| characterization of vibrio cholerae bacteriophage k139 and use of a novel mini-transposon to identify a phage-encoded virulence factor. | temperate bacteriophage k139 was isolated from a vibrio cholerae o139 isolate and characterized in this study. the phage genome consists of a 35 kbp, double-stranded, linear dna molecule that circularizes and integrates into the chromosome in a site-specific manner. dna sequences that cross-hybridize with k139 phage dna are present in all strains of v. cholerae serogroup o1 of the classical biotype examined and in some strains of the el tor biotype. phage k139 produces plaques on el tor o1 strai ... | 1995 | 8817491 |
| [role of moderate bacteriophage 139 in change in production of cholera toxin in a classic strain of vibrio cholerae]. | new data were obtained concerning cell sensitivity of pathogenic strains of cholera vibrions, which belong to the serogroup o1 of classical biovar, to the temperate bacteriophage k139, the native host of which is vibrio cholerae o139. molecular-genetic and biochemical studies showed that phage 139 integrated into the chromosome of strains v. cholerae o1 can change their toxigenic properties. a change in the production of cholera toxin (ct) in lysogens is associated both with an increase in the a ... | 2004 | 15174276 |
| temperate bacteriophage phio18p from an aeromonas media isolate: characterization and complete genome sequence. | a group of 74 aeromonas isolates from surface water of three ponds in bielefeld, germany was screened for prophage induction after uv irradiation. the phage phio18p was induced from the aeromonas media isolate o18. phio18p belongs to the myoviridae phage family. the complete nucleotide sequence of the double stranded dna genome of bacteriophage phio18p consists of 33,985 bp. the genome has 5' protruding cohesive ends of 16 bases. on the phio18p genome 46 open reading frames (orfs) were identifie ... | 2008 | 18096197 |
| characterization of vibrio cholerae o1 antigen as the bacteriophage k139 receptor and identification of is1004 insertions aborting o1 antigen biosynthesis. | bacteriophage k139 was recently characterized as a temperate phage of o1 vibrio cholerae. in this study we have determined the phage adsorption site on the bacterial cell surface. phage-binding studies with purified lipopolysaccharide (lps) of different o1 serotypes and biotypes revealed that the o1 antigen serves as the phage receptor. in addition, phage-resistant o1 el tor strains were screened by using a virulent isolate of phage k139. analysis of the lps of such spontaneous phage-resistant m ... | 2000 | 10960093 |
| vibrio cholerae phage k139: complete genome sequence and comparative genomics of related phages. | in this report, we characterize the complete genome sequence of the temperate phage k139, which morphologically belongs to the myoviridae phage family (p2 and 186). the prophage genome consists of 33,106 bp, and the overall gc content is 48.9%. forty-four open reading frames were identified. homology analysis and motif search were used to assign possible functions for the genes, revealing a close relationship to p2-like phages. by southern blot screening of a vibrio cholerae strain collection, t ... | 2002 | 12426348 |
| characterization of vibrio cholerae o1 el tor galu and gale mutants: influence on lipopolysaccharide structure, colonization, and biofilm formation. | recently we described the isolation of spontaneous bacteriophage k139-resistant vibrio cholerae o1 el tor mutants. in this study, we identified phage-resistant isolates with intact o antigen but altered core oligosaccharide which were also affected in galactose catabolism; this strains have mutations in the galu gene. we inactivated another gal gene, gale, and the mutant was also found to be defective in the catabolism of exogenous galactose but synthesized an apparently normal lipopolysaccharid ... | 2001 | 11119535 |
| characterization of the major control region of vibrio cholerae bacteriophage k139: immunity, exclusion, and integration. | the temperate bacteriophage k139 is highly associated with pathogenic o1 vibrio cholerae strains. the nucleotide sequence of the major control region of k139 was determined. the sequences of four (cox, cii, ci, and int) of the six deduced open reading frames and their gene order indicated that k139 is related to the p2 bacteriophage family. two genes of the lysogenic transcript from the mapped promoter pl encode homologs to the proteins ci and int, with deduced functions in prophage formation an ... | 1999 | 10217785 |