| a diagnostic oligonucleotide microarray for simultaneous detection of grapevine viruses. | at least 58 viruses have been reported to infect grapevines causing economic damage globally. conventional detection strategies based on serological assays, biological indexing and rt-pcr targeting one or few viruses in each assay are widely used. grapevines are prone to contain mixed infections of several viruses, making the use of these techniques time-consuming. a 70-mer oligonucleotide microarray able to detect simultaneously a broad spectrum of known viruses as well as new viruses by cross- ... | 2010 | 19914293 |
| molecular characterization of a grapevine leafroll-associated virus 4 from slovenian vineyards. | during a survey conducted in vineyards in slovenia, variety of grapevine leafroll disease symptoms were observed. mixed infection with grapevine leafroll-associated viruses 3 and 4 (glrav-3, -4) in two grapevines from a vineyard in south-western part of slovenia was confirmed by das-elisa in 2010. the 3'final 1769 nucleotides of the slovenian glrav-4 isolate were assembled from amplicons obtained by ic rt-pcr. the complete coat protein (cp) and p23 gene sequences were compared with other glrav-4 ... | 2016 | 27265467 |
| biological, serological, and molecular characterization of a highly divergent strain of grapevine leafroll-associated virus 4 causing grapevine leafroll disease. | the complete genome sequence of a highly divergent strain of grapevine leafroll-associated virus 4 (glrav-4) was determined using 454 pyrosequencing technology. this virus, designated glrav-4 ob, was detected in vitis vinifera 'otcha bala' from our grapevine virus collection at agroscope. the glrav-4 ob genome length and organization share similarities with members of subgroup ii in the genus ampelovirus (family closteroviridae). otcha bala was graft-inoculated onto indicator plants of cultivar ... | 2015 | 25822183 |
| novel variants of grapevine leafroll-associated virus 4 and 7 detected from a grapevine showing leafroll symptoms. | | 2013 | 22926688 |
| genome organization, serology and phylogeny of grapevine leafroll-associated viruses 4 and 6: taxonomic implications. | complete nucleotide sequences of the type isolate of grapevine leafroll-associated virus 4 (glrav-4) and of an isolate of glrav-6 from cv 'estellat' (glrav-6est) were generated and compared mutually and with related viruses. the genome organization of both viruses resembled that of members of subgroup i in the genus ampelovirus (fam. closteroviridae). the availability of these sequences, along with previously existing data on related glravs, allowed critical review of the taxonomy and nomenclatu ... | 2012 | 21925555 |
| use of degenerate primers for partial sequencing and rt-pcr-based assays of grapevine leafroll-associated viruses 4 and 5. | abstract double-stranded rna (dsrna) was purified from grapevines infected with grapevine leafroll-associated viruses 4 (glrav-4) and 5 (glrav-5), two putative closteroviruses. reverse-transcriptase polymerase chain reaction (rt-pcr) was performed on this dsrna using degenerate oligonucleotides designed to amplify an approximately 550- to 650-nucleotide fragment from the heat shock protein 70 homolog (hsp70) of the known closteroviruses. rt-pcr products of the appropriate molecular weight were g ... | 1998 | 18944860 |
| generic and species-specific detection of viruses belonging to an evolutionary distinct lineage within the ampelovirus genus. | a nested rt-pcr was developed, that allows the generic detection of a subgroup of genetically related viruses with a distinct evolutionary history within the genus ampelovirus. members of this lineage are grapevine leafroll associated virus-4, -5, -6, -9 and two isolates (glrav-de and glrav-pr) that have been recently characterized and represent new species. the method involves a one step rt-pcr for the generic detection of closteroviridae species using degenerate primers that target the hsp70h ... | 2008 | 18848581 |
| efficient cloning of cdna from grapevine leafroll-associated virus 4 and demonstration of probe specificity by the viral antibody. | using a random-pcr method, a cdna clone (lr4) was constructed from the replicative form dsrna of grapevine leafroll-associated virus 4 (glrav-4). northern blot analysis showed hybridization of lr4 to dsrna in an extract of a thompson seedless grapevine clone from which glrav-4 was isolated originally by hu et al. (1990). the cdna clone was sequenced and shown to be specific to glrav-4 by reverse-transcription-pcr using glrav-4 particles enriched by the virus antibody coupled to magnetic beads. r ... | 1998 | 9562414 |