| nucleotide sequences of membrane-bound hydrogenase gene in alcaligenes hydrogenophilus. | the nucleotide sequences of membrane-bound hydrogenase small (hups) and large (hupl) subunit genes of hydrogen bacterium alcaligenes hydrogenophilus were determined. the hups and hupl genes encoded polypeptides of 363 and 619 amino acids, respectively. the hups was located upstream of hupl with 35bp of intergenic region. the consensus ribosome-binding sequences were identified upstream of the start codons of hups and hupl. amino acid sequence of hups is very similar to that of rhodobacter capsul ... | 1992 | 1294332 |
| immunological comparison of subunits isolated from various hydrogenases of aerobic hydrogen bacteria. | polyclonal, monospecific antibodies were produced against the two subunits (mr 62,000, and mr 31,000), isolated from the membrane-bound hydrogenase of alcaligenes eutrophus h16. the antibodies (igg fractions) were purified from crude sera by protein a-sepharose cl-4b chromatography. by double immunodiffusion assays and tandem-crossed immunoelectrophoresis the large and the small subunit were demonstrated not to be immunologically related. immunological comparison of these subunits with the four ... | 1989 | 2493816 |
| 13c-nmr study of autotrophic co2 fixation in thermoproteus neutrophilus. | the pathway of autotrophic co2 fixation has been investigated in the extremely thermophilic sulfur-respiring anaerobic archaebacterium thermoproteus neutrophilus. [1,4-13c2]succinate was used as a tracer since this compound was incorporated in small amounts virtually into all cell compounds without affecting the organism's ability to synthesize all cell constituents from co2. three representative amino acids, glutamate, aspartate and alanine were isolated from cells after growth for several gene ... | 1989 | 2506014 |
| cloning of pmol28-encoded nickel resistance genes and expression of the genes in alcaligenes eutrophus and pseudomonas spp. | the 163-kilobase-pair (kb) plasmid pmol28, which determines inducible resistance to nickel, cobalt, chromate, and mercury salts in its native host alcaligenes eutrophus ch34, was transferred to a derivative of a. eutrophus h16 and subjected to cloning procedures. after tn5 transposon mutagenesis, restriction endonuclease analysis, and dna-dna hybridization, two dna fragments, a 9.5-kb kpni fragment and a 13.5-kb hindiii fragment (hki), were isolated. hki contained ek1, the kpni fragment, as a su ... | 1989 | 2549012 |
| genetics of hydrogenase from aerobic lithoautotrophic bacteria. | aerobic facultatively autotrophic hydrogen bacteria are distinguished on the basis of their hydrogen-oxidizing enzyme system (hox). the major group, represented by paracoccus denitrificans and pseudomonas facilis, contains a membrane-bound, electron transport-coupled protein. species of nocardia are characterized by the possession of a cytoplasmic nad-dependent hydrogenase. both enzymes are present in strains of alcaligenes. all hydrogenases from lithoautotrophs are h2-consuming nickel-iron-sulf ... | 1986 | 3089306 |
| transformation of tetrachloromethane to dichloromethane and carbon dioxide by acetobacterium woodii. | five anaerobic bacteria were tested for their abilities to transform tetrachloromethane so that information about enzymes involved in reductive dehalogenations of polychloromethanes could be obtained. cultures of the sulfate reducer desulfobacterium autotrophicum transformed some 80 microm tetrachloromethane to trichloromethane and a small amount of dichloromethane in 18 days under conditions of heterotrophic growth. the acetogens acetobacterium woodii and clostridium thermoaceticum in fructose- ... | 1988 | 3145712 |
| conjugal transfer of hydrogen-oxidizing ability of alcaligenes hydrogenophilus to pseudomonas oxalaticus. | conjugal transfer of hydrogen-oxidizing ability (hox) of the hydrogen bacterium alcaligenes hydrogenophilus was examined. intraspecific cross of plasmid phg21-a that encodes hydrogenases that mediate hydrogen oxidation was most frequent at 25 c; the optimal temperature for growth was 30 c. the plasmid could be transferred from a. hydrogenophilus to pseudomonas oxalaticus ox1 and ox4, and the resulting strains gained the capacity for autotrophic growth with h2 and co2. plasmid phg21-a was maintai ... | 1986 | 3521600 |
| isolation of hydrogen-oxidation gene from alcaligenes hydrogenophilus and its expression in pseudomonas oxalaticus. | a gene bank of a megaplasmid encoding the hydrogen-oxidizing enzyme system (hox) in alcaligenes hydrogenophilus was constructed using a broad host range cosmid vector pvk102, and established in escherichia coli. hybrid cosmids containing hox genes were identified by transferring the bank into pseudomonas oxalaticus ox1 and screening colonies for the ability of h2-dependent autotrophic growth. about 800 colonies were formed under autotrophic conditions. one of the hox+ transconjugants was isolate ... | 1986 | 3521601 |
| isolation and characterization of megaplasmid dna from lithoautotrophic bacteria. | a method is described for the preparative isolation of megaplasmids ranging in size from 340 to 700 kb. these plasmids were isolated from chemolithoautotrophic bacteria including the species alcaligenes, pseudomonas, and paracoccus. the procedure was based on alkaline sodium dodecyl sulfate lysis of the cells, followed by heat treatment, salt precipitation, several phenol extractions, dialysis steps, and proteinase and rnase treatment. the various parameters were evaluated and controlled. hydrog ... | 1984 | 6098903 |
| expression of hydrogenase in alcaligenes spp. is altered by interspecific plasmid exchange. | alcaligenes hydrogenophilus was found to contain a soluble and a particulate hydrogenase whose control and structure differed in part from that in alcaligenes eutrophus. one of at least two plasmids indigenous to a. hydrogenophilus determines hydrogenase genes (hox). the interspecific exchange of hox-encoding plasmids generated transconjugants which expressed the structural and regulatory hox phenotype of the donor. | 1984 | 6370961 |
| [conjugal transfer of chemolithoautotrophically growing ability from hydrogen-oxidizing bacterium alcaligenes hydrogenophilus to useful material-producing bacteria]. | the plasmid genes encoding the ability to grow chemolithoautotrophically with h2 and co2 from a h2-oxidizing bacterium alcaligenes hydrogenophilus were in vivo cloned using the broad host range inc p1 r-plasmid r68.45. the genes of h2-oxidation enzymes were expressed by a novel alternative sigma 54-like factor encoded on the chromosome. the sigma 54-like factor was also in vivo cloned using r68.45. both r68.45-primes, one carrying plasmid genes and the other carrying chromosome genes, were in vi ... | 1994 | 8169770 |
| demethylation of dimethylsulfoniopropionate to 3-s-methylmercaptopropionate by marine sulfate-reducing bacteria. | the initial step in the anaerobic degradation of the algal osmolyte dimethylsulfoniopropionate (dmsp) in anoxic marine sediments involves either a cleavage to dimethylsulfide and acrylate or a demethylation to 3-s-methylmercaptopropionate. thus far, only one anaerobic bacterial strain has been shown to carry out the demethylation, namely, desulfobacterium sp. strain pm4. the aims of the present work were to study how common this property is among certain groups of anaerobic bacteria and to obtai ... | 1996 | 8899985 |
| a hydrogen-sensing system in transcriptional regulation of hydrogenase gene expression in alcaligenes species. | heterologous complementation studies using alcaligenes eutrophus h16 as a recipient identified a hydrogenase-specific regulatory dna region on megaplasmid phg21-a of the related species alcaligenes hydrogenophilus. nucleotide sequence analysis revealed four open reading frames on the subcloned dna, designated hoxa, hoxb, hoxc, and hoxj. the product of hoxa is homologous to a transcriptional activator of the family of two-component regulatory systems present in a number of h2-oxidizing bacteria. ... | 1997 | 9045826 |
| cloning and sequence analysis of the poly (3-hydroxyalkanoic acid)-synthesis genes of pseudomonas acidophila. | pseudomonas acidophila can grow with co2 as a sole carbon source by the possession of a recombinant plasmid that clones genes that confer chemolithoautotrophic growth ability derived from the h2-oxidizing bacterium alcaligenes hydrogenophilus. h2-oxidizing bacteria produce poly(3-hydroxybutyric acid) (phb) from co2, but recombinant p. acidophila can produce the more useful biopolymer poly(3-hydroxyalkanoic acid) (pha). in this study, the pha genes of p. acidophila were cloned and a sequence anal ... | 1998 | 9627389 |
| hydrogenophilus thermoluteolus gen. nov., sp. nov., a thermophilic, facultatively chemolithoautotrophic, hydrogen-oxidizing bacterium. | the taxonomic positions of 'pseudomonas hydrogenothermophila' strain th-1 and 'flavobacterium autothermophilum' strain th-4 were studied by 16s rdna sequencing. these organisms are gram-negative, strictly aerobic, thermophilic, facultatively chemolithoautotrophic hydrogen-oxidizing rods and have a dna g + c content of 63-65 mol%. the major isoprenoid quinone is ubiquinone-8 and 3-hydroxy decanoic acid (3-oh c10:0) is the major 3-hydroxy cellular fatty acid. a phylogenetic analysis based on 16s r ... | 1999 | 10319503 |
| the cbbq genes, located downstream of the form i and form ii rubisco genes, affect the activity of both rubiscos. | hydrogenovibrio marinus strain mh-110, an obligately lithoautotrophic hydrogen-oxidizing bacterium, possesses three sets of the genes for ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco): namely, two form i type (cbbls-1 and cbbls-2) and one form ii type (cbbm) enzymes. the cbbq-m gene was located downstream of cbbm, and showed high similarity to other cbbq genes and nirq/norq genes in denitrifying bacteria. phylogenetic analysis of cbbq and nirq/norq indicated that cbbq-m from hv. mari ... | 1999 | 10548510 |
| structure of ribulose 1,5-bisphosphate carboxylase/oxygenase gene cluster from a thermophilic hydrogen-oxidizing bacterium, hydrogenophilus thermoluteolus, and phylogeny of the fructose 1,6-bisphosphate aldolase encoded by cbba in the cluster. | four genes, cbbo, cbby, cbba, and the pyruvate kinase gene (pyk), were found downstream of ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) genes, cbbls, from a thermophilic hydrogen-oxidizing bacterium, hydrogenophilus thermoluteolus (formerly pseudomonas hydrogenothermophila). cbbo was similar to nord in the denitrification gene cluster, and cbby was similar to cbby from other autotrophic bacteria. cbba encoded fructose 1,6-bisphosphate aldolase (fbp aldolase); however, cbba was littl ... | 2000 | 10705449 |
| hydrogenophilus hirschii sp. nov., a novel thermophilic hydrogen-oxidizing beta-proteobacterium isolated from yellowstone national park. | a novel thermophilic hydrogen-oxidizing bacterium, hydrogenophilus hirschii yel5at (= dsm 11420t = jcm 10831t) has been isolated from the angel terrace spring, yellowstone national park. the isolate was rod-shaped (1.0-1.5 x 0.8 microm) with a polarly inserted flagellum. cells grew chemolithoautotrophically under an atmosphere of h2 and co2 (80:20) in the presence of low concentrations of o2 (optimum 2.5%). organotrophic growth occurred on complex organic substrates such as yeast extract and pep ... | 2001 | 11321094 |
| geobacter hydrogenophilus, geobacter chapellei and geobacter grbiciae, three new, strictly anaerobic, dissimilatory fe(iii)-reducers. | recent studies on the diversity and ubiquity of fe(iii)-reducing organisms in different environments led to the isolation and identification of four new dissimilatory fe(iii)-reducers (strains h-2t, 172t, tacp-2t and tacp-5). all four isolates are non-motile, gram-negative, freshwater, mesophilic, strict anaerobes with morphology identical to that of geobacter metallireducens strain gs-15t. analysis of the 16s rrna sequences indicated that the new isolates belong to the genus geobacter, in the d ... | 2001 | 11321104 |
| cbbr, a lysr-type transcriptional regulator from hydrogenophilus thermoluteolus, binds two cbb promoter regions. | the cbbr encoding the lysr-type transcriptional regulator is located downstream of cbblsqoya and this gene is located upstream of cbbfpt in divergent transcription. the two promoter regions with lysr-binding sites are located in the cbbl upstream region and in the cbbr-cbbf intergenic region. electrophoretic mobility shift assays using a cell extract of escherichia coli harboring a plasmid containing cbbr and the dna fragments of promoter regions indicated that cbbr binds in both regions. nadph ... | 2001 | 11430407 |
| hydrogenothermus marinus gen. nov., sp. nov., a novel thermophilic hydrogen-oxidizing bacterium, recognition of calderobacterium hydrogenophilum as a member of the genus hydrogenobacter and proposal of the reclassification of hydrogenobacter acidophilus as hydrogenobaculum acidophilum gen. nov., comb. nov., in the phylum 'hydrogenobacter/aquifex'. | a novel thermophilic, hydrogen-oxidizing bacterium, vm1t, has been isolated from a marine hydrothermal area of vulcano island, italy. cells of the strain were gram-negative rods, 2-4 microm long and 1-1.5 microm wide with four to seven monopolarly inserted flagella. cells grew chemolithoautotrophically under an atmosphere of h2/co2 (80:20) in the presence of low concentrations of o2 (optimum 1-2%). carbohydrates and peptide substrates were not utilized, neither for energy generation nor as a sou ... | 2001 | 11594618 |
| atp binding and hydrolysis and autophosphorylation of cbbq encoded by the gene located downstream of rubisco genes. | cbbq is encoded by the gene located downstream of ribulose 1,5-bisphosphate carboxylase/oxygenase genes (cbbls) in the thermophilic hydrogen-oxidizing bacterium, hydrogenophilus thermoluteolus. the protein possesses two nucleotide-binding motifs in its amino acid sequence, and it posttranslationally activates rubisco. we present atp hydrolysis and binding of cbbq. cbbq releases p(i) from atp only in the presence of mg(2+). cbbq interacts with an 2'(3')-o-(2,4,6-trinitrophenyl)adenosine 5'-tripho ... | 2002 | 11820782 |
| genetics and control of co(2) assimilation in the chemoautotroph ralstonia eutropha. | the nutritional versatility of facultative autotrophs requires efficient overall control of their metabolism. most of these organisms are proteobacteria that assimilate co(2) via the highly energy-demanding calvin-benson-bassham reductive pentose-phosphate cycle. the enzymes of the cycle are encoded by cbb genes organized in cbb operons differing in size and composition, although conserved features are apparent. transcription of the operons, which may form regulons, is strictly controlled, being ... | 2002 | 12115053 |
| stable carbon isotope fractionation by sulfate-reducing bacteria. | biogeochemical transformations occurring in the anoxic zones of stratified sedimentary microbial communities can profoundly influence the isotopic and organic signatures preserved in the fossil record. accordingly, we have determined carbon isotope discrimination that is associated with both heterotrophic and lithotrophic growth of pure cultures of sulfate-reducing bacteria (srb). for heterotrophic-growth experiments, substrate consumption was monitored to completion. sealed vessels containing s ... | 2003 | 12732570 |
| tepidiphilus margaritifer gen. nov., sp. nov., isolated from a thermophilic aerobic digester. | a moderately thermophilic bacterium is described, strain n2-214(t), that was isolated from an enrichment culture, growing on caprolactone, obtained from a sample from a water-treatment sludge aerobic digester operating at temperatures around 60 degrees c. the organism was aerobic, gram-negative, oxidase- and catalase-positive, with a polar flagellum, and capable of growth at temperatures as high as 61 degrees c. the major fatty acids of strain n2-214(t) were c(16 : 0), c(18 : 1) and cyclo-c(19 : ... | 2003 | 13130025 |
| effect of growth temperature on cellular fatty acids in sulphate-reducing bacteria. | the effect of growth temperature on the cellular fatty acid composition of sulphate-reducing bacteria (srb) was studied in 12 species belonging to eight genera including psychrophiles and mesophiles. most of these species were of marine origin. the investigated srb with the exception of four desulfobacter species exhibited only a minor increase in the proportion of cis-unsaturated fatty acids (by < or = 5% per 10 degrees c) when the growth temperature was decreased; psychrophiles maintained thei ... | 2003 | 14641586 |
| stable carbon isotope ratios of lipid biomarkers of sulfate-reducing bacteria. | we examined the potential use of natural-abundance stable carbon isotope ratios of lipids for determining substrate usage by sulfate-reducing bacteria (srb). four srb were grown under autotrophic, mixotrophic, or heterotrophic growth conditions, and the delta13c values of their individual fatty acids (fa) were determined. the fa were usually 13c depleted in relation to biomass, with deltadelta13c(fa - biomass) of -4 to -17 per thousand; the greatest depletion occurred during heterotrophic growth ... | 2004 | 14766550 |
| petrobacter succinatimandens gen. nov., sp. nov., a moderately thermophilic, nitrate-reducing bacterium isolated from an australian oil well. | a novel gram-negative, aerobic and moderately thermophilic bacterium, strain 4bon(t), was isolated from a non-water-flooded australian terrestrial oil reservoir. cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. the optimum growth conditions were 55 degrees c, ph 6.9 and 0.5 % nacl. strain 4bon(t) was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or nitrate as termi ... | 2004 | 15143002 |
| description of heterotrophic bacteria occurring in paper mills and paper products. | to isolate aerobic mesophilic bacilli and thermophilic bacteria from different paper mill samples and to evaluate their potential harmfulness. | 2004 | 15546414 |
| microbial community in a geothermal aquifer associated with the subsurface of the great artesian basin, australia. | to investigate the biomass and phylogenetic diversity of the microbial community inhabiting the deep aquifer of the great artesian basin (gab), geothermal groundwater gushing out from the aquifer was sampled and analyzed. microbial cells in the groundwater were stained with acridine orange and directly counted by epifluorescence microscopy. microbial cells were present at a density of 10(8)-10(9) cells per liter of groundwater. archaeal and bacterial small-subunit rrna genes (rdnas) were amplifi ... | 2005 | 15980939 |
| isolation and characterization of co2-fixing hydrogen-oxidizing marine bacteria. | a co2-fixing bacterium, strain yn-1, that can fix co2 under chemoautotrophic conditions but not photoautotrophic conditions was isolated from seawater. identification of the isolate was carried out using biochemical tests and 16s rdna sequence analysis, and its characteristics were investigated. from the results of partial 16s rdna sequence analysis, strain yn-1 showed low identity with previously reported hydrogen-oxidizing bacteria, hydrogenovibrio marinus mh-110 and hydrogenophilus thermolute ... | 2001 | 16233020 |
| desulfobacter psychrotolerans sp. nov., a new psychrotolerant sulfate-reducing bacterium and descriptions of its physiological response to temperature changes. | a psychrotrolerant acetate-oxidizing sulfate-reducing bacterium (strain akvb(t)) was isolated from sediment from the northern part of the north sea with annual temperature fluctuations between 8 and 14 degrees c. of the various substrates tested, strain akvb(t) grew exclusively by the oxidation of acetate coupled to the reduction of sulfate. the cells were motile, thick rods with round ends and grew in dense aggregates. strain akvb(t) grew at temperatures ranging from -3.6 to 26.3 degrees c. opt ... | 2006 | 16328859 |
| molecular signatures in protein sequences that are characteristics of the phylum aquificae. | species of the phylum aquificae are of great interest due to their strict extreme thermophilic growth characteristics. presently, there is no known molecular characteristic which is unique to this group of bacteria. this work describes six conserved inserts and deletions (indels or signature sequences) in four widely distributed proteins that are distinctive features of species from the phylum aquificae. these include three signatures consisting of a 2 aa insert, a 5-6 aa insert and a 6 aa delet ... | 2006 | 16403873 |
| cloning, expression, crystallization and preliminary x-ray characterization of cytochrome c552 from a moderate thermophilic bacterium, hydrogenophilus thermoluteolus. | the amino-acid sequence of cytochrome c552 (ph c552) from a moderately thermophilic bacterium, hydrogenophilus thermoluteolus, was more than 50% identical to that of cytochrome c from an extreme thermophile, hydrogenobacter thermophilus (ht c552), and from a mesophile, pseudomonas aeruginosa (pa c551). the ph c552 gene was overexpressed as a correctly processed holoprotein in the escherichia coli periplasm. the overexpressed ph c552 has been crystallized by vapour diffusion from polyethylene gly ... | 2005 | 16511051 |
| biosynthesis of poly(3-hydroxyalkanoic acid) copolymer from co(inf2) in pseudomonas acidophila through introduction of the dna fragment responsible for chemolithoautotrophic growth of alcaligenes hydrogenophilus. | pseudomonas acidophila is a bacterial strain producing a poly(3-hydroxyalkanoic acid) (pha) copolymer from low-molecular-weight organic compounds such as formate and acetate. the genes responsible for pha production were cloned in cosmid pik7 containing a 14.8-kb hindiii fragment of p. acidophila dna. with the aim of developing a means of producing a pha copolymer from co(inf2), cosmid pik7 was introduced into a polymer-negative mutant of the chemolithoautotrophic bacterium alcaligenes eutrophus ... | 1996 | 16535252 |
| structure of cytochrome c552 from a moderate thermophilic bacterium, hydrogenophilus thermoluteolus: comparative study on the thermostability of cytochrome c. | we have studied the structure-thermostability relationship using cytochromes c from mesophilic and thermophilic bacteria; pseudomonas aeruginosa (pac(551)) growing at 37 degrees c and hydrogenobacter thermophilus (htc(552)) at 72 degrees c and showed that only five residues primarily differentiate their stabilities. for a more comprehensive study, we found hydrogenophilus thermoluteolus (pseudomonas hydrogenothermophila) growing at 52 degrees c and showed the moderate stability of the cytochrome ... | 2006 | 16681384 |
| presence of hydrogenophilus thermoluteolus dna in accretion ice in the subglacial lake vostok, antarctica, assessed using rrs, cbb and hox. | the 3561 m vostok ice core sample originating from the subglacial lake vostok accretion (frozen lake water) ice with sediment inclusions was thoroughly studied by various means to confirm the presence of the thermophile bacterium hydrogenophilus thermoluteolus reported earlier in the 3607 m accretion ice sample. pcr and molecular-phylogenetic analyses performed in two independent laboratories were made using different 16s rrna gene (rrs) targeted primers. as a result, rrs-targeted pcr permitted ... | 2006 | 17107552 |
| microbial diversity and methanogenic potential in a high temperature natural gas field in japan. | microbial diversity and methanogenic potential in formation water samples from a dissolved-in-water type gas field were investigated by using 16s rrna gene libraries and culture-based methods. two formation water samples (of 46 and 53 degrees c in temperature) were obtained from a depth of 700 to 800 m. coenzyme f(420)-autofluorescence indicated that 10(3)-10(4) cells per ml of active methanogens were present, accounting for at least 10% of the total cell count. the 16s rrna gene sequence analys ... | 2007 | 17265161 |
| thiosulfate oxidation by a moderately thermophilic hydrogen-oxidizing bacterium, hydrogenophilus thermoluteolus. | the moderately thermophilic betaproteobacterium, hydrogenophilus thermoluteolus, not only oxidizes hydrogen, the principal electron donor for growth, but also sulfur compounds including thiosulfate, a process enabled by sox genes. a periplasmic extract of h. thermoluteolus showed significant thiosulfate oxidation activity. ten genes apparently involved in thiosulfate oxidation (soxefcdyzaxbh) were found on a 9.7-kb dna fragment of the h. thermoluteolus chromosome. the proteins soxax, which repre ... | 2007 | 17516047 |
| optimization of biomass productivity and substrate utility of a hydrogen bacterium, alcaligenes hydrogenophilus. | optimizations of cell productivity and utility of gaseous substrates were investigated for alcaligenes hydrogenophilus. theoretical analyses for the cell productivity and substrate utility were discussed in the light of experimental data. it was proved from the theoretical analyses that the maximum cell productivity was at the following conditions: d = 0.21 h(-1), r(1) ( = h(2) partial pressure/o(2) partial pressure) = 2.25 and r(2) ( =co(2) partial pressure/o(2) partial pressure) = 0.09, i.e., ... | 1982 | 18546410 |
| cloning and sequence analysis of the poly(3-hydroxyalkanoic acid)-synthesis genes of pseudomonas acidophila. | pseudomonas acidophila can grow with co2 as a sole carbon source by the possession of a recombinant plasmid that clones genes that confer chemolithoautotrophic growth ability derived from the h2-oxidizing bacterium alcaligenes hydrogenophilus. h2-oxidizing bacteria produce poly(3-hydroxybutyric acid) (phb) from co2, but recombinant p. acidophila can produce the more useful biopolymer poly(3-hydroxyalkanoic acid) (pha). in this study, the pha genes of p. acidophila were cloned and a sequence anal ... | 1998 | 18576003 |
| stabilization mechanism of cytochrome c552 from a moderately thermophilic bacterium, hydrogenophilus thermoluteolus. | cytochrome c(552) (ph c(552)) from moderately thermophilic hydrogenophilus thermoluteolus exhibits stability intermediate between those of cytochrome c(552) (ht c(552)) from thermophilic hydrogenobacter thermophilus and cytochrome c(551) (pa c(551)) from mesophilic pseudomonas aeruginosa. to understand the mechanism of stabilization of ph c(552), we introduced mutations into ph c(552) at five sites, which, in ht c(552), are occupied by the amino acids responsible for stability higher than the le ... | 2008 | 18685221 |
| carbon isotope fractionation by sulfate-reducing bacteria using different pathways for the oxidation of acetate. | acetate is a key intermediate in the anaerobic degradation of organic matter. in anoxic environments, available acetate is a competitive substrate for sulfate-reducing bacteria (srb) and methane-producing archaea. little is known about the fractionation of carbon isotopes by sulfate reducers. therefore, we determined carbon isotope compositions in cultures of three acetate-utilizing srb, desulfobacter postgatei, desulfobacter hydrogenophilus, and desulfobacca acetoxidans. we found that these spe ... | 2008 | 19031865 |
| correlation between the stability and redox potential of three homologous cytochromes c from two thermophiles and one mesophile. | the stability of the oxidized and reduced forms of three homologous cytochromes c from two thermophiles and one mesophile was systematically monitored by means of soret absorption measurements in the presence of various concentrations of a denaturant, guanidine thiocyanate, at ph 7.0 at 25 degrees c. thermophilic hydrogenobacter thermophilus cytochrome c(552) was the most stable in both redox states, followed by moderately thermophilic hydrogenophilus thermoluteolus cytochrome c(552), and then m ... | 2009 | 19202290 |
| effects of cysteine introduction into three homologous cytochromes c. | a cysteine residue was systematically introduced into three homologous cytochromes c from hydrogenobacter thermophilus, hydrogenophilus thermoluteolus, and pseudomonas aeruginosa at a conserved position. the h. thermoluteolus variant showed the most decreased thermal stability as compared with the wild type, which might have been due in part to crosslinked polymer formation. the effects of cysteine introduction differed even at the conserved position in these homologous proteins. | 2009 | 19420693 |
| amplified ribosomal dna restriction analysis of free-living bacteria present in the headbox of a canadian paper machine. | the headbox water is the main source of bacterial contamination of paper machines. identification of these bacterial contaminants could be an asset in developing specific control methods. an amplified ribosomal dna restriction analysis (ardra) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a canadian mill in february and july 2006. eight bacterial genera were identified as the main colonizers present in the headbox water. the gen ... | 2009 | 19767853 |
| description of the novel perchlorate-reducing bacteria dechlorobacter hydrogenophilus gen. nov., sp. nov.and propionivibrio militaris, sp. nov. | novel dissimilatory perchlorate-reducing bacteria (dprb) were isolated from enrichments conducted under conditions different from those of all previously described dprb. strain lt-1(t) was enriched using medium buffered at ph 6.6 with 2-(n-morpholino)ethanesulfonic acid (mes) and had only 95% 16s rrna gene identity with its closest relative, azonexus caeni. strain mp(t) was enriched in the cathodic chamber of a perchlorate-reducing bioelectrical reactor (ber) and together with an additional stra ... | 2010 | 19921177 |
| hydrogenophilus islandicus sp. nov., a thermophilic hydrogen-oxidizing bacterium isolated from an icelandic hot spring. | a novel chemolithotrophic bacterium, strain 16c(t), was isolated from a hot spring in graendalur, south-west iceland. cells of this organism were gram-negative, rod-shaped and motile. the isolate was aerobic and capable of chemolithotrophic growth on hydrogen and carbon dioxide, heterotrophic growth on butyrate and several other organic compounds, and mixotrophic growth on butyrate, hydrogen and carbon dioxide. heterotrophic growth was generally enhanced in the presence of yeast extract. autotro ... | 2011 | 20228213 |
| typical methanogenic inhibitors can considerably alter bacterial populations and affect the interaction between fatty acid degraders and homoacetogens. | the effects of two typical methanogenic inhibitors [2-bromoethanesulfonate (bes) and chloroform (chcl(3))] on the bacterial populations were investigated using molecular ecological techniques. terminal restriction fragment length polymorphism analyses (t-rflp) in combination with clone library showed that both the toxicants not only inhibited methanogenic activity but also considerably altered the bacterial community structure. species of low % g + c gram-positive bacteria (clostridiales), high ... | 2010 | 20559824 |
| heterologous synthesis of cytochrome c' by escherichia coli is not dependent on the system i cytochrome c biogenesis machinery. | hydrogenophilus thermoluteolus cytochrome c' (phcp) has typical spectral properties previously observed for other cytochromes c', which comprise ambler's class ii cytochromes c. the phcp protein sequence (135 amino acids) deduced from the cloned gene is the most homologous (55% identity) to that of cytochrome c' from allochromatium vinosum (avcp). these findings indicate that phcp forms a four-helix bundle structure, similar to avcp. strikingly, phcp with a covalently bound heme was heterologous ... | 2011 | 21554540 |
| reverse-transcriptase quantitative pcr method to detect uptake of hydrogen produced from cyanobacteria by alcaligenes hydrogenophilus, an aerobic hydrogen-oxidising bacterium. | hydrogen-oxidising bacteria play a key ecological role in a variety of habitats including the rhizosphere and hot springs. to investigate the possibly of interspecies hydrogen exchange between cyanobacteria and hydrogen-oxidising bacteria, we developed a sensitive and reliable reverse-transcriptase qpcr assay for up-regulation of the hups gene in the knallgas bacterium alcaligenes hydrogenophilus dsm2625. the assay detected up-regulation of the gene at initial hydrogen concentrations as low as 0 ... | 2011 | 21617984 |