real-time pcr detection and quantification of vector trichodorid nematodes and tobacco rattle virus.this report describes a novel diagnostic method for virus-vector trichodorid nematodes and associated tobacco rattle virus (trv) based on a real-time fluorogenic 5' nuclease pcr assay (taqman). two independent primer/probe sets were designed targeting the 18s gene of the ribosomal cistron for the trichodorid species, paratrichodorus pachydermus and trichodorus similis. assays using purified plasmid dna containing clones of the 18s region and genomic dna extracted from individuals from both nemat ...200616563695
some aspects of the natural control of plant parasitic nematodes in soil under broad bean vicia faba l. cultivated in crop rotation and long-term monoculture.observations on population density of plant parasitic nematodes occurring in rhizosphere of broad bean cultivated in the crop rotation and long-term monoculture were performed during 2013-2014. 13 species were observed: trichodorus primitivus, t. viruliferus, paratrichodorus pachydermus, criconema annuliferum, paratylenchus projectus, bitylenchus dubius, merlinius brevidens, pratylenchus fallax, p. flakkensis, p. neglectus, heterodera triffolii, h. goettingiana, and ditylenchus dipsaci. in monoc ...201527145570
similarities in the genome organization of tobacco rattle virus and pea early-browning virus isolates that are transmitted by the same vector nematode.although sequence data have been obtained for several tobravirus isolates, only two of these isolates are nematode-transmissible. tobacco rattle virus (trv) ppk20 is transmitted by paratrichodorus pachydermus, whereas pea early-browning virus (pebv) tpa56 is transmitted by trichodorus primitivus. to clarify whether differences in the genome structure of these isolates are relevant to the specificity of interactions with particular vector nematodes, or merely reflect a taxonomic difference betwee ...19999934712
sequence comparison of rna2 of nematode-transmissible and nematode-non-transmissible isolates of pea early-browning virus suggests that the gene encoding the 29 kda protein may be involved in nematode transmission.a cdna clone containing the complete coding region of rna2 of the newly described tpa56 isolate of pea early-browning virus (english serotype, pebv-e) has been obtained. a plasmid (pfla56) containing this clone together with the 5' and 3' non-coding regions of pebv isolate sp5 (the type isolate of pebv-e) and flanked by the camv 35s promoter and nos terminator is infectious when co-inoculated onto plants with pcan1, an infectious clone of pebv sp5 rna1. virus containing rna2 derived from the cdn ...19957730817
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