| new polyenic antibiotics active against gram-positive and gram-negative bacteria. iv. structural elucidation of enacyloxin iia. | the chemical structure of a unique polyenic antibiotic enacyloxin iia (former name: fr. 2) produced by frateuria (formerly gluconobacter) sp. w-315 has been determined by extensive spectroscopic studies, in particular by nmr spectral analysis. it has a novel non-lactonic structure involving 3,4-dihydroxycyclohexanecarboxylic acid with a chlorine-containing polyenic and polyhydroxy acyl side chain attached as an ester to the 3-hydroxyl substituent of the acid. | 1992 | 1592680 |
| differentiation between xanthomonas campestris pv. oryzae, xanthomonas campestris pv. oryzicola and the bacterial 'brown blotch' pathogen on rice by numerical analysis of phenotypic features and protein gel electrophoregrams. | thirty-five xanthomonas campestris pv. oryzae, fourteen x. campestris pv. oryzicola strains and six 'brown blotch' pathogens of rice, all of different geographical origin, were studied by numerical analysis of 133 phenotype features and gel electrophoregrams of soluble proteins, %g + c determinations and dna:rrna hybridizations. the following conclusions were drawn. (i) the xanthomonas campestris pathovars oryzae and oryzicola display clearly distinct protein patterns on polyacrylamide gels and ... | 1984 | 6084704 |
| dyella ginsengisoli sp. nov., isolated from soil of a ginseng field in south korea. | a gram-negative, aerobic, yellow-pigmented, non-spore-forming, motile, rod-shaped bacterium, designated strain gsoil 3046(t), was isolated from soil from a ginseng field in pocheon province, south korea, and was characterized taxonomically by using a polyphasic approach. a comparative analysis of 16s rrna gene sequences revealed that strain gsoil 3046(t) belongs to the family xanthomonadaceae in the gammaproteobacteria. the greatest sequence similarity was found with respect to dyella koreensis ... | 2009 | 19244422 |
| dyella japonica gen. nov., sp. nov., a gamma-proteobacterium isolated from soil. | three strains isolated from the soil of a garden in tokyo, japan, were characterized physiologically, biochemically and in terms of fatty acid profile, dna-dna relatedness and 16s rrna gene sequence. the isolates were gram-negative, aerobic, rod-shaped cells with polar flagellation. according to dna-dna similarity, the strains belonged to the same species. the bacteria grew at temperatures from 10 to 37 degrees c, with an optimum around 25-30 degrees c. growth was observed at ph values from 5.6 ... | 2005 | 15774657 |
| cloning and functional analysis of aniline dioxygenase gene cluster, from frateuria species ana-18, that metabolizes aniline via an ortho-cleavage pathway of catechol. | genes encoding an aniline dioxygenase of frateuria sp. ana-18, which metabolizes aniline via the ortho-cleavage pathway of catechol, were cloned and named tdn genes. the tdn genes were located on the chromosomal dna of this bacterium and weren't clustered with catechol-degrading gene clusters. these results show that the ana-18 aniline-degrading gene cluster is constructionally different from pseudomonas tdn and acinetobacter atd gene clusters, which degrade aniline via the meta-cleavage pathway ... | 2003 | 14646193 |
| fulvimonas soli gen. nov., sp. nov., a gamma-proteobacterium isolated from soil after enrichment on acetylated starch plastic. | six deep-yellow-pigmented strains were isolated from soil after enrichment on plasticized acetylated starch granules as a source of carbon. they showed very similar and unique fatty acid profiles, consisting almost exclusively of branched fatty acids. the strains consisted of small, motile rods, were oxidase- and catalase-positive, did not ferment sugars and were able to depolymerize starch and suspended acetylated starch in overlayer plates. the 16s rdna sequence of a representative strain, str ... | 2002 | 12148641 |
| aciduric proteobacteria isolated from ph 2.9 soil. | acidic (ph 2.9) soil was used as an inoculum to culture heterotrophic bacteria at ph values of 3-4. four isolates were obtained; on the basis of 16s rdna sequence, they were shown to be members of the beta- and gamma-proteobacteria. the three isolates that were most closely related to burkholderia spp. had simple nutritional requirements and could grow in glucose-mineral salts media; two of these used a broad array of organic substrates. the 16s rdna sequence of the fourth isolate was most simil ... | 2002 | 12070771 |
| isolation and phylogenetic characterization of acidophilic microorganisms indigenous to acidic drainage waters at an abandoned norwegian copper mine. | the biodiversity of culturable acidophilic microbes in three acidic (ph 2.7-3.7), metal-rich waters at an abandoned subarctic copper mine in central norway was assessed. acidophilic bacteria were isolated by plating on selective solid media, and dominant isolates were identified from their physiological characteristics and 16s rrna gene sequences. the dominant iron-oxidizing acidophile in all three waters was an acidithiobacillus ferrooxidans-like eubacterium, which shared 98% 16s rdna identity ... | 2001 | 11722543 |
| 16s rdna analysis for characterization of denitrifying bacteria isolated from three agricultural soils. | bacteria capable of denitrification are spread among phylogenetically diverse groups. in the present investigation, molecular methods (amplified ribosomal dna restriction analysis (ardra) and partial 16s rdna gene sequencing) were used to determine the genetic diversity of culturable denitrifying soil bacteria. the purpose of this work was to study the microbial density and diversity of denitrifying communities isolated from two luvisols and a rendosol. the denitrifying bacterial density was sig ... | 2000 | 11102689 |
| cloning and sequence analysis of two catechol-degrading gene clusters from the aniline-assimilating bacterium frateuria species ana-18. | the aniline-assimilating bacterium frateuria species ana-18 produced two catechol 1,2-dioxygenases, cd i and cd ii, and two muconate cycloisomerases, mc i and mc ii. the cata genes cata1 and cata2 encoding cd i and cd ii, respectively, were cloned from a gene library of this bacterium. the cata1 gene was clustered with catb1 encoding mc i, catc1 encoding muconolactone isomerase (mi), catd encoding beta-ketoadipate enol-lactone hydrolase (elh), and orfr1 encoding a putative lysr-type regulator. t ... | 1999 | 9931486 |
| purification and characterization of two muconate cycloisomerase isozymes from aniline-assimilating frateuria species ana-18. | two muconate cycloisomerases (mc i and mc ii, ec 5.5.1.1) were purified to homogeneity from an aniline-grown frateuria sp. ana-18. mc i and mc ii were similar in molecular mass, optimal ph, and ph stability but different in thermostability, and some other enzymatic properties. nh2-terminal amino acid sequences were different between the two isozymes, indicated that these are encoded by different genes. different inducible production of mc i and mc ii suggested that two catechol branches involved ... | 1998 | 9692194 |
| an extracellular quinoprotein oxidase that catalyzes conversion of enacyloxin iva to enacyloxin iia. | a new extracellular quinoprotein oxidase named enacyloxin oxidase (enx oxidase), which is involved in biosynthesis of enx iia, a congener of enx, was found in the culture supernatant of frateuria sp. w-315 and purified as a homogeneous protein on sds-page. enx oxidase was shown to have a molecular mass of 73 kda by sds-page and 79 kda by gel filtration. the enzyme was inhibited by various carbonyl reagents and the activity was stimulated by addition of pqq. this is the first report on a quinopro ... | 1994 | 7765518 |
| the nitrogen requirements of gluconobacter, acetobacter and frateuria. | the nitrogen requirements of 96 gluconobacter, 55 acetobacter and 7 frateuria strains were examined. only some frateuria strains were able to grow on 0.5% yeast extract broth or 0.5% peptone broth. in the presence of d-glucose or d-mannitol as a carbon source, ammonium was used as the sole source of nitrogen by all three genera. with ethanol, only a few acetobacter strains grew on ammonium as a sole nitrogen source. single l-amino acids cannot serve as a sole source of carbon and nitrogen for gr ... | 1981 | 7342881 |
| new polyenic antibiotics active against gram-positive and gram-negative bacteria. viii. construction of synthetic medium for production of mono-chloro-congeners of enacyloxins. | new antibiotics enacyloxins (enxs) are a family of non-lactonic polyene antibiotics produced by frateuria sp. w-315. for the production of antibiotics, we had to employ two-step fermentations, the first is the production of spent medium of neurospora crassa and the second is the production of antibiotics by frateuria. to simplify the production of antibiotics, systematic analyses have been done on the spent medium, and factors which affect the production of antibiotics characterized. from the ab ... | 1992 | 1534321 |
| dyella thiooxydans sp. nov., a facultatively chemolithotrophic, thiosulfate-oxidizing bacterium isolated from rhizosphere soil of sunflower (helianthus annuus l.). | a gram-negative, aerobic, motile, rod-shaped, thiosulfate-oxidizing bacterium, designated atsb10(t), was isolated from rhizosphere soil of sunflower (helianthus annuus l.). phylogenetic analysis based on 16s rrna gene sequences revealed that strain atsb10(t) was closely related to members of the genera dyella (96.4-98.1 % 16s rrna gene sequence similarity) and luteibacter (96.4-97.0 %) and fulvimonas soli lmg 19981(t) (96.7 %) and frateuria aurantia ifo 3245(t) (97.8 %). the predominant fatty ac ... | 2011 | 20305058 |
| influence of the temperature and the growth phase on the hopanoids and fatty acids content of frateuria aurantia (dsmz 6220). | the main lipids isolated from frateuria aurantia (dsmz 6220) are iso-branched fatty acids and triterpenoids of the hopane family like bacteriohopanetetrol and derived hopanoids, beside trace amounts of diploptene and rearranged compounds like fern-7-ene. the impact of the growth temperature and the growth phase in which cells were harvested on this lipid fingerprint was investigated. as expected, an increase of saturated compounds with temperature is the essential modification in the fatty acid ... | 2004 | 19712325 |
| genera and species in acetic acid bacteria. | taxonomic studies of acetic acid bacteria were historically surveyed. the genus acetobacter was first introduced in 1898 with a single species, acetobacter aceti. the genus gluconobacter was proposed in 1935 for strains with intense oxidation of glucose to gluconic acid rather than oxidation of ethanol to acetic acid and no oxidation of acetate. the genus "acetomonas" was described in 1954 for strains with polar flagellation and no oxidation of acetate. the proposals of the two generic names wer ... | 2008 | 18199517 |
| evidence that the potential for dissimilatory ferric iron reduction is widespread among acidophilic heterotrophic bacteria. | nineteen characterized strains and isolates of acidophilic heterotrophic bacteria were screened for their abilities to catalyse the reductive dissolution of the ferric iron mineral schwertmannite, under oxygen-limiting conditions. acidocella facilis, acidobacterium capsulatum, and all of the acidiphilium, acidocella and acidobacterium-like isolates that grew in liquid cultures were able to reduce iron. in contrast, neither acidisphaera rubrifaciens nor three acidisphaera-like isolates tested wer ... | 2008 | 18081844 |
| genus-specific profile of acetic acid bacteria by 16s rdna pcr-dgge. | an effective method for grouping acetic acid bacteria (aab) genera was defined and evaluated as a tool for preliminary screening of the major aab species involved in vinegar production. acetobacter, gluconobacter, gluconacetobacter, asaia, neoasaia, saccharibacter, frateuria and kozakia aab strains were screened on the basis of the 16s rdna sequences using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) technique. the dgge profile of all the strains tested, consisted ... | 2008 | 17919758 |
| stereochemistry of enacyloxins. part 4: complete structural and configurational assignment of the enacyloxin family, a series of antibiotics from frateuria sp. w-315. | the absolute configurations of the enacyloxins, a series of polyhydroxylated polyene antibiotics from frateuria species, were determined. the so far elusive (13'r,14'r,15's)-configuration was assigned to enacycloxin iva (1) by means of 'j-resolved hmbc-1' and noesy nmr methods. | 2007 | 17638342 |
| linear 3-hydroxybutyrate tetramer (hb4) produced by sphingomonas sp. is characterized as a growth promoting factor for some rhizomicrofloral composers. | sphingomonas spp. of alpha-proteobacteria often play a role in assisting the development of microfloral communities under adverse soil conditions. using a frateuria sp. as an indicator for bacterial growth assay, we investigated the bacterial growth-promoting factor in the culture fluids of sphingomonas sp. ec-k085. this factor was successfully isolated and identified as linear (r,r,r,r)-3-hydroxybutyrate tetramer (hb4), having a hydroxy-end and a carboxy-end group. when 28 mug of hb4 was charge ... | 2006 | 16960351 |
| dyella yeojuensis sp. nov., isolated from greenhouse soil in korea. | a novel strain, r2a16-10(t), was isolated from greenhouse soil in yeoju, korea. the taxonomy of strain r2a16-10(t) was studied by using polyphasic methods. on the basis of 16s rrna gene sequence analyses, strain r2a16-10(t) was found to be phylogenetically related to type strains of dyella species (96.7-96.9 %), frateuria aurantia dsm 6220(t) (96.5 %), fulvimonas soli lmg 19981(t) (96.3 %) and rhodanobacter species (94.9-95.7 %). strain r2a16-10(t), which produced yellow-coloured colonies, was g ... | 2006 | 16957102 |
| rhodanobacter spathiphylli sp. nov., a gammaproteobacterium isolated from the roots of spathiphyllum plants grown in a compost-amended potting mix. | three gram-negative, yellow-pigmented strains were isolated from the rhizospheres of spathiphyllum plants grown in a compost-amended potting mix. the strains showed biological control activity towards the root-rot plant pathogen cylindrocladium spathiphylli, and were characterized to determine their taxonomic position. cells of the strains were non-motile rods, and the strains were oxidase- and catalase-positive and unable to ferment most sugars tested. the three strains showed differences in gr ... | 2006 | 16902003 |
| luteibacter rhizovicinus gen. nov., sp. nov., a yellow-pigmented gammaproteobacterium isolated from the rhizosphere of barley (hordeum vulgare l.). | three strains of gram-negative, aerobic, yellow-pigmented, chemo-organotrophic bacteria, motile by a polar flagellum, were isolated from the rhizosphere of spring barley (hordeum vulgare l.) at a research field near copenhagen, denmark. the three strains, lj79, lj96t and lj99, formed visible colonies on one-tenth-strength tryptic soy broth supplemented with agar (1/10 tsba) after incubation for 6 days at 15 degrees c. the strains hydrolysed starch, casein (skimmed milk), gelatin and various pent ... | 2005 | 16280484 |
| dyella koreensis sp. nov., a beta-glucosidase-producing bacterium. | a bacterial strain (designated bb4(t)), which has beta-glucosidase activity, was isolated from soil around the roots of bamboo plants. cells were gram-negative, aerobic, non-motile and straight-rod-shaped. phylogenetic analysis of 16s rrna gene sequences revealed a clear affiliation with members of the family 'xanthomonadaceae'. the 16s rrna gene sequence of strain bb4(t) showed the following sequence similarities: 97.7% to dyella japonica xd53(t), 97.1% to frateuria aurantia lmg 1558(t), 96.2% ... | 2005 | 16014492 |
| aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of assam, india. | a bacterial strain designated gptsa 20(t), which was isolated from a warm spring in assam, india, was characterized by using a polyphasic approach. the cells were gram-negative, aerobic rods, which could not utilize or produce acid from most of the carbohydrates tested. the predominant fatty acids were c(15:0) iso (25.04%), c(17:1) iso omega9c (19.28%), c(16:0) iso (17.73%) and c(11:0) iso 3-oh (9.34%). the g+c content was 75 mol%. from 16s rrna gene sequence analysis (1433 nucleotides, continuo ... | 2005 | 16014470 |
| frateuria terrea sp. nov., isolated from forest soil, and emended description of the genus frateuria. | a bacterial strain, designated va24(t), was isolated from forest soil of the changbai mountains, heilongjiang province, china. cells of strain va24(t) were gram-reaction-negative, aerobic, non-spore-forming, long rods, 0.3-0.5×2.0-3.0 μm in size and were motile by means of a subpolar flagellum. strain va24(t) was oxidase-positive and catalase-negative. growth occurred at 21-36 °c, ph 5-10 and in 0-2 % (w/v) nacl but did not occur at 37 °c. the predominant respiratory quinone was q-8, the major p ... | 2011 | 20348318 |
| improvement of rna-sip by pyrosequencing to identify putative 4-n-nonylphenol degraders in activated sludge. | nonylphenols (np) have estrogenic potential because of their phenolic ring, but the organisms involved in the degradation of this alkylated phenol remain unidentified. using 16s ribosomal rna (rrna)-based stable isotope probing (sip) and a new method based on pyrosequencing, we identified the bacteria involved in the degradation of the aromatic ring of [u-ring-(13)c] 4-n-np in aerobic sludge. the first order degradation rate of 4-n-np was 5.5d(-1). single strand conformation polymorphism of dens ... | 2011 | 22154106 |
| a comparative study of changes in the microbiota of apple juice treated by high hydrostatic pressure (hhp) or high pressure homogenisation (hph). | the objective of this study was to assess the effect of high pressure homogenisation (hph) compared with high hydrostatic pressure (hhp) on the microbiological quality of raw apple juice during storage at ideal (4 °c) and abuse (12 °c) temperatures. in the case of hph, only low numbers of micro-organisms were detected after treatment at 300 mpa (typically between 2 and 3 log.ml⁻¹). these were identified as streptomyces spp., and numbers did not increase during storage of the juice for 35 days, i ... | 2011 | 21925024 |
| regulation by two catr proteins that differ in binding affinity to catb promoters expressing two cat gene clusters. | we isolated the two lysr-type regulatory proteins catr1 and catr2, which regulate the expression of cat1 and cat2 gene clusters, respectively, required for catechol degradation in the bacterium frateuria sp. ana-18. in a gel mobility shift assay using catr1 and the dna fragment containing the catb1 promoter region, the formation of two complexes, complex 1-1 (c1-1) and complex 1-2 (c1-2), was observed in the presence of cis,cis-muconate. on the other hand, catr2 and the dna fragment containing t ... | 2001 | 11758902 |
| identification of acetic acid bacteria by rflp of pcr-amplified 16s rdna and 16s-23s rdna intergenic spacer. | dna corresponding to 16s rdna and the 165-23s rdna intergenic spacer (its) from 22 reference strains of acetic acid bacteria, representing the diversity of the family acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by pcr-rflp. frateuria aurantia lmg 1558t and escherichia coli atcc 11775t were included as outgroups. pcr-amplified products of about 1450 bp were obtained from the 16s rdna of all the strains and products of between 675 and 800 ... | 2000 | 11155971 |