| sequence of rna 2 of a nematode-transmissible isolate of tobacco rattle virus. | tobacco rattle virus (trv) isolate ppk20 is transmitted by paratrichodorus pachydermus nematodes. the factor(s) determining vector transmissibility has been shown to be located on trv rna 2. sequence determination revealed that ppk20 rna 2 contains three open reading frames encoding the coat protein (cp) and proteins with molecular masses of 29.4 and 32.8 kda. the 29.4 and 32.8 kda protein-coding genes showed no significant sequence similarity to any other known tobravirus gene. a full-length cd ... | 1995 | 7595393 |
| transmission of tobacco rattle virus isolate ppk20 by its nematode vector requires one of the two non-structural genes in the viral rna 2. | tobacco rattle virus isolate ppk20 is transmitted by the nematode paratrichodorus pachydermus. rna 2 of the virus determines vector transmissibility and encodes the viral coat protein and two non-structural proteins with molecular masses of 29.4 kda and 32.8 kda. deletions and a frameshift in the two non-structural genes did not interfere with encapsidation or co-replication of rna 2 with rna 1 after mechanical inoculation of plants. mutations that affected the 29.4k gene or both non-structural ... | 1997 | 9018070 |
| similarities in the genome organization of tobacco rattle virus and pea early-browning virus isolates that are transmitted by the same vector nematode. | although sequence data have been obtained for several tobravirus isolates, only two of these isolates are nematode-transmissible. tobacco rattle virus (trv) ppk20 is transmitted by paratrichodorus pachydermus, whereas pea early-browning virus (pebv) tpa56 is transmitted by trichodorus primitivus. to clarify whether differences in the genome structure of these isolates are relevant to the specificity of interactions with particular vector nematodes, or merely reflect a taxonomic difference betwee ... | 1999 | 9934712 |
| nematode transmission of tobacco rattle virus serves as a bottleneck to clear the virus population from defective interfering rnas. | di7 is a defective interfering rna derived from rna 2 of tobacco rattle tobravirus (trv) isolate ppk20. tobacco was transformed with di7 cdna fused to the camv 35s promoter. upon infection of the transgenic plants with trv isolate ppk20 or the serologically unrelated isolate pay4, the transgenic di7 rna started to accumulate at high levels and strongly interfered with accumulation of wild-type (wt) rna 2. when di7 transgenic plants infected with isolate ppk20 were used as source plants in nemato ... | 1999 | 10544090 |
| nonstructural proteins of tobacco rattle virus which have a role in nematode-transmission: expression pattern and interaction with viral coat protein. | rna 2 of tobacco rattle virus isolate ppk20 encodes the viral coat protein (cp) and two nonstructural proteins of 40 kda ('40k protein') and 32.8 kda ('32.8k'). the 40k protein is required for transmission of the virus by the vector nematode paratrichodorus pachydermus whereas the 32.8k protein may be involved in transmission by other vector nematode species. an antiserum was raised against the 40k protein expressed in e. coli and used to study the expression and subcellular localization of this ... | 1999 | 10567661 |
| tobravirus 2b protein acts in trans to facilitate transmission by nematodes. | analysis of rna2 of trv pay4 showed it to be recombinant, carrying 3'-terminal sequences derived from rna1. virus produced using an infectious cdna clone of pay4 rna2 was nematode transmissible, demonstrating that natural trv recombinant isolates are not necessarily defective. mutations introduced into pay4 rna2 showed that the 2b gene, but not the 2c gene, is required for transmission by both paratrichodorus pachydermus and p. anemones nematodes. experiments examined whether infection of plants ... | 2001 | 11162804 |
| real-time pcr detection and quantification of vector trichodorid nematodes and tobacco rattle virus. | this report describes a novel diagnostic method for virus-vector trichodorid nematodes and associated tobacco rattle virus (trv) based on a real-time fluorogenic 5' nuclease pcr assay (taqman). two independent primer/probe sets were designed targeting the 18s gene of the ribosomal cistron for the trichodorid species, paratrichodorus pachydermus and trichodorus similis. assays using purified plasmid dna containing clones of the 18s region and genomic dna extracted from individuals from both nemat ... | 2006 | 16563695 |
| yeast two-hybrid study of tobacco rattle virus coat protein and 2b protein interactions. | the specificity of the interaction between the coat protein (cp) and 2b nematode-transmission helper protein of two isolates, ppk20 and pay4, of tobacco rattle virus (trv) that differ in their transmission characteristics was investigated. yeast two-hybrid experiments identified a central domain of the cp that promoted cp:cp interaction but inhibited cp:2b interaction. deletion of a conserved coiled-coil domain from the 2b protein prevented its interaction with cp, whereas deletion of n- and c-t ... | 2006 | 16763731 |
| some aspects of the natural control of plant parasitic nematodes in soil under broad bean vicia faba l. cultivated in crop rotation and long-term monoculture. | observations on population density of plant parasitic nematodes occurring in rhizosphere of broad bean cultivated in the crop rotation and long-term monoculture were performed during 2013-2014. 13 species were observed: trichodorus primitivus, t. viruliferus, paratrichodorus pachydermus, criconema annuliferum, paratylenchus projectus, bitylenchus dubius, merlinius brevidens, pratylenchus fallax, p. flakkensis, p. neglectus, heterodera triffolii, h. goettingiana, and ditylenchus dipsaci. in monoc ... | 2015 | 27145570 |