and melanomas. the arca gene encoding adi was cloned from a recently isolated strain pseudomonas plecoglossicida cgmcc2039. the nucleotide sequence of adi comprises an orf of 1,254 bp encoding 417 amino acids. the deduced adi protein sequence has a calculated molecular weight of 46.5 ...200919280262
growth condition and bacterial community for maximum hydrolysis of suspended organic materials in anaerobic digestion of food waste-recycling wastewater.this paper reports the effects of changing ph (5-7) and temperature (t, 40-60 degrees c) on the efficiencies of bacterial hydrolysis of suspended organic matter (som) in wastewater from food waste recycling (fwr) and the changes in the bacterial community responsible for this hydrolysis. maximum hydrolysis efficiency (i.e., 50.5% reduction of volatile suspended solids) was predicted to occur at ph 5.7 and t = 44.5 degrees c. changes in short-chain volatile organic acid profiles and in acidogenic ...201019894044
characterization of copper bioreduction and biosorption by a highly copper resistant bacterium isolated from copper-contaminated vineyard soil.copper is an essential but toxic heavy metal that negatively impacts living systems at high concentration. this study presents factors affecting copper bioremoval (bioreduction and biosorption) by a highly copper resistant monoculture of pseudomonas sp. na and copper bioremoval from soil. seven bacteria resistant to high concentration of cu(ii) were isolated from enrichment cultures of vineyard soils and mining wastes. culture parameters influencing copper bioreduction and biosorption by one mon ...201020117823
directed evolution of an antitumor drug (arginine deiminase ppadi) for increased activity at physiological ph.arginine deiminase (adi; ec has been studied as a potential antitumor drug for the treatment of arginine-auxotrophic tumors, such as hepatocellular carcinomas (hccs) and melanomas. studies with human lymphatic leukemia cell lines confirmed that adi is an antiangiogenic agent for treating leukemia. the main limitation of adi from pseudomonas plecoglossicida (ppadi) lies in its ph-dependent activity profile, its ph optimum is at 6.5. a ph shift from 6.5 to 7.5 results in an approximately ...201020157910
exploring characteristics of bioelectricity generation and dye decolorization of mixed and pure bacterial cultures from wine-bearing wastewater treatment.this study uncovered microbial characteristics of bioelectricity generation and dye decolorization in single-chamber microbial fuel cells (mfcs) using activated sludge for wine-containing wastewater treatment. phylogenetic tree analysis on 16s rrna gene fragments indicated that the predominant strains on anodic biofilm in acclimatized mfcs were gamma-proteobacteria aeromonas punctata niu-p9, pseudomonas plecoglossicida niu-y3, pseudomonas koreensis niu-x8, acinetobacter junii niu-y8, stenotropho ...201120859654
a potential antitumor drug (arginine deiminase) reengineered for efficient operation under physiological conditions.arginine deiminase (adi, ec is a potential antitumor drug for the treatment of arginine-auxotrophic tumors such as hepatocellular carcinomas (hccs) and melanomas, and studies on human lymphatic leukemia cell lines have confirmed that adi has antiangiogenic activity. recent studies showed that a combination of taxane and adi-peg20, which induces caspase-independent apoptosis, is more effective than taxane monotherapy for prostate cancer. the main limitation of adi from pseudomonas plecog ...201020954230
rapid evolution of arginine deiminase for improved anti-tumor activity.arginine deiminase (adi), an arginine-degrading enzyme, has been studied as a potential anti-cancer agent for inhibiting arginine-auxotrophic tumors, such as melanomas and hepatocellular carcinomas. based on our preliminary results, it was noticed that the optimum ph of adi from pseudomonas plecoglossicida (ppadi) was 6.0, and less than 10% of the activity was retained at ph 7.4 (ph of human plasma). additionally, the k(m) value for wild-type adi (wt-adi) was 2.88 mm (ph 6.0), which is over 20 t ...201121221572
differential habitat use and niche partitioning by pseudomonas species in human homes.many species of pseudomonas have the ability to use a variety of resources and habitats, and as a result pseudomonas are often characterized as having broad fundamental niches. we questioned whether actual habitat use by pseudomonas species is equally broad. to do this, we sampled extensively to describe the biogeography of pseudomonas within the human home, which presents a wide variety of habitats for microbes that live in close proximity to humans but are not part of the human flora, and for ...201121503776
[isolation of phenol-degrading bacteria from coking wastewater and their degradation gene].phenol and phenolic compounds are main pollutants in wastewater of coking factories. to identify the bacteria responsible for phenol removal in the activated sludge of a coking factory, we isolated bacteria from the sludge directly or after enrichment. from two samples from the aerobic and anaerobic pools, 28 strains belonging to 28 species of 20 genera were obtained after identification with box-pcr and further 16s rdna sequence analyses. most of them belonged to beta- and gamma-proteobacteria, ...201121528584
characterization of a serine hydroxymethyltransferase for l-serine enzymatic production from pseudomonas plecoglossicida.pseudomonas plecoglossicida, a bacterium strain that exhibits high serine hydroxymethyltransferase (shmt) activity, was isolated from the seawater. a full-length glya encoding shmt was obtained by a modified thermal asymmetric interlaced-pcr (tril-pcr), which consisted of 1,254 bp, encoded a 417 amino acid polypeptide, and shared the highest identity (75 %) with a glya gene from acinetobacter radioresistens cmc-1. recombinant glya gene was expressed in escherichia coli bl21 (de3) and purified by ...201323913024
complete genome sequence analysis of two pseudomonas plecoglossicida phages, potential therapeutic agents.pseudomonas plecoglossicida is a lethal pathogen of ayu (plecoglossus altivelis) in japan and is responsible for substantial economic costs to ayu culture. previously, we demonstrated the efficacy of phage therapy against p. plecoglossicida infection using two lytic phages (pppw-3 and pppw-4) (s. c. park, i. shimamura, m. fukunaga, k. mori, and t. nakai, appl environ microbiol 66:1416-1422, 2000,; s. c. park and t. nakai, dis aquat org 53:33-39, ...201525416766
degradation of polynuclear aromatic hydrocarbons by two strains of pseudomonas.the goal of this investigation was to isolate competent polynuclear aromatic hydrocarbons degraders that can utilize polynuclear aromatic hydrocarbons of former industrial sites at mcdoel switchyard in bloomington, indiana. using conventional enrichment method based on soil slurry, we isolated, screened and purified two bacterial species strains pb1 and pb2. applying the ribotyping technique using the 16s rrna gene analysis, the strains were assigned to the genus pseudomonas (pseudomonas plecogl ...201627245129
isolation of bacterial strains able to degrade biphenyl, diphenyl ether and the heat transfer fluid used in thermo-solar plants.thermo-solar plants use eutectic mixtures of diphenyl ether (de) and biphenyl (bp) as heat transfer fluid (htf). potential losses of htf may contaminate soils and bioremediation is an attractive tool for its treatment. de- or bp-degrading bacteria are known, but up to now bacteria able to degrade htf mixture have not been described. here, five bacterial strains which are able to grow with htf or its separate components de and bp as sole carbon sources have been isolated, either from soils expose ...201727884748
bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology.bacteriophages have been proposed as an alternative to antibiotic usage and several studies on their application in aquaculture have been reported. this review highlights progress to date on phage therapies for the following fish and shellfish diseases and associated pathogens: hemorrhagic septicemia (aeromonas hydrophila) in loaches, furunculosis (aeromonas salmonicida) in trout and salmon, edwardsiellosis (edwardsiella tarda) in eel, columnaris disease (flavobacterium columnare) in catfish, ra ...201726713223
biodegradation of nicotine by a novel nicotine-degrading bacterium, pseudomonas plecoglossicida tnd35 and its new biotransformation intermediates.tobacco wastes that contain nicotine alkaloids are harmful to human health and the environment. in the investigation, a novel nicotine-biodegrading bacterium tnd35 was isolated and identified as pseudomonas plecoglossicida on the basis of phenotypic, biochemical characteristics and 16s rrna sequence homology. we have studied the nicotine biodegradation potential of strain tnd35 by detecting the intermediate metabolites using an array of approaches such as hplc, gc-ms, nmr and ft-ir. biotransform ...201423604517
plant growth-promotion and biofortification of chickpea and pigeonpea through inoculation of biocontrol potential bacteria, isolated from organic strains of bacteria [pseudomonas plecoglossicida sri-156, brevibacterium antiquum sri-158, bacillus altitudinis sri-178, enterobacter ludwigii sri-211, e. ludwigii sri-229, acinetobacter tandoii sri-305 and pseudomonas monteilii sri-360; demonstrated previously for control of charcoal rot disease in sorghum and plant growth-promotion (pgp) in rice] were evaluated for their pgp and biofortification traits in chickpea and pigeonpea under field conditions. when treated on seed, the seven sele ...201627833841
evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by macrophomina phaseolina (tassi) goid.a total of 360 bacteria, isolated from the rhizospheres of a system of rice intensification (sri) fields, were characterized for the production of siderophore, fluorescence, indole acetic acid (iaa), hydrocyanic acid (hcn) and solubilization of phosphorus. of them, seven most promising isolates (sri-156, -158, -178, -211, -229, -305 and -360) were screened for their antagonistic potential against macrophomina phaseolina (causes charcoal rot in sorghum) by dual culture assay, blotter paper assay ...201125187130
evaluating the potential of immobilized bacterial consortium for black liquor biodegradation.two indigenous bacterial strains, bacillus megaterium etlb-1 (accession no. kc767548) and pseudomonas plecoglossicida etlb-3 (accession no. kc767547), isolated from soil contaminated with paper mill effluent, were co-immobilized on corncob cubes to investigate their biodegradation potential against black liquor (bl). results exhibit conspicuous reduction in color and lignin of bl upto 913.46 co-pt and 531.45 mg l(-1), respectively. reduction in chlorophenols up to 12 mg l(-1) was recorded with h ...201525433900
characterization of biosurfactants from indigenous soil bacteria recovered from oil contaminated sites.three bacterial isolates (g1, g2 and g3) characterized as pseudomonas plecoglossicida, lysinibacillus fusiformis and bacillus safensis were recovered from contaminated soil of oil refinery. these bacterial isolates produced biosurfactants in msm medium in stationary phase. biosurfactants were characterized on the basis of their emulsifying properties with petrol, diesel, mobil oil and petrol engine oil. reduction in surface tension (below 40 mn m(-1)) and blood hemolysis were also included in bi ...201526521551
phosphate solubilizing rhizobacteria from an organic farm and their influence on the growth and yield of maize (zea mays l.).organic farming is gaining popularity all over the world as it avoids the use of synthetic chemicals. plant production in organic farming mainly depends on nutrient release as a function of mineralization processes in soils. in the present study, efficient phosphate mineralizing bacteria were isolated and their efficacy tested in plant mineral uptake and soil fertility of an organic field. amongst 12 p-solubilizing bacteria (psb) isolated from an organic field, two isolates were selected for fie ...201324005179
identification and characterization of a phospholipase a1 activity type three secreted protein, pp_exou from pseudomonas plecoglossicida nb2011, the causative agent of visceral granulomas disease in large yellow croaker (larimichthys crocea).pseudomonas plecoglossicida nb2011, the causative agent of visceral granulomas disease in farmed larimichthys crocea in china, encodes a predicted type three effector pp_exou, a homolog of the cytotoxin exou of pseudomonas aeruginosa. in this study, secretion of pp_exou was tested in various broth, the protein was expressed with the pet30a prokaryotic system, the phospholipase a (pla) activity of the recombinant protein was determined with fluorogenic phospholipid substrates, fusion expression w ...201627734506
profenofos insecticide degradation by novel microbial consortium and isolates enriched from contaminated chili farm soil.profenofos (pf) is one of the heavily used organophosphorus pesticides (opps) of which its contamination is ubiquitous in an agricultural area. this study aims to acquire and characterize pf-degrading bacterial cultures from contaminated soil. opp degradation by the novel isolates was then investigated. the experiment was performed at the initial pf concentration of 20 mg/l. the result showed that the enriched consortium comprised three predominant pf-degrading strains designated as pf1, pf2, an ...201525065481
zearalenone degradation by two pseudomonas strains from soil.this study describes the screening of soil bacteria for their capability to degrade zearalenone (zen), employing an enrichment technique in which zen is used as the sole carbon source. two isolates that were able to degrade zen belonged to the genus pseudomonas according to biochemical characterization and 16s rrna gene sequence and were named as pseudomonas alcaliphila th-c1 and pseudomonas plecoglossicida th-l1, respectively. the results showed that the degradation rates of p. alcaliphila th-c ...201424879510
a simple strategy for investigating the diversity and hydrocarbon degradation abilities of cultivable bacteria from contaminated soil.the use of indigenous bacterial strains is a valuable bioremediation strategy for cleaning the environment from hydrocarbon pollutants. the isolation and selection of hydrocarbon-degrading bacteria is therefore crucial for obtaining the most promising strains for site decontamination. two different media, a minimal medium supplemented with a mixture of polycyclic aromatic hydrocarbons and a ms medium supplemented with triphenyltetrazolium chloride, were used for the isolation of bacterial strain ...201323386320
pseudomonas guariconensis sp. nov., isolated from rhizospheric soil.we isolated a bacterial strain designated pcavu11(t) in the course of a study of phosphate-solubilizing bacteria occurring in rhizospheric soil of vigna unguiculata (l.) walp. in guárico state, venezuela. the 16s rrna gene sequence had 99.2 % sequence similarity with respect to the most closely related species, pseudomonas taiwanensis, and 99.1 % with respect to pseudomonas entomophila, pseudomonas plecoglossicida and pseudomonas monteilii, on the basis of which pcavu11(t) was classified as repr ...201323847284
isolation and characteristics of sulfentrazone-degrading bacteria.this study aimed to isolate and characterize bacteria able to use sulfentrazone in the commercial formulation as their sole carbon source. the isolation of the potential sulfentrazone-degrading bacteria was made from soil samples with a recent history of herbicide application and from isolates identified through rdna sequencing. subsequently, we assessed the growth of the isolates and their sulfentrazone degradation ability using high-performance liquid chromatography. twenty-six potential sulfe ...201727820678
impact of plant growth promoting pseudomonas monteilii psf84 and pseudomonas plecoglossicida psf610 on metal uptake and production of secondary metabolite (monoterpenes) by rose-scented geranium (pelargonium graveolens cv. bourbon) grown on tannery sludge amended soil.bacterial strains psf84 and psf610 were isolated from tannery sludge polluted soil, jajmau, kanpur, india. 16s rrna gene sequence and phylogenetic analysis confirmed the taxonomic affiliation of psf84 as pseudomonas monteilii and psf610 as pseudomonas plecoglossicida. a greenhouse study was carried out with rose-scented geranium (pelargonium graveolenscv. bourbon) grown in soil treated with tannery sludge in different proportions viz. soil: sludge ratio of 100:0, 25:75, 50:50, 75:25 and 0:100 to ...201425194330
pseudomonas hunanensis sp. nov., isolated from soil subjected to long-term manganese pollution.a gram-negative, polar flagella, rod-shaped bacterium lv (t) was isolated from a soil sample subjected to long-term manganese pollution in hunan province, china. cells grow optimally on luria-bertani agar medium at 30 °c in the presence of 0-5.0 % (w/v) nacl and ph 7-8. 16s rrna gene sequence analysis revealed that strain lv (t) belonged to the genus pseudomonas, with sequence similarity values of 98.6, 98.2, 98.7, and 97.3 % to pseudomonas monteilii bcrc 17520 (t) , pseudomonas putida bcrc 1045 ...201424562759
reutilization of waste scrap tyre as the immobilization matrix for the enhanced bioremoval of a monoaromatic hydrocarbons, methyl tert-butyl ether, and chlorinated ethenes mixture from water.btex (benzene, toluene, ethylbenzene, ortho-, meta-, and para-xylenes), methyl tert-butyl ether (mtbe), cis-1,2-dichloroethylene (cis-dce), and trichloroethylene (tce) are among the major soil and groundwater contaminants frequently co-existing, as a result of their widespread uses. pseudomonas plecoglossicida was immobilized on waste scrap tyre to remove these contaminants mixture from synthetic contaminated water. the microbial activity was enhanced in the immobilized system, shown by the high ...201728109662
temperature-regulated expression of type vi secretion systems in fish pathogen pseudomonas plecoglossicida revealed by comparative secretome analysis.pseudomonas plecoglossicida is a facultative fish pathogen. recent studies showed that p. plecoglossicida infection in fish was associated with temperature. the aim of this study was to compare the secretomes of p. plecoglossicida cultured in vitro at representative temperatures for pathogenic (20°c) and less pathogenic (30°c) phenotypes. thirteen proteins in the culture supernatants of p. plecoglossicida showed significant difference in abundance at 20 vs. 30°c. four proteins were strongly incr ...201627974393
impact of plant growth-promoting rhizobacteria on root colonization potential and life cycle of rhizophagus irregularis following co-entrapment into alginate beads.this study aimed at evaluating the impact of seven plant growth-promoting rhizobacteria (pgpr) on root colonization and life cycle of rhizophagus irregularis mucl 41833 when co-entrapped in alginate beads.201727864849
multivariate optimization for the simultaneous bioremoval of btex and chlorinated aliphatic hydrocarbons by pseudomonas plecoglossicida.this study aimed to evaluate the effects of some major parameters on the cometabolic removal of cis-1,2-dichloroethylene (cis-dce) and trichloroethylene (tce), mixed with benzene, toluene, ethylbenzene, and xylenes, by an indigenous bacterial isolate pseudomonas plecoglossicida. such statistical methodologies as hierarchical cluster analysis heat map and principal component analysis were applied to better evaluate the effects of major parameters (soil ph, temperature, moisture, and cis-dce/tce c ...201727631686
isolation and characterization of a bacterial strain that degrades cis-dichloroethenein the absence of aromatic inducers.bacteria capable of degrading cis-dichloroethene (cdce) were screened from cdce-contaminated soil, and ykd221, a bacterial strain that exhibited a higher growth on minimal salt agar plates in the presence of cdce than in the absence of cdce, were isolated. phylogenetic studies of the 16s rrna as well as gyrb, rpod, and reca in ykd221 indicated that this strain is closely related to the type strains of pseudomonas plecoglossicida, monteilii, and putida. an average nucleotide identity analysis ind ...201627211831
draft genome sequence of a novel nicotine-degrading bacterium, pseudomonas plecoglossicida tnd35.pseudomonas plecoglossicida tnd35 is a potent nicotine-degrading bacterium. the draft genome sequence of strain tnd35 contains 6,209,227 bp, 5,511 coding genes, and a g+c content of 62.3%. it encompasses genes related to catabolism of nicotine, n-heterocyclic aromatic compounds, heavy metal degradation, and butanol biosynthesis.201526184952
hydrophobic mutagenesis and semi-rational engineering of arginine deiminase for markedly enhanced stability and catalytic efficiency.due to its systemic arginine degradation, arginine deiminase (adi) has attracted attentions as an anti-tumor drug. its low activity at physiological conditions among other limitations has necessitated its engineering for improved properties. the present study describes the hydrophobic mutagenesis and semi-rational engineering of adi from pseudomonas plecoglossicida (ppadi). using an improved adi variant m13 (d38h/a128t/e296k/h404r/i410l) as parent, site saturation mutagenesis at position 162 res ...201526041055
effect of toluene concentration and hydrogen peroxide on pseudomonas plecoglossicida cometabolizing mixture of cis-dce and tce in soil indigenous pseudomonas sp., isolated from the regional contaminated soil and identified as p. plecoglossicida, was evaluated for its aerobic cometabolic removal of cis-1,2-dichloroethylene (cis-dce) and trichloroethylene (tce) using toluene as growth substrate in a laboratory-scale soil slurry. the aerobic simultaneous bioremoval of the cis-dce/tce/toluene mixture was studied under different conditions. results showed that an increase in toluene concentration level from 300 to 900 mg/kg prolo ...201525963576
combined removal of a btex, tce, and cis-dce mixture using pseudomonas sp. immobilized on scrap tyres.the simultaneous aerobic removal of a mixture of benzene, toluene, ethylbenzene, and o,m,p-xylene (btex); cis-dichloroethylene (cis-dce); and trichloroethylene (tce) from the artificially contaminated water using an indigenous bacterial isolate identified as pseudomonas plecoglossicida immobilized on waste scrap tyres was investigated. suspended and immobilized conditions were compared for the removal of these volatile organic compounds. for the immobilized system, toluene, benzene, and ethylben ...201525956516
complete genome sequence of the cyclohexylamine-degrading pseudomonas plecoglossicida nyz12.pseudomonas plecoglossicida nyz12 (cctcc ab 2015057), a gram-negative bacterium isolated from soil, has the ability to degrade cyclohexylamine. the complete genome sequence of this strain (6,233,254bp of chromosome length) is presented, with information about the genes of characteristic enzymes responsible for cyclohexylamine oxidation to cyclohexanone and the integrated gene cluster for the metabolic pathway of cyclohexanone oxidation to adipate.201525701176
a competitive flow cytometry screening system for directed evolution of therapeutic enzyme.a ligand-mediated egfp-expression system (limex) was developed as a novel flow cytometry based screening platform that relies on a competitive conversion/binding of arginine between arginine deiminase and arginine repressor. unlike product-driven detection systems, the competitive screening platform allows to evolve enzymes toward efficient operation at low substrate concentrations under physiological conditions. the principle of limex was validated by evolving arginine deiminase (adi, an antica ...201525658761
pegylation and pharmacological characterization of a potential anti-tumor drug, an engineered arginine deiminase originated from pseudomonas plecoglossicida.arginine deiminase (adi) has been studied as a potential anti-cancer agent for arginine-auxotrophic tumors. pegylation is one of the best methods to formulate a bioconjugated protein with extended physical stability and reduced immunogenicity. here, pegylation and pharmacological properties of an engineered adi originated from pseudomonas plecoglossicida were studied. among polyethylene glycol (peg) reagents with succinimidyl ester groups varying in size and linkers, three pegylated products wit ...201525462857
visceral granulomas in farmed large yellow croaker, larimichthys crocea (richardson), caused by a bacterial pathogen, pseudomonas enzootic disease characterized by granulomas in internal organs occurred in cage-farmed large yellow croaker, larimichthys crocea (richardson), in april and november 2010, in ningbo, zhejiang province. one bacterial strain, named xsdhy-p, was isolated from the diseased fish and identified by biochemical characterization, fatty acid methyl ester (fame) analysis and multilocus sequence analysis (mlsa). according to the results obtained from the biochemical tests, fame analysis and phylogenetic ...201425254273
directed arginine deiminase evolution for efficient inhibition of arginine-auxotrophic melanomas.arginine deiminase (adi) is a therapeutic protein for cancer therapy of arginine-auxotrophic tumors. however, adi's application as anticancer drug is hampered by its low activity for arginine under physiological conditions mainly due to its high "k m" (s₀.₅) values which are often 1 magnitude higher than the arginine concentration in blood (0.10-0.12 mm arginine in human plasma). previous evolution campaigns were directed by us with the aim of boosting activity of ppadi (adi from pseudomonas ple ...201525104032
protein engineering of the antitumor enzyme ppadi for improved thermal resistance.arginine deiminase (adi, ec is a potential antitumor drug for the treatment of arginine-auxotrophic tumors such as hepatocellular carcinomas (hccs) and melanomas. studies in human lymphatic leukemia cell lines have confirmed the anti-angiogenic activity of adi. activity and thermal resistance limit the efficacy of adi in treatment of auxotrophic tumors. previously, we reengineered adi from pseudomonas plecoglossicida (ppadi) for improved activity under physiological conditions (37 °c, p ...201424375953
draft genome sequence of pseudomonas plecoglossicida strain nb2011, the causative agent of white nodules in large yellow croaker (larimichthys crocea).we describe the draft genome sequence of pseudomonas plecoglossicida strain nb2011, the causative agent of white nodules in cultured large yellow croaker (larimichthys crocea) in china. the draft genome sequence of the bacterium consists of 5.41 million bp, with a g+c content of 62.8%. a total of 4,952 genes were identified.201323929479
biosorption of cd(ii) from aqueous solution by pseudomonas plecoglossicida: kinetics and our present study, we investigated the mechanism of cd(ii) biosorption from aqueous solution by pseudomonas plecoglossicida using different instrumental techniques. the adsorption kinetics fitted well with the pseudo second-order model, suggesting that the cd(ii) adsorption by p. plecoglossicida consisted of a chemisorption and a physisorption process. compared with the dead p. plecoglossicida cells, the live cells demonstrated the same adsorption capacity of cd(ii). scanning electron microsc ...201222706778
genotypic and phenotypic diversity of pgpr fluorescent pseudomonads isolated from the rhizosphere of sugarcane (saccharum officinarum l.).the genetic diversity of plant growth-promoting rhizobacterial (pgpr) fluorescent pseudomonads associated with the sugarcane (saccharum officinarum l.) rhizosphere was analyzed. selected isolates were screened for plant growthpromoting properties including production of indole acetic acid, phosphate solubilization, denitrification ability, and production of antifungal metabolites. furthermore, 16s rdna sequence analysis was performed to identify and differentiate these isolates. based on 16s rdn ...201222297215
genome-wide identification and characterization of genes encoding cyclohexylamine degradation in a novel cyclohexylamine-degrading bacterial strain of pseudomonas plecoglossicida nyz12.the gram-negative strain of pseudomonas plecoglossicida nyz12 isolated from soil has the ability to degrade cyclohexylamine (cham). the genes encoding cham degradation by gram-negative bacteria, however, have not been reported previously. in this study, orfs predicted to encode cham degradation by nyz12 were identified by bioinformatics analysis. differential expression of the proposed orfs was analyzed via rna-seq and quantitative reverse transcription-pcr (qrt-pcr), using rna extracted from ny ...201728472672
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